Gene interactions and pathways from curated databases and text-mining
Blood 1994, PMID: 7994029

Regulation of cytokine expression by interferon-alpha in human bone marrow stromal cells: inhibition of hematopoietic growth factors and induction of interleukin-1 receptor antagonist.

Aman, M J; Keller, U; Derigs, G; Mohamadzadeh, M; Huber, C; Peschel, C

We investigated the effects of interferon-alpha (IFN-alpha) on the expression of cytokines by human bone marrow stromal cells. Production of granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte-CSF (G-CSF), and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha, tumor necrosis factor (TNF), or lipopolysaccharide (LPS). Addition of IFN-alpha to such stimulated cultures resulted in a strong downregulation of mRNA expression of GM-CSF and IL-1 beta. Similarly, the protein levels of GM-CSF and IL-1 beta were significantly reduced by IFN-alpha, whereas G-CSF production was only moderately inhibited. In contrast, IFN-alpha markedly stimulated the production of IL-1 receptor antagonist (IL-1RA) by stromal cells. The inhibition of cytokine expression resulted in a reduced hematopoietic activity of stromal cells, indicated by a reduced proliferation of the factor dependent cell line MO7e on IFN-alpha-treated stromal cells. In the presence of cycloheximide (CHX), IFN-alpha failed to inhibit IL-1 mRNA expression, whereas the regulation of GM-CSF and IL-1RA by IFN-alpha was not affected. Our results indicate that the myelosuppressive effects of IFN-alpha, as observed in therapeutic applications or associated with viral infections, are, in part, indirectly mediated by inhibition of the paracrine production of hematopoietic growth factors.

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Text Mining Data

colony stimulating factor → tumor necrosis factor (TNF): " Production of granulocyte-macrophage colony stimulating factor ( GM-CSF ), granulocyte-CSF (G-CSF), and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha, tumor necrosis factor (TNF) , or lipopolysaccharide (LPS) "

GM-CSF → IL-1 alpha: " Production of granulocyte-macrophage colony stimulating factor ( GM-CSF ), granulocyte-CSF (G-CSF), and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha , tumor necrosis factor (TNF), or lipopolysaccharide (LPS) "

colony stimulating factor → lipopolysaccharide (LPS): " Production of granulocyte-macrophage colony stimulating factor ( GM-CSF ), granulocyte-CSF (G-CSF), and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha, tumor necrosis factor (TNF), or lipopolysaccharide (LPS) "

granulocyte-CSF (G-CSF) → tumor necrosis factor (TNF): " Production of granulocyte-macrophage colony stimulating factor ( GM-CSF ), granulocyte-CSF (G-CSF) , and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha, tumor necrosis factor (TNF) , or lipopolysaccharide (LPS) "

granulocyte-CSF (G-CSF) → IL-1 alpha: " Production of granulocyte-macrophage colony stimulating factor ( GM-CSF ), granulocyte-CSF (G-CSF) , and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha , tumor necrosis factor (TNF), or lipopolysaccharide (LPS) "

granulocyte-CSF (G-CSF) → lipopolysaccharide (LPS): " Production of granulocyte-macrophage colony stimulating factor ( GM-CSF ), granulocyte-CSF (G-CSF) , and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha, tumor necrosis factor (TNF), or lipopolysaccharide (LPS) "

interleukin-1 beta (IL-1 beta) → tumor necrosis factor (TNF): " Production of granulocyte-macrophage colony stimulating factor ( GM-CSF ), granulocyte-CSF (G-CSF), and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha, tumor necrosis factor (TNF) , or lipopolysaccharide (LPS) "

interleukin-1 beta (IL-1 beta) → IL-1 alpha: " Production of granulocyte-macrophage colony stimulating factor ( GM-CSF ), granulocyte-CSF (G-CSF), and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha , tumor necrosis factor (TNF), or lipopolysaccharide (LPS) "

interleukin-1 beta (IL-1 beta) → lipopolysaccharide (LPS): " Production of granulocyte-macrophage colony stimulating factor ( GM-CSF ), granulocyte-CSF (G-CSF), and interleukin-1 beta (IL-1 beta) in stromal cell layers was induced by incubation with IL-1 alpha, tumor necrosis factor (TNF), or lipopolysaccharide (LPS) "

Manually curated Databases

No curated data.