Gene interactions and pathways from curated databases and text-mining
J Biol Chem 2000, PMID: 11053425

CARD9 is a novel caspase recruitment domain-containing protein that interacts with BCL10/CLAP and activates NF-kappa B.

Bertin, J; Guo, Y; Wang, L; Srinivasula, S M; Jacobson, M D; Poyet, J L; Merriam, S; Du, M Q; Dyer, M J; Robison, K E; DiStefano, P S; Alnemri, E S

BCL10/CLAP is an activator of apoptosis and NF-kappaB signaling pathways and has been implicated in B cell lymphomas of mucosa-associated lymphoid tissue. Although its role in apoptosis remains to be determined, BCL10 likely activates NF-kappaB through the IKK complex in response to upstream stimuli. The N-terminal caspase recruitment domain (CARD) of BCL10 has been proposed to function as an activation domain that mediates homophilic interactions with an upstream CARD-containing NF-kappaB activator. To identify upstream signaling partners of BCL10, we performed a mammalian two-hybrid analysis and identified CARD9 as a novel CARD-containing protein that interacts selectively with the CARD activation domain of BCL10. When expressed in cells, CARD9 binds to BCL10 and activates NF-kappaB. Furthermore, endogenous CARD9 is found associated with BCL10 suggesting that both proteins form a pre-existing signaling complex within cells. CARD9 also self-associates and contains extensive coiled-coil motifs that may function as oligomerization domains. We propose here that CARD9 is an upstream activator of BCL10 and NF-kappaB signaling.

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Text Mining Data

NF-kappa B → CARD9: " CARD9 is a novel caspase recruitment domain containing protein that interacts with BCL10/CLAP and activates NF-kappa B "

NF-kappaB → BCL10/CLAP: " BCL10/CLAP is an activator of apoptosis and NF-kappaB signaling pathways and has been implicated in B cell lymphomas of mucosa associated lymphoid tissue "

NF-kappaB → BCL10: " Although its role in apoptosis remains to be determined, BCL10 likely activates NF-kappaB through the IKK complex in response to upstream stimuli "

NF-kappaB → BCL10: " Although its role in apoptosis remains to be determined, BCL10 likely activates NF-kappaB through the IKK complex in response to upstream stimuli "

NF-kappaB → CARD9: " When expressed in cells, CARD9 binds to BCL10 and activates NF-kappaB "

NF-kappaB → CARD9: " When expressed in cells, CARD9 binds to BCL10 and activates NF-kappaB "

NF-kappaB → CARD9: " We propose here that CARD9 is an upstream activator of BCL10 and NF-kappaB signaling "

BCL10 → CARD9: " We propose here that CARD9 is an upstream activator of BCL10 and NF-kappaB signaling "

Manually curated Databases

  • IRef Bind Interaction: CARD9 — CARD9
  • IRef Bind Interaction: BCL10 — CARD9
  • IRef Bind_translation Interaction: CARD9 — CARD9 (coimmunoprecipitation)
  • IRef Bind_translation Interaction: BCL10 — CARD9 (coimmunoprecipitation)
  • IRef Bind_translation Interaction: BCL10 — CARD9 (two hybrid)
  • IRef Bind_translation Interaction: BCL10 — CARD9 (imaging technique)
  • IRef Bind_translation Interaction: BCL10 — CARD9 (affinity chromatography technology)
  • IRef Biogrid Interaction: CARD9 — CARD9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BCL10 — CARD9 (direct interaction, two hybrid)
  • IRef Biogrid Interaction: BCL10 — CARD9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BCL10 — CARD9 (direct interaction, pull down)
  • IRef Hprd Interaction: CARD9 — CARD9 (in vivo)
  • IRef Hprd Interaction: BCL10 — CARD9 (in vivo)
  • IRef Hprd Interaction: BCL10 — CARD9 (in vitro)
  • IRef Innatedb Interaction: CARD9 — CARD9 (unknown, -)
  • IRef Innatedb Interaction: BCL10 — CARD9 (unknown, -)
In total, 2 gene pairs are associated to this article in curated databases