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LPA — VEGFA

Text-mined interactions from Literome

Fang et al., Ann N Y Acad Sci 2000 (Ascites...) : LPA increases cell proliferation, cell survival, resistance to cisplatin, cell shrinkage, and production of vascular endothelial growth factor , urokinase plasminogen activator, and LPA itself in ovarian cancer cells, but not in normal ovarian surface epithelial cells
Hu et al., J Natl Cancer Inst 2001 (Ovarian Neoplasms) : LPA treatment increased steady-state VEGF messenger RNA ( mRNA ) levels in OVCAR-3 cells in a time- and dose dependent fashion and stimulated VEGF promoter activity without prolonging mRNA half-life in these cells, but LPA had little effect on IOSE-29 cells
Shida et al., Cancer Res 2003 (Colonic Neoplasms...) : Furthermore, LPA stimulated DLD1 cell adhesion to collagen type I ( 2.0-fold increase at 10 microM ) and also stimulated the secretion of both vascular endothelial growth factor ( 1.4-fold increase at 20 microM ) and interleukin 8 ( 19-fold increase at 20 microM ) by ELISA
Tanyi et al., Clin Cancer Res 2003 (Ovarian Neoplasms) : LPA stimulates cell proliferation, cell survival, resistance to cisplatin, production and activation of proteases, invasiveness and production of the neovascularizing factors, vascular endothelial growth factor , and interleukin 8
Sako et al., J Surg Res 2006 (Ascites...) : Lysophosphatidic acid (LPA) induced vascular endothelial growth factor ( VEGF ) by mesothelial cells and quantification of host derived VEGF in malignant ascites ... The effect of LPA on the production of VEGF was evaluated by ELISA and northern blotting
Lee et al., Clin Cancer Res 2006 (Neoplasm Invasiveness...) : Specific HIF-1alpha small interfering RNA was transfected to various cancer cells to determine the role of HIF-1alpha in LPA induced VEGF expression ... LPA induced expressions of VEGF and HIF-1alpha in OVCAR-3, CAOV-3, PC-3, and SK-Hep1 cells but not in SKOV-3 and Hep-3B cells ... In OVCAR-3 and PC-3 cells, the phosphoinositide 3-kinase/Akt/mammalian target of rapamycin/p70S6K and p42/p44 mitogen activated protein kinase pathways were required for LPA induced HIF-1alpha and VEGF expressions, whereas only the phosphoinositide 3-kinase/mammalian target of rapamycin/p70S6K pathway was important in SK-Hep1 cells ... Our results show for the first time that LPA induces VEGF via HIF-1alpha activation and reveal a critical role of HIF-1alpha in LPA induced cancer cell proliferation and angiogenesis
Bermudez et al., Gynecol Oncol 2007 (Ovarian Neoplasms) : We report that : ( 1 ) both VEGF and LPA upregulate telomerase activity ; ( 2 ) LPA induction of telomerase activity is VEGF dependent ; ( 3 ) VEGF and LPA induction of telomerase activity is ERK 1/2 dependent ; and ( 4 ) Sp1 binding sites within the proximal 976- to 378-bp regions of the hTERT promoter are essential for VEGF- and LPA induced hTERT promoter activity
Park et al., Cancer Lett 2007 (Ovarian Neoplasms) : Hypoxia enhances LPA induced HIF-1alpha and VEGF expression : their inhibition by resveratrol ... In the present study, we investigated the effects of LPA and hypoxia on HIF-1alpha and VEGF expression, as well as the effect of resveratrol on LPA and hypoxia induced HIF-1alpha and VEGF expression and human ovarian cancer cell migration
Hu et al., Cell Signal 2008 (Burkitt Lymphoma...) : Lysophosphatidic acid (LPA) induces the expression of VEGF leading to protection against apoptosis in B-cell derived malignancies ... Herein, we demonstrate that lysophosphatidic acid (LPA) induces mRNA expression of VEGF in the multiple myeloma cell line, U266, the Burkitt 's lymphoma cell line, BJAB, and the chronic lymphocytic leukemia ( CLL ) -like cell line, I-83 ... The increase in VEGF expression by LPA is mediated by the activation of c-Jun N-terminal Kinase (JNK) and transcription factor NFkappaB since blocking JNK or NFkappaB activation inhibited LPA induced VEGF expression ... Taken together, this indicates that LPA contributes to VEGF production in B cell malignancies leading to cell survival
Chen et al., Endocrinology 2008 : The effects of LPA on the expression of vascular endothelial growth factor , IL-6, and IL-8 were examined
Lin et al., Cell Mol Life Sci 2008 : Previous studies have shown that LPA enhances vascular endothelial growth factor-A ( VEGF-A ) expression in cancer cells and promotes angiogenesis process
Song et al., Clin Cancer Res 2009 (Neovascularization, Pathologic...) : ELISA, Northern blotting, immunoblotting, quantitative PCR, and promoter reporter analysis in combination with small interfering RNA and pharmacologic inhibitors were used to examine LPA induced VEGF expression and the underlying mechanisms ... Blockade of Rho, ROCK, or c-Myc reduced LPA dependent VEGF production and promoter activation, suggesting that the G12/13-Rho-ROCK-c-Myc cascade partially contributes to VEGF induction by LPA ... The silencing of Sp-1 expression with small interfering RNA or inhibition of Sp-1 with pharmacologic inhibitors blocked VEGF production induced by LPA ... LPA stimulates hypoxia-inducible factor-1 independent VEGF expression to promote tumor angiogenesis through activation of the c-Myc and Sp-1 transcription factors
Liu et al., Stem Cells Dev 2009 (Myocardial Ischemia) : Moreover, transplantation of LPA treated MSCs enhanced capillary density determined by immunostaining for platelet endothelial cell adhesion molecule ( PECAM)-1, and it is also found that LPA enhanced vascular endothelial growth factor ( VEGF ) release from MSCs under hypoxia/SD in vitro
Wang et al., Gynecol Oncol 2009 (Neoplasm Invasiveness...) : We also tested the effect of LPA on VEGF and VEGFR-2 expression and examined whether blocking VEGFR-2 by RNA interference ( RNAi ) affects LPA induced EOC invasion ... In addition, LPA , at 20 muM, significantly induced the expression of VEGF ( 121 ), VEGF ( 165 ), and VEGFR-2 in SKOV3 and DOV13 cells ( P < 0.05 )
Kumar et al., Leukemia & lymphoma 2009 (Leukemia, Lymphocytic, Chronic, B-Cell) : In contrast, plasma levels of VEGF are elevated in patients with CLL compared to healthy controls and LPA treatment induced VEGF secretion in CLL cells
Boucharaba et al., Oncol Res 2009 (Breast Neoplasms) : S1P, but not LPA , controlled the expression of VEGF-A by breast cancer cells, while LPA, but not S1P, controlled the expression of GM-CSF, Gro-alpha, MCP-1, and IL-6
Jeon et al., Exp Mol Med 2010 (Ovarian Neoplasms) : LPA activated RhoA in hASCs and pretreatment with the Rho kinase inhibitor Y27632 completely abrogated the LPA induced expression of alpha-SMA, SDF-1, and VEGF in hASCs ... LPA induced VEGF secretion was inhibited by LY294002, whereas LPA induced SDF-1 secretion was markedly attenuated by U0126, U73122, and LY294002
Ptaszynska et al., Mol Cancer Res 2010 (Neovascularization, Pathologic) : VEGF stimulated expression of ATX and LPA1 , a receptor for the ATX enzymatic product lysophosphatidic acid (LPA), in human umbilical vein endothelial cells ... We propose that VEGF stimulates LPA production by inducing ATX expression ... VEGF also increases LPA1 signaling, which in turn increases Akt2 expression
Dutta et al., Gynecol Oncol 2011 (Neoplasm Invasiveness...) : The NF-?B pathway mediates lysophosphatidic acid (LPA) induced VEGF signaling and cell invasion in epithelial ovarian cancer ( EOC ) ... However, the mechanism by which LPA regulates VEGF and VEGFR-2 expression remains to be elucidated ... In the present study, we systematically examined the signal transduction pathways activated by LPA and further evaluated whether LPA 's effect on VEGF-VEGFR-2 signaling and EOC invasion was mediated by the activation of NF-?B pathway ... In addition, treatment with an NF-?B pathway activation inhibitor, at 10 µM, significantly decreased LPA induced VEGF ( 121 ), VEGF ( 165 ) and VEGFR-2 mRNA expression and VEGF secretion, as well as LPA induced SKOV3 invasion ( p < 0.05 ) ... When combined with an EGFR inhibitor, NF-?B pathway inhibition exhibited a significantly stronger effect than used alone ( p < 0.05 ) on reducing LPA induced VEGF secretion and cell invasion ... These results suggest that the NF-?B pathway plays an important role in LPA induced VEGF signaling and EOC invasion and targeting this pathway may reveal potential therapeutic options for metastatic EOC
Wei et al., Biochim Biophys Acta 2013 : In this study, we examined the pharmacological and molecular regulation of VEGF secretion induced by LPA in rat MSCs ... We showed that LPA stimulated VEGF secretion in MSCs but not in cardiomyocytes or cardiac fibroblasts ... LPA induced VEGF secretion occurred at the post-transcriptional levels and was mediated through the classical ER/Golgi dependent protein secretory route ... Inhibition of ORP150 upregulation by siRNA knockdown attenuated LPA induced VEGF secretion ... On the other hand, diazoxide, an activator of KATP channel, markedly inhibited LPA induced ORP150 expression and VEGF secretion ... Furthermore, inhibition of two major subtypes of LPA receptors by Ki16425 and specific siRNA for LPA receptors prevented LPA induced VEGF secretion and ORP150 expression ... Lastly, inhibition of Gi protein that couples with LPA receptors by PTX and siRNA knockdown had no effect on LPA induced VEGF secretion ... Taken together, our findings demonstrate that LPA promotes VEGF secretion at the post-translation level by up-regulating ORP150 expression ... Both LPA1 and LPA3 are involved in the LPA induced VEGF secretion that is independent of Gi protein coupling but associated with the inactivation of KATP channels and inhibition of Na ( + ) /K ( + ) -ATPase activity
Wu et al., Biochem Biophys Res Commun 2013 (Neoplasm Metastasis...) : In this study, we first confirmed the ability of LPA to induce VEGF-A expression in PC-3 cells and then validated that LPA induced VEGF-A expression was regulated by HIF-1a and ARNT through phosphatidylinositol 3-kinase activation ... Since ARNT is a common dimerization partner of AHR and HIF-1a, we hypothesized that AHR might suppress LPA induced VEGF-A expression in PC-3 cells by competing with HIF-1a for ARNT ... In conclusion, our results suggested that AHR activation may inhibit LPA induced VEGF-A expression in PC-3 cells by attenuating HIF-1a signaling, and subsequently, suppressing angiogenesis and metastasis of PC