Gene interactions and pathways from curated databases and text-mining
J Neuroimmune Pharmacol 2009, PMID: 19214751

WIN55,212-2 inhibits production of CX3CL1 by human astrocytes: involvement of p38 MAP kinase.

Sheng, W S; Hu, S; Ni, H T; Rock, R B; Peterson, P K

CX3CL1 (fractalkine) has been shown not only to be neuroprotective but also may play a role in HIV-1-associated neuropathogenesis. In this study, we found that production of CX3CL1 by human astrocytes stimulated with interleukin (IL)-1beta was inhibited in a concentration-dependent manner following pretreatment with the synthetic cannabinoid WIN55,212-2. The CB(2) receptor selective antagonist SR144528 significantly inhibited WIN55,212-2-mediated suppression of CX3CL1, suggesting a CB(2)-receptor-related mechanism. IL-1beta triggered the activation of p38 and ERK1/2 (p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation. This finding was mirrored in experiments using known inhibitors of these MAPKs, suggesting that the suppression of CX3CL1 production by WIN55,212-2 involves inhibition of signaling via p38 MAPK. Our results support the concept that synthetic cannabinoids have anti-inflammatory properties and that these agents may have therapeutic potential for certain neuroinflammatory disorders.

Diseases/Pathways annotated by Medline MESH: MAP Kinase Signaling System
Document information provided by NCBI PubMed

Text Mining Data

p44/42) ⊣ p38: " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

p44/42) ⊣ MAPK: " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

p38 ⊣ p38: " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

p38 ⊣ MAPK: " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

p38 ⊣ IL-1beta: " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

p38 ⊣ p44/42) MAP kinase (MAPK): " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

MAPK ⊣ IL-1beta: " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

MAPK ⊣ p44/42) MAP kinase (MAPK): " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

p44/42) → IL-1beta: " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

p38 → IL-1beta: " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

p44/42) MAP kinase (MAPK) → IL-1beta: " IL-1beta triggered the activation of p38 and ERK1/2 ( p44/42) MAP kinase (MAPK) signaling pathways, but WIN55,212-2 mainly inhibited p38 MAPK phosphorylation "

Manually curated Databases

No curated data.