Gene interactions and pathways from curated databases and text-mining
J Am Soc Nephrol 2001, PMID: 11274229

Suppression of constitutive but not Il-1beta-inducible expression of monocyte chemoattractant protein-1 in mesangial cells by retinoic acids: intervention in the activator protein-1 pathway.

Lucio-Cazana, J; Nakayama, K; Xu, Q; Konta, T; Moreno-Manzano, V; Furusu, A; Kitamura, M

Retinoic acid regulates a wide range of biologic processes, including inflammation. This study investigated the effect of all-trans-retinoic acid (t-RA) on the constitutive and cytokine-inducible expression of monocyte chemoattractant protein 1 (MCP-1) in rat mesangial cells. Serum-deprived mesangial cells exhibited substantial levels of MCP-1 mRNA, and the expression was markedly upregulated by interleukin-1beta (IL-1beta). Pretreatment with t-RA abrogated the constitutive mRNA expression but did not inhibit the IL-1beta-inducible expression. The similar effects were observed by 9-cis-RA. The suppressive effect of t-RA required retinoic acid receptors. t-RA did not affect the stability of MCP-1 mRNA, indicating that its suppressive effect was at the transcriptional level. Experiments that used pharmacologic and genetic inhibitors showed that the IL-1beta-inducible MCP-1 expression was dependent on nuclear factor-kappaB (NF-kappaB) and independent of activator protein 1 (AP-1). In contrast, the constitutive expression of MCP-1 was dependent on both NF-kappaB and AP-1. t-RA substantially inhibited the constitutive activity of AP-1 but did not inhibit NF-kappaB activity in mesangial cells. These data suggested that (1) constitutive and IL-1beta-inducible expression of MCP-1 was differently regulated by AP-1 and NF-kappaB and (2) t-RA inhibited selectively the constitutive expression of MCP-1 via intervention in the AP-1 pathway.

Document information provided by NCBI PubMed

Text Mining Data

MCP-1 → nuclear factor-kappaB (NF-kappaB): " Experiments that used pharmacologic and genetic inhibitors showed that the IL-1beta-inducible MCP-1 expression was dependent on nuclear factor-kappaB (NF-kappaB) and independent of activator protein 1 (AP-1) "

IL-1beta-inducible → nuclear factor-kappaB (NF-kappaB): " Experiments that used pharmacologic and genetic inhibitors showed that the IL-1beta-inducible MCP-1 expression was dependent on nuclear factor-kappaB (NF-kappaB) and independent of activator protein 1 (AP-1) "

MCP-1 — activator protein 1 (AP-1): " Experiments that used pharmacologic and genetic inhibitors showed that the IL-1beta-inducible MCP-1 expression was dependent on nuclear factor-kappaB (NF-kappaB) and independent of activator protein 1 (AP-1) "

IL-1beta-inducible — activator protein 1 (AP-1): " Experiments that used pharmacologic and genetic inhibitors showed that the IL-1beta-inducible MCP-1 expression was dependent on nuclear factor-kappaB (NF-kappaB) and independent of activator protein 1 (AP-1) "

MCP-1 → AP-1: " In contrast, the constitutive expression of MCP-1 was dependent on both NF-kappaB and AP-1 "

MCP-1 → NF-kappaB: " In contrast, the constitutive expression of MCP-1 was dependent on both NF-kappaB and AP-1 "

AP-1 ⊣ NF-kappaB: " t-RA substantially inhibited the constitutive activity of AP-1 but did not inhibit NF-kappaB activity in mesangial cells "

MCP-1 ⊣ AP-1: " These data suggested that ( 1 ) constitutive and IL-1beta-inducible expression of MCP-1 was differently regulated by AP-1 and NF-kappaB and ( 2 ) t-RA inhibited selectively the constitutive expression of MCP-1 via intervention in the AP-1 pathway "

MCP-1 ⊣ IL-1beta-inducible: " These data suggested that ( 1 ) constitutive and IL-1beta-inducible expression of MCP-1 was differently regulated by AP-1 and NF-kappaB and ( 2 ) t-RA inhibited selectively the constitutive expression of MCP-1 via intervention in the AP-1 pathway "

MCP-1 ⊣ NF-kappaB: " These data suggested that ( 1 ) constitutive and IL-1beta-inducible expression of MCP-1 was differently regulated by AP-1 and NF-kappaB and ( 2 ) t-RA inhibited selectively the constitutive expression of MCP-1 via intervention in the AP-1 pathway "

IL-1beta-inducible → AP-1: " These data suggested that ( 1 ) constitutive and IL-1beta-inducible expression of MCP-1 was differently regulated by AP-1 and NF-kappaB and ( 2 ) t-RA inhibited selectively the constitutive expression of MCP-1 via intervention in the AP-1 pathway "

IL-1beta-inducible → NF-kappaB: " These data suggested that ( 1 ) constitutive and IL-1beta-inducible expression of MCP-1 was differently regulated by AP-1 and NF-kappaB and ( 2 ) t-RA inhibited selectively the constitutive expression of MCP-1 via intervention in the AP-1 pathway "

Manually curated Databases

No curated data.