Gene interactions and pathways from curated databases and text-mining
Science 1997, PMID: 9278512

Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216.

Peng, C Y; Graves, P R; Thoma, R S; Wu, Z; Shaw, A S; Piwnica-Worms, H

Human Cdc25C is a dual-specificity protein phosphatase that controls entry into mitosis by dephosphorylating the protein kinase Cdc2. Throughout interphase, but not in mitosis, Cdc25C was phosphorylated on serine-216 and bound to members of the highly conserved and ubiquitously expressed family of 14-3-3 proteins. A mutation preventing phosphorylation of serine-216 abrogated 14-3-3 binding. Conditional overexpression of this mutant perturbed mitotic timing and allowed cells to escape the G2 checkpoint arrest induced by either unreplicated DNA or radiation-induced damage. Chk1, a fission yeast kinase involved in the DNA damage checkpoint response, phosphorylated Cdc25C in vitro on serine-216. These results indicate that serine-216 phosphorylation and 14-3-3 binding negatively regulate Cdc25C and identify Cdc25C as a potential target of checkpoint control in human cells.

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Text Mining Data

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Manually curated Databases

  • IRef Hprd Interaction: PLK3 — CDC25C (in vitro)
  • IRef Hprd Interaction: PLK3 — CDC25C (in vivo)
  • IRef Hprd Interaction: CHEK1 — CDC25C (in vitro)
  • IRef Hprd Interaction: CHEK2 — CDC25C (in vitro)
  • IRef Hprd Interaction: YWHAH — CDC25C (in vivo)
  • IRef Hprd Interaction: YWHAH — CDC25C (in vitro)
  • IRef Hprd Interaction: MAPK14 — CDC25C (in vitro)
  • IRef Hprd Interaction: MARK3 — CDC25C (in vivo)
  • IRef Hprd Interaction: MARK3 — CDC25C (two hybrid)
  • IRef Hprd Interaction: MARK3 — CDC25C (in vitro)
  • NCI Pathway Database ATM pathway: CDC25C/YWHAB complex (CDC25C-YWHAB) → CDC25C (CDC25C) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database ATM pathway: CDC25C/YWHAB complex (CDC25C-YWHAB) → YWHAB (YWHAB) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database ATM pathway: CDC25C (CDC25C) → YWHAB (YWHAB) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database ATR signaling pathway: CDC25C/YWHAB complex (CDC25C-YWHAB) → CDC25C (CDC25C) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database ATR signaling pathway: CDC25C/YWHAB complex (CDC25C-YWHAB) → YWHAB (YWHAB) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database ATR signaling pathway: CDC25C (CDC25C) → YWHAB (YWHAB) (modification, collaborate)
    Evidence: physical interaction
  • Reactome Reaction: CHEK1 → CDC25C (reaction)
In total, 9 gene pairs are associated to this article in curated databases