Gene interactions and pathways from curated databases and text-mining
Mol Immunol 2007, PMID: 16806476

Saxatilin inhibits TNF-alpha-induced proliferation by suppressing AP-1-dependent IL-8 expression in the ovarian cancer cell line MDAH 2774.

Kim, Dong Seok; Jang, Yoon-Jung; Jeon, Ok-Hee; Kim, Doo-Sik

Tumor necrosis factor-alpha (TNF-alpha)-induced proliferation of cancer cell line MDAH 2774 was significantly suppressed by treating the cells with saxatilin, a snake venom disintegrin. The suppressed proliferation was found to be associated with the level of interleukin-8 (IL-8) expression in the cells. TNF-alpha-induced IL-8 promoter activation that is inhibited by saxatilin treatment was dependent on activating protein-1 (AP-1) instead of nuclear factor-kappa B (NF-kappaB). Coexpression of dominant negative p38 (DN-p38) suggested that p38 is involved in the IL-8 promoter activity which is regulated by saxatilin or TNF-alpha. Experimental evidence clearly indicated that saxatilin inhibits TNF-alpha-induced proliferation of the ovarian cancer cells by suppressing IL-8 expression in AP-1-dependent manner.

Diseases/Pathways annotated by Medline MESH: Ovarian Neoplasms
Document information provided by NCBI PubMed

Text Mining Data

IL-8 → AP-1: " Saxatilin inhibits TNF-alpha induced proliferation by suppressing AP-1 dependent IL-8 expression in the ovarian cancer cell line MDAH 2774 "

IL-8 → TNF-alpha: " TNF-alpha induced IL-8 promoter activation that is inhibited by saxatilin treatment was dependent on activating protein-1 (AP-1) instead of nuclear factor-kappa B (NF-kappaB) "

IL-8 → activating protein-1 (AP-1): " TNF-alpha induced IL-8 promoter activation that is inhibited by saxatilin treatment was dependent on activating protein-1 (AP-1) instead of nuclear factor-kappa B (NF-kappaB) "

IL-8 → nuclear factor-kappa B (NF-kappaB): " TNF-alpha induced IL-8 promoter activation that is inhibited by saxatilin treatment was dependent on activating protein-1 (AP-1) instead of nuclear factor-kappa B (NF-kappaB) "

IL-8 → p38: " Coexpression of dominant negative p38 ( DN-p38 ) suggested that p38 is involved in the IL-8 promoter activity which is regulated by saxatilin or TNF-alpha "

IL-8 — AP-1: " Experimental evidence clearly indicated that saxatilin inhibits TNF-alpha induced proliferation of the ovarian cancer cells by suppressing IL-8 expression in AP-1 dependent manner "

Manually curated Databases

No curated data.