Mouse Gene Slc25a27 (ENSMUST00000024705.6) from GENCODE VM33
  Description: Mus musculus solute carrier family 25, member 27 (Slc25a27), transcript variant 1, mRNA. (from RefSeq NM_028711)
Gencode Transcript: ENSMUST00000024705.6
Gencode Gene: ENSMUSG00000023912.11
Transcript (Including UTRs)
   Position: mm39 chr17:43,952,782-43,978,105 Size: 25,324 Total Exon Count: 9 Strand: -
Coding Region
   Position: mm39 chr17:43,954,496-43,977,893 Size: 23,398 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Methods
Data last updated at UCSC: 2023-08-15 19:06:27

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:43,952,782-43,978,105)mRNA (may differ from genome)Protein (322 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: Q9D6D0_MOUSE
DESCRIPTION: SubName: Full=Protein Slc25a27; SubName: Full=Solute carrier family 25, member 27; SubName: Full=Solute carrier family 25, member 27, isoform CRA_a; SubName: Full=Uncoupling protein 4;
SIMILARITY: Belongs to the mitochondrial carrier family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -87.10212-0.411 Picture PostScript Text
3' UTR -529.701714-0.309 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018108 - Mitochondrial_sb/sol_carrier
IPR023395 - Mt_carrier_dom

Pfam Domains:
PF00153 - Mitochondrial carrier protein

ModBase Predicted Comparative 3D Structure on Q9D6D0
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
GenBankRGDEnsemblEnsemblWormBase 
Protein SequenceProtein SequenceProtein SequenceProtein SequenceProtein Sequence 
AlignmentAlignmentAlignmentAlignmentAlignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0022857 transmembrane transporter activity

Biological Process:
GO:0006839 mitochondrial transport
GO:0008284 positive regulation of cell proliferation
GO:0009409 response to cold
GO:0010917 negative regulation of mitochondrial membrane potential
GO:0035356 cellular triglyceride homeostasis
GO:0043066 negative regulation of apoptotic process
GO:0046324 regulation of glucose import
GO:0048839 inner ear development
GO:0051562 negative regulation of mitochondrial calcium ion concentration
GO:0051881 regulation of mitochondrial membrane potential
GO:0055085 transmembrane transport
GO:0070997 neuron death

Cellular Component:
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0031966 mitochondrial membrane
GO:0043025 neuronal cell body
GO:0045177 apical part of cell


-  Descriptions from all associated GenBank mRNAs
  AK083270 - Mus musculus adult male hippocampus cDNA, RIKEN full-length enriched library, clone:C630032J21 product:inferred: RIKEN cDNA 3632410G24 gene, full insert sequence.
AK084741 - Mus musculus 13 days embryo heart cDNA, RIKEN full-length enriched library, clone:D330037G19 product:inferred: RIKEN cDNA 3632410G24 gene, full insert sequence.
BC031371 - Mus musculus solute carrier family 25, member 27, mRNA (cDNA clone IMAGE:5006993), with apparent retained intron.
AB106930 - Mus musculus UCP4 mRNA for uncoupling protein 4, complete cds.
AK014394 - Mus musculus adult male brain cDNA, RIKEN full-length enriched library, clone:3632410G24 product:UNCOUPLING PROTEIN UCP-4, ISOFORM A homolog [Rattus norvegicus], full insert sequence.
BC138993 - Mus musculus solute carrier family 25, member 27, mRNA (cDNA clone MGC:170620 IMAGE:8862015), complete cds.
BC138994 - Mus musculus solute carrier family 25, member 27, mRNA (cDNA clone MGC:170621 IMAGE:8862016), complete cds.
AK043831 - Mus musculus 10 days neonate cortex cDNA, RIKEN full-length enriched library, clone:A830038H13 product:UNCOUPLING PROTEIN UCP-4, ISOFORM A homolog [Rattus norvegicus], full insert sequence.
AK219158 - Mus musculus cDNA, clone:Y2G0147I23, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000024705, based on BLAT search.
AK021314 - Mus musculus 13 days embryo stomach cDNA, RIKEN full-length enriched library, clone:D530043E16 product:similar to UNCOUPLING PROTEIN UCP-4, ISOFORM A [Rattus norvegicus], full insert sequence.
AK054292 - Mus musculus 2 days pregnant adult female ovary cDNA, RIKEN full-length enriched library, clone:E330010P20 product:weakly similar to MITOCHONDRIAL UNCOUPLING PROTEIN 4 (UCP 4) [Homo sapiens], full insert sequence.
BC099432 - Mus musculus cDNA clone IMAGE:30840315.
BC060279 - Mus musculus cDNA clone IMAGE:30146772, partial cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9D6D0 (Reactome details) participates in the following event(s):

R-MMU-166220 FA anion diffuses laterally to UCP
R-MMU-166387 The FA anion diffuses away laterally from UCP
R-MMU-166214 FA anion flip-flops to the opposite surface
R-MMU-170026 Protons are translocated from the intermembrane space to the matrix
R-MMU-167826 The fatty acid cycling model
R-MMU-166187 Mitochondrial Uncoupling Proteins
R-MMU-167827 The proton buffering model
R-MMU-163200 Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.
R-MMU-1428517 The citric acid (TCA) cycle and respiratory electron transport
R-MMU-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ENSMUST00000024705.1, ENSMUST00000024705.2, ENSMUST00000024705.3, ENSMUST00000024705.4, ENSMUST00000024705.5, mCG_13945, NM_028711, Q9D6D0, Q9D6D0_MOUSE, uc008cpk.1, uc008cpk.2, uc008cpk.3, UCP4
UCSC ID: ENSMUST00000024705.6
RefSeq Accession: NM_028711
Protein: Q9D6D0

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.