J Biol Chem 2000,
PMID: 11053425
Bertin, J; Guo, Y; Wang, L; Srinivasula, S M; Jacobson, M D; Poyet, J L; Merriam, S; Du, M Q; Dyer, M J; Robison, K E; DiStefano, P S; Alnemri, E S
BCL10/CLAP is an activator of apoptosis and NF-kappaB signaling pathways and has been implicated in B cell lymphomas of mucosa-associated lymphoid tissue. Although its role in apoptosis remains to be determined, BCL10 likely activates NF-kappaB through the IKK complex in response to upstream stimuli. The N-terminal caspase recruitment domain (CARD) of BCL10 has been proposed to function as an activation domain that mediates homophilic interactions with an upstream CARD-containing NF-kappaB activator. To identify upstream signaling partners of BCL10, we performed a mammalian two-hybrid analysis and identified CARD9 as a novel CARD-containing protein that interacts selectively with the CARD activation domain of BCL10. When expressed in cells, CARD9 binds to BCL10 and activates NF-kappaB. Furthermore, endogenous CARD9 is found associated with BCL10 suggesting that both proteins form a pre-existing signaling complex within cells. CARD9 also self-associates and contains extensive coiled-coil motifs that may function as oligomerization domains. We propose here that CARD9 is an upstream activator of BCL10 and NF-kappaB signaling.
Document information provided by NCBI PubMed
Text Mining Data
NF-kappa B → CARD9: "
CARD9 is a novel caspase recruitment domain containing protein that interacts with BCL10/CLAP and
activates NF-kappa B
"
NF-kappaB → BCL10/CLAP: "
BCL10/CLAP is an activator of apoptosis and NF-kappaB signaling pathways and has been implicated in B cell lymphomas of mucosa associated lymphoid tissue
"
NF-kappaB → BCL10: "
Although its role in apoptosis remains to be determined, BCL10 likely activates NF-kappaB through the IKK complex in response to upstream stimuli
"
NF-kappaB → BCL10: "
Although its role in apoptosis remains to be determined, BCL10 likely activates NF-kappaB through the IKK complex in response to upstream stimuli
"
NF-kappaB → CARD9: "
When expressed in cells, CARD9 binds to BCL10 and activates NF-kappaB
"
NF-kappaB → CARD9: "
When expressed in cells, CARD9 binds to BCL10 and activates NF-kappaB
"
NF-kappaB → CARD9: "
We propose here that CARD9 is an upstream activator of BCL10 and NF-kappaB signaling
"
BCL10 → CARD9: "
We propose here that CARD9 is an upstream activator of BCL10 and NF-kappaB signaling
"
Manually curated Databases
-
IRef Bind Interaction:
CARD9
—
CARD9
-
IRef Bind Interaction:
BCL10
—
CARD9
-
IRef Bind_translation Interaction:
CARD9
—
CARD9
(coimmunoprecipitation)
-
IRef Bind_translation Interaction:
BCL10
—
CARD9
(coimmunoprecipitation)
-
IRef Bind_translation Interaction:
BCL10
—
CARD9
(two hybrid)
-
IRef Bind_translation Interaction:
BCL10
—
CARD9
(imaging technique)
-
IRef Bind_translation Interaction:
BCL10
—
CARD9
(affinity chromatography technology)
-
IRef Biogrid Interaction:
CARD9
—
CARD9
(physical association, affinity chromatography technology)
-
IRef Biogrid Interaction:
BCL10
—
CARD9
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
BCL10
—
CARD9
(physical association, affinity chromatography technology)
-
IRef Biogrid Interaction:
BCL10
—
CARD9
(direct interaction, pull down)
-
IRef Hprd Interaction:
CARD9
—
CARD9
(in vivo)
-
IRef Hprd Interaction:
BCL10
—
CARD9
(in vivo)
-
IRef Hprd Interaction:
BCL10
—
CARD9
(in vitro)
-
IRef Innatedb Interaction:
CARD9
—
CARD9
(unknown, -)
-
IRef Innatedb Interaction:
BCL10
—
CARD9
(unknown, -)
In total, 2 gene pairs are associated to this article in curated databases