Gene interactions and pathways from curated databases and text-mining
J Infect Dis 1990, PMID: 2113076

Interleukin-1 induces tumor necrosis factor (TNF) in human peripheral blood mononuclear cells in vitro and a circulating TNF-like activity in rabbits.

Ikejima, T; Okusawa, S; Ghezzi, P; van der Meer, J W; Dinarello, C A

Recombinant human interleukin-1 (IL-1), injected into rabbits, induces the synthesis of endogenous IL-1. Also, IL-1 induces its own gene expression and synthesis in human peripheral blood mononuclear cells (PBMC). In this study, tumor necrosis factor-alpha (TNF-alpha) production by PBMC of 40 individuals stimulated with IL-1 alpha or IL-1 beta was determined by specific radioimmunoassay (RIA). After 3 h of PBMC incubation with IL-1, TNF-alpha mRNA was detected. IL-1 alpha stimulated both IL-1 beta and TNF-alpha, but there was no correlation in the amount of TNF-alpha or IL-1 beta synthesized in the PBMC of 29 individuals. IL-1-stimulated adherent cells produced approximately 50% more TNF-alpha than did unfractionated PBMC. Coincubation with interferon-gamma (IFN-gamma) did not change the amount of IL-1-induced TNF-alpha, whereas in the same culture IFN-gamma inhibited (greater than 70%) IL-1-induced IL-1 production. Endogenous pyrogen and TNF-like activity were detected in the sera of rabbits 3.5 h after injection of either IL-1 alpha or -1 beta. These studies demonstrate that IL-1 induced TNF-alpha production in vivo and in vitro.

Document information provided by NCBI PubMed

Text Mining Data

TNF-alpha → IL-1 alpha: " IL-1 alpha stimulated both IL-1 beta and TNF-alpha , but there was no correlation in the amount of TNF-alpha or IL-1 beta synthesized in the PBMC of 29 individuals "

IL-1 beta → IL-1 alpha: " IL-1 alpha stimulated both IL-1 beta and TNF-alpha, but there was no correlation in the amount of TNF-alpha or IL-1 beta synthesized in the PBMC of 29 individuals "

TNF-alpha → IL-1: " Coincubation with interferon-gamma (IFN-gamma) did not change the amount of IL-1 induced TNF-alpha , whereas in the same culture IFN-gamma inhibited ( greater than 70 % ) "

TNF-alpha → IL-1: " These studies demonstrate that IL-1 induced TNF-alpha production in vivo and in vitro "

Manually curated Databases

No curated data.