Human Gene NCAPH2 (uc003blx.4)
  Description: Homo sapiens non-SMC condensin II complex, subunit H2 (NCAPH2), transcript variant 3, mRNA.
RefSeq Summary (NM_001185011): This gene encodes one of the non-SMC subunits of the condensin II complex. This complex plays an essential role in mitotic chromosome assembly. Alternate splicing of this gene results in multiple transcript variants.[provided by RefSeq, May 2010].
Transcript (Including UTRs)
   Position: hg19 chr22:50,946,645-50,963,209 Size: 16,565 Total Exon Count: 20 Strand: +
Coding Region
   Position: hg19 chr22:50,946,767-50,961,804 Size: 15,038 Coding Exon Count: 20 

Page IndexSequence and LinksPrimersGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:50,946,645-50,963,209)mRNA (may differ from genome)Protein (606 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
H-INVHGNCLynxMGIOMIMPubMed
ReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): NCAPH2
CDC HuGE Published Literature: NCAPH2

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 40.68 RPKM in Testis
Total median expression: 816.86 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -50.20122-0.411 Picture PostScript Text
3' UTR -597.461405-0.425 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF06278 - Condensin II complex subunit CAP-H2 or CNDH2, N-terminal
PF16858 - Condensin II complex subunit CAP-H2 or CNDH2, C-term
PF16869 - PF16858

ModBase Predicted Comparative 3D Structure on Q6IBW4-4
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologNo ortholog
      
      
  Ensembl   
  Protein Sequence   
  Alignment   

-  Descriptions from all associated GenBank mRNAs
  BC000473 - Homo sapiens non-SMC condensin II complex, subunit H2, mRNA (cDNA clone IMAGE:2961557), complete cds.
BC001509 - Homo sapiens non-SMC condensin II complex, subunit H2, mRNA (cDNA clone IMAGE:2961557), complete cds.
BC001833 - Homo sapiens non-SMC condensin II complex, subunit H2, mRNA (cDNA clone IMAGE:2961557), complete cds.
BC001298 - Homo sapiens non-SMC condensin II complex, subunit H2, mRNA (cDNA clone IMAGE:3459027), with apparent retained intron.
AL021682 - Homo sapiens cDNA mapping to 22q13.
BC001937 - Homo sapiens non-SMC condensin II complex, subunit H2, mRNA (cDNA clone MGC:4097 IMAGE:2819717), complete cds.
BC009441 - Homo sapiens non-SMC condensin II complex, subunit H2, mRNA (cDNA clone MGC:15858 IMAGE:3509636), complete cds.
CR456604 - Homo sapiens Em:U62317.2 full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.Em:U62317.2).
JD128858 - Sequence 109882 from Patent EP1572962.
AB527191 - Synthetic construct DNA, clone: pF1KE0343, Homo sapiens NCAPH2 gene for non-SMC condensin II complex, subunit H2, without stop codon, in Flexi system.
CU013373 - Homo sapiens Em:U62317.C22.2, mRNA (cDNA clone IMAGE:100000248), complete cds, without stop codon, in Gateway system.
CU013085 - Homo sapiens Em:U62317.C22.2, mRNA (cDNA clone IMAGE:100000344), complete cds, with stop codon, in Gateway system.
JD535376 - Sequence 516400 from Patent EP1572962.
JD380464 - Sequence 361488 from Patent EP1572962.
JD029645 - Sequence 10669 from Patent EP1572962.
KJ906136 - Synthetic construct Homo sapiens clone ccsbBroadEn_15806 NCAPH2 gene, encodes complete protein.
CU674650 - Synthetic construct Homo sapiens gateway clone IMAGE:100017396 5' read NCAPH2 mRNA.
CU674940 - Synthetic construct Homo sapiens gateway clone IMAGE:100017406 5' read NCAPH2 mRNA.
KJ906135 - Synthetic construct Homo sapiens clone ccsbBroadEn_15805 NCAPH2 gene, encodes complete protein.
BC014939 - Homo sapiens non-SMC condensin II complex, subunit H2, mRNA (cDNA clone IMAGE:3922217), partial cds.
JD180635 - Sequence 161659 from Patent EP1572962.
CU677121 - Synthetic construct Homo sapiens gateway clone IMAGE:100017312 5' read NCAPH2 mRNA.
KJ902509 - Synthetic construct Homo sapiens clone ccsbBroadEn_11903 NCAPH2 gene, encodes complete protein.
DQ579583 - Homo sapiens piRNA piR-47695, complete sequence.
JD451921 - Sequence 432945 from Patent EP1572962.
JD484650 - Sequence 465674 from Patent EP1572962.
JD539422 - Sequence 520446 from Patent EP1572962.
JD107122 - Sequence 88146 from Patent EP1572962.
JD108715 - Sequence 89739 from Patent EP1572962.
JD187657 - Sequence 168681 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q6IBW4 (Reactome details) participates in the following event(s):

R-HSA-2172666 RB1 binds condensin II
R-HSA-2288097 Condensin II complex binds H4K20me1-containing nucleosomes
R-HSA-2429719 MCPH1 sequesters condensin II
R-HSA-2294600 CDK1 phosphorylates condensin II subunit NCAPD3
R-HSA-2294580 PLK1 hyperphosphorylates Condensin II complex
R-HSA-2294590 PLK1 binds phosphorylated condensin II complex
R-HSA-2299718 Condensation of Prophase Chromosomes
R-HSA-68875 Mitotic Prophase
R-HSA-68886 M Phase
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-1640170 Cell Cycle

-  Other Names for This Gene
  Alternate Gene Symbols: CAPH2, NM_001185011, NP_001171940, Q6IBW4-4
UCSC ID: uc003blx.4
RefSeq Accession: NM_001185011
Protein: Q6IBW4-4, splice isoform of Q6IBW4 CCDS: CCDS14094.2, CCDS54546.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001185011.1
exon count: 20CDS single in 3' UTR: no RNA size: 3348
ORF size: 1821CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3812.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.