J Infect Dis 1990,
PMID: 2113076
Ikejima, T; Okusawa, S; Ghezzi, P; van der Meer, J W; Dinarello, C A
Recombinant human interleukin-1 (IL-1), injected into rabbits, induces the synthesis of endogenous IL-1. Also, IL-1 induces its own gene expression and synthesis in human peripheral blood mononuclear cells (PBMC). In this study, tumor necrosis factor-alpha (TNF-alpha) production by PBMC of 40 individuals stimulated with IL-1 alpha or IL-1 beta was determined by specific radioimmunoassay (RIA). After 3 h of PBMC incubation with IL-1, TNF-alpha mRNA was detected. IL-1 alpha stimulated both IL-1 beta and TNF-alpha, but there was no correlation in the amount of TNF-alpha or IL-1 beta synthesized in the PBMC of 29 individuals. IL-1-stimulated adherent cells produced approximately 50% more TNF-alpha than did unfractionated PBMC. Coincubation with interferon-gamma (IFN-gamma) did not change the amount of IL-1-induced TNF-alpha, whereas in the same culture IFN-gamma inhibited (greater than 70%) IL-1-induced IL-1 production. Endogenous pyrogen and TNF-like activity were detected in the sera of rabbits 3.5 h after injection of either IL-1 alpha or -1 beta. These studies demonstrate that IL-1 induced TNF-alpha production in vivo and in vitro.
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Text Mining Data
TNF-alpha → IL-1 alpha: "
IL-1 alpha stimulated both IL-1 beta and
TNF-alpha , but there was no correlation in the amount of TNF-alpha or IL-1 beta synthesized in the PBMC of 29 individuals
"
IL-1 beta → IL-1 alpha: "
IL-1 alpha stimulated both IL-1 beta and TNF-alpha, but there was no correlation in the amount of TNF-alpha or IL-1 beta synthesized in the PBMC of 29 individuals
"
TNF-alpha → IL-1: "
Coincubation with interferon-gamma (IFN-gamma) did not change the amount of IL-1 induced TNF-alpha , whereas in the same culture IFN-gamma inhibited ( greater than 70 % )
"
TNF-alpha → IL-1: "
These studies demonstrate that IL-1 induced TNF-alpha production in vivo and in vitro
"
Manually curated Databases
No curated data.