Nature 1997,
PMID: 9202126
Endo, T A; Masuhara, M; Yokouchi, M; Suzuki, R; Sakamoto, H; Mitsui, K; Matsumoto, A; Tanimura, S; Ohtsubo, M; Misawa, H; Miyazaki, T; Leonor, N; Taniguchi, T; Fujita, T; Kanakura, Y; Komiya, S; Yoshimura, A
The proliferation and differentiation of cells of many lineages are regulated by secreted proteins known as cytokines. Cytokines exert their biological effect through binding to cell-surface receptors that are associated with one or more members of the JAK family of cytoplasmic tyrosine kinases. Cytokine-induced receptor dimerization leads to the activation of JAKs, rapid tyrosine-phosphorylation of the cytoplasmic domains, and subsequent recruitment of various signalling proteins, including members of the STAT family of transcription factors, to the receptor complex. Using the yeast two-hybrid system, we have now isolated a new SH2-domain-containing protein, JAB, which is a JAK-binding protein that interacts with the Jak2 tyrosine-kinase JH1 domain. JAB is structurally related to CIS, a cytokine-inducible SH2 protein. Interaction of JAB with Jak1, Jak2 or Jak3 markedly reduces their tyrosine-kinase activity and suppresses the tyrosine-phosphorylation and activation of STATs. JAB and CIS appear to function as negative regulators in the JAK signalling pathway.
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Text Mining Data
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Manually curated Databases
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IRef Biogrid Interaction:
SOCS1
—
JAK2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SOCS1
—
JAK2
(direct interaction, pull down)
-
IRef Biogrid Interaction:
SOCS1
—
JAK2
(direct interaction, two hybrid)
-
IRef Hprd Interaction:
SOCS1
—
JAK2
(in vivo)
-
IRef Hprd Interaction:
SOCS1
—
JAK2
(in vitro)
-
IRef Hprd Interaction:
SOCS1
—
JAK2
(two hybrid)
-
NCI Pathway Database IL2-mediated signaling events:
IL2R gamma/JAK3/SOCS1 complex (IL2RG-JAK3-SOCS1)
→
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R gamma/JAK3/SOCS1 complex (IL2RG-JAK3-SOCS1)
→
IL2R alpha (IL2RA)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R gamma/JAK3/SOCS1 complex (IL2RG-JAK3-SOCS1)
→
IL2R beta/JAK1/LCK/SOCS3 complex (IL2RB-JAK1-LCK-SOCS3)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R gamma/JAK3/SOCS1 complex (IL2RG-JAK3-SOCS1)
→
IL2R beta/JAK1/LCK/SOCS1 complex (IL2RB-JAK1-LCK-SOCS1)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R gamma/JAK3/SOCS1 complex (IL2RG-JAK3-SOCS1)
→
IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R gamma/JAK3/SOCS1 complex (IL2RG-JAK3-SOCS1)
→
IL2 (IL2)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R gamma/JAK3/SOCS1 complex (IL2RG-JAK3-SOCS1)
→
IL2R gamma/JAK3 complex (IL2RG-JAK3)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R gamma/JAK3/SOCS1 complex (IL2RG-JAK3-SOCS1)
→
IL2R beta/JAK1/LCK/CISH complex (IL2RB-JAK1-LCK-CISH)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3)
→
IL2R alpha (IL2RA)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3)
→
IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3)
→
IL2 (IL2)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3)
→
IL2R gamma/JAK3 complex (IL2RG-JAK3)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R alpha (IL2RA)
→
IL2R beta/JAK1/LCK/SOCS3 complex (IL2RB-JAK1-LCK-SOCS3)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R alpha (IL2RA)
→
IL2R beta/JAK1/LCK/SOCS1 complex (IL2RB-JAK1-LCK-SOCS1)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R alpha (IL2RA)
→
IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R alpha (IL2RA)
→
IL2 (IL2)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R alpha (IL2RA)
→
IL2R gamma/JAK3 complex (IL2RG-JAK3)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R alpha (IL2RA)
→
IL2R beta/JAK1/LCK/CISH complex (IL2RB-JAK1-LCK-CISH)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK/SOCS3 complex (IL2RB-JAK1-LCK-SOCS3)
→
IL2R beta/JAK1/LCK/SOCS1 complex (IL2RB-JAK1-LCK-SOCS1)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK/SOCS3 complex (IL2RB-JAK1-LCK-SOCS3)
→
IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK/SOCS3 complex (IL2RB-JAK1-LCK-SOCS3)
→
IL2 (IL2)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK/SOCS3 complex (IL2RB-JAK1-LCK-SOCS3)
→
IL2R gamma/JAK3 complex (IL2RG-JAK3)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK/SOCS3 complex (IL2RB-JAK1-LCK-SOCS3)
→
IL2R beta/JAK1/LCK/CISH complex (IL2RB-JAK1-LCK-CISH)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK/SOCS1 complex (IL2RB-JAK1-LCK-SOCS1)
→
IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK/SOCS1 complex (IL2RB-JAK1-LCK-SOCS1)
→
IL2 (IL2)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK/SOCS1 complex (IL2RB-JAK1-LCK-SOCS1)
→
IL2R gamma/JAK3 complex (IL2RG-JAK3)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK/SOCS1 complex (IL2RB-JAK1-LCK-SOCS1)
→
IL2R beta/JAK1/LCK/CISH complex (IL2RB-JAK1-LCK-CISH)
(modification, inhibits)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK)
→
IL2 (IL2)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK)
→
IL2R gamma/JAK3 complex (IL2RG-JAK3)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK)
→
IL2R beta/JAK1/LCK/CISH complex (IL2RB-JAK1-LCK-CISH)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2 (IL2)
→
IL2R gamma/JAK3 complex (IL2RG-JAK3)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2 (IL2)
→
IL2R beta/JAK1/LCK/CISH complex (IL2RB-JAK1-LCK-CISH)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL2-mediated signaling events:
IL2R gamma/JAK3 complex (IL2RG-JAK3)
→
IL2R beta/JAK1/LCK/CISH complex (IL2RB-JAK1-LCK-CISH)
(modification, collaborate)
Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
-
NCI Pathway Database IL12-mediated signaling events:
SOCS1 (SOCS1)
→
IL12/IL12R/TYK2/JAK2/SOCS1 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2-SOCS1)
(modification, collaborate)
Evidence: physical interaction
-
NCI Pathway Database IL12-mediated signaling events:
SOCS1 (SOCS1)
→
IL12/IL12R/TYK2/JAK2 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2)
(modification, collaborate)
Evidence: physical interaction
-
NCI Pathway Database IL12-mediated signaling events:
IL12/IL12R/TYK2/JAK2/SOCS1 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2-SOCS1)
→
IL12/IL12R/TYK2/JAK2 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2)
(modification, collaborate)
Evidence: physical interaction
In total, 80 gene pairs are associated to this article in curated databases