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NOS2 — TNF

Pathways - manually collected, often from reviews:

• WikiPathways Spinal Cord Injury: NOS2 → TNF (activation)

Text-mined interactions from Literome

Kapur et al., Am J Physiol 1999 (Endotoxemia) : However, when used in combination, TNF-alpha , IFN-gamma, and LPS markedly and synergistically increased iNOS activity in these cells
Hirji et al., Int Arch Allergy Immunol 1999 : In addition, Ro 31-8220 ( a PKC inhibitor ) inhibited OX8 induced iNOS upregulation, NO and IL-1beta production, but did not inhibit TNF-alpha production
Saura et al., J Mol Biol 1999 : We investigated the molecular mechanism for the synergistic induction of inducible nitric oxide synthase transcription by TNF-alpha and IFN-gamma
Lodato et al., Am J Physiol 1999 : We conclude that the LPS induced increase in NOS II activity and the decrease in ileal muscle contractility are mediated by TNF and IL-1
Liesenfeld et al., Parasite Immunol 1999 (Genetic Predisposition to Disease...) : Treatment with anti-TNF-alpha mAb did not affect the expression of IFN-gamma in the LPC but inhibited expression of iNOS in the infected mice, indicating the role of TNF-alpha in the induction of iNOS
Cain et al., Crit Care Med 1999 (Cardiomyopathies...) : We hypothesized the following : a ) TNF-alpha and IL-1beta directly depress human myocardial function ; b ) together, TNF-alpha and IL-1beta act synergistically to depress human myocardial function ; and c ) inhibition of ceramidase or nitric oxide synthase attenuates myocardial depression induced by TNF-alpha or IL-1beta by limiting proximal cytokine signaling or production of myocardial nitric oxide ( NO )
Nagasaki et al., Nephrol Dial Transplant 1999 : iNOS mRNA levels were increased by TNF-alpha , and were abruptly diminished after OP mRNA was significantly induced
Binder et al., Anticancer Res 1999 (Adenocarcinoma...) : Simultaneously, TNF alpha stimulated induction of iNOS and generation of NO
Wang et al., Infect Immun 1999 : Hepatocyte iNOS induced by recombinant mouse TNF-alpha was also inhibited by DEM treatment
Kan et al., Am J Physiol 1999 : The effects of tumor necrosis factor-alpha (TNF-alpha) on inducible NO synthase (iNOS) were not characterized
Wang et al., Hypertension 1999 (MAP Kinase Signaling System) : Salicylate treatment inhibited iNOS expression induced by TNF-alpha and IFN-gamma and attenuated the phosphorylation of ERK by TNF-alpha and IFN-gamma either alone or in combination
Yamaoka et al., J Dermatol Sci 2000 : Accordingly, we sought to determine in this study whether zinc ion may have effect on IFN-gamma or TNF-alpha induced NO production and iNOS expression in cultured murine keratinocytes
ter Steege et al., Eur Cytokine Netw 2000 : Therefore, in the present study, we investigated the role of endogenous IFN-gamma, TNF-alpha and IL-10 in the regulation of LPS induced tissue iNOS expression in the major organs ... Whereas blocking either IFN-gamma or TNF-alpha did not affect iNOS expression, iNOS enzymatic activity seems to be inhibited ... In contrast, blocking both mediators nearly completely prevents iNOS expression after LPS challenge, suggesting that the presence of either IFN-gamma or TNF-alpha is essential for LPS induced iNOS expression in these organs
Akama et al., J Biol Chem 2000 : Beta-amyloid stimulation of inducible nitric-oxide synthase in astrocytes is interleukin-1beta- and tumor necrosis factor-alpha (TNFalpha) dependent , and involves a TNFalpha receptor associated factor- and NFkappaB inducing kinase dependent signaling mechanism ... Here, we address the possibility that Abeta stimulated iNOS expression might result from an initial induction of IL-1beta and TNFalpha ... Our data suggest that Abeta stimulation of astrocyte iNOS is mediated in part by IL-1beta and TNFalpha , and involves a TRAF6-, TRAF2-, and NIK dependent signaling mechanism
Däubener et al., Adv Exp Med Biol 1999 : We were able to show that the IL-1 and TNF-alpha mediated inhibition of IFN-gamma induced IDO activity in uroepithelial cells is due to stimulation of inducible nitric oxide synthase
Song et al., Cardiovasc Res 2000 : TNF alpha induced a concentration dependent increase in iNOS mRNA expression and nitrite production as well as significant apoptosis of cardiomyocytes in the wild type mice ( n = 4, P < 0.01 )
Koyama et al., Eur J Pharmacol 2000 : We used the reverse transcriptase-polymerase chain reaction ( RT-PCR ) to identify the subtypes of NO synthase that were expressed in the ST2 cells and we detected the expression of an inducible NO synthase gene in response to tumor necrosis factor-alpha (TNF-alpha)
Chung et al., Immunopharmacol Immunotoxicol 2000 : Protein kinase C independent activation of inducible nitric oxide synthase by tumor necrosis factor-alpha in TM4 Sertoli cells
Iyer et al., J Biol Chem 2000 : Experiments with HO-1/KO and iNOS/KO mice showed that Allotrap 1258 mediated inhibition of TNF was independent of HO-1 and iNOS
Vann et al., J Biol Chem 2000 : Divergence in regulation of nitric-oxide synthase and its cofactor tetrahydrobiopterin by tumor necrosis factor-alpha ... Indeed, we found that bacterial sphingomyelinase, which hydrolyzes sphingomyelin and increases endogenous ceramide, or the cell permeable ceramide analogue, C ( 2 ) -ceramide, but not C ( 2 ) -dihydroceramide ( N-acetylsphinganine ), significantly mimicked the effects of TNF-alpha on NO production and iNOS expression and activity in C6 cells ... Treatment with the antioxidant pyrrolidine dithiocarbamate, which is known to inhibit NF-kappaB and sphingomyelinase in C6 cells, or with the peptide SN-50, which blocks translocation of NF-kappaB to the nucleus, inhibited TNF-alpha dependent iNOS mRNA expression without affecting GTPCH mRNA levels
Zhang et al., Br J Pharmacol 2000 : However, TNF-alpha alone did not induce iNOS promoter activity, protein expression, or nitrite production, indicating that NF-kappaB activation alone is not sufficient for iNOS induction
Chen et al., Hiroshima J Med Sci 2000 : Dexamethasone decreased CBF at a concentration of 10 ( -6 ) M. Fluorescent immunohistochemistry demonstrated that the expression of inducible NOS was augmented by TNF-alpha and attenuated by dexamethasone, whereas that of endothelial NOS remained unchanged
Barbaro et al., AIDS 2000 (AIDS Dementia Complex...) : In the relationship between cardiomyopathy and encephalopathy, the activation of iNOS by TNF-alpha may have a significant pathogenetic role in HIV disease
Harris et al., Prostate 2000 (Disease Models, Animal...) : After 4 weeks of treatment with E ( 2 ) + DHT, a significant increase in transcript levels of IL-4, IL-5, IL-6, MIP-2, eotaxin, and iNOS was detected , while IL-1beta and TNF-alpha transcript levels only increased slightly
Yamaoka et al., Arch Dermatol Res 2000 : Ultraviolet B radiation downregulates inducible nitric oxide synthase expression induced by interferon-gamma or tumor necrosis factor-alpha in murine keratinocyte Pam 212 cells ... UVBR also downregulated IFN-gamma- or TNF-alpha induced iNOS expression at both the mRNA level and the protein level ... These findings suggest the possibility that the down-regulatory effect of UVBR on IFN-gamma- or TNF-alpha induced iNOS expression may, in part, explain the antiinflammatory and therapeutic properties of UVBR in inflammatory dermatoses
Merial et al., Am J Physiol Cell Physiol 2000 : Moreover, Western blot analysis clearly showed that TNF-alpha alone induces the expression of iNOS after 12-24 h treatment of differentiated 3T3F442A cells
Macdonald et al., Neuroreport 2000 (Adrenal Gland Neoplasms...) : Tumor necrosis factor-alpha and nerve growth factor synergistically induce iNOS in pheochromocytoma cells ... Inducible nitric oxide synthase (iNOS) has been reported in tangle bearing neurons of patients with Alzheimer 's disease ( AD ), and can be induced by tumor necrosis factor-alpha (TNFalpha) ... In this study we determined the effect of TNFalpha on iNOS in NGF-responsive pheochromocytoma ( PC12 ) cells ... We found that while TNFalpha and NGF alone were unable to induce iNOS , their simultaneous addition resulted in iNOS induction and the release of nitric oxide ... Our results suggest that synergistic iNOS induction by TNFalpha and NGF may occur in selective population of NGF-responsive neurons in the presence of elevated CNS levels of TNFalpha
Combs et al., J Neurosci 2001 (Alzheimer Disease...) : beta-Amyloid stimulation of microglia and monocytes results in TNFalpha dependent expression of inducible nitric oxide synthase and neuronal apoptosis
Hattori et al., J Cell Physiol 2001 : Of note, inducible NO synthase (iNOS) and matrix metalloproteinase (MMP)-9 mRNAs were strongly stimulated by TNFalpha
Hoffmann et al., Crit Care Med 2001 (Bacteremia) : However, PCT ameliorated TNF-alpha/IFN-gamma induced iNOS gene expression in a dose dependent manner ( maximal inhibition at PCT 100 ng/mL by -66 % for 24 hrs and -80 % for 48 hrs )
Sass et al., J Clin Invest 2001 : In addition to their other functions, TNF-alpha and IFN-gamma both induce the inducible nitric oxide (NO) synthase ( iNOS )
Sanders et al., Perfusion 2001 (Myocardial Reperfusion Injury) : The hypothesis of this study was to determine if TNF-alpha would stimulate iNOS and its product nitric oxide ( NO ) similarly in immortalized macrophage and cardiac myocytes ... The expression of iNOS in the cardiac myocyte did not increase with TNF-alpha and LPS ... This study suggests that HL-1 myocyte iNOS can not be induced by TNF-alpha , unlike macrophage iNOS
Chen et al., Perit Dial Int 2000 : Both double combinations of TNFalpha + IFNgamma and IL-1beta + IFNgamma were able to induce iNOS mRNA expression, but only TNFalpha + IFNgamma induced significant NO production
González-Fernández et al., Atherosclerosis 2001 : Cerivastatin prevents tumor necrosis factor-alpha induced downregulation of endothelial nitric oxide synthase : role of endothelial cytosolic proteins
Ochoa et al., Ann Surg 2001 (Wounds and Injuries) : In contrast, nitric oxide synthase expression is induced by IL-1, tumor necrosis factor , and gamma interferon, a T-helper 1 cytokine profile
Bekker et al., J Immunol 2001 : Thus, iNOS expression appears to be TNF-alpha independent but iNOS generation of NO requires TNF-alpha
Kahn et al., J Exp Med 2001 (Encephalomyelitis, Autoimmune, Experimental) : Maximal expression of NOS2 after CFA immunization requires the presence of functional type I tumor necrosis factor alpha receptor ( TNFR1 ) and interferon gamma
Paludan et al., Eur Cytokine Netw 2001 : Interferon (IFN)-gamma and Herpes simplex virus/tumor necrosis factor-alpha synergistically induce nitric oxide synthase 2 in macrophages through cooperative action of nuclear factor-kappa B and IFN regulatory factor-1
Hurtado et al., Neuropharmacology 2001 (Brain Ischemia) : Taken together, these data demonstrate that ( 1 ) TACE/ADAM17 activity accounts for the majority of TNF-alpha shedding after OGD in rat forebrain slices, ( 2 ) an increase in TACE expression contributes , at least in part, to the rise in TNF-alpha after OGD and ( 3 ) iNOS expression in OGD subjected brain slices results from TACE activity and subsequent increase in TNF-alpha levels
Svensson et al., Microb Pathog 2001 : Furthermore, co-incubating S. typhimurium with Mphi resulted in interleukin (IL)-12p40, IL-6 and tumor necrosis factor-alpha production as well as iNOS induction while IL-12p70 was not detectable
Theas et al., Neuroendocrinology 2001 : Also, TNF-alpha induced iNOS gene expression in anterior pituitary cells as assessed by reverse transcriptase-polymerase chain reaction ... The current results indicate that NO is involved in the inhibitory effect of TNF-alpha on prolactin secretion and that TNF-alpha induces iNOS transcription and stimulates NO synthesis in anterior pituitary cells
Sekine et al., Endocrinology 2001 : This action of GH may be sufficient to suppress the synergistic induction of inducible nitric oxide synthase by interferon-gamma and TNFalpha , thereby preventing the cytotoxicity to beta-cells
Chou et al., Naunyn Schmiedebergs Arch Pharmacol 2001 (Endotoxemia) : However, incubation with HCL-31D ( 1 approximately 50 microM ) for 24 h caused significant attenuation of nitrite and TNF-alpha formation as well as iNOS mRNA induction in a dose dependent manner but no effect on iNOS activity in RASMC ... These effects may be mainly due to inhibition of TNF-alpha formation and of the induction of iNOS ... We proposed that the elevation of cAMP levels by HCL-31D may be involved in the prevention of TNF-alpha formation and iNOS induction
Olinga et al., J Hepatol 2001 (Drug-Induced Liver Injury...) : In addition, it is concluded that the upregulation of iNOS in hepatocytes by LPS is caused by cytokines produced by Kupffer cells because inhibition of TNFalpha and IL-1beta production attenuated iNOS induction
Kwon et al., Nitric Oxide 2001 : We found that both IL-1beta and TNF-alpha could independently activate cytosolic NF-kappaB, direct its translocation into the nucleus, and induce iNOS monomer synthesis
Zulueta et al., Am J Respir Cell Mol Biol 2002 (Anoxia) : EC did not express NOS 2 mRNA or protein when exposed to normoxia or hypoxia unless they were pretreated with interleukin (IL)-1beta and/or tumor necrosis factor (TNF)-alpha for 24 h. Induction of NOS 2 by IL-1beta+TNF-alpha was significantly attenuated by concomitant exposure of EC to hypoxia or treatment of EC with antioxidants such as tiron, diphenyliodonium, and catalase, suggesting that NOS 2 expression is dependent on the production of reactive oxygen species ... As opposed to the attenuating effect that hypoxia had on IL-1beta+TNF-alpha- dependent induction of NOS 2 gene expression, the concomitant treatment with IL-1beta+TNF-alpha and hypoxia synergistically increased NOS 2 promoter activity 17.6-fold
Enkhbaatar et al., Thromb Haemost 2001 (Hypotension...) : Tumor necrosis factor-alpha (TNF-alpha) induces iNOS , thereby contributing to the development of shock
Madrigal et al., Biol Psychiatry 2002 : In order to study the role of TACE and TNF-alpha in iNOS induction, a group of animals were i.p. injected with the preferred TACE inhibitor BB1101 ( 2 and 10 mg/kg )
Vann et al., J Biol Chem 2002 (Glioma) : In C6 glioma cells, the sphingolipid second messenger ceramide potentiates expression of inducible nitric-oxide synthase (iNOS) induced by tumor necrosis factor alpha (TNF-alpha) without affecting GTP cyclohydrolase I (GTPCH), the rate limiting enzyme in the biosynthesis of 6 ( R ) -5,6,7,8-tetrahydrobiopterin ( BH ( 4 ) ), a cofactor required for iNOS activity
Hilfiker-Kleiner et al., Cardiovasc Res 2002 : In the myocardium, TNFalpha enhances the expression of inducible nitric oxide synthase (iNOS)
Gayle et al., Brain Res Dev Brain Res 2002 (Encephalitis...) : Inhibition of the inducible form of nitric oxide synthase ( iNOS ) by L-NIL did not affect LPS toxicity, but increased the LPS induced levels of both TNF-alpha and IL-1beta
Thomas et al., Pancreas 2002 (Adenocarcinoma...) : Further, inhibition of nitric oxide ( NO ) synthase by L-NAME potentiated the TNF-alpha response, suggesting a protective role for endogenously produced NO in these two cell lines
Aljada et al., Metabolism 2002 : Tumor necrosis factor-alpha inhibits insulin induced increase in endothelial nitric oxide synthase and reduces insulin receptor content and phosphorylation in human aortic endothelial cells
Ribeiro et al., J Urol 2002 (Cystitis...) : The induction of inducible nitric oxide synthase in the urothelium appears to depend on the synergistic effect of IL-1beta and TNF-alpha
Wagner et al., Br J Pharmacol 2002 : Western blot analysis and immunohistochemistry revealed that tumour necrosis factor alpha (TNFalpha) plus interferon-gamma (IFNgamma) synergistically induce iNOS gene expression in the endothelium but not in the smooth muscle of these segments while constitutive endothelial NO synthase (eNOS) abundance was markedly reduced
Sass et al., Inflamm Res 2002 (Drug-Induced Liver Injury) : TNF-pretreatment did not affect TNFR-1 expression in the liver and resulted in time dependent up-regulation of iNOS , IL-1beta and HO-1 ... IL-1beta, iNOS and HO-1 neither mediated TNF-tolerance nor TNF cytotoxicity
Fecker et al., J Invest Dermatol 2002 (Breast Neoplasms...) : Inducible nitric oxide synthase is expressed in normal human melanocytes but not in melanoma cells in response to tumor necrosis factor-alpha , interferon-gamma, and lipopolysaccharide
Keil et al., Kidney Int 2002 (Fibrosis...) : The induction of the inducible NO-synthase by TNF-alpha , IL-1beta and LPS provoked a transient down-regulation of CTGF mRNA, an effect that could be partially overcome by pretreatment with the NOS-inhibitor Nomega-nitro-l-arginine methyl ester
Chou et al., Jpn J Pharmacol 2002 : The present study was to investigate the effect of a calcium antagonist amlodipine on nitrite, tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) formation and iNOS induction both in lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) treated rat aortic smooth muscle cells ( RASMC ) and in a rat model of endotoxemia
De Plaen et al., Immunology 2002 : Both LPS and TNF induce the expression of the NF-kappaB dependent gene inducible nitric oxide synthase (iNOS) , which occurs subsequent to NF-kappaB activation
Chanani et al., Circulation 2002 (Inflammation) : Both LPS and TNF-alpha induced significant NFkappaB activation, cyclooxygenase-2 (COX-2) expression, and inducible NO synthase (iNOS) and cytokine production ; with the exception of COX-2 expression, all were significantly reduced by amrinone, beginning at concentrations of 10 to 50 micro mol/L
Tian et al., Am J Physiol Heart Circ Physiol 2002 : In cultured adult rat CFbs, IL-1beta ( 5 ng/ml ), but not interferon-gamma ( 10 ng/ml ) or tumor necrosis factor-alpha ( 10 ng/ml ), induced inducible NO synthase (iNOS) expression and NO production that was associated with an increase in caspase-3 activity and apoptotic cell death
Knuefermann et al., Circulation 2002 (Inflammation...) : Myocardial tumor necrosis factor , interleukin-1beta (IL-1beta), and NOS2 induction was measured before and 6 hours after LPS challenge
Yang et al., Exp Mol Med 2002 : The present study was undertaken to explore whether retinoids, which are known to have immunomodulatory actions, could attenuate tumor necrosis factor-alpha (TNF) stimulated inducible nitric oxide synthase (iNOS) expression in 3T3-L1 adipocytes ... The inhibitory effect of RA on TNF induced iNOS induction was reversible, completely recovered after 2 days, and was exerted through the inhibition of NF-kappaB activation
Shinoda et al., Glia 2003 (Glioma) : Although TNFalpha alone induced neither iNOS mRNA expression nor nitrite formation, it significantly potentiated the effect of LPS on both
Yao et al., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2003 (Arthritis, Rheumatoid) : Results showed that neither FK506 nor TP at lower concentration ( 10 microg/L ) alone affected TNF alpha induced COX-2, iNOS expression and production of PGE2, NO in synovial cells
Vallés et al., Kidney Int 2003 (Ureteral Obstruction) : A role of tumor necrosis factor-alpha (TNF-alpha) on enhanced iNOS was suggested by the finding of increased urine levels in obstructed pelvis
Barsacchi et al., Mol Pharmacol 2003 : Activation of endothelial nitric-oxide synthase by tumor necrosis factor-alpha : a novel pathway involving sequential activation of neutral sphingomyelinase, phosphatidylinositol-3 ' kinase, and Akt
Yune et al., J Neurotrauma 2003 (Spinal Cord Injuries) : These data suggest that after SCI, apoptosis induced by TNF-alpha may be mediated in part by NO via upregulation of iNOS , induced in response to TNF-alpha
Frost et al., Endocrinology 2003 : TNF alpha increased both nitric oxide synthase-II mRNA and protein, and the nitric oxide donor sodium nitroprusside decreased IGF-I mRNA levels in C2C12 cells
Gottstein et al., Br J Nutr 2003 (Arteriosclerosis) : In a macrophage cell line ( RAW 264.7 ) activated with interferon gamma plus lipopolysaccharide, both isoflavones were found to inhibit NO production and tumour necrosis factor alpha (TNF-alpha) secretion dose-dependently, but they did not affect mRNA levels for inducible nitric oxide synthase and cyclo-oxygenase-2
Neu et al., Biochem Pharmacol 2003 : Investigation of downstream signaling pathways of apoptosis revealed that TNF-alpha induced the expression of iNOS , but failed to stimulate the activity of caspase 3
Hida et al., Free Radic Biol Med 2003 : We conclude that colloidal-iron phagocytosed by liver Kupffer cells enhanced LPS induced NO production in vivo, iNOS induction in the liver, and release of IL-6, IL-1beta, and TNF-alpha
Mérial-Kieny et al., Br J Pharmacol 2003 : Endothelin-1 inhibits TNF alpha induced iNOS expression in 3T3-F442A adipocytes ... In isolated adipocytes, TNFalpha induces the expression of the inducible nitric oxide synthase (iNOS) ... The purpose of the present work was, in the 3T3-F442A adipocyte cell line, to characterise TNFalpha induced iNOS expression and to determine whether or not ET-1 could influence TNFalpha induced iNOS expression and NO production ... TNFalpha induced iNOS expression requires nuclear factor kappaB activation, but does not necessitate the activation of the PI-3 kinase/Akt and P38-MAP kinase pathways ... 3. ET-1, but not ET-3, inhibited the TNFalpha induced expression of iNOS protein and mRNA as well as nitrite production ... 6. Thus, these results confirm that adipocytes are a target for circulating ET-1 and demonstrate that the activation of the ETA receptor subtype can prevent TNFalpha induced iNOS expression
Shin et al., Exp Mol Med 2003 : Earlier study showed that glucocorticoid induced tumor necrosis factor receptor ( GITR ), a new TNFR family, activated murine macrophages to express inducible nitric oxide synthase (iNOS) and to generate nitric oxide ( NO )
Fonseca et al., Eur J Immunol 2003 (Leishmaniasis, Cutaneous) : TNF-alpha mediates the induction of nitric oxide synthase in macrophages but not in neutrophils in experimental cutaneous leishmaniasis
Lee et al., Mol Cells 2003 : Astaxanthin also suppressed the serum levels of NO, PGE2, TNF-alpha , and IL-1beta in LPS administrated mice, and inhibited NF-kappaB activation as well as iNOS promoter activity in RAW264.7 cells stimulated with LPS
Paz et al., J Am Coll Cardiol 2003 (Disease Models, Animal...) : Effect of tumor necrosis factor-alpha on endothelial and inducible nitric oxide synthase messenger ribonucleic acid expression and nitric oxide synthesis in ischemic and nonischemic isolated rat heart
Lozano et al., Scand J Gastroenterol 2003 (Disease Models, Animal...) : In a previous study, we observed that enhancement of TNF-alpha expression in hepatocytes by prostaglandin E1 ( PGE1 ) pre-administration induced iNOS expression and cytoprotection against experimental liver injury in rats
Thomas et al., Diabetes 2004 (Diabetes Mellitus, Type 1...) : IL-1R-deficient islets were protected from the damaging effects of tumor necrosis factor (TNF) and interferon (IFN)-gamma in vitro, and beta-cell expression of iNOS was reduced, suggesting that IL-1 mediates the induction of iNOS by TNF and IFN-gamma
Dhar et al., Mol Cancer Ther 2003 (Cell Transformation, Neoplastic) : Tumor necrosis factor alpha (TNFalpha) induces iNOS and NO production in transformation-sensitive JB6 P+, but not in transformation-resistant JB6 P-, mouse epidermal cells
Browner et al., Am J Physiol Cell Physiol 2004 : IL-Ibeta, TNF-alpha , and LPS increased inducible nitric oxide synthase (iNOS) expression and cGMP production
Yu et al., Int Immunopharmacol 2004 : The present investigation demonstrated that oligochitosan can significantly increase the activity of inducible nitric oxide synthase (iNOS) and induce the synthesis of nitric oxide ( NO ) and tumor necrosis factor-alpha (TNF-alpha) in macrophages
Ferret-Bernard et al., Immunol Lett 2004 : As tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) contributed crucially to the activation of inducible NO synthase (iNOS) gene transcription and to the differentiation of macrophages, we tested their roles in the induction of iMacs differentiation from bone marrow hematopoietic progenitor cells ( HPC ) of uncompromised mice
Eum et al., Br J Pharmacol 2004 (Reperfusion Injury) : 6. The expression of TNF-alpha and iNOS mRNAs increased after I/R. Trolox inhibited increase in iNOS mRNA expression
Sztrymf et al., Eur Respir J 2004 (Hepatopulmonary Syndrome...) : Since TNF-alpha is a potent activator of macrophagic inducible nitric oxide synthase (NOS) , the aim of this study was to investigate whether TNF-alpha inhibition prevented hepatopulmonary syndrome and hyperdynamic circulatory state in rats with cirrhosis
Leindler et al., Pancreas 2004 (Ischemia...) : The aim of our study was to determine the production of cytokines, tumor necrosis factor (TNF) and interleukin-6 (IL-6), the activation of the inducible nitric oxide synthase (iNOS) , and the development of apoptosis during this pathologic process
Lee et al., Exp Cell Res 2004 (Bone Resorption) : In this study, we found that TNF-alpha , but not receptor activator of nuclear factor kappa B ligand and interleukin 1, increased the expression of iNOS both at the mRNA and protein levels
Candolfi et al., Neuroendocrinology 2004 : In conclusion, our study shows that TNF-alpha upregulates iNOS gene expression whereas it downregulates estrogen induced eNOS expression in anterior pituitary cells
Mocellin et al., Clin Cancer Res 2004 (Melanoma...) : We investigated the role of endothelial nitric oxide synthase in determining the tumor-selective activity of TNF
Fermor et al., Osteoarthritis Cartilage 2004 : TNF-alpha prevented the mechanical stimulation of proteoglycan synthesis, and this effect was not dependent on NOS2
Kurtovic et al., Eur J Gastroenterol Hepatol 2004 (Endotoxemia...) : The production of large amounts of nitric oxide, consequent to endotoxin induced tumour necrosis factor (TNF)-alpha mediated upregulation of inducible nitric oxide synthase (iNOS) , has been suggested to be central to this phenomenon ... Terlipressin has recently been shown in an animal model of cirrhosis to suppress endotoxin induced TNF-alpha mediated upregulation of iNOS , thereby preventing overproduction of nitric oxide and restoring normal vascular tone
Ortega-Gutiérrez et al., FASEB J 2005 (Multiple Sclerosis) : Additionally, in microglial cells, UCM707 decreases the production of the proinflammatory cytokines tumor necrosis factor (TNF)-alpha , interleukin (IL)-1beta, and IL-6 ; reduces nitric oxide levels and inducible nitric oxide synthase expression ; and is able to potentiate the action of a subeffective dose of the endocannabinoid anandamide
Yang et al., Ann N Y Acad Sci 2005 : Upon stimulation with tumor necrosis factor-alpha (TNF-alpha) plus interferon-gamma (IFN-gamma), antisense transfectants showed less iNOS enzymatic activity with less nitric oxide ( NO ) when compared to the sense control cells
Jörns et al., Diabetes 2005 (Diabetes Mellitus, Type 1) : The observed coincidence of IL-1beta and TNF-alpha expression in the immune cells and the induction of iNOS and procaspase 3 mRNA expression in the beta-cells depicts a sequence of pathological changes leading to apoptotic beta-cell death in the IDDM rat
Zeng et al., J Neurochem 2005 (Infection) : Amyloid-beta peptide enhances tumor necrosis factor-alpha induced iNOS through neutral sphingomyelinase/ceramide pathway in oligodendrocytes ... In order to study the roles of the amyloid-beta peptide in inducing oxidative stress damage in white matter of AD, we investigated the effects of amyloid-beta peptide 25-35 ( Abeta ) on proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) induced inducible nitric oxide synthase (iNOS) in cultured oligodendrocytes ( OLGs ) ... Although Abeta 25-35 by itself had little effect on iNOS mRNA, protein, and nitrite production, it enhanced TNF-alpha induced iNOS expression and nitrite generation in OLGs ... Cell permeable C2-ceramide enhanced TNF-alpha induced iNOS expression and nitrite generation ... These results suggest that neutral sphingomyelinase/ceramide pathway is required but may not be sufficient for iNOS expression induced by TNF-alpha and the combination of TNF-alpha and Abeta
Yamane et al., Microbes Infect 2005 : Although TNF-alpha is one of the iNOS-inducible factors, the iNOS up-regulation was not regulated by TNF-alpha
Blanco et al., Mitochondrion 2004 : The pathogenesis of OA includes elaboration of increased amounts of NO as a consequence of up-regulation of chondrocyte-inducible NO synthase induced by IL-1, TNF-alpha and other factors
Saud et al., Dig Dis Sci 2005 (Ileitis...) : TNF-alpha , including other proinflammatory cytokines alone or in combination, induces iNOS expression and upregulates inflammatory responses ... Although indomethacin significantly increased serum TNF-alpha and nitrate/nitrite levels over the control value as early as 12 hr, iNOS expression was detected only after 4 days
Thomas et al., Journal of neuroinflammation 2005 : We previously showed in NGF-responsive PC12 cells that tumor necrosis factor alpha (TNFalpha) and NGF synergistically induce the expression of the free-radical producing enzyme inducible nitric oxide synthase (iNOS)
Louis et al., Vet J 2006 (Dog Diseases...) : A higher level of NO was produced when CCLs were exposed to the inducible nitric oxide synthase (iNOS) inducing cocktail TNF/IL-1/LPS , and NO synthesis could be inhibited by both l-NMMA, a general nitric oxide synthase (NOS) inhibitor and l-NIL, a specific iNOS inhibitor
Tanimoto et al., Circ J 2005 (Ventricular Dysfunction, Left) : Forced expression of SOCS1 significantly inhibited iNOS transcription induced by LPS, tumor necrosis factor-alpha or interferon-gamma
de Souza et al., Mol Cell Biochem 2006 : Both TNF-alpha and inosine increased nitrite accumulation and nitric oxide synthase activity ... Erythro-9- ( 2-hydroxy-3-nonyl ) adenine ( EHNA ), an adenosine deaminase inhibitor, abolished the TNF-alpha induced inosine increase, nitrite accumulation and nitric oxide synthase activity
Dekker et al., Blood 2006 : Protein levels for thrombomodulin, endothelial nitric oxide synthase , and plasminogen activator inhibitor type-1 are altered to atheroprotective levels, even in the presence of the inflammatory cytokine TNF-alpha
Zheng et al., J Biol Chem 2006 (Bone Resorption) : Using murine RAW 264.7 and primary bone marrow cells or osteoclasts formed from them by receptor activator of NF-kappaB ligand ( RANKL ) differentiation, we found that inducible nitric-oxide synthase (iNOS) expression and NO generation were stimulated by interferon (IFN)-gamma or lipopolysaccharide, but not by interleukin-1 or tumor necrosis factor-alpha
Elahi et al., Exp Clin Transplant 2006 (Vascular Diseases) : The role of tumor necrosis factor-alpha in mediating pathophysiological activity of inducible nitric oxide synthase during transplant vasculopathy remains contentious
Lozano et al., Surgery 2006 (Systemic Inflammatory Response Syndrome) : This contamination coincided with an increase in TNF-alpha and with a greater expression of iNOS
Vila-del Sol et al., J Leukoc Biol 2007 : Taken together, our data suggest that the TNF-alpha produced in response to IFN-gamma is required for iNOS induction by activating NF-kappaB transcription factor
Ulanova et al., Biochem Biophys Res Commun 2006 : Stimulation of human bronchial EC line HS-24 by TNF caused an increased expression of inducible nitric oxide synthase (iNOS)
Hsiao et al., Glia 2007 (Calcium Signaling...) : TNF-alpha/IFN-gamma induced iNOS expression increased by prostaglandin E2 in rat primary astrocytes via EP2 evoked cAMP/PKA and intracellular calcium signaling
Tripathi et al., Mol Immunol 2007 (MAP Kinase Signaling System) : Similarly, there is no autocrine or paracrine effect of TNF-alpha on GH or PRL induced NO production and iNOS expression
Ziesché et al., J Biol Chem 2007 (Colonic Neoplasms...) : IL-22 did not stabilize IL-1beta/tumor necrosis factor-alpha/IFNgamma induced iNOS mRNA
Lee et al., J Mol Biol 2007 : Although STAT1 activation and expression did not change significantly in TNF-alpha/IFN-gamma cotreated cells compared with cells treated with IFN-gamma alone, the absence of STAT1 or interferon regulatory factor 1 (IRF-1) in genetic knockout mice strongly abrogated the observed effects of TNF-alpha/IFN-gamma on iNOS/NO induction, ROS production, loss of mitochondrial transmembrane potential ( DeltaPsim ), and apoptosis compared with STAT1 ( +/+ ) and IRF-1 ( +/+ ) mice ... Additionally, the synergistic effects of TNF-alpha/IFN-gamma on iNOS/NO induction, ROS production, and apoptosis were significantly inhibited by overexpression of dominant negative STAT1 in contrast to overexpression of wild-type STAT1
Skorokhod et al., Malaria journal 2007 (Malaria) : Malarial pigment haemozoin, IFN-gamma, TNF-alpha , IL-1beta and LPS do not stimulate expression of inducible nitric oxide synthase and production of nitric oxide in immuno purified human monocytes
Hatano et al., J Gastroenterol Hepatol 2007 (Liver Diseases) : The TNF-alpha and Jo2 induced iNOS through NF-kappaB
Gharavi et al., J Pharm Sci 2007 (Carcinoma, Hepatocellular...) : Furthermore, TNF-alpha and LPS significantly induced NOS2 expression
Takano et al., Biochem Biophys Res Commun 2007 : We found that, in glomerular podocytes, induction of monocyte chemoattractant protein 1 ( MCP-1 ) and inducible nitric oxide synthase (iNOS) by TNF-alpha was abrogated by K-7174 ... Furthermore, K-7174 elicited UPR abrogated induction of MCP-1 and iNOS not only by TNF-alpha but also by medium conditioned by activated macrophages
Momi et al., Eur J Pharmacol 2007 (Hyperlipidemias...) : In lipopolysaccharide from Escherichia coli ( LPS ) -treated RAW 264.7 macrophages, NCX 6560 reduced iNOS expression and dimer assembly more efficiently than atorvastatin and inhibited nitrite accumulation ( IC ( 50 ) =6.7+/-1.6 microM ) and TNFalpha release
Arai et al., Acta Otolaryngol 2008 : Induction of inducible nitric oxide synthase and apoptosis by LPS and TNF-alpha in nasal microvascular endothelial cells ... The expression of iNOS induced by both the LPS and TNF-alpha was investigated by fluorescent immunohistochemistry, using confocal laser microscopy ... The fluorescent immunohistochemistory study demonstrated that LPS and TNF-alpha induced the expression of iNOS in HNMECs ... LPS/TNF-alpha remarkably augmented the expression of iNOS in HNMECs in comparison to stimulation by either LPS or TNF-alpha alone
Kim et al., J Ethnopharmacol 2007 (Atherosclerosis) : In lipopolysaccharide (LPS) stimulated RAW264.7 cells, HMCO5 inhibited NF-kappaB activation as well as iNOS promoter activity, inhibited the secretion of TNF-alpha and IL-1beta, and directly inhibited the intracellular accumulation of reactive oxygen species
Kohjitani et al., Neurosci Lett 2008 (Glioma) : Induction of inducible NOS by TNF-alpha in combination with lipopolysaccharide (LPS) dramatically attenuated SULT2B1a expression ; this was partially reversed by the specific inducible NOS inhibitor N ( 6 ) - ( 1-iminoethyl ) -L-lysine hydrochloride ( L-NIL )
Juan et al., Obesity (Silver Spring) 2008 : The effects of TNF-alpha on inducible NOS (iNOS) protein expression in adipocytes were also measured by western blotting ... This effect seemed to be associated with the TNF-alpha induced expression of iNOS ... The results showed that TNF-alpha induced iNOS expression and release of NO after 24-h treatment of differentiated 3T3-L1 adipocytes ... In addition, pretreatment with protein tyrosine kinase ( PTK ) inhibitors, genistein and AG-1288, prevented TNF-alpha induced iNOS expression and subsequent resistin downregulation
Isakovic et al., Eur J Pharmacol 2008 (Glioma) : However, blockade of TNF-alpha action and COX-2 activity with anti-TNF-alpha antibodies and indomethacin, respectively, revealed that modulation of TNF-alpha and COX-2 was not involved in adenosine mediated iNOS suppression
Park et al., Cell Biol Int 2009 : Interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) induced iNOS gene expression and NO production, although these actions were inhibited by L-NG-monomethylarginine ( L-NMMA ) and decreased alkaline phosphatase ( ALPase ) activity
Wu et al., Free Radic Biol Med 2009 (MAP Kinase Signaling System) : TNFalpha plus PY treatment elevated 3-nitrotyrosine adduct formation and induced NOS2 in the liver ; 1400W and NAC blocked these changes
Erdman et al., Proc Natl Acad Sci U S A 2009 (Colonic Neoplasms...) : The presence of Gr-1 ( + ) neutrophils and elevated tumor necrosis factor-alpha (TNF-alpha) expression in colon were required for increased iNOS expression and cancer, whereas interleukin-10 (IL-10) down-regulated TNF-alpha and iNOS expression and suppressed cancer
Mahattanadul et al., Phytomedicine 2009 (Disease Models, Animal...) : Western blot analysis of mouse macrophage cell line RAW 264.7 activated with lipopolysaccharide showed that bisdemethoxycurcumin inhibited inducible nitric oxide synthase (iNOS) production significantly but had no effect on tumor necrosis factor-alpha (TNF-alpha) production, whereas curcumin showed stronger suppression of iNOS protein production and inhibited TNF-alpha protein production significantly
Xu et al., Stem Cells 2009 : C/EBPbeta knockdown also inhibited the synergistic expression of CXCL1, inducible nitric oxide synthase , and CCL5 in response to concomitant IFNgamma and TNFalpha
Du et al., Cancer Res 2009 (Neoplasms) : Furthermore, SW480 cells stably transformed with wild-type adenomatous polyposis coli showed decreased beta-catenin protein and increased TNFalpha induced p65 NF-kappaB binding as well as iNOS and Traf1 expression
Gonzalez-Gay et al., Clin Exp Rheumatol 2009 (Arthritis, Rheumatoid...) : TNF-alpha increases expression of inducible nitric oxide synthase (iNOS) in macrophages and vascular endothelial cells
Han et al., Korean J Parasitol 2009 (Trichomonas Infections) : However, trichomonad induced NF-kappaB activation and TNF-alpha production in macrophages were significantly inhibited by inhibition of iNOS levels with L-NMMA ( NO synthase inhibitor )
Ishii-Yonemoto et al., Am J Physiol Endocrinol Metab 2010 (Inflammation...) : In contrast, overexpression of 11 beta-HSD1 further augmented TNF-alpha induced iNOS , IL-6, and MCP-1 expression
Matsui et al., J Ethnopharmacol 2009 : Aqueous extract of Vitex trifolia leaves showed significant dose- and time dependent inhibitory activity on interleukin (IL)-1 beta, IL-6 and iNOS mRNA synthesis, but slight effect on tumor necrosis factor (TNF)-alpha , all of which are involved in the inflammatory response
Lien et al., Endocrinology 2009 : Suppression of TNF-alpha induced iNOS expression using short hairpin RNA significantly reduced TNF-alpha induced lipolysis
Bhatt et al., Mol Immunol 2010 : No paracrine or autocrine effect of TNF-alpha on PGN induced iNOS expression and NO release was observed
Tsou et al., J Cell Biochem 2010 : Integrin linked kinase is involved in TNF-alpha induced inducible nitric-oxide synthase expression in myoblasts ... TNF-alpha stimulation caused iNOS expression and NO production in myoblasts ( G7 cells ) ... TNF-alpha mediated iNOS expression was attenuated by integrin linked kinase (ILK) inhibitor ( KP392 ) and siRNA ... Our results suggest that TNF-alpha increased iNOS expression and NO production in myoblasts via the ILK/Akt/mTOR and NF-kappaB signaling pathway
Harada et al., Cell Death Differ 2010 (Disease Models, Animal...) : Tumor necrosis factor (TNF) induced activation of p38 MAPK and the production of inducible nitric oxide synthase were suppressed in ASK1-deficient Müller glial cells
Bai-Feng et al., Diabetes Res Clin Pract 2010 : The expression of iNOS and the promoting apoptosis gene Bax and Fas were significantly up-regulated by the induction of IL-1beta and TNF-alpha
Fu et al., Journal of neuroinflammation 2010 : This activation of IDO by direct application of LPS was preceded by synthesis and secretion of TNFalpha and IL-6 protein and activation of iNOS while IFN gamma expression was undetectable
Zhao et al., Mol Biol Rep 2012 (Glomerulonephritis) : Aldose reductase regulates TNF-a induced inducible nitric oxide synthase expression in human mesangial cells ... Here, we report our finding that AR regulates tumor-necrosis-factor-a induced ( TNF-a induced ) iNOS expression in human mesangial cells ( HMC ) via nuclear factor ?B ( NF?B ) signal pathway ... The TNF-a induced iNOS expression in HMC with different level of AR was measured by Real-time PCR and Western blot ... In cultured HMC, TNF-a induces the expression of iNOS , which is attenuated by knockdown AR with siRNA ... Taken together, these data demonstrate the role of AR in regulating iNOS expression induced by TNF-a in cultured HMC, indicating the novel function of AR in glomerulonephritis besides glucose metabolism
Lukacs-Kornek et al., Nat Immunol 2011 : Expression of NOS2 and production of nitric oxide paralleled the activation of T cells and required a tripartite synergism of interferon-?, tumor necrosis factor and direct contact with activated T cells
Escobar et al., Biochem Pharmacol 2012 (Pancreatitis, Acute Necrotizing) : Pentoxifylline markedly reduced the expression of Icam1 and iNos induced by TNF-a in vitro in AR42J cells ... Oxidative stress significantly contributes to the TNF-a induced up-regulation of Icam and iNos in AR42J cells
Cheung et al., J Clin Invest 2011 (Disease Models, Animal...) : Surprisingly, MDL-1 induced NO and TNF-a production required eNOS but not iNOS
Portillo et al., Immunology 2012 (Leishmaniasis, Cutaneous) : In contrast to tumour necrosis factor-a (TNF-a) alone or CD40 stimulation alone, simultaneous stimulation of mouse macrophages through CD40 ligation and TNF-a led to up-regulation of NOS2 and nitric oxide production ... CD40 plus TNF-a up-regulated NOS2 independently of interferon-?, interferon-a/ß and interleukin-1
Gibbs et al., J Biol Chem 2012 : Phorbol ester- and TNF-a dependent activation of the ERK regulated transcription factors Elk1 and NF-?B and expression of the iNOS gene were suppressed by hBVR siRNA ; those activities were rescued by hBVR
Wu et al., Int J Immunopathol Pharmacol 2011 : KMUP-1 inhibited TNF-alpha induced iNOS and U46619 induced PDE-5A and phospho-p38 MAPK in normoxia, confirming its anti-proinflammatory action
Seo et al., Immunopharmacol Immunotoxicol 2012 (Inflammation) : SPEE treatment significantly inhibited the LPS induced production of NO, prostaglandin E ( 2 ), interleukin-6, and tumor necrosis factor-a and inhibited the expression of iNOS and COX-2 via attenuation of NF-?B p65 expression
Kim et al., Mol Cells 2012 (Inflammation) : TNF-a also increased the production of inducible nitric oxide synthase (iNOS) , nitric oxide ( NO ), and intercellular adhesion molecule 1 ( ICAM-1 ) through activation of p38 mitogen activated protein kinase ( MAPK ) from HT-29 cells
Lu et al., Invest Ophthalmol Vis Sci 2012 (Corneal Neovascularization...) : Critical role of TNF-a induced macrophage VEGF and iNOS production in the experimental corneal neovascularization ... Moreover, TNF-a enhanced VEGF and iNOS expression by peritoneal macrophage from WT, but not KO mice
Wang et al., Zhong Xi Yi Jie He Xue Bao 2012 (Adenocarcinoma...) : FYN significantly inhibited TNF-a induced expression of iNOS and COX-2 compared with the control group in A549 cells ( P < 0.01, P < 0.01 ) ... These results suggest that FYN inhibits iNOS and COX-2 activation induced by TNF-a , therefore, it is expected to develop a new strategy to treat lung cancer
Park et al., Laryngoscope 2012 (MAP Kinase Signaling System) : TNF-a significantly increased NO generation and iNOS expression
Yoshioka et al., Mol Reprod Dev 2012 : TNF and IFNG stimulated the relative steady-state amounts of inducible nitric oxide synthase (iNOS) mRNA and iNOS protein expression ( P? < ?0.05 ), whereas P4 inhibited relative steady-state amounts of iNOS mRNA and iNOS protein expression ( P? < ?0.05 )
Goretsky et al., Am J Pathol 2012 (Colitis...) : Therefore, TNF induced iNOS activates a p53 dependent pathway of IEC apoptosis in CUC
Chanthaphavong et al., J Biol Chem 2012 : A Role for cGMP in Inducible Nitric-oxide Synthase (iNOS) induced Tumor Necrosis Factor (TNF) a-converting Enzyme ( TACE/ADAM17 ) Activation, Translocation, and TNF Receptor 1 (TNFR1) Shedding in Hepatocytes
Qu et al., Evidence-based complementary and alternative medicine : eCAM 2012 : In addition, TSAV attenuated the serum TNF-a and IL-6 levels and inhibited the serum iNOS and NO levels
Chung et al., Anesth Analg 2013 (Acute Lung Injury...) : ROS scavenger administration before isoflurane abolished the isoflurane preconditioning effect as well as isoflurane induced inhibition of phosphorylation of inhibitory ?Ba, NF?B p65, iNOS activation , and mRNA expression of TNF-a and IL-6 in acute LPS challenged lungs
Ning et al., PloS one 2013 (Inflammation...) : In addition, NaHS treatment prevented the increase of nitric oxide, Intercellular Adhesion Molecule 1 ( ICAM-1 ) and interleukin (IL)-6 production and inducible nitric oxide synthase activation induced by TNF-a
Song et al., Journal of ginseng research 2012 : Air dried PG leaf extract inhibited TNF-a induced NF-?B transcription activity and NF-?B dependent cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) gene expression more efficiently than the steamed extract ... Furthermore, the ginsenosides Rd and Km inhibited the TNF-a induced expression levels of the COX-2 and iNOS gene in HepG2 cells
Zheng et al., Exp Cell Res 2013 : Tetramethylpyrazine attenuates TNF-a induced iNOS expression in human endothelial cells : Involvement of Syk mediated activation of PI3K-IKK-I?B signaling pathways ... We explored the effect of tetramethylpyrazine ( TMP ), a compound derived from chuanxiong, on tumor necrosis factor (TNF)-a induced iNOS in human umbilical vein endothelial cells ( HUVECs ) and explored the signal pathways involved by using RT-PCR and Western blot ... TMP suppressed TNF-a induced expression of iNOS by inhibiting I?B kinase ( IKK ) phosphorylation, I?B degradation and nuclear factor ?B ( NF-?B ) nuclear translocation, which were required for NO gene transcription
Wilsmann-Theis et al., Allergy 2013 (Dermatitis, Atopic...) : In kinetic experiments, TNF-a , a putative activator of Tip-DC, could not induce NOS2
Park et al., J Physiol Pharmacol 2013 (Inflammation...) : TNF-a increased the expression of COX-2, iNOS and inflammatory cytokines, but GS-HCl significantly attenuated TNF-a induced COX-2 expression
Ryu et al., Planta Med 2013 : Consistent with these observations, echinocystic acid concentration-dependently inhibited lipopolysaccharide induced inducible nitric oxide synthase expression at the protein level and inducible nitric oxide synthase, tumor necrosis factor-a , and interleukin-6 expression at the mRNA level, and inhibited lipopolysaccharide induced iNOS promoter binding activity
Yamada et al., Biochem Biophys Res Commun 1993 : Nitric oxide and nitric oxide synthase mRNA induction in mouse islet cells by interferon-gamma plus tumor necrosis factor-alpha ... Here we show that interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) synergistically induced NO production and inducible NO synthase (iNOS) mRNA expression in mouse islet cells
Radomski et al., Cardiovasc Res 1993 : Stimulation of SGHEC-7 cells with IL-1 beta or TNF alpha ( each at 1-30 ng.ml-1 ) caused them to express the inducible NO synthase , an effect that was prevented by dexamethasone
Koide et al., Hypertension 1994 : These findings suggest that interferon gamma directly induces the expression of the inducible nitric oxide synthase gene, whereas tumor necrosis factor-alpha and interleukin-1 beta induce it, at least in part, via the induction of intermediary protein ( s ), and that transforming growth factor-beta 1 inhibits cytokine induced nitric oxide production by blocking the posttranscriptional synthesis of inducible nitric oxide synthase
Marumo et al., Jpn J Pharmacol 1993 : Dexamethasone inhibits nitric oxide synthase mRNA induction by interleukin-1 alpha and tumor necrosis factor-alpha in vascular smooth muscle cells
Lin et al., Infect Immun 1994 : In this study, we determine whether tumor necrosis factor alpha (TNF-alpha) produced by activated bone marrow derived macrophages ( BMM ) is involved in the induction of the inducible NO synthase gene ( mac-NOS ) for NO-dependent amebicidal activity
Fujihara et al., J Biol Chem 1994 : The role of endogenous tumor necrosis factor alpha (TNF-alpha) and interferon-beta (IFN-beta) in lipopolysaccharide (LPS) induced activation of the inducible nitric-oxide synthase (i-NOS) gene was investigated ... These data thus suggest that endogenous IFN-beta, but not TNF-alpha , produced by LPS stimulated J774 cells specifically contributes , probably in an auto/paracrine fashion, to the activation of the i-NOS gene expression by LPS
Bereta et al., Exp Cell Res 1994 : However, iNOS synthesis requires the presence of both TNF and IFN-gamma, while VCAM-1 can be induced by either cytokine alone
Greenberg et al., Proc Soc Exp Biol Med 1995 : Thus, endogenous TNF-alpha is not required for LPS induced acute phase hypotension or iNOS II activity
Corbett et al., J Exp Med 1995 : Although TNF+LPS induce iNOS expression and inhibit insulin secretion by intact islets, this combination does not induce the expression of iNOS by beta or alpha cells purified by fluorescence activated cell sorting ( Facs ) ... Immunohistochemical localization of iNOS and insulin confirm that TNF+LPS induce the expression of iNOS by islet beta cells, and that a small percentage of noninsulin containing cells also express iNOS
Saura et al., Kidney Int 1995 : PDTC ( 100 microM ) was able to inhibit the iNOS activity induced by LPS and TNF-alpha independently ( 56.8 and 49.9 % inhibition, respectively ), and in combination ( 79.1 % inhibition ) ... PDTC ( 1 to 100 microM ) inhibited LPS + TNF-alpha induced NO synthesis and iNOS mRNA expression in a concentration dependent fashion ( 69 to 86 % inhibition of NO synthesis and 50 to 100 % inhibition of mRNA expression )
Maciejewski et al., J Clin Invest 1995 : iNOS mRNA was also detected by PCR in highly purified CD34+ cells ; TNF-alpha or IFN-gamma increased iNOS expression
Tsuji et al., Am J Pathol 1995 (Granuloma...) : Thus, interleukin-1 alpha and inducible nitric oxide synthase were induced mostly in the cells accumulated around the beads and also in some bronchiolar epithelial cells during the early phase ( 1 to 3 days ), whereas tumor necrosis factor-alpha was induced in the cells around the beads at the later resolution phase ( 3 to 7 days )
Hukkanen et al., Endocrinology 1995 : The present study was performed to investigate the effects of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha) , and interferon-gamma (IFN-gamma) on the expression of inducible NO-synthase (iNOS) and to measure high-output production of NO by primary rat osteoblasts and osteoblastic cell lines ROS 17/2.8, MC3T3-E1 and MG-63
Koide et al., FEBS Lett 1993 : Interferon gamma (IFN gamma) and tumor necrosis factor alpha (TNF alpha) markedly increased iNOS mRNA levels in time- and dose dependent manners ... These results suggest that IFN gamma directly induces the expression of the iNOS gene whereas TNF alpha mainly induces it via the induction of an intermediary protein in cultured VSMC
Xie et al., Am Rev Respir Dis 1993 : Tumor necrosis factor alpha (TNF alpha) increases nitric oxide ( NO ) synthase in vascular endothelium, but it inhibits endothelium dependent relaxation ( EDR ) of vascular smooth muscle
Dunger et al., Diabetes 1996 : Used alone or in combination, TNF and IFN significantly enhanced the activity of inducible nitric oxide synthase as determined by measuring the conversion of 14C labeled arginine to 14C labeled citrulline and nitric oxide
Colasanti et al., Neurosci Lett 1995 : Using reverse transcriptase-polymerase chain reaction ( RT-PCR ) and Southern blot analysis, we also found that LPS/TNF alpha produce an increase of inducible NO synthase (iNOS) mRNA expression
Geng et al., Cancer Res 1996 (Leukemia, Erythroblastic, Acute) : Apoptotic death of human leukemic cells induced by vascular cells expressing nitric oxide synthase in response to gamma-interferon and tumor necrosis factor-alpha
Saura et al., J Biol Chem 1996 : In contrast, sepiapterin, which provides BH4 through the pterin salvage pathway, strongly potentiated IL-1beta/TNF-alpha induced iNOS expression and abrogated the inhibitory effect of DAHP
Kolios et al., Br J Pharmacol 1995 : In marked contrast the presence of TNF-alpha had no effect on IL-1 alpha/IFN-gamma induced iNOS mRNA expression by HT-29 cells
Duval et al., Mol Pharmacol 1996 : NOS2 activity was induced by lipopolysaccharide, IL-1 beta, TNF alpha , and IFN gamma but not by IL-6
Manfield et al., Inflamm Res 1996 : Here we show that in cultured bovine chondrocytes and explants of human osteoarthritic cartilage both nitric oxide synthase and cyclooxygenase activities were induced by the inflammatory mediators, lipopolysaccharide, and interleukin-1 beta or tumor necrosis factor-alpha
Simper et al., Cardiovasc Res 1995 (Shock, Septic) : Experimental and clinical evidence suggests that tumour necrosis factor-alpha (TNF) induces the expression of vascular nitric oxide ( NO ) synthase within hours and that NO released from smooth muscle cells could be involved in the pathogenesis of septic shock
Konur et al., J Immunol 1996 (Carcinoma, Transitional Cell...) : This indicates that IL-1 beta and TNF-alpha play prominent roles in iNOS induction by MO in RT4 tumor cells
Romero et al., Am J Physiol 1996 : Roles of IL-1 and TNF-alpha in endotoxin induced activation of nitric oxide synthase in cultured rat brain cells
Beck et al., FEBS Lett 1996 : TNF-alpha and IL-1beta synergistically stimulate iNOS transcription in rat glomerular mesangial cells
Kurose et al., Hepatology 1996 : Thus, the present study suggests that NO and TNF-alpha released from LPS pretreated Kupffer cells directly inhibit the hepatocyte mitochondrial function in the early phase, and then NO synthesized by TNF-alpha induced hepatocyte iNOS causes lethal hepatocyte injury, characterized by diminished mitochondrial energization and membrane barrier function in the late phase
Zwaveling et al., Crit Care Med 1996 (Neoplasms...) : The hypothesis that in humans, TNF-alpha induces vasodilation and shock through activation of inducible nitric-oxide synthase and subsequent formation of excessive quantities of nitric oxide is not substantiated by our results
Ogura et al., Biochem Biophys Res Commun 1997 (Neuroblastoma) : TNF-alpha mediates inducible nitric oxide synthase expression in human neuroblastoma cell line by cisplatin ... Cisplatin induced tumor necrosis factor-alpha (TNF-alpha) mRNA expression, and an anti-TNF-alpha neutralizing antibody inhibited the induction of iNOS expression by a combination of cisplatin and IFN-gamma in NB-39-nu cells
Le Page et al., Adv Exp Med Biol 1997 : Although the precise mechanism requires further investigation, our results indicate that ADP-ribosylation is a crucial step restricted to the signalling pathway which leads to iNOS mRNA induction, as well as TNF and MHC class II induction during macrophage activation
Xu et al., Biochem Biophys Res Commun 1997 : In an attempt to modulate TNF-alpha+IFN-gamma induced expression of iNOS in MCECs, we designed a double-strand hairpin ( hp ) oligonucleotide carrying the NF-kappaB motif ... The present study confirms the role of transcription factor NF-kappaB in iNOS expression induced by TNF-alpha+IFN-gamma in MCECs
Obregón et al., Eur J Neurosci 1997 (Brain Neoplasms...) : The isoform of the nitric oxide synthase induced in human neuroblastoma cells by TNF-alpha treatment was identified enzymatically as isoform II by Western blotting and by the polymerase chain reaction
Oda et al., J Neurochem 1997 : The inhibitory effect of ET on TNF-alpha/IL-1beta stimulated iNOS expression appears to be mediated by ET ( B ) receptors, because ( 1 ) both ET-1 and ET-3 inhibited the effects of TNF-alpha/IL-1beta on iNOS expression and nitrite accumulation, ( 2 ) a selective ET ( B ) receptor agonist, Suc- [ Glu ( 9 ), Ala ( 11,15 ) ] -ET-1 ( 8-21 ) ( IRL1620 ), decreased the effects of TNF-alpha/IL-1beta, and ( 3 ) a selective ET ( B ) receptor antagonist, N-cis-2,6-dimethylpiperidinocarbonyl-L-gamma-methylleucyl-D- 1-methoxycarbonyltryptophanyl-D-norleucine, abolished the inhibitory effects of ETs and IRL1620 ... Furthermore, cyclic AMP elevating agents ( dibutyryl cyclic AMP and forskolin ) inhibited TNF-alpha/IL-1beta induced and LPS induced iNOS expression and nitrite accumulation ... These findings suggest that ETs can decrease TNF-alpha/IL-1beta induced and LPS induced iNOS expression via ET ( B ) receptors and that cyclic AMP may be involved in this process
Gansauge et al., FEBS Lett 1997 : Induction of inducible nitric oxide synthase by tumor necrosis factor-alpha , interferon-gamma and interleukin-1beta inhibited cell proliferation and led to a G1 arrest
Fukumoto et al., Parasite Immunol 1997 : The suppression of inducible mRNA levels in macrophages cultured for 24 h with ES products varied with the nature of the stimuli ; IFN gamma/ LPS induced iNOS mRNA levels were effected less than were iNOS mRNA levels induced by IFN gamma/IL-2 or IFN gamma/ TNF alpha
Kuo et al., Mol Pharmacol 1997 : Interestingly, antioxidants such as N-acetylcysteine (NAC), ascorbic acid, or alpha-tocopherol fail to inhibit TNF-alpha/LPS/ethanol induced increase in iNOS protein
Li et al., Can J Anaesth 1997 : In rat alveolar macrophages, ( i ) ketamine ( 1 to 100 microM ) caused a dose dependent suppression of the production of nitrite and TNF-alpha induced by LPS and ( ii ) ketamine ( 100 microM ) inhibited the activity ( 46.5 +/- 4.8 %, P < 0.05 ) and protein expression ( 35 +/- 11 %, P < 0.05 ) of iNOS in response to LPS
Kobuchi et al., Biochem Mol Biol Int 1997 : Tumor necrosis factor-alpha and interleukin-1 beta are involved in the induction of iNOS gene as well as the immune system
Birks et al., Coron Artery Dis 1997 (Heart Failure) : TNF-alpha and IL-6 may be produced in heart failure and may induce inducible NO synthase , resulting in NO production, which acts as a negative inotrope
Da Silva et al., J Biol Chem 1997 : Treatment of mouse astrocyte cultures with combined interleukin (IL)-1alpha and tumor necrosis factor (TNF)-alpha induced expression of inducible nitric-oxide synthase (iNOS), resulting in sustained release of large amounts of nitric oxide, whereas TNF-alpha and IL-1alpha individually were unable to induce iNOS expression in astrocytes ... The p38 ( MAPK ) pathway is specifically involved in TNF-alpha/IL-1alpha induced iNOS expression, since iNOS protein and nitric oxide release in the presence of a specific inhibitor of p38 ( MAPK ), 4- ( 4-fluorophenyl ) -2-2- ( 4-hydroxyphenyl ) -5- ( 4-pyridyl ) -imidazole ( FHPI ), were dramatically diminished ... These data suggest that TNF-alpha/IL-1alpha induced iNOS expression depends on a yet undetermined second pathway in addition to p38 ( MAPK )
Uchida et al., Eur J Pharmacol 1997 : A reverse transcription-polymerase chain reaction ( RT-PCR ) assay revealed that TNF-alpha caused a concentration- and time dependent expression of inducible NO synthase in brown adipocytes
Kinugawa et al., Circ Res 1997 : Forskolin, interleukin (IL)-6, tumor necrosis factor (TNF)-alpha , or interferon (IFN)-gamma enhanced the LPS induced iNOS expression ... Combined stimulation of IL-6 and TNF-alpha also induced iNOS
Müller-Werdan et al., J Mol Cell Cardiol 1997 : However, this low TNF alpha-concentration does not suffice to induce iNOS - documented by reverse transcriptase polymerase chain reaction - or enhance nitrite concentrations in the cell culture supernatants as a measure of cellular NO production, neither in the presence nor absence of dexamethasone ( 0.1 micro M )
Worrall et al., Am J Physiol 1997 : Our results showed 1 ) TNF-alpha reversibly increased albumin permeation in the systemic vasculature ( e.g., lung, liver, brain, etc. ) ; 2 ) TNF-alpha did not affect hemodynamics or blood flow or cause significant tissue injury ; 3 ) pulmonary vascular barrier dysfunction was associated with increased lung water content and impaired oxygenation ; 4 ) TNF-alpha caused inducible nitric oxide synthase (iNOS) mRNA expression in the lung and increased in vivo NO production ; 5 ) selective inhibition of iNOS with aminoguanidine prevented TNF-alpha induced lung and liver vascular barrier dysfunction ; 6 ) aminoguanidine prevented increased tissue water content in TNF-alpha treated lungs and improved oxygenation ; and 7 ) nonselective inhibition of NOS with NG-monomethly-L-arginine increased vascular permeation in control lungs and caused severe lung injury in TNF-alpha treated animals
Kuemmerle et al., Am J Physiol 1998 : Synergistic regulation of NOS II expression by IL-1 beta and TNF-alpha in cultured rat colonic smooth muscle cells ... After 48 h, NOS II activity, measured as L- [ 3H ] citrulline production, was increased 24.3 +/- 6.9 pmol.min-1.mg protein-1 by 1 nM IL-1 beta and 3.2 +/- 1.1 pmol.min-1.mg protein-1 by 1 nM TNF-alpha , and increased synergistically by a combination of the two ( 51.8 +/- 7.3 pmol.min-1.mg protein-1 ) ... The increase in NOS II activity induced by IL-1 beta and TNF-alpha was inhibited 73-86 % by transforming growth factor-beta 1 ( TFG-beta 1 )
Brian et al., Stroke 1998 : TNFalpha alone or in combination with endotoxin or other cytokines can cause expression of inducible nitric oxide (NO) synthase ... The present study indicates that TNFalpha causes cerebral vasodilatation and expression of inducible NO synthase in perivascular and arachnoid cells
Oda et al., Eur J Pharmacol 1997 : These results suggest that the increase in inducible NO synthase expression by endothelin was due to the elevated TNF-alpha production via protein kinase C
Jobin et al., J Immunol 1998 : Induction of inducible nitric-oxide synthase (iNOS) , IL-1beta, and IL-8 genes by IL-1beta, TNF-alpha , or PMA was blocked in Ad5 IkappaB infected cells but not in Ad5 LacZ controls as assayed by RT-PCR and ELISA
Goff et al., J Interferon Cytokine Res 1998 : We previously demonstrated that iNOS can be induced in cultured bovine monocytes in response to IFN-gamma and TNF-alpha but lose this capability in a short period of time
Li et al., Can J Anaesth 1998 : In rats alveolar macrophages, ( i ) dantrolene ( 1 to 100 microM ) caused a dose dependent suppression of the production of nitrite and TNF-alpha induced by LPS ( 1 microgram.ml-1 ) plus IFN-gamma ( 100 u.ml-1 ) and ( ii ) dantrolene ( 100 microM ) inhibited the activity ( by 37 +/- 5 %, P < 0.01 ) and protein expression ( by 39 +/- 12 %, P < 0.01 ) of iNOS in response to LPS plus IFN-gamma
Brodie et al., FEBS Lett 1998 : Treatment of the cells with the A2A receptor agonist CGS 21680 inhibited both NO production and iNOS expression induced by stimulation with either LPS/IFN-gamma or TNF-alpha/IL-1beta , whereas the A1 and A3 receptor agonists, CPA and Cl-IB-MECA, respectively, did not have significant inhibitory effects
Arnush et al., J Clin Invest 1998 : Inhibition of iNOS activity by aminoguanidine also attenuates TNF + LPS + IFN-gamma induced inhibition of insulin secretion by human islets
Hoffmann et al., Int Arch Allergy Immunol 1998 : Single stimulation of smooth muscle cells with either neopterin or tumor necrosis factor-alpha caused inducible nitric oxide synthase gene expression and nitric oxide production
Martins et al., Immunol Lett 1998 (Chagas Disease) : Production of gamma-interferon ( IFN-gamma ) and tumor necrosis factor-alpha (TNF-alpha) in Trypanosoma cruzi infected mice results in the activation of inducible nitric oxide synthase (iNOS) and in elevated nitric oxide ( NO ) synthesis, which is important for the macrophage trypanocidal activity
Yang et al., Hepatology 1998 (Liver Diseases...) : Hepatocellular, but not bile duct, injury occurs during experimental pancreatitis that is associated with hepatic TNF-, IL-1beta, and iNOS mRNA gene induction , as well as TNF- protein and nitrite production
Hoffmann et al., Eur J Pharmacol 1998 : We show that G-CSF suppresses interferon-gamma/tumor necrosis factor-alpha (TNF-alpha) induced inducible nitric oxide synthase gene expression detected as inducible nitric oxide synthase cDNA ( cDNA concentration was 633 +/- 38 amol/microg total RNA following 24 h incubation with 100 U/ml interferon-gamma/500 U/ml TNF-alpha, and 440 +/- 14 amol/microg total RNA following 24 h incubation with 250 U/ml G-CSF +/- 100 U/ml interferon-gamma/500 U/ml TNF-alpha, respectively )
Yanagisawa et al., Toxicol Appl Pharmacol 1998 (Acute Kidney Injury...) : To elucidate the mechanisms underlying the development of HgCl2 induced acute renal failure ( ARF ), we examined the expression of endothelin (ET)-1, endothelial ( e ) nitric oxide synthase (NOS) and inducible ( i) NOS , and a role of angiotensin II (ANG II) and tumor necrosis factor (TNF) in glomeruli and cortices from rats at 20 h after exposure of HgCl2. Prepro-ET-1 and iNOS mRNA were significantly increased in glomeruli and cortices from rats with HgCl2 induced ARF ... In HgCl2 induced ARF, the gene expression of ET-1 or iNOS is at least in part up-regulated at the transcription level by endogenous ANG II or TNF
Ookawauchi et al., J Hepatol 1998 : The effect of tumor necrosis factor-alpha and interferon-gamma on L-arginine transport, mRNA expression of cationic amino acid transporter and inducible nitric oxide synthase , and nitric oxide production of stellate cells was assessed ... In transformed hepatic stellate cells, tumor necrosis factor-alpha and interferon-gamma have a crucial role in nitric oxide production, and extracellular L-arginine transport and inducible nitric oxide synthase expression are regulated in a differential cytokine-specific manner
Chan et al., Biochem Biophys Res Commun 1998 : Potential role of the JNK/SAPK signal transduction pathway in the induction of iNOS by TNF-alpha