Gene interactions and pathways from curated databases and text-mining
Mol Cell 1998, PMID: 9660950

Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300.

Zhong, H; Voll, R E; Ghosh, S

The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA). The transcriptional coactivator CPB/p300 associates with NF-kappa B p65 through two sites, an N-terminal domain that interacts with the C-terminal region of unphosphorylated p65, and a second domain that only interacts with p65 phosphorylated on serine 276. Accessibility to both sites is blocked in unphosphorylated p65 through an intramolecular masking of the N terminus by the C-terminal region of p65. Phosphorylation by PKA both weakens the interaction between the N- and C-terminal regions of p65 and creates an additional site for interaction with CBP/p300. Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300.

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Text Mining Data

NF-kappa B → protein kinase A (PKA): " The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA) "

NF-kappa B → PKA: " Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300 "

NF-kappa B — CBP/p300: " Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300 "

PKA — CBP/p300: " Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300 "

Manually curated Databases

  • IRef Hprd Interaction: RELA — PRKACA (in vitro)
  • IRef Hprd Interaction: RELA — PRKACA (in vivo)
  • IRef Ophid Interaction: CREBBP — RELA (aggregation, confirmational text mining)
  • NCI Pathway Database Canonical NF-kappaB pathway: IKK complex/PKC alpha complex (CHUK-IKBKB-IKBKG-PRKCA) → PKC alpha (PRKCA) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Canonical NF-kappaB pathway: IKK complex/PKC alpha complex (CHUK-IKBKB-IKBKG-PRKCA) → IKK complex complex (CHUK-IKBKB-IKBKG) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Canonical NF-kappaB pathway: PKC alpha (PRKCA) → IKK complex complex (CHUK-IKBKB-IKBKG) (modification, collaborate)
    Evidence: physical interaction
  • NCI Pathway Database Canonical NF-kappaB pathway: IKK complex/ELKS complex (CHUK-IKBKB-IKBKG-ERC1) → IKK complex/PKC alpha complex (CHUK-IKBKB-IKBKG-PRKCA) (modification, activates)
    Evidence: assay, physical interaction
  • NCI Pathway Database Canonical NF-kappaB pathway: IKK complex/ELKS complex (CHUK-IKBKB-IKBKG-ERC1) → NF kappa B1 p50/RelA/I kappa B alpha complex (NFKB1-NFKBIA-RELA) (modification, activates)
    Evidence: assay, physical interaction
  • NCI Pathway Database Canonical NF-kappaB pathway: IKK complex/ELKS complex (CHUK-IKBKB-IKBKG-ERC1) → IKK complex complex (CHUK-IKBKB-IKBKG) (modification, activates)
    Evidence: assay, physical interaction
  • NCI Pathway Database Canonical NF-kappaB pathway: IKK complex/PKC alpha complex (CHUK-IKBKB-IKBKG-PRKCA) → NF kappa B1 p50/RelA/I kappa B alpha complex (NFKB1-NFKBIA-RELA) (modification, activates)
    Evidence: assay, physical interaction
  • NCI Pathway Database Canonical NF-kappaB pathway: IKK complex/PKC alpha complex (CHUK-IKBKB-IKBKG-PRKCA) → IKK complex complex (CHUK-IKBKB-IKBKG) (modification, activates)
    Evidence: assay, physical interaction
In total, 34 gene pairs are associated to this article in curated databases