Mouse Gene Notch2 (ENSMUST00000079812.7) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus notch 2 (Notch2), mRNA. (from RefSeq NM_010928)
Gencode Transcript: ENSMUST00000079812.7
Gencode Gene: ENSMUSG00000027878.11
Transcript (Including UTRs)
   Position: mm10 chr3:98,013,538-98,150,361 Size: 136,824 Total Exon Count: 34 Strand: +
Coding Region
   Position: mm10 chr3:98,013,699-98,147,444 Size: 133,746 Coding Exon Count: 34 

Page IndexSequence and LinksUniProtKB CommentsCTDRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr3:98,013,538-98,150,361)mRNA (may differ from genome)Protein (2473 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeStanford SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Neurogenic locus notch homolog protein 2; Short=Notch 2; AltName: Full=Motch B; Contains: RecName: Full=Notch 2 extracellular truncation; Contains: RecName: Full=Notch 2 intracellular domain; Flags: Precursor;
FUNCTION: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation.
SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH2. Interacts with RELA/p65. Interacts with HIF1AN.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
SUBCELLULAR LOCATION: Notch 2 intracellular domain: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
TISSUE SPECIFICITY: Expressed in the brain, liver, kidney, neuroepithelia, somites, optic vesicles and branchial arches, but not heart.
DEVELOPMENTAL STAGE: Expressed in the embryonic ventricular zone, the postnatal ependymal cells, and the choroid plexus throughout embryonic and postnatal development.
PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
PTM: Hydroxylated by HIF1AN.
SIMILARITY: Belongs to the NOTCH family.
SIMILARITY: Contains 6 ANK repeats.
SIMILARITY: Contains 35 EGF-like domains.
SIMILARITY: Contains 3 LNR (Lin/Notch) repeats.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -85.90161-0.534 Picture PostScript Text
3' UTR -894.002917-0.306 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002110 - Ankyrin_rpt
IPR020683 - Ankyrin_rpt-contain_dom
IPR024600 - DUF3454_notch
IPR000742 - EG-like_dom
IPR001881 - EGF-like_Ca-bd
IPR013032 - EGF-like_CS
IPR000152 - EGF-type_Asp/Asn_hydroxyl_site
IPR018097 - EGF_Ca-bd_CS
IPR008297 - Notch
IPR022336 - Notch_2
IPR000800 - Notch_dom
IPR010660 - Notch_NOD_dom
IPR011656 - Notch_NODP_dom

Pfam Domains:
PF00008 - EGF-like domain
PF00023 - Ankyrin repeat
PF00066 - LNR domain
PF06816 - NOTCH protein
PF07645 - Calcium-binding EGF domain
PF07684 - NOTCH protein
PF11936 - Domain of unknown function (DUF3454)
PF12661 - Human growth factor-like EGF
PF12796 - Ankyrin repeats (3 copies)
PF13637 - Ankyrin repeats (many copies)
PF13857 - Ankyrin repeats (many copies)

SCOP Domains:
140860 - Pseudo ankyrin repeat-like
48403 - Ankyrin repeat
57196 - EGF/Laminin
57184 - Growth factor receptor domain
90193 - Notch domain

ModBase Predicted Comparative 3D Structure on O35516
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologGenome BrowserGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence Protein SequenceProtein Sequence 
AlignmentAlignment AlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005509 calcium ion binding
GO:0005515 protein binding
GO:0019899 enzyme binding
GO:0038023 signaling receptor activity
GO:0051059 NF-kappaB binding

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001701 in utero embryonic development
GO:0001709 cell fate determination
GO:0001889 liver development
GO:0001890 placenta development
GO:0001947 heart looping
GO:0002011 morphogenesis of an epithelial sheet
GO:0002315 marginal zone B cell differentiation
GO:0002437 inflammatory response to antigenic stimulus
GO:0003184 pulmonary valve morphogenesis
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006959 humoral immune response
GO:0007050 cell cycle arrest
GO:0007219 Notch signaling pathway
GO:0007275 multicellular organism development
GO:0007368 determination of left/right symmetry
GO:0008284 positive regulation of cell proliferation
GO:0009887 animal organ morphogenesis
GO:0010629 negative regulation of gene expression
GO:0030154 cell differentiation
GO:0030326 embryonic limb morphogenesis
GO:0030513 positive regulation of BMP signaling pathway
GO:0035264 multicellular organism growth
GO:0035622 intrahepatic bile duct development
GO:0042060 wound healing
GO:0042742 defense response to bacterium
GO:0043011 myeloid dendritic cell differentiation
GO:0043065 positive regulation of apoptotic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0045967 negative regulation of growth rate
GO:0046579 positive regulation of Ras protein signal transduction
GO:0046849 bone remodeling
GO:0050793 regulation of developmental process
GO:0060413 atrial septum morphogenesis
GO:0060674 placenta blood vessel development
GO:0061073 ciliary body morphogenesis
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0070986 left/right axis specification
GO:0072014 proximal tubule development
GO:0072015 glomerular visceral epithelial cell development
GO:0072104 glomerular capillary formation
GO:0072574 hepatocyte proliferation
GO:0072576 liver morphogenesis
GO:0072602 interleukin-4 secretion
GO:1990705 cholangiocyte proliferation
GO:2001204 regulation of osteoclast development

Cellular Component:
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0005929 cilium
GO:0009986 cell surface
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0043235 receptor complex

-  Descriptions from all associated GenBank mRNAs
  AK157200 - Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830206H20 product:Notch gene homolog 2 (Drosophila), full insert sequence.
D32210 - Mus musculus (Notch2) mRNA, complete cds.
BC172642 - Synthetic construct Mus musculus clone IMAGE:100069450, MGC:199352 Notch gene homolog 2 (Drosophila) (Notch2) mRNA, encodes complete protein.
X68279 - M.musculus motch B mRNA.
AK037135 - Mus musculus adult female vagina cDNA, RIKEN full-length enriched library, clone:9930124I24 product:Notch gene homolog 2, (Drosophila), full insert sequence.
AK155137 - Mus musculus NOD-derived CD11c +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F630203H03 product:Notch gene homolog 2 (Drosophila), full insert sequence.
AK155666 - Mus musculus B6-derived CD11 +ve dendritic cells cDNA, RIKEN full-length enriched library, clone:F730116C03 product:Notch gene homolog 2 (Drosophila), full insert sequence.
U31881 - Mus musculus Notch-2 mRNA, partial cds.
BC059256 - Mus musculus Notch gene homolog 2 (Drosophila), mRNA (cDNA clone IMAGE:6406444), complete cds.
AK027963 - Mus musculus 18-day embryo whole body cDNA, RIKEN full-length enriched library, clone:1110037M13 product:unclassifiable, full insert sequence.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04320 - Dorso-ventral axis formation
mmu04330 - Notch signaling pathway

Reactome (by CSHL, EBI, and GO)

Protein O35516 (Reactome details) participates in the following event(s):

R-NUL-2197556 Gamma-secretase complex cleaves mNEXT2
R-NUL-1986584 Fucosylation of Pre-Notch by POFUT1
R-MMU-2023179 Glucosylation of Pre-Notch by Poglut1
R-MMU-2023158 Glycosylation of Pre-Notch by Fringe
R-MMU-2032750 Sel1l negatively regulates Pre-Notch exit from endoplasmic reticulum
R-MMU-2032378 Jag1 binds Notch2
R-MMU-2032379 Jag2 binds Notch2
R-MMU-2060798 Dll1 binds Notch2
R-MMU-2976706 Notch2 and phosphorylated Creb1 bind Gzmb promoter
R-MMU-2976717 Notch2 binds Hes1 promoter
R-MMU-2976723 Notch2 binds Hes5 promoter
R-MMU-2193065 Adam10 cleaves Notch2

-  Other Names for This Gene
  Alternate Gene Symbols: NM_010928, NOTC2_MOUSE, O35516, Q06008, Q60941, uc008qpo.1, uc008qpo.2
UCSC ID: uc008qpo.2
RefSeq Accession: NM_010928
Protein: O35516 (aka NOTC2_MOUSE or NTC2_MOUSE)
CCDS: CCDS51013.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.