Thromb Haemost 1997,
Bierhaus, A; Zhang, Y; Quehenberger, P; Luther, T; Haase, M; Müller, M; Mackman, N; Ziegler, R; Nawroth, P P
The natural occurring pigment curcumin, a major component of the spice tumeric, has been described to have antioxidative, anti-tumorpromoting, anti-thrombotic and anti-inflammatory properties. It appears, that the pleiotropic effects of curcumin are at least partly due to inhibition of the transcription factors NF-kappa B and AP-1. This study investigates the effect of curcumin on the TNF alpha induced expression of endothelial Tissue Factor (TF), the central mediator of coagulation known to be controlled by AP-1 and NF-kappa B. When bovine aortic endothelial cells (BAEC) were preincubated in the presence of curcumin, TNF alpha induced TF gene transcription and expression were reduced. Transient transfection studies with TF-promoter plasmids revealed that both, NF-kappa B and AP-1 dependent TF expression, were reduced by curcumin action. The observed inhibitions were due to distinct mechanisms. Curcumin inhibited TNF alpha induced I kappa B alpha degradation and the nuclear import of NF-kappa B. In contrast, inhibition of AP-1 was due to a direct interaction of curcumin with AP-1-binding to its DNA binding motif. Thus, curcumin inhibits NF-kappa B and AP-1 by two different mechanisms and reduces expression of endothelial genes controlled by both transcription factors in vitro.
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Text Mining Data
AP-1 → NF-kappa B: " The dietary pigment curcumin reduces endothelial tissue factor gene expression by inhibiting binding of AP-1
to the DNA and activation
of NF-kappa B
TNF alpha → NF-kappa B: " Curcumin inhibited TNF alpha induced I kappa B alpha degradation and the nuclear import of NF-kappa B "
TNF alpha → I kappa B alpha: " Curcumin inhibited TNF alpha induced I kappa B alpha degradation and the nuclear import of NF-kappa B "
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