Human Gene LIN9 (uc001hqa.3)
  Description: Homo sapiens lin-9 homolog (C. elegans) (LIN9), transcript variant 1, mRNA.
RefSeq Summary (NM_173083): This gene encodes a tumor suppressor protein that inhibits DNA synthesis and oncogenic transformation through association with the retinoblastoma 1 protein. The encoded protein also interacts with a complex of other cell cycle regulators to repress cell cycle-dependent gene expression in non-dividing cells. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012].
Transcript (Including UTRs)
   Position: hg19 chr1:226,418,850-226,497,204 Size: 78,355 Total Exon Count: 15 Strand: -
Coding Region
   Position: hg19 chr1:226,420,202-226,496,888 Size: 76,687 Coding Exon Count: 15 

Page IndexSequence and LinksPrimersGenetic AssociationsMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:226,418,850-226,497,204)mRNA (may differ from genome)Protein (558 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMalacards
MGIOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): LIN9
CDC HuGE Published Literature: LIN9

-  MalaCards Disease Associations
  MalaCards Gene Search: LIN9
Diseases sorted by gene-association score: algoneurodystrophy (4), tuberculous peritonitis (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.75 RPKM in Testis
Total median expression: 46.03 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -180.10316-0.570 Picture PostScript Text
3' UTR -302.651352-0.224 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF06584 - DIRP

ModBase Predicted Comparative 3D Structure on Q5TKA1-2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
  Ensembl   
  Protein Sequence   
  Alignment   

-  Descriptions from all associated GenBank mRNAs
  AF190323 - Homo sapiens type I interferon receptor beta chain-associated protein splice variant (BARA) mRNA, complete cds, alternatively spliced.
BC043444 - Homo sapiens lin-9 homolog (C. elegans), mRNA (cDNA clone IMAGE:5297824), partial cds.
BC065302 - Homo sapiens lin-9 homolog (C. elegans), mRNA (cDNA clone IMAGE:6156654), partial cds.
AF190324 - Homo sapiens type I interferon receptor beta chain-associated protein (BARA) mRNA, complete cds, alternatively spliced.
BX537869 - Homo sapiens mRNA; cDNA DKFZp313M1636 (from clone DKFZp313M1636).
BC045625 - Homo sapiens lin-9 homolog (C. elegans), mRNA (cDNA clone MGC:39574 IMAGE:4837217), complete cds.
AK126177 - Homo sapiens cDNA FLJ44189 fis, clone THYMU2039315, weakly similar to Caenorhabditis elegans LIN-9L (lin-9) mRNA.
AY786184 - Homo sapiens Lin-9 mRNA, complete cds.
KJ896024 - Synthetic construct Homo sapiens clone ccsbBroadEn_05418 LIN9 gene, encodes complete protein.
AK308823 - Homo sapiens cDNA, FLJ98864.
AY166859 - Homo sapiens TGS2 mRNA, complete cds.
AY166858 - Homo sapiens TGS1 mRNA, complete cds.
AK125690 - Homo sapiens cDNA FLJ43702 fis, clone TESOP2001122, weakly similar to Caenorhabditis elegans LIN-9S (lin-9) mRNA.
JD385870 - Sequence 366894 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q5TKA1 (Reactome details) participates in the following event(s):

R-HSA-1362270 Phosphorylation of LIN52 component of MuvB by DYRK1A
R-HSA-4088306 FOXM1 binds PLK1 promoter
R-HSA-4088307 FOXM1 binds CCNB1 promoter
R-HSA-4088309 FOXM1 binds CCNB2 promoter
R-HSA-1362261 p130 (RBL2) associates with MuvB to form DREAM complex
R-HSA-8964465 DREAM complex binds the PCNA gene promoter
R-HSA-8964471 DREAM complex binds the CDC25A gene promoter
R-HSA-8964475 DREAM complex binds the TOP2A gene promoter
R-HSA-8964482 DREAM complex binds the E2F1 gene promoter
R-HSA-8964492 DREAM complex binds the RBL1 gene promoter
R-HSA-8964498 DREAM complex binds the CDC6 gene promoter
R-HSA-1538133 G0 and Early G1
R-HSA-156711 Polo-like kinase mediated events
R-HSA-453279 Mitotic G1-G1/S phases
R-HSA-69275 G2/M Transition
R-HSA-1362277 Transcription of E2F targets under negative control by DREAM complex
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-453274 Mitotic G2-G2/M phases
R-HSA-1640170 Cell Cycle

-  Other Names for This Gene
  Alternate Gene Symbols: BARA, NM_173083, NP_001257339, Q5TKA1-2, TGS, uc001hqa.2
UCSC ID: uc001hqa.3
RefSeq Accession: NM_173083
Protein: Q5TKA1-2, splice isoform of Q5TKA1 CCDS: CCDS1553.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_173083.3
exon count: 15CDS single in 3' UTR: no RNA size: 3345
ORF size: 1677CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3182.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.