Cell 1994,
PMID: 8069916
Rothe, M; Wong, S C; Henzel, W J; Goeddel, D V
Mutational analysis identified a C-terminal region of 78 amino acids within the cytoplasmic domain of the human 75 kDa tumor necrosis factor receptor (TNF-R2) that is required for signal transduction. This region was subsequently shown to mediate the interaction of cytoplasmic factors with TNF-R2. Two of these factors were isolated and molecularly cloned using biochemical purification and the yeast two-hybrid system. TNF receptor-associated factor 1 (TRAF1) and TRAF2 are the first two members of a novel protein family containing a novel C-terminal homology region, the TRAF domain. In addition, TRAF2 contains an N-terminal RING finger motif. TRAF1 and TRAF2 can form homo- and heterotypic dimers. Our analysis indicates that TRAF1 and TRAF2 are associated with the cytoplasmic domain of TNF-R2 in a heterodimeric complex in which TRAF2 contacts the receptor directly. TRAF1 interacts with TNF-R2 indirectly through heterodimer formation with TRAF2.
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Text Mining Data
Dashed line = No text mining data
Manually curated Databases
-
IRef Biogrid Interaction:
TRAF2
—
TNFRSF1B
(physical association, affinity chromatography technology)
-
IRef Biogrid Interaction:
TRAF2
—
TNFRSF1B
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
TRAF1
—
TRAF1
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
TRAF1
—
TRAF2
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
TRAF2
—
TRAF2
(direct interaction, two hybrid)
-
IRef Biogrid Interaction:
TRAF1
—
TNFRSF1B
(direct interaction, two hybrid)
-
IRef Hprd Interaction:
TRAF1
—
TRAF2
(in vitro)
-
IRef Hprd Interaction:
TRAF1
—
TRAF2
(in vivo)
-
IRef Hprd Interaction:
TRAF1
—
TRAF2
(two hybrid)
-
IRef Hprd Interaction:
TRAF1
—
TRAF2
(in vivo)
-
IRef Hprd Interaction:
TRAF1
—
TRAF2
(two hybrid)
-
IRef Hprd Interaction:
TRAF2
—
TRAF2
(in vivo)
-
IRef Hprd Interaction:
TRAF2
—
TRAF2
(in vitro)
-
IRef Hprd Interaction:
TRAF2
—
TRAF2
(two hybrid)
-
IRef Intact Interaction:
TRAF2
—
TNFRSF1B
(physical association, two hybrid)
-
IRef Intact Interaction:
TRAF1
—
TRAF1
(physical association, two hybrid)
-
IRef Intact Interaction:
TRAF1
—
TRAF2
(physical association, two hybrid)
-
IRef Intact Interaction:
TRAF2
—
TRAF2
(physical association, two hybrid)
-
IRef Intact Interaction:
TRAF1
—
TNFRSF1B
(physical association, two hybrid)
-
IRef Ophid Interaction:
TRAF2
—
TNFRSF1B
(aggregation, confirmational text mining)
-
IRef Ophid Interaction:
TRAF1
—
TRAF2
(aggregation, confirmational text mining)
-
IRef Ophid Interaction:
TRAF1
—
TNFRSF1B
(aggregation, confirmational text mining)
-
NCI Pathway Database TNF receptor signaling pathway :
TNF-alpha/TNFR2 (cleaved) complex (TNFRSF1B-TNF)
→
TNF-alpha/TNFR2 (cleaved)/TRAF1/TRAF2 complex (TNFRSF1B-TRAF1-TRAF2-TNF)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database TNF receptor signaling pathway :
TNF-alpha/TNFR2 (cleaved) complex (TNFRSF1B-TNF)
→
TRAF1/TRAF2 complex (TRAF1-TRAF2)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database TNF receptor signaling pathway :
TNF-alpha/TNFR2 (cleaved)/TRAF1/TRAF2 complex (TNFRSF1B-TRAF1-TRAF2-TNF)
→
TRAF1/TRAF2 complex (TRAF1-TRAF2)
(modification, collaborate)
Evidence: mutant phenotype, physical interaction
In total, 10 gene pairs are associated to this article in curated databases