FEBS Lett 2005,
PMID: 16107255
Son, Dong Ok; Satsu, Hideo; Shimizu, Makoto
We investigated the effect of several amino acids on the secretion of such inflammatory cytokines as interleukin-8 (IL-8) induced by hydrogen peroxide or tumor necrosis factor-alpha (TNF-alpha) in intestinal epithelial-like Caco-2 and HT-29 cells. We found that histidine, one of the conditionally essential amino acids, significantly inhibited both hydrogen peroxide- and TNF-alpha-induced IL-8 secretion and mRNA expression in Caco-2 cells and HT-29 cells. These inhibitions were dose dependent and the inhibition rate of hydrogen peroxide-induced IL-8 secretion reached more than 50% at a concentration of 25mM, with over 95% inhibition at a concentration of 50mM. TNF-alpha increased the transcriptional activity of the IL-8 promoter which was significantly inhibited by treating Caco-2 cells with histidine. Histidine also abolished the NF-kappaB-dependent activation of the IL-8 promoter induced by TNF-alpha. These results indicate that histidine inhibited the hydrogen peroxide- and TNF-alpha-induced IL-8 secretion at the transcriptional level in intestinal epithelial cells, suggesting that histidine has the potential to attenuate intestinal inflammation.
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Text Mining Data
interleukin-8 → TNF-alpha: "
Histidine inhibits oxidative stress- and
TNF-alpha induced
interleukin-8 secretion in intestinal epithelial cells
"
interleukin-8 (IL-8) → tumor necrosis factor-alpha (TNF-alpha): "
We investigated the effect of several amino acids on the secretion of such inflammatory cytokines as interleukin-8 (IL-8) induced by hydrogen peroxide or tumor necrosis factor-alpha (TNF-alpha) in intestinal epithelial-like Caco-2 and HT-29 cells
"
IL-8 → TNF-alpha: "
We found that histidine, one of the conditionally essential amino acids, significantly inhibited both hydrogen peroxide- and TNF-alpha induced IL-8 secretion and mRNA expression in Caco-2 cells and HT-29 cells
"
IL-8 → TNF-alpha: "
TNF-alpha increased the transcriptional activity of the IL-8 promoter which was significantly inhibited by treating Caco-2 cells with histidine
"
IL-8 → NF-kappaB: "
Histidine also abolished the NF-kappaB dependent activation of the IL-8 promoter induced by TNF-alpha
"
IL-8 → TNF-alpha: "
These results indicate that histidine inhibited the hydrogen peroxide- and TNF-alpha induced IL-8 secretion at the transcriptional level in intestinal epithelial cells, suggesting that histidine has the potential to attenuate intestinal inflammation
"
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