FEBS Lett 1995,
PMID: 7540991
Masamune, A; Hakomori, S; Igarashi, Y
We examined the effect of N,N,N-trimethylsphingosine (TMS) on the interleukin-1 beta (IL-1 beta)-induced E-selectin expression in human umbilical vein endothelial cells (HUVEC). Incubation of HUVEC with TMS (0.1-10 microM) resulted in a concentration-dependent inhibition of IL-1 beta-induced E-selectin expression. Sphingosine or N,N-dimethylsphingosine had no effects on the expression. Electrophoretic mobility shift assay revealed that TMS inhibited IL-1 beta-induced NF-kappa B activation, which is essential for E-selectin expression. This inhibitory effect of TMS on IL-1 beta-dependent endothelial cell activation may partly explain the known anti-inflammatory or anti-metastatic effect of TMS in vivo.
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Text Mining Data
E-selectin → interleukin-1 beta: "
N, N,N-trimethylsphingosine inhibits
interleukin-1 beta induced NF-kappa B activation and consequent
E-selectin expression in human umbilical vein endothelial cells
"
NF-kappa B → interleukin-1 beta: "
N, N,N-trimethylsphingosine inhibits interleukin-1 beta induced NF-kappa B activation and consequent E-selectin expression in human umbilical vein endothelial cells
"
E-selectin → interleukin-1 beta (IL-1 beta): "
We examined the effect of N, N,N-trimethylsphingosine ( TMS ) on the interleukin-1 beta (IL-1 beta) induced E-selectin expression in human umbilical vein endothelial cells ( HUVEC )
"
E-selectin → IL-1 beta: "
Incubation of HUVEC with TMS ( 0.1-10 microM ) resulted in a concentration dependent inhibition of IL-1 beta induced E-selectin expression
"
NF-kappa B → IL-1 beta: "
Electrophoretic mobility shift assay revealed that TMS inhibited IL-1 beta induced NF-kappa B activation, which is essential for E-selectin expression
"
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