Gene interactions and pathways from curated databases and text-mining
Obesity (Silver Spring) 2008, PMID: 18388891

High-fat diet alters PP2A, TC10, and CIP4 expression in visceral adipose tissue of rats.

Jun, Hye-Seung; Hwang, Kyujung; Kim, Yunjung; Park, Taesun

OBJECTIVE

The aim of this study was to investigate a possible link between high-fat diet (HFD)-induced obesity and the expression of protein phosphatase 2A (PP2A) and Cdc42-interacting protein 4 (CIP4) proteins, potential downstream components of the IRS/PI3K/AKT and CAP/Cbl/TC10 pathway, respectively, in the visceral adipose tissue.

METHODS

Twenty male Sprague-Dawley rats were randomly divided into two groups and were given either HFD or the normal diet (ND) for 8 weeks. The HFD-induced changes in the expression of the epididymal adipose tissue genes involved in the insulin-signaling pathways were evaluated using real-time reverse-transcription PCR and western blot analysis.

RESULTS

The exposure of rats to HFD for 8 weeks resulted in a significant increase in the expression of PP2A at both the transcriptional and translational levels, along with a marked reduction in the levels of phosphorylated AKT and insulin receptor substrate-1 (IRS-1) in the cytosol of visceral adipocytes, compared with the ND rats. Besides, there were significant HFD-induced decreases in the mRNA and protein levels of CIP4 and TC10 in the adipose tissue of rats.

CONCLUSIONS

These data suggest that HFD might have a relevance to insulin resistance by increasing the expression of PP2A, an inhibitor of AKT activity in the phosphatidylinositol 3-kinase (PI3K)/AKT pathway, and also by suppressing the expression of TC10 and CIP4, downstream effectors of the Cbl/CAP/TC10 insulin-signaling cascade in the visceral adipose tissue.

Diseases/Pathways annotated by Medline MESH: Disease Models, Animal, Insulin Resistance, Obesity
Document information provided by NCBI PubMed

Text Mining Data

AKT ⊣ PP2A: " These data suggest that HFD might have a relevance to insulin resistance by increasing the expression of PP2A , an inhibitor of AKT activity in the phosphatidylinositol 3-kinase (PI3K)/AKT pathway, and also by suppressing the expression of TC10 and CIP4, downstream effectors of the Cbl/CAP/TC10 insulin signaling cascade in the visceral adipose tissue "

Manually curated Databases

No curated data.