Gene interactions and pathways from curated databases and text-mining
J Biol Chem 2009, PMID: 19258318

ULK1.ATG13.FIP200 complex mediates mTOR signaling and is essential for autophagy.

Ganley, Ian G; Lam, Du H; Wang, Junru; Ding, Xiaojun; Chen, She; Jiang, Xuejun

Autophagy is a degradative process that recycles long-lived and faulty cellular components. It is linked to many diseases and is required for normal development. ULK1, a mammalian serine/threonine protein kinase, plays a key role in the initial stages of autophagy, though the exact molecular mechanism is unknown. Here we report identification of a novel protein complex containing ULK1 and two additional protein factors, FIP200 and ATG13, all of which are essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. Additionally, we demonstrate by using both cellular experiments and a de novo in vitro reconstituted reaction that FIP200 and ATG13 can enhance ULK1 kinase activity individually but both are required for maximal stimulation. Further, we show that ATG13 and ULK1 are phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner, indicating that the ULK1.ATG13.FIP200 complex acts as a node for integrating incoming autophagy signals into autophagosome biogenesis.

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Text Mining Data

ULK1 → FIP200: " Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein "

ULK1 → ATG13: " Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein "

ULK1 → FIP200: " Additionally, we demonstrate by using both cellular experiments and a de novo in vitro reconstituted reaction that FIP200 and ATG13 can enhance ULK1 kinase activity individually but both are required for maximal stimulation "

ULK1 → ATG13: " Additionally, we demonstrate by using both cellular experiments and a de novo in vitro reconstituted reaction that FIP200 and ATG13 can enhance ULK1 kinase activity individually but both are required for maximal stimulation "

Manually curated Databases

  • NCI Pathway Database mTOR signaling pathway: mTORC1 complex (MTOR-MLST8-RPTOR) → mTORC1/ULK1-2/ATG13/FIP200 complex (MTOR-MLST8-RPTOR-ULK1_ULK2-ATG13-RB1CC1) (modification, collaborate)
    Evidence: mutant phenotype, assay, physical interaction
  • NCI Pathway Database mTOR signaling pathway: mTORC1 complex (MTOR-MLST8-RPTOR) → ULK1-2/ATG13/FIP200 complex (ULK1_ULK2-ATG13-RB1CC1) (modification, collaborate)
    Evidence: mutant phenotype, assay, physical interaction
  • NCI Pathway Database mTOR signaling pathway: mTORC1/ULK1-2/ATG13/FIP200 complex (MTOR-MLST8-RPTOR-ULK1_ULK2-ATG13-RB1CC1) → ULK1-2/ATG13/FIP200 complex (ULK1_ULK2-ATG13-RB1CC1) (modification, collaborate)
    Evidence: mutant phenotype, assay, physical interaction
In total, 28 gene pairs are associated to this article in curated databases