Gene interactions and pathways from curated databases and text-mining
Toxicol Lett 1999, PMID: 10079054

Acetylaminofluorene inhibits nitric oxide production in LPS-stimulated RAW 264.7 cells by blocking NF-kappa B/Rel activation.

Jeon, Y J; Han, S H; Kang, J S; Koh, W S; Yang, K H

The mechanism by which 2-acetylaminofluorene (AAF) inhibited nitric oxide (NO) formation, in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was investigated. The decrease in NO, as demonstrated by measurement of nitrite was found to correlate well with a decrease in inducible nitric oxide synthase (iNOS) mRNA. Since the promoter in iNOS gene contains binding motifs for NF-kappa B/Rel, AP-1, and NF-IL6 which appear to be important for LPS-mediated iNOS induction, the effect of AAF on the activation of these transcription factors was determined. Treatment of AAF to RAW 264.7 cells induced a dose-related inhibition of NF-kappa B/Rel in chloramphenicol acetyltransferase activity, while either AP-1 or NF-IL6 activation was not affected by AAF. Treatment of RAW 264.7 cells with AAF inhibited protein/DNA binding of NF-kappa B/Rel to its cognate site as measured by electrophoretic mobility shift assay. In addition, AAF treatment caused a significant reduction of nuclear c-rel, p65, and p50 protein levels, and this decrease was paralleled by the accumulation of cytoplasmic c-rel, p65, and p50. These data suggest that AAF inhibits iNOS gene expression by a mechanism involving a blockade of LPS-induced nuclear translocation of NF-kappa B/Rel.

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Text Mining Data

iNOS → LPS: " Since the promoter in iNOS gene contains binding motifs for NF-kappa B/Rel, AP-1, and NF-IL6 which appear to be important for LPS mediated iNOS induction, the effect of AAF on the activation of these transcription factors was determined "

NF-kappa B/Rel → AP-1: " Treatment of AAF to RAW 264.7 cells induced a dose related inhibition of NF-kappa B/Rel in chloramphenicol acetyltransferase activity, while either AP-1 or NF-IL6 activation was not affected by AAF "

NF-kappa B/Rel → NF-IL6: " Treatment of AAF to RAW 264.7 cells induced a dose related inhibition of NF-kappa B/Rel in chloramphenicol acetyltransferase activity, while either AP-1 or NF-IL6 activation was not affected by AAF "

B/Rel → AP-1: " Treatment of AAF to RAW 264.7 cells induced a dose related inhibition of NF-kappa B/Rel in chloramphenicol acetyltransferase activity, while either AP-1 or NF-IL6 activation was not affected by AAF "

B/Rel → NF-IL6: " Treatment of AAF to RAW 264.7 cells induced a dose related inhibition of NF-kappa B/Rel in chloramphenicol acetyltransferase activity, while either AP-1 or NF-IL6 activation was not affected by AAF "

NF-kappa B/Rel → LPS: " These data suggest that AAF inhibits iNOS gene expression by a mechanism involving a blockade of LPS induced nuclear translocation of NF-kappa B/Rel "

B/Rel → LPS: " These data suggest that AAF inhibits iNOS gene expression by a mechanism involving a blockade of LPS induced nuclear translocation of NF-kappa B/Rel "

Manually curated Databases

No curated data.