Gene interactions and pathways from curated databases and text-mining
Int Immunol 2005, PMID: 16263754

CpG oligodeoxynucleotides induce IL-8 expression in CD34+ cells via mitogen-activated protein kinase-dependent and NF-kappaB-independent pathways.

Kim, Jung Mogg; Kim, Nam In; Oh, Yu-Kyoung; Kim, Young-Jeon; Youn, Jeehee; Ahn, Myung-Ju

To elucidate the role of Toll-like receptor 9 (TLR9) activation along with the intracellular signaling pathways triggered by CpG DNA in CD34+ cells, we investigated whether synthetic oligodeoxynucleotides (ODNs), containing unmethylated CpG motifs, could induce IL-8 expression in CD34+ cells through mitogen-activated protein kinase (MAPK) or nuclear factor-kappaB (NF-kappaB) pathway. We demonstrated evidence for the first time that CD34+ cells constitutively expressed TLR9. Exposure of the cells to CpG ODN resulted in a time- and dose-dependent increase of IL-8 expression, and activation of phosphorylated ERK1/2 and phosphorylated p38. In addition, CpG ODN stimulated AP-1, but not NF-kappaB, signals. Moreover, inhibitors of MAPK (U0126 and SB203580) significantly reduced the IL-8 production, while the inhibition of NF-kappaB (pyrrolidinedithiocarbamate and retrovirus containing dominant-negative IkappaB alpha plasmid) did not affect the IL-8 expression increased by CpG ODN. Moreover, co-stimulation with LPS and CpG synergistically up-regulates IL-8 in CD34+ cells. These results suggest that CpG DNA, acting on TLR9, activates CD34+ cells to express IL-8 through MAPK-dependent and NF-kappaB-independent pathways.

Diseases/Pathways annotated by Medline MESH: MAP Kinase Signaling System
Document information provided by NCBI PubMed

Text Mining Data

mitogen activated protein kinase → IL-8: " To elucidate the role of Toll-like receptor 9 (TLR9) activation along with the intracellular signaling pathways triggered by CpG DNA in CD34+ cells, we investigated whether synthetic oligodeoxynucleotides ( ODNs ), containing unmethylated CpG motifs, could induce IL-8 expression in CD34+ cells through mitogen activated protein kinase ( MAPK ) or nuclear factor-kappaB (NF-kappaB) pathway "

p38 → IL-8: " Exposure of the cells to CpG ODN resulted in a time- and dose dependent increase of IL-8 expression, and activation of phosphorylated ERK1/2 and phosphorylated p38 "

ERK1/2 → IL-8: " Exposure of the cells to CpG ODN resulted in a time- and dose dependent increase of IL-8 expression, and activation of phosphorylated ERK1/2 and phosphorylated p38 "

IL-8 ⊣ MAPK: " Moreover, inhibitors of MAPK ( U0126 and SB203580 ) significantly reduced the IL-8 production, while the inhibition of NF-kappaB ( pyrrolidinedithiocarbamate and retrovirus containing dominant negative IkappaB alpha plasmid ) did not affect the IL-8 expression increased by CpG ODN "

NF-kappaB ⊣ MAPK: " Moreover, inhibitors of MAPK ( U0126 and SB203580 ) significantly reduced the IL-8 production, while the inhibition of NF-kappaB ( pyrrolidinedithiocarbamate and retrovirus containing dominant negative IkappaB alpha plasmid ) did not affect the IL-8 expression increased by CpG ODN "

IL-8 — NF-kappaB: " Moreover, inhibitors of MAPK ( U0126 and SB203580 ) significantly reduced the IL-8 production, while the inhibition of NF-kappaB ( pyrrolidinedithiocarbamate and retrovirus containing dominant negative IkappaB alpha plasmid ) did not affect the IL-8 expression increased by CpG ODN "

Manually curated Databases

No curated data.