Lymphokine Cytokine Res 1994,
PMID: 7532017
Chaturvedi, M M; Higuchi, M; Aggarwal, B B
The nuclear transcription factor NF-kappa B has been identified as a critical component in signal transduction pathways. We used an electrophoretic gel mobility shift assay to examine the activation of NF-kappa B in human U-937 cells treated with tumor necrosis factor (TNF), lymphotoxin (LT), interferons (IFN)-alpha, IFN-beta, and IFN-gamma, interleukins (IL)-1 beta, IL-4, and IL-6, leukemia inhibitory factor (LIF), basic fibroblast growth factor (FGF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and transforming growth factor-beta (TGF-beta). Only TNF, LT, and IL-1 activated NF-kappa B. Since interferons have been shown to induce TNF receptors and potentiate TNF-mediated cellular responses, we also measured the effect of interferons on TNF-induced activation of NF-kappa B. Under our conditions, all three IFNs potentiated the cytotoxic effects of TNF but had no effect on the TNF-dependent NF-kappa B activation. These results suggest overall that the activation of NF-kappa B is not a generalized mediator of signal transduction of most cytokines and also that NF-kappa B activation is not sufficient for antiproliferative effects mediated through certain cytokines.
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Text Mining Data
NF-kappa B → TNF: "
Only
TNF , LT, and IL-1
activated NF-kappa B
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NF-kappa B → IL-1: "
Only TNF, LT, and IL-1 activated NF-kappa B
"
NF-kappa B → TNF: "
Since interferons have been shown to induce TNF receptors and potentiate TNF mediated cellular responses, we also measured the effect of interferons on TNF induced activation of NF-kappa B
"
NF-kappa B → TNF: "
Under our conditions, all three IFNs potentiated the cytotoxic effects of TNF but had no effect on the TNF dependent NF-kappa B activation
"
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