Nature 1993,
PMID: 8479541
Li, N; Batzer, A; Daly, R; Yajnik, V; Skolnik, E; Chardin, P; Bar-Sagi, D; Margolis, B; Schlessinger, J
Many of the actions of receptor tyrosine kinases are mediated by the protein Ras, including the activation of various downstream serine/threonine kinases and the stimulation of growth and differentiation. The human protein Grb2 binds to ligand-activated growth factor receptors and downstream effector proteins through its Src-homology (SH) domains SH2 and SH3, respectively, and like its homologue from Caenorhabditis elegans, Sem-5, apparently forms part of a highly conserved pathway by which these receptors can control Ras activity. Here we show that the SH3 domains of Grb2 bind to the carboxy-terminal part of hSos1, the human homologue of the Drosophila guanine-nucleotide-releasing factor for Ras, which is essential for control of Ras activity by epidermal growth factor receptor and sevenless. Moreover, a synthetic 10-amino-acid peptide containing the sequence PPVPPR specifically blocks the interaction. These results indicate that the Grb2/hSos1 complex couples activated EGF receptor to Ras signalling.
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Text Mining Data
Dashed line = No text mining data
Manually curated Databases
-
IRef Biogrid Interaction:
SOS1
—
GRB2
(physical association, affinity chromatography technology)
-
IRef Hprd Interaction:
SOS1
—
GRB2
(in vitro)
-
IRef Hprd Interaction:
SOS1
—
GRB2
(in vivo)
-
IRef Ophid Interaction:
GRB2
—
SOS1
(aggregation, confirmational text mining)
-
NCI Pathway Database Regulation of Ras family activation:
None
→
HRAS/GTP complex (HRAS)
(modification, collaborate)
Evidence: assay, physical interaction
-
NCI Pathway Database Regulation of Ras family activation:
None
→
GRB2/SOS1 complex (GRB2-SOS1)
(modification, collaborate)
Evidence: assay, physical interaction
-
NCI Pathway Database Regulation of Ras family activation:
None
→
HRAS/GDP complex (HRAS)
(modification, collaborate)
Evidence: assay, physical interaction
-
NCI Pathway Database Regulation of Ras family activation:
None
→
None
(modification, collaborate)
Evidence: assay, physical interaction
-
NCI Pathway Database Regulation of Ras family activation:
HRAS/GTP complex (HRAS)
→
HRAS/GDP complex (HRAS)
(modification, collaborate)
Evidence: assay, physical interaction
-
NCI Pathway Database Regulation of Ras family activation:
HRAS/GTP complex (HRAS)
→
None
(modification, collaborate)
Evidence: assay, physical interaction
-
NCI Pathway Database Regulation of Ras family activation:
GRB2/SOS1 complex (GRB2-SOS1)
→
HRAS/GDP complex (HRAS)
(modification, activates)
Evidence: assay, physical interaction
-
NCI Pathway Database Regulation of Ras family activation:
GRB2/SOS1 complex (GRB2-SOS1)
→
None
(modification, activates)
Evidence: assay, physical interaction
-
NCI Pathway Database Regulation of Ras family activation:
HRAS/GDP complex (HRAS)
→
None
(modification, collaborate)
Evidence: assay, physical interaction
-
NCI Pathway Database Regulation of Ras family activation:
None
→
KRAS family/GDP complex ()
(modification, collaborate)
Evidence: assay
-
NCI Pathway Database Regulation of Ras family activation:
None
→
GRB2/SOS1 complex (GRB2-SOS1)
(modification, collaborate)
Evidence: assay
-
NCI Pathway Database Regulation of Ras family activation:
None
→
None
(modification, collaborate)
Evidence: assay
-
NCI Pathway Database Regulation of Ras family activation:
None
→
KRAS family/GTP complex (KRAS)
(modification, collaborate)
Evidence: assay
-
NCI Pathway Database Regulation of Ras family activation:
KRAS family/GDP complex ()
→
GRB2/SOS1 complex (GRB2-SOS1)
(modification, collaborate)
Evidence: assay
-
NCI Pathway Database Regulation of Ras family activation:
KRAS family/GDP complex ()
→
None
(modification, collaborate)
Evidence: assay
-
NCI Pathway Database Regulation of Ras family activation:
KRAS family/GDP complex ()
→
KRAS family/GTP complex (KRAS)
(modification, collaborate)
Evidence: assay
-
NCI Pathway Database Regulation of Ras family activation:
GRB2/SOS1 complex (GRB2-SOS1)
→
None
(modification, activates)
Evidence: assay
-
NCI Pathway Database Regulation of Ras family activation:
GRB2/SOS1 complex (GRB2-SOS1)
→
KRAS family/GTP complex (KRAS)
(modification, activates)
Evidence: assay
-
NCI Pathway Database Regulation of Ras family activation:
None
→
KRAS family/GTP complex (KRAS)
(modification, collaborate)
Evidence: assay
-
Reactome Reaction:
GRB2
→
SOS1
(direct_complex)
-
Reactome Reaction:
GRB2
→
SOS1
(reaction)
In total, 6 gene pairs are associated to this article in curated databases