Gene interactions and pathways from curated databases and text-mining
Genes Dev 2000, PMID: 10766741

Severe liver degeneration and lack of NF-kappaB activation in NEMO/IKKgamma-deficient mice.

Rudolph, D; Yeh, W C; Wakeham, A; Rudolph, B; Nallainathan, D; Potter, J; Elia, A J; Mak, T W

Phosphorylation of IkappaB, an inhibitor of NF-kappaB, is an important step in the activation of the transcription factor NF-kappaB. Phosphorylation is mediated by the IkappaB kinase (IKK) complex, known to contain two catalytic subunits: IKKalpha and IKKbeta. A novel, noncatalytic component of this kinase complex called NEMO (NF-kappaB essential modulator)/IKKgamma was identified recently. We have generated NEMO/IKKgamma-deficient mice by gene targeting. Mutant embryos die at E12.5-E13.0 from severe liver damage due to apoptosis. NEMO/IKKgamma-deficient primary murine embryonic fibroblasts (MEFs) lack detectable NF-kappaB DNA-binding activity in response to TNFalpha, IL-1, LPS, and Poly(IC) and do not show stimulus-dependent IkappaB kinase activity, which correlates with a lack of phosphorylation and degradation of IkappaBalpha. Consistent with these data, mutant MEFs show increased sensitivity to TNFalpha-induced apoptosis. Our data provide in vivo evidence that NEMO/IKKgamma is the first essential, noncatalytic component of the IKK complex.

Diseases/Pathways annotated by Medline MESH: Fetal Death
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Text Mining Data

(IKK) complex → IKKalpha: " Phosphorylation is mediated by the IkappaB kinase (IKK) complex , known to contain two catalytic subunits : IKKalpha and IKKbeta "

IkappaB kinase (IKK) → IKKbeta: " Phosphorylation is mediated by the IkappaB kinase (IKK) complex, known to contain two catalytic subunits : IKKalpha and IKKbeta "

(IKK) complex → IKKbeta: " Phosphorylation is mediated by the IkappaB kinase (IKK) complex , known to contain two catalytic subunits : IKKalpha and IKKbeta "

NF-kappaB → TNFalpha: " NEMO/IKKgamma-deficient primary murine embryonic fibroblasts ( MEFs ) lack detectable NF-kappaB DNA binding activity in response to TNFalpha , IL-1, LPS, and Poly ( IC ) and do not show stimulus dependent IkappaB kinase activity, which correlates with a lack of phosphorylation and degradation of IkappaBalpha "

NF-kappaB → IL-1: " NEMO/IKKgamma-deficient primary murine embryonic fibroblasts ( MEFs ) lack detectable NF-kappaB DNA binding activity in response to TNFalpha, IL-1 , LPS, and Poly ( IC ) and do not show stimulus dependent IkappaB kinase activity, which correlates with a lack of phosphorylation and degradation of IkappaBalpha "

NF-kappaB → LPS: " NEMO/IKKgamma-deficient primary murine embryonic fibroblasts ( MEFs ) lack detectable NF-kappaB DNA binding activity in response to TNFalpha, IL-1, LPS , and Poly ( IC ) and do not show stimulus dependent IkappaB kinase activity, which correlates with a lack of phosphorylation and degradation of IkappaBalpha "

Manually curated Databases

No curated data.