Brain Res Mol Brain Res 1999,
PMID: 10320796
Nemoto, K; Sekimoto, M; Fukamachi, K; Nemoto, F; Miyata, S; Nakamura, Y; Hamada, M; Senba, E; Ueyama, T; Degawa, M
We have previously reported that in cultured rat vascular smooth muscle cells (VSMCs), neurotrophin-3 (NT-3) gene expression was suppressed by TPA (12-O-tetradecanoyl phorbol-13-acetate), which induces an AP-1 transcription factor. In the present study, to clarify the mechanism for TPA-mediated downregulation of NT-3 gene expression, effects of cycloheximide and dexamethasone (Dex) on the TPA-mediated downregulation were examined in VSMCs. Pretreatment with cycloheximide, an inhibitor of protein synthesis, or simultaneous treatment with Dex, an inhibitor of AP-1, suppressed the TPA-mediated downregulation of NT-3 gene expression. Furthermore, co-transfection of c-fos and c-jun expression vectors into VSMCs resulted in decrease in the NT-3 gene expression. The present findings suggest that TPA-induced AP-1 de novo synthesis causes the downregulation of NT-3 gene expression in VSMCs.
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Text Mining Data
NT-3 ⊣ AP-1: "
The present findings suggest that TPA induced
AP-1 de novo synthesis
causes the downregulation of
NT-3 gene expression in VSMCs
"
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