Gene interactions and pathways from curated databases and text-mining
J Hypertens 2008, PMID: 18300858

The deleterious effect of high concentrations of D-glucose requires pro-inflammatory preconditioning.

Lafuente, Nuria; Matesanz, Nuria; Azcutia, Verónica; Romacho, Tania; Nevado, Julián; Rodríguez-Mañas, Leocadio; Moncada, Salvador; Peiró, Concepción; Sánchez-Ferrer, Carlos F

OBJECTIVE

The present study investigated whether high concentrations of D-glucose can trigger pro-inflammatory mechanisms in human aortic smooth muscle cells.

METHODS

The expression and/or the activity of inducible nitric oxide synthase (iNOS), the extracellular signal-regulated kinase (ERK) 1/2 and nuclear factor (NF)-kappaB were studied in cultured human aortic smooth muscle cells (HASMC) in response to increasing concentrations of D-glucose and/or the inflammatory cytokine interleukin (IL)-1beta.

RESULTS

Increasing D-glucose in the medium from 5.5 to 22 mmol/l had no effect on any of these parameters. However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta (2.5 and 5 ng/ml), as well as the IL-1beta-induced activation of both ERK 1/2 and NF-kappaB. D-glucose also enhanced, concentration-dependently, the expression and activity of iNOS induced by co-incubation with IL-1beta (10 ng/ml). Pretreatment with IL-1beta sensitized the cells to the subsequent effects of high D-glucose.

CONCLUSIONS

The results indicate that high concentrations of D-glucose exacerbate the pro-inflammatory effects of IL-1beta. We suggest that the observed association between inflammation and diabetes is the result of elevated D-glucose enhancing a pre-existing inflammatory condition, rather than a direct effect of D-glucose on the production of inflammatory mediators.

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Text Mining Data

IL-1beta → ERK 1/2: " However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta ( 2.5 and 5 ng/ml ), as well as the IL-1beta induced activation of both ERK 1/2 and NF-kappaB "

IL-1beta → ERK 1/2: " However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta ( 2.5 and 5 ng/ml ), as well as the IL-1beta induced activation of both ERK 1/2 and NF-kappaB "

IL-1beta → NF-kappaB: " However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta ( 2.5 and 5 ng/ml ), as well as the IL-1beta induced activation of both ERK 1/2 and NF-kappaB "

iNOS → IL-1beta: " However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta ( 2.5 and 5 ng/ml ), as well as the IL-1beta induced activation of both ERK 1/2 and NF-kappaB "

iNOS → ERK 1/2: " However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta ( 2.5 and 5 ng/ml ), as well as the IL-1beta induced activation of both ERK 1/2 and NF-kappaB "

iNOS → ERK 1/2: " However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta ( 2.5 and 5 ng/ml ), as well as the IL-1beta induced activation of both ERK 1/2 and NF-kappaB "

iNOS → NF-kappaB: " However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta ( 2.5 and 5 ng/ml ), as well as the IL-1beta induced activation of both ERK 1/2 and NF-kappaB "

iNOS → IL-1beta: " D-glucose also enhanced, concentration-dependently, the expression and activity of iNOS induced by co-incubation with IL-1beta ( 10 ng/ml ) "

Manually curated Databases

No curated data.