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PAF1 — VEGFA

Text-mined interactions from Literome

Montrucchio et al., Arterioscler Thromb Vasc Biol 2000 : The purpose of the present study was to evaluate whether the synthesis of PAF induced by vascular endothelial growth factor ( VEGF ) might affect endothelial cell motility, microvascular permeability, and angiogenesis ... The neoangiogenesis and synthesis of PAF induced by VEGF were studied in vivo in a murine Matrigel model ... In vitro, it was found that VEGF induced the synthesis of PAF by HUVECs in a dose- and time dependent manner ... These results suggest that the synthesis of PAF induced by VEGF enhances endothelial cell migration and contributes to the angiogenic effect of VEGF in the in vivo Matrigel model
Bernatchez et al., Br J Pharmacol 2001 : Since the phospholipid remodelling pathway enzymes ( CoA independent transacylase, CoA-IT ; phospholipase A2, PLA2 ; and lyso-PAF acetyltransferase, lyso-PAF-AT ) may participate in PAF synthesis, we assessed their contribution to VEGF induced PAF synthesis in bovine aortic EC ( BAEC ) and human umbilical vein EC ( HUVEC ) ... 3. A pretreatment with a CoA-IT and lyso-PAF-AT inhibitor ( Sanguinarin ; 500 nM ) blocked VEGF induced PAF synthesis by 95 %, a specific CoA-IT inhibitor ( SKF45905 ; 10 - 50 microM ) was without effect, confirming the crucial role of the PLA2 and lyso-PAF-AT
Bernatchez et al., Br J Pharmacol 2001 : Regulation of VEGF induced endothelial cell PAF synthesis : role of p42/44 MAPK, p38 MAPK and PI3K pathways ... However, the intracellular events that regulate EC PAF synthesis upon Flk-1 stimulation by VEGF remain to be elucidated ... 2. Using specific inhibitors and Western blot analysis, we herein report that in bovine aortic endothelial cells ( BAEC ), VEGF induces the synthesis of PAF through the cascade activation of Flk-1 receptor, phospholipase Cgamma ( PLCgamma ), protein kinase C ( PKC ) and p42/44 mitogen activated protein kinases ( MAPK ) ... 3. Moreover, we demonstrate that VEGF mediated PAF synthesis requires the activation of p38 MAPK, likely by directing the conversion of lyso-PAF to PAF ... 4. Interestingly, we observed that VEGF also promoted the activation of the phosphatidyl inositol-3-phosphate kinase ( PI3K ) pathway, and that its blockade potentiated PAF synthesis following a VEGF treatment ... 5. Taken together, these results allow a better understanding of the intracellular events activated upon EC stimulation by VEGF, and shed a new light on the mechanisms by which VEGF induces PAF synthesis
Bernatchez et al., J Cell Biochem 2002 : To assess the contribution of NRP-1 to VEGF-A mediated EC proliferation, migration, and PAF synthesis, we used porcine aortic EC ( PAEC ) recombinantly expressing Flt-1, NRP-1, KDR or KDR and NRP-1 ... In contrast, stimulation of PAEC-Flt-1 and PAEC-NRP-1 with VEGF-A or VEGF-C did not induce proliferation, migration, or PAF synthesis
Yoshida et al., Brain Res 2002 (Hypoxia, Brain...) : PAF increased the expression of VEGF in astrocytes in time- and dose dependent manners ... To examine whether platelet activating factor (PAF) induces the expression of VEGF in human astrocytes, we stimulated cultured normal astrocytes with PAF and performed semi-quantitative reverse transcription-polymerase chain reaction ( RT-PCR ) or real-time quantitative PCR for VEGF mRNA and enzyme linked immunosorbent assay for VEGF protein ... After 24-h stimulation, 10 nM PAF increased the levels of VEGF protein in astrocyte conditioned medium by 1.3-fold ... When the cells were subjected to hypoxia, the PAF induced production of VEGF was enhanced by 6.7-fold as compared to the unstimulated cells incubated under normoxia ... Dexamethasone was found to inhibit the enhanced VEGF production in response to the stimulation with PAF under hypoxia ... We conclude that PAF induces VEGF gene expression in human astrocytes, and the PAF induced increase in the expression of VEGF may modulate nervous tissue injury due to hypoxia
Gélinas et al., Br J Pharmacol 2002 : In addition, as VEGF induces the synthesis of NO and platelet activating factor (PAF) , we wanted to assess if the induction of PAF and NO is contributing to the synthesis of each other
Ma et al., Invest Ophthalmol Vis Sci 2004 (Corneal Neovascularization) : Platelet activating factor (PAF) induces corneal neovascularization and upregulates VEGF expression in endothelial cells
Marchand et al., J Cell Biochem 2006 : Role of MSK1 in the signaling pathway leading to VEGF mediated PAF synthesis in endothelial cells ... We previously reported that VEGF mediated PAF synthesis involves the activation of VEGF receptor-2/Neuropilin-1 complex, which is leading to the activation of p38 and p42/44 mitogen activated protein kinases ( MAPKs ) and group V secretory phospholipase A ( 2 ) ( sPLA ( 2 ) -V ) ... These results demonstrate the essential role of the C-terminal domain of MSK1 for its constitutive interaction with sPLA ( 2 ) -V, which appears essential to support VEGF mediated PAF synthesis
Ko et al., FEBS Lett 2006 : We investigated the role of p53 in nuclear factor (NF)-kappaB dependent , platelet activating factor (PAF) induced vascular endothelial growth factor ( VEGF ) expression ... PAF induced increase in VEGF expression was correlated with decreased p53 activity
Brkovic et al., J Cell Biochem 2007 : We previously reported that vascular endothelial growth factor ( VEGF ) increases vascular permeability through the synthesis of endothelial platelet activating factor (PAF) , while others reported the contribution of nitric oxide ( NO )
He et al., Curr Eye Res 2009 : PAF also increased the VEGF level in RPE cells
He et al., Curr Eye Res 2010 (Corneal Neovascularization) : PAF induced a significant increase in VEGF expression and a decrease in TSP-1 expression
Rechka et al., Can J Physiol Pharmacol 2010 : We previously reported that VEGF mediated PAF synthesis involves the activation of the homodimeric tyrosine kinase receptor VEGFR-2/R-2, leading to the recruitment of p38 and p42/p44 mitogen activated protein kinases ( MAPKs ) and activation of secreted group V phospholipase A2 ( sPLA2-V ) ... In BAECs transfected with pcDNA vectors encoding mutated Y801F, Y1059F, Y1175F, or Y1214F VEGFR-2 cDNA, we observed a marked reduction of VEGF mediated PAF synthesis by 38 %, 46 %, 69 %, and 31 %, respectively, compared with BAECs transfected with pcDNA vector encoding VEGFR-2 cDNA ... Our data provide a novel insight as to the mechanisms by which VEGF promotes PAF synthesis
Kim et al., Cell Physiol Biochem 2011 : It has been previously reported that platelet activating factor (PAF) induces the expression of vascular endothelial growth factor ( VEGF ) via the downregulation of p53 activity ... In this study, we attempted to characterize the mechanism by which p53 activity negatively regulates PAF induced VEGF expression ... PAF increased luciferase activity as well as VEGF mRNA expression in human non-small cell lung cancer cell line H1299 transfected with VEGF luciferase reporter plasmid ( VEGF-Luc ) ... Cotransfection of the cells with wt p53, but not mutant p53, effected a blockage of PAF induced VEGF mRNA expression
Nauck et al., Am J Respir Cell Mol Biol 1997 : To determine whether PAF or PDGF induce VEGF gene expression in primary cultures of human pulmonary fibroblasts and pulmonary vascular smooth-muscle cells ( VSMCs ), we performed Northern-blot analysis and enzyme linked immunosorbent assays ( EIA )