Gene interactions and pathways from curated databases and text-mining

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NFKB1 — TNF

Pathways - manually collected, often from reviews:

Text-mined interactions from Literome

Sen et al., Biochem Biophys Res Commun 1998 : At 150 microM LA-Plus, but not LA, inhibited TNFalpha induced NF-kappaB activation
Umansky et al., Eur J Immunol 1998 (Inflammation) : High doses of NO impaired the TNF-alpha induced DNA binding activity of NF-kappaB
Huang et al., BMC systems biology 2008 : We have used this technique to quantitatively characterize activation of the transcription factor NF-kappaB by the cytokine TNF-alpha
Hu et al., J Immunol 1999 (Leukemia, Erythroblastic, Acute...) : TNF-alpha rapidly induced marked activation of nuclear transcription factor NF-kappa B in all 4 cell lines
Ros et al., Hepatology 2001 (Carcinoma, Hepatocellular...) : Induction of Mdr1b expression by tumor necrosis factor-alpha in rat liver cells is independent of p53 but requires NF-kappaB signaling ... Because p53 is up-regulated by TNF-alpha in an NF-kappaB dependent manner and the Mdr1b promoter contains a p53 binding site, we used liver cells expressing a dominant negative p53 to show that TNF-alpha up-regulation of Mdr1b is independent of functional p53 ... In conclusion our results show that activation of the rat Mdr1b gene by TNF-alpha is a result of NF-kappaB signaling and independent of p53
Shen et al., FASEB J 2006 : Recently, some important discoveries have underscored the critical role of ROS in TNFalpha signaling, notably in TNFalpha mediated activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase ( c-Jun NH2-terminal kinase, JNK ), as well as in cell death ( apoptotic and necrotic ) pathways ... Here we attempt to review the existing knowledge on the involvement of ROS in death receptor signaling using TNFalpha-TNFR1 as the model system, specifically addressing the involvement of intracellular ROS in TNFalpha induced cell death and in TNFalpha induced activation of NF-kappaB and JNK and their crosstalk
Verstrepen et al., J Cell Mol Med 2008 (Sepsis) : This revealed that the LPS- and TNF-inducible expression of ABIN-3 is dependent on the binding of NF-kappaB to a specific B site in the ABIN-3 promoter
Horie et al., Microbes Infect 2007 (HIV Infections) : Previous studies indicated that NF-kappaB activation by tumor necrosis factor (TNF) receptor family, which activates TNF receptor associated factor (TRAF) , induces HIV-1 expression
Hellerbrand et al., Hepatology 1998 (Liver Cirrhosis, Experimental) : Proteasome inhibitors, which block the degradation of IkappaB, and the Ad5IkappaB, which provides an exogenous nondegradable IkappaB, block the stimulation of NFkappaB activity by TNFalpha and IL-1beta in activated HSCs
D'Alimonte et al., J Immunol 2007 : Moreover, guanosine abrogated IFN-gamma induced phosphorylation on Ser ( 727 ) and translocation of STAT-1alpha to the nucleus as well as TNF-alpha-/Abeta induced IkappaBalpha and NF-kappaB p65/RelA subunit phosphorylation, thus inhibiting NF-kappaB induced nuclear translocation ... Guanosine effects were mediated by an increased phosphorylation of Akt, a PI3K downstream effector, as well as of ERK1/2 and p38 in the MAPK system, because culture pretreatment with selective ERK1/2, p38 MAPK, and PI3K antagonists ( U0126, SB203580, or LY294002, respectively ) counteracted guanosine inhibition on IFN-gamma/TNF-alpha induced CD40 expression and function as well as on STAT-1alpha or NF-kappaB nuclear translocation
Pyatt et al., Cell Biol Toxicol 2000 : We have previously demonstrated that 1 micromol/L HQ inhibits TNF induced activation of NFkappaB in CD4+ T cells, resulting in decreased IL-2 production
Li et al., Acta Pharmacol Sin 2006 (Pulmonary Disease, Chronic Obstructive) : Similarly, NAC inhibited the activation of NF-kB induced by TNF- alpha , but had no effect on the activation of NF-kappaB induced by IL-1 ... PDTC significantly inhibited the activation of NF-kappa B induced by TNF- alpha and IL-1
Hatano , J Gastroenterol Hepatol 2007 (Liver Diseases) : The TNF-alpha and Jo2 induced iNOS through NF-kappaB
Tolosa et al., Cell Signal 2005 : This cytokine induced down-regulation of the subtype 2 of TNF-alpha receptors ( TNF-R2 ), enhanced TNF-alpha induced NF-kappaB translocation to the nucleus and binding to DNA and increased the immunoreactivity of the protein c-IAP1, a member of the inhibitor of apoptosis (IAP) gene family whose synthesis is stimulated by NF-kappaB at the transcriptional level
Yamazaki et al., J Biol Chem 2005 : We have recently identified an inducible nuclear factor-kappaB (NF-kappaB) regulator, IkappaB-zeta, which is induced by microbial ligands for Toll-like receptors such as lipopolysaccharide and the proinflammatory cytokine interleukin (IL)-1beta but not by tumor necrosis factor (TNF)-alpha
Chen et al., Life Sci 1999 : In the present study, NF-kappaB was activated by TNF-alpha in rat aortic smooth muscle cells as demonstrated by electrophoretic mobility shift assay ... The activity of NF-kappaB induced by TNF-alpha was blocked by calpain inhibitor I, a potent NF-kappaB inhibitor
Hong et al., BMC molecular biology 2007 : Functionally, transiently overexpressed Zfra sequestered NF-kappaB ( p65 ), WOX1, p53 and phospho-ERK ( extracellular signal activated kinase ) in the cytoplasm, and TNF or UV light could not effectively induce nuclear translocation of these proteins
Rothe et al., Science 1995 : TRAF2 mediated activation of NF-kappa B by TNF receptor 2 and CD40
Takada et al., J Immunol 2003 : BA suppressed NF-kappaB activation induced by TNF , PMA, cigarette smoke, okadaic acid, IL-1, and H ( 2 ) O ( 2 )
Boland et al., J Biol Chem 1998 : Furthermore, the effect of TNF on NFkappaB activation was rapid, whereas C2-ceramide required an optimal treatment time of 1 h. Interestingly, TNF was found to increase ceramide in cells but only after a 1-h contact time
Hess et al., Bone 2009 : We conclude that TNFalpha mediated NF-kappaB activation, although not absolutely required for BMP-2 expression and matrix mineralization nevertheless supports osteogenic differentiation and matrix mineralization by increasing BMP-2 expression
Wong et al., Int Immunopharmacol 2006 : However, the expression of VCAM-1 on BEAS-2B cells was only up-regulated by TNF-alpha induced NF-kappaB activity
Xu et al., Am J Physiol 1998 : ActD did not affect TNF-alpha induced hepatocyte NF-kappaB activation but decreased NF-kappaB dependent gene expression
Russo et al., Am J Physiol Cell Physiol 2002 : Differential requirement for NF-kappaB inducing kinase in the induction of NF-kappaB by IL-1beta, TNF-alpha , and Fas ... Interleukin (IL)-1beta- and tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activation and kappaB dependent gene expression are inhibited in HeLa cells but not in Ad5dnNIK infected HT-29 cells
Chakrabarti et al., J Cardiovasc Pharmacol 2007 : Oxidized dipyridamole had no effect on TNFalpha mediated NF kappa B activation
Anand et al., Biochem Pharmacol 2010 : When examined by electrophoretic gel shift mobility assay, curcumin ( NP ) was more active than curcumin in inhibiting TNF induced NF-kappaB activation and in suppression of NF-kappaB regulated proteins involved in cell proliferation ( cyclin D1 ), invasion ( MMP-9 ), and angiogenesis ( VEGF )
Itoh et al., J Biol Chem 2005 (Inflammation) : Finally, Dok-4 enhanced TNF-alpha mediated NF-kappaB activation, whereas this was inhibited by transfection with Dok-4 small interfering RNA ... These data suggest a role for mitochondrial Dok-4 as an anchoring molecule for the tyrosine kinase c-Src, and in turn as a regulator of TNF-alpha mediated ROS production and NF-kappaB activation
Hanson et al., J Biol Chem 2003 : Oncoprotein suppression of tumor necrosis factor induced NF kappa B activation is independent of Raf controlled pathways ... This suppression of NF kappa B is manifested by an inhibition of TNF induced inhibitor of NF kappa B ( IKK ) activity and NF kappa B DNA binding potential but not by blocking TNF induced nuclear accumulation of NF kappa B/p65
Hirahashi et al., Kidney Int 2000 : TNF-alpha induced NF-kappa B activation was blocked by overexpression of I kappa B delta N. Overexpression of I kappa B delta N potentiated TNF-alpha induced apoptosis compared to mock transfection, and the potentiation was abolished by treatment with a caspase-3 inhibitor, Z-DEVD-FMK
Verstrepen et al., Biochem Pharmacol 2009 : Overexpression of ABINs inhibits NF-kappaB activation by tumor necrosis factor (TNF) and several other stimuli
Chen et al., Inflamm Res 2009 : Sulforaphane had no effect on TNF-alpha induced NF-kappaB nuclear binding activity, IkappaB-alpha degradation or activation of NF-kappaB-driven transcriptional activity
Farley et al., J Invest Dermatol 2006 (Dermatitis...) : In HaCaT cells and in reconstructed human epidermis ( RHE ), FasL triggered a NF-kappaB dependent mRNA accumulation of inflammatory cytokines ( tumor necrosis factor-alpha , IL-6, and IL-1beta ), chemokines ( CCL2/MCP-1, CXCL1/GROalpha, CXCL3/GROgamma, and CXCL8/IL-8 ), and the adhesion molecule ICAM-1
Chanson et al., Am J Pathol 2001 (Cystic Fibrosis) : TNF-alpha induced maximal translocation of NF-kappaB into the nucleus of non-CF as well as CF airway cells within 20 minutes
Devchand et al., FASEB J 2003 (Inflammation...) : hALX transfected HEK293 cells transmitted LXA4 signals that inhibit TNFalpha induced NFkappaB activation
Fessler et al., J Virol 2004 : Previous findings from this laboratory demonstrated that the E3 proteins 10.4K and 14.5K, which form a complex in the plasma membrane, inhibit tumor necrosis factor (TNF) induced activation of NF-kappaB and the synthesis of chemokines
Schreck et al., EMBO J 1991 : NAC and other thiol compounds also blocked the activation of NF-kappa B by cycloheximide, double stranded RNA, calcium ionophore, TNF-alpha , active phorbol ester, interleukin-1, lipopolysaccharide and lectin
Mo et al., J Ethnopharmacol 2007 : CML-1 inhibits TNF-alpha induced NF-kappaB activation and adhesion molecule expression in endothelial cells through inhibition of IkBalpha kinase
Oliveira et al., J Biol Chem 2008 : The increase in TNFalpha mediated cell killing observed in hSMG-1 depleted cells is not related to the suppression of nonsense mediated mRNA decay or to the inhibition of TNFalpha induced NF-kappaB activation
Yang et al., Chin J Cancer 2002 (Liver Neoplasms) : 2. TPCK could inhibit the activation of NF-kappa B induced by TNF-alpha
Williams et al., J Biol Chem 2005 (Inflammation) : Reducing Monarch-1 expression with small interference RNA in myeloid/monocytic cells caused a dramatic increase in NFkappaB activation and cytokine expression in response to TLR2/TLR4 agonists, TNFalpha , or M. tuberculosis infection, suggesting that Monarch-1 is a negative regulator of inflammation
Jana et al., Free Radic Biol Med 2005 : All three cytokines alone induced the activation of AP-1 while IL-1beta and TNF-alpha but not IFN-gamma induced the activation of NF-kappaB
Yang et al., Mol Pharmacol 2001 : We previously showed that green tea polyphenols inhibited endotoxin mediated tumor necrosis factor-alpha (TNF alpha) production by blocking NF-kappa B activation ... IEC-6 cells pretreated with an extract of green tea polyphenols ( GrTPs ; 0 -- 0.4 mg/ml ) had diminished TNF alpha induced IKK and NF-kappa B activity
Ramana et al., FASEB J 2004 (Inflammation) : Inhibition of AR abrogated TNF-alpha induced activation and membrane translocation of PKC, and antisense ablation of AR prevented both TNF-alpha induced PKC and NF-kappaB activation
Sethi et al., Mol Cancer Res 2008 : As examined by DNA binding, we found that TQ suppressed tumor necrosis factor induced NF-kappa B activation in a dose- and time dependent manner and inhibited NF-kappaB activation induced by various carcinogens and inflammatory stimuli
McDonald et al., Biochemistry 1998 : To circumvent this problem, we developed an alternative procedure which allowed us to show that in neutrophils, LPS and TNFalpha induce a NF-kappaB DNA binding activity which essentially consists of p50/RelA dimers, and that IFNgamma promotes the binding of STAT1 homodimers to the IFNgamma response region of the FcgammaRI promoter
Littlejohn et al., Biochem Pharmacol 2003 : TNF can stimulate the proinflammatory transcription factor nuclear factor-kappaB (NF-kappaB) as well as the stress induced kinase c-Jun N-terminal kinase (JNK) through mechanisms that are not fully delineated
Langen et al., FASEB J 2001 (Cachexia) : TNF-alpha activated nuclear factor-kappaB (NF-kappaB) and interfered with the expression of muscle proteins in differentiating myoblasts
Isaacs et al., J Cell Physiol 1999 : NaS decreased TNFalpha stimulated NFkappaB activation by 50 % as assessed by EMSA
Kamata et al., FEBS Lett 2002 : Electrophoretic mobility shift assay revealed that H2O2 enhanced TNF induced NF-kappaB activation
Tsytsykova et al., J Biol Chem 2007 : We find that TNF mRNA production in response to ionophore is NF-kappaB independent, but inhibition of NF-kappaB activation attenuates virus- and LPS induced TNF mRNA levels after initial induction
Diel et al., J Virol 2010 (Ecthyma, Contagious) : Expression of ORFV024 in cell cultures significantly decreased lipopolysaccharide (LPS)- and tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB-responsive reporter gene expression ... Further, ORFV024 expression decreased TNF-alpha induced phosphorylation and nuclear translocation of NF-kappaB-p65 , phosphorylation, and degradation of IkappaBalpha, and phosphorylation of IkappaB kinase (IKK) subunits IKKalpha and IKKbeta, indicating that ORFV024 functions by inhibiting activation of IKKs, the bottleneck for most NF-kappaB activating stimuli
Al-Lamki et al., Am J Pathol 2010 (Carcinoma, Renal Cell) : In ccRCC organ cultures, R1-TNF increases TNFR1, activates apoptotic signaling kinase and NF-kappaB , and promotes apoptosis in malignant TECs
Seo et al., Oncogene 2004 : vIRF3 repressed NF-kappaB dependent transcription in a dose dependent manner and inhibited the activation of NF-kappaB induced by tumor necrosis factor (TNF)-alpha
Tanaka et al., Clin Exp Immunol 2010 (Acute Lung Injury) : In the ARDS model, vitamin K3 also suppressed the LPS induced increase in the serum TNF-alpha level and inhibited the LPS evoked nuclear translocation of NF-kappaB in lung tissue
Sugii et al., Biol Pharm Bull 2005 : Treatments of xanthoangelol D but not xanthoangelol, xanthoangelol E and F markedly suppressed both of basal and tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activation in PAECs
Elmali et al., Inflammation 2007 (Arthritis, Experimental...) : Resveratrol, a polyphenolic, natural phytoalexin found with particularly high levels in grape skin and red wine is potent and specific inhibitor of TNF-alpha and IL-1beta induced NF-kappaB activation
Rui et al., J Immunol 2007 : In this study, we demonstrate that PECAM-1 ligation with CD38-Fc fusion protein negatively regulates LPS induced proinflammatory cytokine TNF-alpha , IL-6, and IFN-beta production by inhibiting JNK, NF-kappaB , and IFN regulatory factor 3 activation in macrophages
Seki et al., Hepatology 2005 : In Myd88 ( -/- ) mice after PH, induction of expression of immediate early genes involved in hepatocyte replication and phosphorylation of STAT3 in the liver, and production of TNF-alpha/IL-6 by and activation of NF-kappaB in the Kupffer cells were grossly subnormal and were associated with impaired liver regeneration
Lendemans et al., J Endotoxin Res 2006 : In contrast, the broad-spectrum tyrosine kinase inhibitor genistein and the MEK-1 inhibitor ( PD98059 ) abrogated GM-CSF priming of TNF-alpha release and activation of both NF-kappaB and AP-1
Chang et al., Mol Immunol 2007 : TSA1 could also inhibit TNF induced cytotoxicity on L929 cells and TNF mediated NF-kappaB activation on HEK-293T cells
Prasad et al., J Neurochem 2005 : It inhibits tumor necrosis factor alpha (TNFalpha) induced PI3-kinase, Akt and NF-kappaB activation in these cells
Yao et al., Dig Liver Dis 2009 (Stomach Neoplasms) : However, TNFalpha induced activation of NF-kappaB was not affected by over activated Notch-1
Kobuchi et al., Am J Physiol 1999 : Quercetin had no effect on PMA- or TNF-alpha induced nuclear factor-kappaB (NF-kappaB) activation
Kamata et al., FEBS Lett 2001 : Here we studied the effect of cPrG.HCl on TNFalpha induced activation of the transcription factor nuclear factor kappaB (NF-kappaB) ... cPrG.HCl inhibited neither the TNFalpha induced phosphorylation and degradation of inhibitor of nuclear factor-kappaB, nor the subsequent nuclear translocation and DNA binding of NF-kappaB
Wullaert et al., Hepatology 2005 (Liver Failure, Acute) : This dual function of NF-kappaB emphasizes the need for therapeutics that can inhibit both TNF induced NF-kappaB activation and cell death ... Indeed, ABIN-1 was found to prevent not only NF-kappaB activation, but also apoptosis of cultured hepatocytes in response to TNF , explaining its protective effect against TNF induced liver failure
Majumder et al., J Neurosci Res 1998 (Astrocytoma) : TNF-alpha induced NFkappaB binding activity at this site was composed of RelA ( p65 ) homodimers
Asschert et al., Int J Cancer 1999 (Ovarian Neoplasms) : Nuclear factor kappa-B (NF-KB) , which consisted mainly of p65-NF-KB was induced in response to TNF-alpha stimulation ... TNF-alpha stimulation enhanced AP-1 and induced NF-KB but no NF-IL6 DNA binding in these cells
Chaturvedi et al., J Biol Chem 1994 (Fibrosarcoma...) : By using receptor blocking antibodies we found that both p60 and p80 forms of TNF receptors were functional for NBT reducing activity, but TNF dependent NF-kappa B activation required only the p60 receptor
Zhang et al., Transplantation 2008 : TNF receptor signaling was required for activation of NF-kappaB , but not for activation of activating protein 1 family members junD and Fra-1 in day 2 allografts
Stangl et al., Biochem Biophys Res Commun 2001 : Homocysteine attenuated TNF-alpha stimulated U-937 adhesion to the endothelial monolayer and reduced TNF-alpha induced activation of the transcription factor NF-kappaB , indicating that NF-kappaB inhibition may play a role in inhibiting expression of adhesion molecules in endothelial cells
Peng et al., J Biol Chem 1995 : Electrophoretic mobility shift assays demonstrated that activation of NF-kappa B by L-NMA, ox-LDL, and TNF alpha was attenuated by GSNO and SNP, but not by glutathione or cGMP analogues
Takada et al., Oncogene 2005 (Neoplasm Invasiveness...) : We found that zerumbone suppressed NF-kappaB activation induced by tumor necrosis factor (TNF) , okadaic acid, cigarette smoke condensate, phorbol myristate acetate, and H2O2 and that the suppression was not cell type specific
Rudolph et al., Genes Dev 2000 (Fetal Death) : NEMO/IKKgamma-deficient primary murine embryonic fibroblasts ( MEFs ) lack detectable NF-kappaB DNA binding activity in response to TNFalpha , IL-1, LPS, and Poly ( IC ) and do not show stimulus dependent IkappaB kinase activity, which correlates with a lack of phosphorylation and degradation of IkappaBalpha
Ozsoy et al., J Biol Chem 2008 : The changes in TNFR self-association were functionally significant, because pretreating the HeLa cells and 293T cells resulted in increased TNF induced NF-kappaB activation and TNF induced expression of IkappaB and syndecan-4 mRNA levels ... Although pretreatment with DsbA did not result in an increase in TNF binding to TNFRs, it resulted in increased TNF induced activation of NF-kappaB , consistent with an allosteric modification of the TNFRs. Taken together, these results suggest that oxidative stress promotes TNFR receptor self-interaction and ligand independent and enhanced ligand dependent TNF signaling
Coward et al., J Immunol 2002 : The possible participation of TNF-alpha generated by mast cells in NF-kappaB activation by anti-IgE was investigated using a blocking Ab for TNF-alpha
Russo et al., Int J Radiat Oncol Biol Phys 2001 (Colorectal Neoplasms) : NF-kappaB is activated by tumor necrosis factor , certain chemotherapeutic agents, and ionizing radiation, leading to inhibition of apoptosis
Sakamoto et al., J Clin Endocrinol Metab 2003 (Endometriosis) : We hypothesize that TNFalpha may induce IL-8 production in endometriotic cells through nuclear factor-kappa B (NF-kappa B) activation ... The current study showed for the first time that GnRHa treatment attenuated the expression of IL-8 by reducing TNFalpha induced NF-kappa B activation
Zhou et al., Zhonghua Wai Ke Za Zhi 2004 (Adenocarcinoma...) : IND may inhibit the activation of NF-kappaB , decrease serum TNF-alpha and IL-6 levels and thus alleviate the cachexia
Mitsuta et al., Allergy Asthma Proc 2003 (Asthma...) : Our results indicated that TNF-alpha production was induced by D. farinae in PBMCs of patients with atopic asthma by the activation of NF-kappa B via CD23
Thirunavukkarasu et al., Hepatology 2006 (Disease Models, Animal...) : Inhibition of p38 and NF-kappaB activation by SB203580 and pyrrolidine dithiocarbamate, respectively, blocked endotoxin induced H ( 2 ) O ( 2 ), NO, TNF-alpha , and IL-6 synthesis ... In conclusion, endotoxin induced synthesis of NO, TNF-alpha , and IL-6 in HSCs is mediated by p38 and NF-kappaB , with involvement of H ( 2 ) O ( 2 ) in TNF-alpha production
Menon et al., J Biol Chem 1993 : Inability to induce NF-kappa B can not be due to a non-activatable system since NF-kappa B was strongly activated by tumor necrosis factor in all the five primary cell types tested ... Conversely cysteine, an antioxidant and precursor of the free radical scavenger, glutathione, inhibited the induction of NF-kappa B by tumor necrosis factor in primary cells, and by okadaic acid or tumor necrosis factor in transformed cells ... The induction of NF-kappa B by tumor necrosis factor in primary cells suggests that this cytokine fulfills the requirement for oxidation, possibly by inducing the production of free radicals
Deering et al., Clinical and vaccine immunology : CVI 2006 : Tumor necrosis factor (TNF) production in PBMC supernatants was measured by an enzyme linked immunosorbent assay after TLR ligand stimulation and was dependent on gene transcription and NF-kappaB activation
Rajesh et al., Am J Physiol Heart Circ Physiol 2007 (Disease Models, Animal...) : TNF-alpha induced NF-kappaB and RhoA activation and upregulation of adhesion molecules ICAM-1 and VCAM-1, increased expression of monocyte chemoattractant protein, enhanced transendothelial migration of monocytes, and augmented monocyte-endothelial adhesion
Lee et al., Infect Immun 2005 : Our data indicate that the increased Ets/Elk and NF-kappaB promoter activities associated with M. smegmatis infected macrophages are responsible , at least in part, for the increased TNF-alpha and NOS2 production observed in these infected cells and that ERK1/2 is required for Ets/Elk activity and full TNF-alpha production
Segal et al., PloS one 2010 (Granulomatous Disease, Chronic...) : When challenged with either intratracheal zymosan or LPS, NADPH oxidase-deficient p47 ( phox-/- ) mice and gp91(phox)-deficient mice developed exaggerated and progressive lung inflammation, augmented NF-kappaB activation , and elevated downstream pro-inflammatory cytokines ( TNF-alpha , IL-17, and G-CSF ) compared to wildtype mice
Sato et al., J Rheumatol 1996 (Arthritis, Rheumatoid) : Our data suggest that TNF-alpha induces expression of proinflammatory cytokines such as IL-8 and MCP-1 through generation of reactive oxygen intermediates and subsequent activation of NF-kappa B in human synovial cells, and the antioxidants may inhibit, at least in part, the activation of NF-kappa B by TNF-alpha
Jin et al., Proc Natl Acad Sci U S A 2004 : Furthermore, the activation of NF-kappaB by TAB3 was blocked by the NF-kappaB inhibitor, SN50, and by expression of dominant negative forms of tumor necrosis factor alpha associated factor 6 and transforming growth factor beta activated kinase
Carlson et al., Am J Physiol Gastrointest Liver Physiol 2007 (MAP Kinase Signaling System) : fMLP pretreatment also inhibited activation of proinflammatory transcription factor NF-kappaB by TNF-alpha in Caco2bbe cells, reducing induction of NF-kappaB target genes by TNF-alpha both in human intestinal biopsies and Caco2bbe cells
You et al., J Exp Med 2001 : In contrast, IL-1/TNF stimulated nuclear factor (NF)-kappa B DNA binding activity and inhibitor of kappa B ( I kappa B ) phosphorylation was dramatically decreased in Shp-2 ( -/ ) - cells, while the expression and activity of NF-kappa B-inducing kinase (NIK), Akt, and I kappa B kinase (IKK) were not changed
Sethi et al., Oncogene 2007 (Adenocarcinoma...) : Although DN-IKKbeta inhibited TNF induced NF-kappaB activity, DN-IKKbeta had no effect on EGF induced NF-kappaB activation, suggesting that EGF induced NF-kappaB activation is IKK independent
Sarkar et al., J Biol Chem 2004 (Breast Neoplasms...) : Our results show that mangiferin blocks tumor necrosis factor (TNF) induced NF-kappaB activation and NF-kappaB dependent genes like ICAM1 and COX2
Garbán et al., J Biol Chem 2001 (Ovarian Neoplasms) : TNF-alpha can also promote nuclear factor kappaB (NF-kappaB) activity and regulate the expression of genes that interfere with apoptosis and thus conferring resistance to several apoptotic stimuli ... These findings show that NO might interfere with TNF-alpha dependent NF-kappaB activation by interacting with O ( 2 ) and reducing the generation of H ( 2 ) O ( 2 ), a potent NF-kappaB activator
Simeonova et al., Lab Invest 1999 : Furthermore using cell transfection and mobility shift assays, it was found that transcriptional activation of the IL-8 gene required TNF-alpha induced activation and binding of nuclear factor-kappaB (NF-kappaB)- and NF-IL-6, nuclear transcription factors to regulatory elements in the IL-8 promoter
Wang et al., Clin Chim Acta 2005 : However, the effects of statin on the expression of TNF-alpha and activation of NF-kappaB in endothelial cells stimulated by CRP are less studied ... CRP stimulation result in induction of TNF-alpha and activation of NF-kappaB , and this effect could be significantly inhibited by fluvastatin, suggesting that CRP may play a direct role in atherogenesis by activating endothelial cells, and statins inhibit this response, which may provide an insight into the mechanisms of anti-inflammatory or anti-atherosclerotic actions of statins
Martinon et al., FEBS Lett 2000 : We show that the serine/threonine kinase RIP that is required for TNF induced NF-kappaB activation is processed by caspase-8 into a dominant negative ( DN ) fragment during death receptor induced apoptosis, thereby leading to a blockade of NF-kappaB mediated anti-apoptotic signals
Liuwantara et al., Diabetes 2006 : Activation of NF-kappaB by TNF receptor associated factor (TRAF) 2, TRAF6, NF-kappaB inducing kinase, or protein kinase D, which transduce signals downstream of Toll-like receptors, TNF receptors, and free radicals, respectively, were all potent activators of the A20 promoter
Andoh et al., Int J Mol Med 2002 (Acute Disease...) : Furthermore, somatostatin suppressed TNF-alpha induced NF-kappaB activation
Varfolomeev et al., Cell 2007 (Neoplasms) : IAP antagonists induce autoubiquitination of c-IAPs, NF-kappaB activation , and TNFalpha dependent apoptosis
Vince et al., Cell 2007 : In sensitive tumor lines, IAP antagonist induced NF-kappaB stimulated production of TNFalpha that killed cells in an autocrine fashion ... Inhibition of NF-kappaB reduced TNFalpha production, and blocking NF-kappaB activation or TNFalpha allowed tumor cells to survive IAC induced apoptosis
Ha et al., Exp Cell Res 2004 : In contrast, antioxidants did not prevent TNF-alpha induced Akt and NF-kappaB activation
Jackman et al., Exp Cell Res 2009 : We have investigated a well studied signaling pathway, TNF-alpha induced NF-kappaB activation, and found a connection between the stability of microtubules and the regulation of NF-kappaB signaling in C2C12 myotubes ... Although there was no additive effect of taxol and TNF-alpha on NF-kappaB activity suggesting a shared mechanism of activation, taxol strongly induced the NF-kappaB reporter in the presence of a TNF receptor (TNFR) blocking antibody while TNF-alpha did not ... Both TNF-alpha and taxol induce the degradation of endogenous IkappaBalpha and either taxol or TNF-alpha induction of NF-kappaB activity was blocked by inhibitors of NF-kappaB acting at different sites in the signaling pathway ... Both TNF-alpha and taxol strongly induce known NF-kappaB chemokine target genes ... On the other hand, if microtubules are destabilized by colchicine, then the induction of NF-kappaB by TNF-alpha or taxol is greatly reduced
Dasgupta et al., J Neurosci Res 2009 : A thorough screening of the signaling system confirmed that TNFalpha/IL-1beta stimulation up-regulated nuclear factor kappaB (NFkappaB) signaling in SV-HCECs
Garrouste et al., Cell Death Differ 2002 : Correspondingly, IGF-I markedly enhanced the TNF- and IFN/TNF induced NF-kappaB dependent interleukin-8 production
Lora et al., J Biol Chem 2005 (Cystic Fibrosis) : In vivo, TNF-alpha induced NF-kappaB as determined by whole mouse body bioluminescence
Gilston et al., Free radical research 2001 : NF-kappaB was activated by TNF-alpha in all four cell types tested
Sancho et al., Mol Pharmacol 2003 : This study shows that AEA inhibits tumor necrosis factor-alpha (TNFalpha) induced NF-kappaB activation by direct inhibition of the IkappaB kinase (IKK)beta and, to a lesser extent, the IKKalpha subunits of kappaB inhibitor ( IkappaB) kinase complex, and that IKKs inhibition by AEA correlates with inhibition of IkappaBalpha degradation, NF-kappaB binding to DNA, and NF-kappaB dependent transcription in TNFalpha stimulated cells
Paludan et al., Eur Cytokine Netw 2000 (Herpes Genitalis) : The results indicate that TNF-alpha , secreted by virus infected macrophages, activates NF-kappa B which impairs the IL-13 mediated inhibition of inducible NO synthase (iNOS) expression
Zhong et al., Zhonghua Yi Xue Za Zhi 1999 : To investigate whether TNF-alpha could activate the signaling pathway-NF-kappa B/I-kappa B alpha required for the expression of inducible nitric oxide synthase (iNOS) to induce endothelial cell apoptosis by nitric oxide ( NO )
Adya et al., Diabetes Care 2008 (Diabetes Mellitus, Type 2...) : We also found a significant inhibition of tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activity by visfatin ( P < 0.001 )
Kohno et al., J Rheumatol 2001 : Expression of interleukin 6 (IL-6) and IL-8 mRNA was examined by real-time quantitative reverse transcriptase-polymerase chain reaction analysis and TNF-alpha induced nuclear factor-kappaB (NF-kappaB) activation was observed using immunostaining with an antibody against NF-kappaB p65
Matsumura et al., Cell cycle (Georgetown, Tex.) 2003 : c-Myc and E2F1 inhibit NF-kappaB activities induced by TNFalpha or reactive oxygen species
Tapia et al., Free Radic Biol Med 2003 : It is concluded that T ( 3 ) -induced oxidative stress enhances the DNA binding activity of NF-kappaB and the NF-kappaB dependent expression of TNF-alpha and IL-10 genes
Kelly et al., AIDS 1998 (AIDS-Related Opportunistic Infections...) : Tat and TNFalpha activated comparable levels of NF-kappaB
Sun et al., Cell Signal 2009 : PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha induced IKKbeta-NF-kappaB activation ... Furthermore, knockdown of PPM1A and PPM1B expression enhances TNFalpha induced IKKbeta phosphorylation, NF-kappaB nuclear translocation and NF-kappaB dependent gene expression ... These data suggest that PPM1A and PPM1B play an important role in the termination of TNFalpha mediated NF-kappaB activation through dephosphorylating and inactivating IKKbeta
Chen et al., J Cell Biochem 2003 : Our results suggest that NF-kappaB activation in PIMs followed by phagocytizing lipopolysaccharide resulted in the up-regulation of TNF-alpha , IL-1beta, IL-6, IL-8, and COX-2, which could be alleviated by dexamethasone
Xu et al., Brain Res Mol Brain Res 1998 (Spinal Cord Injuries) : Methylprednisolone inhibition of TNF-alpha expression and NF-kB activation after spinal cord injury in rats
Holden et al., Med Hypotheses 1999 (Disease Progression...) : In addition, it has been established that tumor necrosis factor-alpha (TNF-alpha) can activate the expression of wild type p53 in concert with the nuclear transcription factor, NF-kappa B
Dbaibo et al., J Biol Chem 1993 (Leukemia-Lymphoma, Adult T-Cell) : In this cell line, TNF-alpha caused potent activation of nuclear factor-kappa B (NF-kappa B) and exerted potent cytostatic/cytocidal activity ... C2-ceramide, however, enhanced activation of NF-kappa B in response to TNF-alpha with peak effects observed at a concentration of C2-ceramide of 5 microM
Van 't Hof et al., J Bone Miner Res 2004 (Bone Resorption) : Mechanistic studies showed that ABD056 caused osteoclast apoptosis and inhibited TNFalpha induced NF-kappaB activation
Nakajima et al., Neurosci Lett 2006 : Taken together, these results suggest that the induction of IL-1beta and TNFalpha in endotoxin stimulated microglia is differentially regulated at the level of NFkappaB activation
Lee et al., Eur J Immunol 1998 : ROI have been implicated in the activation of NF-kappaB as well as c-Jun N-terminal kinase (JNK) in response to IL-1 and TNF-alpha stimulation
Moon et al., Cancer Lett 2009 (Neoplasms) : Sulforaphane suppresses TNF-alpha mediated activation of NF-kappaB and induces apoptosis through activation of reactive oxygen species dependent caspase-3 ... We also report that SFN non-specifically inhibited TNF-alpha induced NF-kappaB activation through the inhibition of IkappaBalpha phosphorylation, IkappaBalpha degradation, and p65 nuclear translocation ... These effects suggest that SFN inhibits TNF-alpha induced NF-kappaB activation through the suppression of IkappaBalpha degradation, leading to reduced expression of NF-kappaB regulated gene products ... In conclusion, the results of the present study indicate that SFN suppresses TNF-alpha induced NF-kappaB activity and induces apoptosis through activation of ROS dependent caspase-3
Meusch et al., Arthritis Rheum 2009 (Arthritis, Rheumatoid) : Instead, transmembrane TNF reverse signaling inhibited the constitutive NF-kappaB activation in RA monocytes, suppressed IL-1beta secretion, and normalized spontaneous in vitro apoptosis
van Uden et al., Biochem J 2008 : Finally, when endogenous NF-kappaB is induced by TNFalpha ( tumour necrosis factor alpha ) treatment, HIF-1alpha levels also change in an NF-kappaB dependent manner ... In conclusion, we find that NF-kappaB can regulate basal TNFalpha and, in certain circumstances, the hypoxia induced HIF-1alpha
Saile et al., Eur J Cell Biol 2001 : TGF-beta as well as TNF-alpha induced activation of NFKB and upregulated bcl-xL
Ishiguro et al., Cell Signal 2006 : RNA interference against Nore1B attenuates NF-kappaB activation induced by T cell receptor ( TCR ) ligation, but not NF-kappaB activation induced by TNFalpha or lipoteichoic acid
Kang et al., FEBS Lett 2003 : Moreover, silymarin suppressed the TNF-alpha induced DNA binding of NF-kappaB/Rel in HUVECs
Lum et al., Biochem Pharmacol 1998 : CVT-634 ( 5-methoxy-1-indanone-3-acetyl-leu-D-leu-1-indanylamide ) prevented lipopolysaccharide (LPS), tumor necrosis factor (TNF)- , and phorbol ester induced activation of nuclear factor kappa B (NF-kappa B) in vitro by preventing signal induced degradation of I kappa B-alpha
Che et al., Circ Res 2002 : Insulin-like growth factor-1 enhances inflammatory responses in endothelial cells : role of Gab1 and MEKK3 in TNF-alpha induced c-Jun and NF-kappaB activation and adhesion molecule expression ... Because Gab1 is involved in both c-Jun and NF-kappaB activation by TNF-alpha , we focused on Gab1 dependent signaling ... Gab1 associated with MEKK3, and a catalytically inactive form of MEKK3 inhibited TNF-alpha induced c-Jun and NF-kappaB transcriptional activation, suggesting a critical role for Gab1 and MEKK3 in TNF-alpha signaling
Shrivastava et al., J Virol 1998 : Stable cell transfectants expressing the HCV core protein suppressed tumor necrosis factor (TNF) induced NF-kappaB activation
Kwon et al., Mol Cell Biol 2004 : Tumor necrosis factor alpha induction of NF-kappaB requires the novel coactivator SIMPL ... These data support a model in which TNF-alpha activation of NF-kappaB dependent-gene expression requires nuclear relocalization of p65 as well as nuclear relocalization of SIMPL, generating a TNF-alpha-specific induction of gene expression
Fukuyama et al., Mol Carcinog 2007 (Colorectal Neoplasms) : RKO cells exhibit a robust, oscillatory NFkappaB activity in response to TNFalpha not seen in either of the NFkappaB mutant cell lines, which instead demonstrate diminished, nonoscillatory NFkappaB activation
Paik et al., J Biol Chem 2000 (Adenocarcinoma...) : An activator of peroxisome proliferator activated receptor gamma, 15-deoxy-Delta ( 12,14 ) -prostaglandin J ( 2 ), also induces COX-2 expression and inhibits TNFalpha induced NFkappaB activation and COX-2 expression
Loewe et al., J Invest Dermatol 2001 (Psoriasis) : Using CD62E, NF-kappa B, or AP-1-responsive promoter constructs, dimethylfumarate inhibited tumor-necrosis-factor induced activation of the CD62E and the NF-kappa B but not the AP-1 promoter construct
Delfino et al., J Biol Chem 1999 : Stimulation of Sertoli cells with tumor necrosis factor-alpha , an NF-kappaB activating cytokine produced by round spermatids located adjacent to Sertoli cells, stimulated the elimination of IkappaB, the translocation of additional NF-kappaB to the nucleus, and increased NF-kappaB binding to CREB promoter kappaB enhancer elements
Revilla et al., J Biol Chem 1998 : NF-kappaB activation induced by tumor necrosis factor , as detected by mobility shift assays or by transfection of kappaB-driven reporter genes, is impaired in African swine fever virus infected cells
Guerrini et al., FEBS Lett 1996 : NF-kappa B-mediated regulation of urokinase gene expression by PMA and TNF-alpha in human A549 cells
Shatrov et al., Biochem Biophys Res Commun 1997 (Leukemia, Myeloid) : Moreover, we have shown that while an intracellular calcium chelator BAPTA/AM was able to inhibit both SPP- and TG-induced NF-kappa B activation, it had no effect on TNF induced NF-kappa B activation
Kasinski et al., Mol Pharmacol 2008 : The effective inhibition of TNF-alpha induced NF-kappaB signaling by EF24 extends the therapeutic application of EF24 to other NF-kappaB dependent diseases, including inflammatory diseases such as rheumatoid arthritis
Hohmann et al., J Biol Chem 1990 : Both the types A and B receptors mediated TNF alpha induced activation of the transcription factor NF-kappa B
Mahboubi et al., J Pharmacol Exp Ther 1998 : Because the PGHS-2 promoter has a nuclear factor-kappa B (NF-kappa B) binding motif, which is important for PGHS-2 gene transcription in some cell types, we have evaluated the effects of tyrosine kinase inhibitors and PAO on TNF-alpha induced NF-kappa B activation ... N-tosyl-L-phenylalanine chloromethyl ketone ( TPCK ), a serine protease inhibitor, inhibited I kappa B-alpha degradation and NF-kappa B activation in response to TNF-alpha in a dose dependent manner ( 25, 50, 100 microM ) ... These data demonstrate that tyrosine kinase pathways are not required for TNF-alpha induced NF-kappa B activation in MCA-101 cells and suggest that signaling via these pathways mediates TNF-alpha induced PGHS-2 mRNA accumulation via an NF-kappa B-independent mechanism
Omori et al., Free radical research 2002 : TNF-alpha stimulation also induced marked activation of nuclear factor KB ( NF-kappaB )
Nishiumi et al., Toxicological sciences : an official journal of the Society of Toxicology 2010 (Insulin Resistance) : TCDD also stimulated the phosphorylation of extracellular signal regulated kinase ( ERK ) 1/2 and c-Jun N-terminal kinase (JNK), and their inhibitors abrogated the TCDD induced downregulation of IRbeta, IRS1, and GLUT4 ; upregulation of TNF-alpha ; and activation of NF-kappaB
Kim et al., Liver 2000 : Pretreatment of rats with DDB prevented LPS induced hepatic I-kappaBalpha degradation and the resultant NF-kappaB activation, and inhibited the LPS induced plasma TNF-alpha protein and hepatic TNF-alpha mRNA expression in a dose dependent manner
Park et al., Carcinogenesis 2007 (Neoplasms) : PMA selectively causes the degradation of IkappaB kinases ( IKKs ) including IKK-gamma and IKK-beta, and subsequent inhibition of tumor necrosis factor (TNF) induced IKK and NF-kappaB activation in human colon cancer cell line HCT-116, but not in other gastrointestinal tract cells
Funakoshi-Tago et al., Cytokine 2009 : TRAF6 negatively regulates TNFalpha induced NF-kappaB activation ... Also, TNFalpha induced DNA binding activity and transcriptional activation of nuclear factor kappaB (NF-kappaB) were also augmented in TRAF6-deficient MEFs ... Moreover, the reintroduction of exogenous TRAF6 into TRAF6-deficient MEFs clearly suppressed TNFalpha induced IKK activation, NF-kappaB activation and subsequent cytokine expression ... Thus, these data suggest that TRAF6 negatively regulates TNFalpha induced NF-kappaB activation through its ubiquitin ligase activity
Mancuso et al., J Immunol 2002 : Mitogen activated protein kinases and NF-kappa B are involved in TNF-alpha responses to group B streptococci ... Here we investigate the role of mitogen activated protein kinases ( MAPKs ) and NF-kappa B in TNF-alpha production by human monocytes stimulated with GBS or LPS, used as a positive control ... Selective inhibitors of ERK 1/2 ( PD98059 or U0126 ), p38 ( SB203580 ), or NF-kappa B ( caffeic acid phenetyl ester ( CAPE ) ) could all significantly reduce TNF-alpha production, although none of the inhibitors used alone was able to completely prevent TNF-alpha release
Kelly et al., Peptides 2006 : Peptide functionalized beads were compared with the ability of soluble peptide alone ( alpha-MSH or GKPV ) or non functionalized beads to inhibit TNF-alpha stimulated activation of NF-kappaB
Li et al., Antioxid Redox Signal 1999 : In the present study, we analyzed the signaling pathway by which TNF-alpha activates NF-kappaB in myotubes differentiated from C2C12 and rat primary myoblasts ... We found that activation of NF-kappaB by TNF-alpha was blocked by rotenone or amytal, inhibitors of complex I of the mitochondrial respiratory chain
Shrivastava et al., Antioxid Redox Signal 1999 : TNF induced NF-kappa B activation was inhibited by both superoxide and lipid peroxide quenchers but potentiated by an hydroxyl radical quencher ... Overall, these results suggest that hydroxyl radicals mediate TNF induced apoptosis but not activation of NF-kappa B , AP-1, and JNK ; superoxide radicals mediate NF-kappa B and JNK activation but potentiate apoptosis ; and lipid peroxides are required for all the signals induced by TNF
Abe et al., Am J Respir Cell Mol Biol 2000 : In accord with promoter analyses, an electrophoretic mobility shift assay showed that CAM repressed AP-1 binding in TNF-alpha treated BET-1A cells ; however, TNF-alpha induced both AP-1 and NF-kappaB binding activities in BET-1A cells
Rahman et al., Mol Pharmacol 1999 : We tested the hypothesis that activation of protein kinase C ( PKC ) and generation of oxidants are critical sequential signals mediating tumor necrosis factor (TNF)-alpha induced activation of nuclear factor-kappaB (NF-kappaB) and transcription of the intercellular adhesion molecule (ICAM)-1 gene ... However, both PKC activation and oxidant generation were necessary for ICAM-1 mRNA expression because the pretreatment of HPAE cells with either calphostin C or N-acetylcysteine inhibited the TNF-alpha induced activation of NF-kappaB and prevented the activation of ICAM-1 promoter
Jeon et al., Proc Natl Acad Sci U S A 2010 (Pneumonia) : In particular, vinpocetine inhibits TNF-alpha induced NF-kappaB activation and the subsequent induction of proinflammatory mediators in multiple cell types, including vascular smooth muscle cells, endothelial cells, macrophages, and epithelial cells
Glauner et al., Eur J Biochem 2002 : In cells deficient for RIP ( receptor interactive protein ), an essential signaling intermediate of TNF dependent NF-kappaB activation, TNF-, but not PMA induced up-regulation of TRAF4 was blocked
Sandur et al., J Biol Chem 2006 (Inflammation) : We found that plumbagin inhibited NF-kappaB activation induced by TNF , and other carcinogens and inflammatory stimuli ( e.g. phorbol 12-myristate 13-acetate, H2O2, cigarette smoke condensate, interleukin-1beta, lipopolysaccharide, and okadaic acid )
Dimitrov et al., J Virol 1997 : Under the same conditions, Ad infection did not inhibit TNF induced phosphorylation of cPLA2 or TNF activation of NFkappaB
Lockyer et al., Circ Res 1998 : Furthermore, TNF-alpha induced NF-kappaB DNA binding activity was inhibited in the population of endothelial cells expressing IkappaBdeltaN
Wu et al., Leuk Res 2003 (Blast Crisis...) : Since nuclear factor-kappaB (NF-kappaB) is activated by TNFalpha and induces expression of prosurvival genes, effects of the antisense oligodeoxynucleotides to NF-kappaB subunits, p65 and p50, were examined
Saha et al., J Neuroimmune Pharmacol 2006 : Activation of NF-kappaB by TNF-alpha and inhibition of TNF-alpha induced BDNF expression by Deltap65 ( a dominant negative mutant ) and NEMO binding domain peptide ( an inhibitor of NF-kappaB ) suggests that TNF-alpha induces BDNF expression through the activation of NF-kappaB
Rahman et al., Blood 2003 (MAP Kinase Signaling System) : The treatment of RAW264 cells with TSA and NaB inhibited TNF-alpha induced nuclear translocation of NF-kappa B and sRANKL induced activation of p38 mitogen activated protein kinase ( MAPK ) signals
Cardile et al., Clin Exp Pharmacol Physiol 2010 (Inflammation) : 1. The novel nuclear factor (NF)-kappaB inhibitor dehydroxymethylepoxyquinomicin ( DHMEQ ) is a derivative of the antibiotic epoxyquinomicin C from Amycolatopsis sp. that has been found to inhibit tumour necrosis factor (TNF)-alpha induced activation of NF-kappaB by suppressing nuclear translocation of NF-kappaB
Raes et al., C R Seances Soc Biol Fil 1995 (Second Messenger Systems) : However several questions regarding the molecular mechanisms involved in particular steps of these cascades remain largely unresolved : how and at which subcellular level, do the cells produce these reactive oxygen intermediates that will contribute to NF kappa B activation in response to IL-1 or TNF-alpha ?
Koul et al., Biochem Biophys Res Commun 2006 (Glioma) : We found that PTEN inhibited NFkappaB activation induced by TNF
Azoitei et al., Oncogene 2007 : Here, we report that expression and consequent activation of TNK1 enables tumor necrosis factor alpha (TNFalpha) induced apoptosis by selectively inhibiting TNFalpha induced activation of nuclear factor-kappaB (NF-kappaB) ... TNK1 has no effect on NF-kappaB DNA binding or the composition of the NF-kappaB complex ; however, the kinase markedly prevents TNFalpha induced NF-kappaB transactivation
Sharma et al., Int J Cancer 2008 (Glioblastoma) : Ebselen ( 2-phenyl-1,2-benzisoselenazol-3 [ 2H ] one ) -a selenoorganic compound is known to prevent TNFalpha mediated NF-kappaB activity
Cheshire et al., Mol Cell Biol 1997 : Typically, the synergistic effect on gene expression is due to the independent activation of nuclear factor kappaB (NF-kappaB) by TNF-alpha and of signal transducers and activators of transcription or IFN-regulatory factor 1 by IFNs, allowing these transcription factors to bind their unique promoter sites ... However, since activation of NF-kappaB by TNF-alpha is often transient and would not activate long-term kappaB dependent transcription effectively, we explored the effects of IFN-gamma on TNF-alpha induced NF-kappaB activity ... IFN-gamma, which typically does not activate NF-kappaB, synergistically enhanced TNF-alpha induced NF-kappaB nuclear translocation via a mechanism that involves the induced degradation of I kappaBbeta and that apparently requires tyrosine kinase activity in preneuronal cells but not in endothelial cells
Sun et al., Science 1993 : Phorbol ester and tumor necrosis factor-alpha induction of nuclear NF-kappa B is associated with both the degradation of performed I kappa B alpha and the activation of I kappa B alpha gene expression
Kruppa et al., J Immunol 1992 : Inhibition of tumor necrosis factor (TNF) mediated NF-kappa B activation by selective blockade of the human 55-kDa TNF receptor ... mAb H398 is shown to efficiently block not only TNF- but also lymphotoxin mediated activation of NF-kappa B ... Together, the results of this study indicate that TR55 is both necessary and sufficient for mediating TNF activation of NF-kappa B
Shisler et al., J Virol 2004 : Moreover, MVA/K1L gained the ability to repress artificially contrived and natural NF-kappaB regulated expression of a transfected luciferase and the cellular tumor necrosis factor gene, respectively
Kouba et al., J Biol Chem 2001 : Tumor necrosis factor-alpha induces distinctive NF-kappa B signaling within human dermal fibroblasts ... Further, NF-kappa B activation by TNF-alpha was unaffected by overexpression of a dominant negative mutant NIK in fibroblasts, despite detection of endogenous TRAF2 and NIK by Western analysis
Ju et al., Biochem Biophys Res Commun 2009 : PGG significantly inhibited TNF-alpha/IFN-gamma induced NF-kappaB activation as well as STAT1 activation
Biswas et al., Mol Med 1994 : The mechanism of inhibitory action of PTX on virus replication and NF-kappa B-induced transactivation of HIV-1 gene expression has been elucidated as due to blocking PKC dependent PMA- or TNF-alpha induced activation of NF-kappa B in Jurkat and 293-27-2 cells
Yoo et al., Am J Physiol Lung Cell Mol Physiol 2001 (Pneumonia) : ASA inhibited TNF-alpha induced activation of NF-kappa B by preventing phosphorylation and subsequent degradation of I kappa B-alpha in a prostanoid independent manner
Manna et al., J Cell Physiol 2006 : In this report we provide evidences that oleandrin, a cardiac glycoside potentially inhibited IL-8-, formyl peptide ( FMLP ) -, EGF-, or nerve growth factor (NGF)-, but not IL-1- or TNF induced NF-kappaB activation in macrophages
Mukhopadhyay et al., J Biol Chem 2002 : Ectopic expression of protein-tyrosine kinase Bcr-Abl suppresses tumor necrosis factor (TNF) induced NF-kappa B activation and IkappaBalpha phosphorylation ... We used a bcr-abl-deficient human megakaryocytic leukemia cell line MO7E and an isogenic MBA cell line stably transfected with bcr-abl. Electrophoretic mobility shift assay revealed that TNF activated the nuclear transcription factor NF-kappaB in MO7E cells but not in MBA cells ... The suppression of TNF induced NF-kappaB activation by Bcr-Abl was not restricted to MBA cells, because ectopic expression of Bcr-Abl in human acute myeloid leukemia HL-60 cells also blocked TNF induced NF-kappaB activation
Andoh et al., Int J Mol Med 2000 : EMSAs indicated that trichostatin A weakly suppressed TNF-alpha induced NF-kappaB and NF-IL6 activation
Bowie et al., J Immunol 2000 : Vitamin C inhibits NF-kappa B activation by TNF via the activation of p38 mitogen activated protein kinase
Lu et al., J Hepatol 2008 (Disease Models, Animal...) : PDTC protects mice against BCG/LPS induced inflammatory liver injury through the repression of NF-kappaB mediated TNF-alpha release, while it seems to be detrimental in GalN/LPS induced apoptotic liver damage
Zambre et al., Bioorg Med Chem 2006 (Leukemia) : Novel curcumin analogs targeting TNF induced NF-kappaB activation and proliferation in human leukemic KBM-5 cells ... Copper ( II ) conjugates of all synthesized ligands were prepared and structurally characterized as well as evaluated for their potential of inhibiting TNF induced NF-kappaB activation and proliferation in human leukemic KBM-5 cells wherein compound 13 was found to be more potent than curcumin
Kane et al., Mol Cell Biol 2002 : This mutated form of Cot also acts as a dominant negative for T-cell antigen receptor/CD28- or Akt/phorbol myristate acetate induced NF-kappa B induction, while having relatively little effect on tumor necrosis factor induction of NF-kappa B
Kumar et al., Oncogene 1998 : Emodin ( 3-methyl-1,6,8-trihydroxyanthraquinone ) inhibits TNF induced NF-kappaB activation, IkappaB degradation, and expression of cell surface adhesion proteins in human vascular endothelial cells
Rodríguez et al., Immunogenetics 1994 (Breast Neoplasms...) : In addition, induction of NF-kB binding activity by the tumor necrosis factor was accompanied by a decrease in nuclear factors that bind to the CRE II region
Blackburn et al., Biochem J 2010 : We demonstrate that such compounds show low nanomolar IC50 values for the human 20S beta5 site in vitro, and that pharmacological inhibition of this site in cells is sufficient to potently inhibit the degradation of a tetra-ubiquitin-luciferase reporter, activation of NFkappaB ( nuclear factor kappaB ) in response to TNF-alpha ( tumour necrosis factor-alpha ) and the proliferation of cancer cells
Wang et al., Cancer 2002 (Carcinoma...) : The results showed that TNF-alpha can induce activation of NF-kappaB and that the activation and translocation of NF-kappaB into the nucleus is responsible for promoting the 3-D cytomorphologic differentiation of anaplastic thyroid carcinoma cells, which was inhibited by the NF-kappaB translocation inhibitor, NF-kappaB SN50 ... The current data suggest that TNF-alpha can induce thyrocyte differentiation in anaplastic thyroid carcinoma cells through NF-kappaB and that it merits investigation as differentiation therapy for the treatment of patients with anaplastic thyroid carcinoma
Zamorano et al., J Immunol 2003 : We previously found that IL-4 and TNF-alpha cooperate in the activation of STAT6 and NF-kappaB , suggesting that these transcription factors are regulated by common intracellular signaling pathways
Haugen et al., Endocrinology 2007 : NF-kappaB activation by globular adiponectin was not affected by antibody inhibition of toll-like receptor 4 or TNF receptors 1 and 2 but was attenuated by inhibitors of p38 MAPK, phosphatidylinositol 3-kinase, and protein kinase C. Analyses of the p65 subunit of NF-kappaB in different leukocyte cell lines showed activation of two monocytic cell lines ( U937 and THP-1 ) by native and globular adiponectin
Bowie et al., J Biol Chem 1997 : Lipid peroxidation is involved in the activation of NF-kappaB by tumor necrosis factor but not interleukin-1 in the human endothelial cell line ECV304 ... Here we have investigated the effect of a range of putative antioxidants on NF-kappaB activation by interleukin-1 and tumor necrosis factor as well as the ability of H2O2 to activate NF-kappaB in primary human umbilical vein endothelial cells and the transformed human endothelial cell line ECV304 ... Finally, butylated hydroxyanisole, which inhibits lipid peroxidation but has no iron chelating properties, inhibited NF-kappaB activation by tumor necrosis factor but not interleukin-1
Kraft et al., Int Arch Allergy Immunol 2001 (Hypersensitivity, Immediate) : Inhibitors of NF-kappa B activation such as N-acetylcysteine or N-tosyl-L-phenylalanine chloromethyl ketone can suppress Fc epsilon RI-induced TNF-alpha and MCP-1 release
Nemoto et al., Mol Cell Biol 1998 : MEKK1 is activated by tumor necrosis factor alpha (TNF-alpha) and interleukin-1 and can potentiate the stimulatory effect of TNF-alpha on IKK and NF-kappaB activation
Park et al., Mol Cells 2009 : Hepatitis delta virus large antigen sensitizes to TNF-alpha induced NF-kappaB signaling ... In this study, we demonstrated that TNF-alpha induced NF-kappaB transcriptional activation was increased by LHDAg but not by SHDAg in both HEK293 and Huh7 cells
Yang et al., J Neuroimmunol 2008 : The activation of NF-kappaB was followed by increased mRNA expression of TNF-alpha and IL-1beta peaking at about 20 h
Yu et al., Ann Clin Lab Sci 2002 : Ketamine significantly reduced the LPS induced NFkappaB activation and inhibited TNFalpha production in a dose dependent manner
Wu et al., American journal of physiology. Renal physiology 2009 (Nephritis) : Specific inhibitors of caspases-8 and -3, but not of caspase-1, reduced TNF induced NF-kappaB activation ... Consistent with this, MLC kinase (MLCK) inhibitor ML-7 reduced TNF induced NF-kappaB activation
Zhang et al., Mol Biol Cell 2010 : PLK1 inhibits NF-kappaB transcriptional activation induced by TNF-alpha , IL-1beta, or several activators, but not by nuclear transcription factor p65 ... PLK1 expression reduces the DNA binding activity of NF-kappaB induced by TNF-alpha
Zhang et al., J Immunol 2006 (Arthritis, Rheumatoid...) : The RASF derived exosomes, but not exosomes derived from fibroblasts obtained from individuals with osteoarthritis, are cytotoxic for the L929 cell, a TNF-alpha-sensitive cell line, and stimulate activation of NF-kappaB and induction of collagenase-1 in RASF
Zhang et al., Neuroscience 2009 (Alzheimer Disease) : Our findings suggest that NF-kappaB can mediate induction of COX-2, TNFalpha and astrogliosis in APPswe/PS1dE9 mice
Lee et al., Science 2000 (Cachexia...) : Failure to regulate TNF induced NF-kappaB and cell death responses in A20-deficient mice ... A20-deficient cells fail to terminate TNF induced NF-kappaB responses ... Thus, A20 is critical for limiting inflammation by terminating TNF induced NF-kappaB responses in vivo
Sheeba et al., J Ethnopharmacol 2009 : Furthermore, Cardiospermum halicacabum L. ethanol extract inhibited the TNF-alpha induced DNA binding activity of NF-kappaB , which was associated with decreased p65 protein level in the nucleus in Jurkat cells
Zhao et al., Immunol Invest 2008 (Inflammation) : The cellular secretion of TNF-alpha, IL-1beta, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-alpha , COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, and activation of NF-kappaB were assayed by ELISA, the Griess method, real-time quantitative PCR, and Western blot and immunocytochemistry method, respectively
von Bülow et al., J Immunol 2007 (MAP Kinase Signaling System) : Zinc dependent suppression of TNF-alpha production is mediated by protein kinase A-induced inhibition of Raf-1, I kappa B kinase beta, and NF-kappa B
Bonetti et al., J Neuropathol Exp Neurol 2000 (Neurilemmoma...) : By immunocytochemical and functional assays, we found that SC expressed TNF receptors and that TNFalpha promoted in SC cultures transient activation of transcription factors NFkappaB and c-jun in the absence of apoptosis
Rigby et al., Oncogene 2007 (Colonic Neoplasms...) : In vitro, SOCS3 overexpression reduced proliferation, IL-6 mediated STAT3 activation and tumor necrosis factor (TNF) alpha mediated NF-kappaB activation
Nikolaidis et al., Shock 2010 : Ron receptor tyrosine kinase negatively regulates TNFalpha production in alveolar macrophages by inhibiting NF-kappaB activity and Adam17 production
Ji et al., Zhong Xi Yi Jie He Xue Bao 2006 : QGHXR can protect liver cells by down regulating the expressions of CD14, TLR(4) and NF-kappaB and inhibiting TNF-alpha expression
Davé et al., J Immunol 2007 (Chronic Disease...) : In a NF-kappaB reporter system, 8K-NBD dose-dependently inhibits TNF induced NF-kappaB activation
Ulfhammer et al., Journal of thrombosis and haemostasis : JTH 2006 (Cardiovascular Diseases...) : Further, we provide evidence for a TNF-alpha induced binding of NF-kappaB to the recently described kappaB site in the t-PA gene and of cyclic adenosine monophosphate response element binding protein ( CREB ) to the t-PA CRE-like site
Wätjen et al., Mol Nutr Food Res 2009 (Carcinoma, Hepatocellular...) : Furthermore, enniatins A1 and B1, but not enniatin B were able to inhibit moderately tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB activation
Lerner-Marmarosh et al., FASEB J 2007 : Presently, we show that hBVR increases PKC-zeta autophosphorylation, stimulation by TNF-alpha , as well as cytokine stimulation of NF-kappaB DNA binding and promoter activity
Funakoshi-Tago et al., Mol Pharmacol 2009 : Here, we found that licochalcone A strongly inhibited tumor necrosis ( TNF)-alpha induced nuclear localization, DNA binding activity, and the transcriptional activity of nuclear factor-kappaB (NF-kappaB) ... In contrast, a structurally related compound, echinatin, failed to inhibit TNF-alpha induced IKK activation and NF-kappaB activation, suggesting that the 1,1-dimethy-2-propenyl group in licochalcone A is important for the inhibition of NF-kappaB
Shin et al., Biochem Biophys Res Commun 2009 (Disease Models, Animal...) : In addition, chrysin inhibited tumor necrosis factor (TNF)-alpha induced activation of NF-kappaB in IEC-6 cells
Wahyudi et al., Acta Med Indones 2007 (Arteriosclerosis) : We postulate that green tea polyphenols regulate TNF-alpha gene expression by modulating NF-KB activation through their inhibition effect on IKB Kinase (IKK) activity and as scavenger of free radicals
Liang et al., Mol Pharmacol 2003 : Namely, JD inhibits tumor necrosis factor-alpha induced activation of NF-kappaB in mouse 3T3 and human HeLa cells
Vogel et al., Biochim Biophys Acta 2006 : In transient transfection reporter gene assays, SSAT2 functions as a transcriptional coactivator for NF-kappaB and cooperates with CBP and P/CAF to enhance TNFalpha induced NF-kappaB activity
Cantin et al., Am J Respir Crit Care Med 2000 : The degree to which albumin increased GSH levels was sufficient to protect cells against H ( 2 ) O ( 2 ) -mediated cytotoxicity and to decrease TNF-alpha mediated NF-kappaB activation
Prasad et al., Free Radic Biol Med 2004 : Plasma zinc, MDA, HAE, and 8-OHdG levels ; LPS induced TNF-alpha and IL-1beta mRNA ; and ex vivo TNF-alpha induced NF-kappaB activity in mononuclear cells ( MNC ) were determined before and after supplementation ... Ex vivo, zinc protected MNC from TNF-alpha induced NF-kappaB activation
Yuksel et al., Thromb Haemost 2002 (MAP Kinase Signaling System...) : These observations strongly suggest that APC inhibited LPS induced TNF-alpha production by inhibiting the activation of both NF-kappa B and AP-1 and that the inhibitory activity of APC might depend on its serine protease activity
De Valck et al., Biochem Biophys Res Commun 1997 : However, A20 mutants that no longer associated with 14-3-3 proteins could still fully inhibit NF-kappaB activation induced by tumor necrosis factor , interleukin-1beta or phorbol 12-myristate 13-acetate, thus excluding a crucial role for 14-3-3 interaction in this A20 function
Kupatt et al., Circ Res 1999 (Myocardial Ischemia...) : These data suggest that ischemia and reperfusion in isolated hearts cause liberation of TNF-alpha , activation of NF-kappaB , and upregulation of ICAM-1, an adhesion molecule involved in inflammatory response after ischemia and reperfusion
Liu et al., Zhong Nan Da Xue Xue Bao Yi Xue Ban 2009 (Acute Lung Injury...) : Hemin further upregulated the expression of HO-1 mRNA, decreased NF-kappaB activity drastically, and inhibited the serum levels of TNF-alpha and IL-6 significantly ( P < 0.05 )
Facchini et al., J Cell Physiol 2005 : To search for new approaches to limit cartilage damage, we investigated the requirement of polyamines for NF-kappaB activation by TNFalpha in human C-28/I2 chondrocytes, using alpha-difluoromethylornithine ( DFMO ), a specific polyamine biosynthesis inhibitor
Swinney et al., J Biol Chem 2002 : We propose that Ro106-9920 selectively inhibits an uncharacterized but essential ubiquitination activity associated with LPS- and TNFalpha induced IkappaB(alpha) degradation and NF-kappaB activation
D'Addario et al., J Mol Biol 2000 : Furthermore, we demonstrate that activation of TNF-alpha by gp350 is mediated by NF-kappaB through signal transduction pathways involving PKC, PI3-K and tyrosine kinases
Broderick et al., Clin Immunol 2006 (Carcinoma, Non-Small-Cell Lung...) : TNF-alpha induced maximal NF-kappaB translocation in these T cells, indicating that they remain receptive to alternative signaling pathways, and pulsing with IL-12 prior to TCR triggering reversed their apparent anergy
Xie et al., Zhongguo Zhong Xi Yi Jie He Za Zhi 2006 (Asthma) : Herba Epimedii and Radix Astragali could effectively reduce the production of TNF-alpha and inhibit NF-kappa B activity, and the efficacies in the three treated group were similar, showing insignificant difference among them ... Application of Herba Epimedii and Radix Astragali in the attack or remission stage of asthma could restrain the development of inflammation by reducing the production of TNF-alpha and inhibiting NF-kappa B activity
Kim et al., Proc Natl Acad Sci U S A 2005 (Dysentery, Bacillary) : Transfection experiments indicated that OspG can prevent phospho-IkappaBalpha degradation and NF-kappaB activation induced by TNF-alpha stimulation
Choi et al., J Microbiol Biotechnol 2007 : In addition, streptochlorin inhibited TNF-alpha induced NF-kappaB activation in the newly developed cell based reporter gene assay
Bai et al., Scand J Immunol 2007 (Colitis...) : The mice with colitis treated by AN showed less tissue damage, less MPO activity, less TNF-alpha production in colon, and inhibited NF-kappaB activation in LPMC, compared with those mice with colitis untreated, whereas the mice with colitis treated by CHD showed the worst tissue damage, the highest MPO activity, the highest TNF-alpha level, and enlarged NF-kappaB activation in LPMC
Kan et al., Am J Physiol 1999 : TNF-alpha enhances cardiac myocyte NO production through MAP kinase mediated NF-kappaB activation
Smith et al., J Neuropathol Exp Neurol 2009 (Disease Models, Animal...) : These data indicate that tumor necrosis factor mediates rapid activation of injury induced NF-kappaB DNA binding in Schwann cells and that these events are associated with inhibition of postinjury axonal sprouting
Sumitomo et al., J Urol 1999 (Prostatic Neoplasms) : EMSA showed that NF-kappaB inhibitors continuously inhibited TNF-alpha induced NF-kappaB activation
Baouz et al., Int Immunol 2005 (Asthma) : Herein, we analysed the pharmacological response of lung myofibroblasts to beta2-agonists associated or not to inhaled corticosteroids, investigating their effects on ( i ) the constitutive and transforming growth factor-beta ( TGF-beta ) -induced expression of alpha-smooth muscle actin ( alpha-SMA ), the main functional marker of myofibroblastic differentiation and contractility ; ( ii ) isometric contraction and ( iii ) tumour necrosis factor-alpha (TNF-alpha) induced nuclear translocation of the pro-inflammatory transcription factor nuclear factor-kappaB (NF-kappaB) ... Second, the two drugs block the TNF-alpha induced nuclear translocation of the pro-inflammatory transcription factor NF-kappaB
Heyninck et al., Trends Biochem Sci 2005 (Inflammation) : Recently, the underlying mechanism by which A20 downregulates NF-kappaB activation in response to the pro-inflammatory cytokine tumor necrosis factor (TNF) has been described
Takami et al., Arterioscler Thromb Vasc Biol 2007 (Carotid Artery Injuries...) : Overexpression of the UCHL1 gene significantly attenuated tumor necrosis factor (TNF)-alpha induced NF-kappaB activity in vascular cells and increased inhibitor of kappa B-alpha ( IkappaB-alpha ), possibly through the attenuation of IkappaB-alpha ubiquitination, leading to decreased neointima in the balloon injured artery
Tsuneyoshi et al., Cell Immunol 2006 : The mutant penta acylated LPS from the lpxM-strain did not induce TNF-alpha production in murine peritoneal macrophages, or activation of NF-kappaB in transfected cells expressing murine TLR4/MD-2
Pan et al., Zhong Xi Yi Jie He Xue Bao 2010 (Colitis) : Up-regulating the expression of occludin and down regulating the expressions of TLR4 and NF-kappaB , and hence inhibiting TNF-alpha expression and improving the mucosa barrier function may be part of the mechanisms of BWXLS in treating oxazolone induced colitis in mice
Berman et al., Clin Cancer Res 2002 (Carcinoma, Non-Small-Cell Lung...) : Recently, sulindac and other nonsteroidal anti-inflammatory drugs have been shown to inhibit tumor necrosis factor (TNF)-alpha mediated NF-kappaB activation ... We further show that sulindac inhibits TNF-alpha mediated activation of NF-kappaB DNA binding and nuclear translocation of NF-kappaB
Moon et al., Cancer Lett 2010 (Leukemia) : Nuclear factor-kappa B (NF-kappaB) activation by tumor necrosis factor-alpha (TNF-alpha) attenuates the TNF-alpha induced apoptosis pathway ... Such sensitization is closely associated with the inhibitory effect of HA14-1 on TNF-alpha mediated NF-kappaB activation
Gironella et al., Gut 2005 (Colitis...) : PAP prevented TNF-alpha induced NFkappaB activation in monocytic, epithelial, and endothelial cells and reduced proinflammatory cytokine mRNA levels and adhesion molecule expression
Sudhakar et al., PloS one 2009 : NF-kappaB mediates tumor necrosis factor alpha induced expression of optineurin, a negative regulator of NF-kappaB ... Overexpressed optineurin partly inhibited TNFalpha induced NF-kappaB activation in Hela cells ... In addition these results suggest that there is a negative feedback loop in which TNFalpha induced NF-kappaB activity mediates expression of optineurin, which itself functions as a negative regulator of NF-kappaB
Kalkhoven et al., J Biol Chem 1996 (Neoplasms) : Furthermore, activation of NF-kappaB by tumor necrosis factor-alpha also results in repression of PR, while PR is able to repress tumor necrosis factor-alpha induced NF-kappaB activity
Abu-Amer et al., J Biol Chem 1998 : In contrast, we find TNF induction of NF-kappaB in murine bone marrow macrophages ( BMMs ), is mediated, by c-Src, in a cell, and cytokine specific manner
Shishodia et al., J Biol Chem 2004 (Inflammation...) : Guggulsterone suppressed DNA binding of NF-kappaB induced by tumor necrosis factor (TNF) , phorbol ester, okadaic acid, cigarette smoke condensate, hydrogen peroxide, and interleukin-1
Malewicz et al., J Biol Chem 2003 : In addition, NF kappa B downstream of TNFR was required for up-regulation of Fas expression by endogenous TNF secreted in response to TCR stimulation
Yasui et al., Ann N Y Acad Sci 2003 (Uterine Cervical Neoplasms) : We here demonstrate that sulindac inhibited TNF-alpha mediated NF-kappaB activation and greatly enhanced TNF-alpha induced apoptosis in human gastric MKN45 and cervical HeLa carcinoma cell lines
Hattori et al., Diabetes 2007 : gAd activated NF-kappaB and enhanced tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activity
Ruiz et al., J Nutr 2006 (Inflammation) : We demonstrated that 3'-hydroxy-flavone but not the chemical core structure flavone blocked TNF-alpha induced nuclear factor (NF)-kappaB transcriptional activity and IP-10 expression at the level of NF-kappaB/IkappaBalpha phosphorylation/degradation by inhibiting IkappaB kinase activity ... In addition to the compound-specific inhibition of TNF induced NF-kappaB DNA binding and NF-kappaB transcriptional activity, apigenin and luteolin selectively blocked Akt phosphorylation/activity
Yaron et al., EMBO J 1997 : Microinjection of the inhibitory peptides into stimulated cells abolished NF-kappa B activation in response to TNF alpha and the consequent expression of E-selectin, an NF-kappa B-dependent cell-adhesion molecule
Mihindukulasuriya et al., J Biol Chem 2004 : Protein phosphatase 4 ( PP4 ; also named PPX or PPP4 ) is a PP2A related protein serine/threonine phosphatase with important roles in a variety of cellular processes such as microtubule growth/organization, apoptosis, tumor necrosis factor (TNF)-alpha signaling, and activation of c-Jun N-terminal kinase and NF-kappaB
Schmelzer et al., Biofactors 2008 (Inflammation) : To test this hypothesis, we studied the effect of CoQ10 on the NFkappaB1 dependent pro-inflammatory cytokine TNF-alpha
Thomas et al., Cancer Res 2009 : Tumor necrosis factor (TNF) receptor associated factor 2 ( TRAF2 ) is an adaptor protein that modulates the activation of the c-Jun NH ( 2 ) terminal kinase ( JNK ) /c-Jun and IkappaB kinase (IKK)/nuclear factor-kappaB (NF-kappaB) signaling cascades in response to TNFalpha stimulation
Obara et al., Arterioscler Thromb Vasc Biol 2000 (Arteriosclerosis) : Electromobility gel shift and luciferase assays demonstrated that overexpression of IkappaBDeltaN inhibited NF-kappaB activation induced by TNF-alpha or interleukin-1beta (IL-1beta)
Sun et al., J Biol Chem 2002 : This interaction is a prerequisite for RIP3 mediated phosphorylation of RIP and subsequent attenuation of TNF induced NF-kappaB activation
Inan et al., Am J Physiol Gastrointest Liver Physiol 2000 : TNF-alpha is NF-kappaB regulated , and its upregulation in NF-kappaB1 knockouts may result from an alleviation of p50-p50 repression
Di et al., Cell Signal 2008 : Expression of non phosphorylated TAK1 interferes with MEKK3 phosphorylation and NF-kappaB reporter activity induced by transient MEKK3 expression or TNFalpha stimulation
Bond et al., FEBS Lett 1998 (Inflammation) : NF-kappaB binding was rapidly induced by IL-1alpha or TNF-alpha but was neither induced nor potentiated by bFGF or PDGF
Dwyer et al., Hypertens Res 2008 (Disease Models, Animal...) : Our data suggest a role for the Ras/mitogen activated protein kinase ( MAPK ) signaling pathway and the tumor necrosis factor (TNF) receptor mediated activation of nuclear factor-kappaB (NF-kappaB) in the etiology of cardiac enlargement in the HHR
Goetz et al., Dev Comp Immunol 2004 : TNF transcripts are expressed in both mammals and fish with similar kinetics, and the involvement of NF-kappaB in TNF expression in fish suggests that transcriptional regulation may be similar within vertebrates
O'Gorman et al., Am J Physiol Gastrointest Liver Physiol 2010 : Tumor necrosis factor-alpha induced NF-kappaB activation was blocked and IkappaBalpha expression was higher in HCT116 cells lacking DNMT than in parental cells
Eto et al., Circulation 2005 : The TNF-alpha induced increase in nuclear factor (NF)-kappaB DNA binding activity was significantly suppressed by TDZD-8 ... GSK-3 regulates TNF-alpha induced IkappaB-alpha degradation and NF-kappaB activation independent of IkappaB-kinase-beta and subsequent induction of TF and VCAM-1 expression in human endothelial cells
Factor et al., Hepatology 2001 (Liver Neoplasms, Experimental) : Lastly, activation of the Akt/protein kinase B (PKB), which has recently been implicated in NF-kappaB activation by PDGF, TNF-alpha , and Ras, was also observed
Cheong et al., J Biol Chem 2006 : We undertook an iterative computational and experimental investigation of the dynamic properties of tumor necrosis factor (TNF)alpha mediated activation of the transcription factor NF-kappaB
Park et al., J Biol Chem 2002 : Here we show that overexpression of NS5A inhibits tumor necrosis factor (TNF)-alpha induced nuclear factor kappaB (NF-kappaB) activation in HEK293 cells, as determined by luciferase reporter gene expression and by electrophoretic mobility shift assay ... NF-kappaB activation by TNF receptor associated death domain and TRAF2 was inhibited by NS5A, whereas MEKK1 and IKKbeta dependent NF-kappaB activation was not affected, suggesting that NS5A may inhibit NF-kappaB activation signaled by TRAF2 ... Our findings suggest a possible molecular mechanism that could explain the ability of NS5A to negatively regulate TNF-alpha induced NF-kappaB activation
Brennan et al., Biochim Biophys Acta 1995 : The results from this study therefore suggest that the reactive oxygen model of NF kappa B activation may be restricted to certain cell types and that the presence of such a system is not required for the activation of NF kappa B by IL1 and TNF
Manna et al., J Immunol 2000 : We tested this hypothesis by pretreating human Jurkat T cells with IFN-alpha, which blocked TNF induced activation of NF-kappaB and AP-1 in a time- and dose dependent manner as determined by EMSA
Lagunoff et al., Int Rev Immunol 2003 : Virally encoded genes inhibit the activation of caspase-8 by the TNF receptor and Fas ; activate NF-kappaB to increase expression of antiapoptotic genes ; inhibit interferon response ; bind to p53, thereby blocking p53 dependent apoptosis ; and interact with other pro- and antiapoptotic cellular genes
Cong et al., World J Gastroenterol 2002 (Inflammation) : sCCK-8 inhibits LPS induced TNF-alpha mRNA expression by regulating NF-kappaB activity in rat PIMs, which is mediated through CCK receptors and inhibiting IkappaB-alpha degradation
Handa et al., J Pharmacol Exp Ther 2004 : Stimulation of RGM-1 cells with TNF-alpha resulted in an increase in intracellular oxidative stress, NF-kappaB translocation to the nuclei, and up-regulation of CINC-1 mRNA and protein, which was prevented by interfering with mitochondria dependent ROS production and NF-kappaB activation
Amigó et al., Br J Pharmacol 2007 (Disease Models, Animal...) : Antipsoriatic effects of avarol-3'-thiosalicylate are mediated by inhibition of TNF-alpha generation and NF-kappaB activation in mouse skin
Ching et al., Biochem Biophys Res Commun 2001 : BRE, a putative stress modulating gene, found able to down-regulate TNF-alpha induced NF-kappaB activation upon overexpression, is now shown in human cells expressed as multiple mRNA isoforms
Zhu et al., Immunol Lett 2006 : The results from the systems showed that all the three mAbs could neutralize TNF mediated cytotoxicity in L929 cells, TNF induced NF-kappaB activation in ECV304 cells, and TNF upregulated ICAM-1 surface expression on ECV304 cells in dose dependent manners
Iseki et al., Biochem Biophys Res Commun 2000 : Pyrrolidine dithiocarbamate inhibits TNF-alpha dependent activation of NF-kappaB by increasing intracellular copper level in human aortic smooth muscle cells ... In the present study, we investigated effects of PDTC and another antioxidant, N-acetyl-l-cysteine (NAC), on TNF-alpha dependent activation of NF-kappaB in human aortic smooth muscle cells ( HASMC ) ... These results indicate that TNF-alpha dependent expression of MCP-1 in HASMC is tightly regulated by NF-kappaB and that intracellular copper level is crucial for the TNF-alpha dependent activation of NF-kappaB in HASMC
Witherow et al., Proc Natl Acad Sci U S A 2004 : Conversely, suppression of beta-arrestin 1, but not beta-arrestin 2, expression by using RNA interference led to a 3-fold increase in tumor necrosis factor stimulated NF-kappaB activity as measured by NF-kappaB mobility-shift analysis
Darwech et al., J Cell Biochem 2009 (Osteolysis) : Further, CC2 and LZ peptides attenuate RANKL- and TNF induced NF-kappaB signaling in bone marrow derived osteoclast precursors ( OCPs )
Secchiero et al., Blood 2008 : OPG promoter functional assays and chromatin immunoprecipitation experiments revealed inhibitory effects of Nutlin-3 on the TNF-alpha induced NF-kappaB DNA binding activity to the OPG promoter
Kim et al., Exp Mol Med 2008 (Asthma...) : The stimulation of lymphoid cells with TNF-alpha , IL-1beta, and LPS resulted in significant ROS production and NF-kappaB activation
Li et al., Antioxid Redox Signal 2009 : Endosomal Nox2 facilitates redox dependent induction of NF-kappaB by TNF-alpha ... Cellular stimulation by TNF-alpha induced NADPH dependent superoxide production in the endosomal compartment, and this ROS was required for IKK mediated activation of NF-kappaB ... Inhibiting endocytosis reduced the ability of TNF-alpha to induce both NADPH dependent endosomal superoxide and NF-kappaB , supporting the notion that redox dependent signaling of the receptor occurs in the endosome
Albrecht et al., FEBS Lett 1994 : Bcl-2 protects from oxidative damage and apoptotic cell death without interfering with activation of NF-kappa B by TNF ... However, Bcl-2 had no effect on the activation of NF-kappa B by TNF , even though it protected cells from TNF induced apoptosis
Martinesi et al., Cell Biol Int 2006 : Neither apoptosis nor nuclear translocation of NF-kappaB , induced in HUVEC by TNF-alpha was influenced by 1,25 ( OH ) ( 2 ) D ( 3 ) treatment
Huang et al., Molecules (Basel, Switzerland) 2007 : Compounds 1-6 displayed moderate inhibition of tumor necrosis factor alpha (TNF-alpha) induced nuclear factor-kappaB (NF-kappaB) activation, while 2 and 4 displayed potential inhibitory activity toward proteasome chymotripsin-like activation
Wong et al., Shock 2002 (Hyperoxia...) : Hyperoxia prolongs tumor necrosis factor-alpha mediated activation of NF-kappaB : role of IkappaB kinase ... Stimulation with TNFalpha alone increased NF-kappaB activation within 1 h and induced IkappaB alpha degradation within 0.5 h ... These data demonstrate that while hyperoxia alone does not affect activation of NF-kappaB, hyperoxia prolongs TNFalpha mediated activation of NF-kappaB
Morrison et al., Virology 2004 : In spite of this BZLF1 associated increase in the nuclear form of NF-kappaB, BZLF1 did not induce binding of NF-kappaB to NF-kappaB responsive promoters ( as determined by chromatin immunoprecipitation assay ) in vivo, although TNF-alpha treatment induced NF-kappaB binding as expected
Nakano et al., Proc Natl Acad Sci U S A 1999 : Activation of either NF-kappaB or c-Jun NH ( 2 ) -terminal kinase/stress activated protein kinase by tumor necrosis factor , CD27, and CD40 was not abrogated in traf5 ( -/- ) mice
Kang et al., J Pharmacol Exp Ther 2002 : Silymarin also inhibited tumor necrosis factor-alpha induced NF-kappaB/Rel activation, whereas okadaic acid induced NF-kappaB/Rel activation was not affected
Dirsch et al., Eur J Nutr 2004 : This study addresses the question whether the garlic metabolites diallyldisulfide ( DADS ) and allylmercaptane ( AM ) influence the TNF-alpha induced activation of NF-kappaB and the NF-kappaB regulated endothelial gene product E-selectin in human umbilical endothelial cells ( HUVECs )
Aizawa et al., J Biol Chem 1997 : Tumor necrosis factor receptor associated factor ( TRAF) 5 and TRAF2 are involved in CD30 mediated NFkappaB activation
Lerner-Marmarosh et al., Arterioscler Thromb Vasc Biol 2003 (Inflammation) : Human umbilical vein endothelial cells preexposed to flow showed decreased tumor necrosis factor (TNF)-alpha induced c-Jun and NF-kappaB transcriptional activation ... Because MEKK3 and Gab1 are critical for TNF-alpha induced c-Jun and NF-kappaB activation, we determined the role of SHP-2 phosphatase activity in MEKK3 signaling
Ganji et al., Atherosclerosis 2009 : Niacin inhibited : ( a ) angiotensin II (ANG II) induced reactive oxygen species ( ROS ) production by 24-86 %, ( b ) low density lipoprotein (LDL) oxidation by 60 %, ( c ) tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB activation by 46 %, vascular cell adhesion molecule-1 ( VCAM-1 ) by 77-93 %, monocyte chemotactic protein-1 (MCP-1) secretion by 34-124 %, and ( d ) in a functional assay TNF-alpha induced monocyte adhesion to HAEC ( 41-54 % )
Lee et al., Int Immunopharmacol 2008 : Alpha-lipoic acid inhibits TNF-alpha induced NF-kappa B activation through blocking of MEKK1-MKK4-IKK signaling cascades
Yang et al., Biol Pharm Bull 2008 : Moreover, TNF-alpha production was prevented by NF-kappaB and mitogen activated protein kinase ( MAPK ) inhibitors
Peters et al., Mol Cell 2000 : A dominant negative mutant of IKKepsilon blocks induction of NF-kappaB by both PMA and activation of the T cell receptor but has no effect on the activation of NF-KB by TNFalpha or IL-1
Umezawa et al., Mol Cells 2002 : The designed compound, dehydroxymethyl-epoxyquinomicin ( DHMEQ ), inhibited the TNF-alpha induced activation of NF-kappaB , and showed an anti-arthritic effect in mice ... DHMEQ inhibited the TNF-alpha induced cellular DNA binding of nuclear NF-kappaB , but not the phosphorylation or degradation of I-kappaB
Park et al., Cytokine 2002 : Together, the p43 induced TNF production was controlled by NFkB , p38 MAPK, and ERK that is dependent on the activities of PLC and PKC
Adli et al., PloS one 2010 : Analysis of NF-kappaB activation in response to TNF in MEFs reveals that IKKbeta is essential for efficient phosphorylation and subsequent degradation of IkappaB alpha, yet IKKalpha contributes to the NF-kappaB activation response in these cells as measured via DNA binding assays
Watanabe et al., Cell Signal 1999 : However, PDTC did not inhibit tumour necrosis factor-alpha (TNF-alpha) induced NF-kappaB DNA binding activity but potentiated the effect of TNF-alpha on kappaB dependent gene expression
Choi et al., Int Immunopharmacol 2006 : LY29 treatment also augmented tumor necrosis factor (TNF) mediated NF-kappaB DNA binding
Yorek et al., Biochem J 1998 : TNF-alpha activates nuclear factor kappaB (NF-kappaB) in 3T3-L1 adipocytes but, unlike the effect of TNF-alpha on cultured endothelial cells, NF-kappaB does not seem to contribute to the regulation by TNF-alpha of SMIT gene expression in 3T3-L1 adipocytes
Frost et al., Toxicol Appl Pharmacol 2001 : Nuclear factor-kappaB (NF-kappaB) tightly regulates TNF-alpha transcription
Chun et al., Mol Cells 2008 : TNF-alpha induced NF-kappaB activation was examined using these mutants and only zS4 was found to stimulate activation ... Collectively, the results indicate that a spliced form of ZNF268 lacking the KRAB domain is located in the cytosol, where it seems to play a role in TNF-alpha induced NF-kappaB activation by interacting with the IKK complex
Hotta et al., Eur J Pharmacol 2001 : In circulating blood neutrophils, TNF-alpha induced activation of nuclear factor-kappa B (NF-kappa B) , whereas, in tissue neutrophils, NF-kappa B had been already activated without any stimulation, and no further activation was induced by the treatment with TNF-alpha
Haudek et al., Am J Physiol Heart Circ Physiol 2001 (Cardiomyopathies...) : Inhibition of myocyte NF-kappaB activation by overexpression of myocyte I-kappaBalpha is sufficient to block cardiac TNF-alpha production and prevent cardiac dysfunction after LPS challenge
Jana et al., J Neurochem 2002 : However, CD40 ligation alone in the presence or absence of CD40 overexpression induced the activation of only NF-kappaB and not that of C/EBPbeta, suggesting that the activation of NF-kappaB alone by CD40 ligation is not sufficient to induce the expression of TNF-alpha and that the activation of C/EBPbeta is also necessary for strong induction of TNF-alpha ... Taken together, these studies suggest that activation of both NF-kappaB and C/EBPbeta is important for strong induction of TNF-alpha and that CD40 ligation regulates the expression of TNF-alpha by modulating the activation of only NF-kappaB but not that of C/EBPbeta
Ji et al., J Autoimmun 2001 (Arthritis, Rheumatoid...) : Both troglitazone and 15d-PGJ ( 2 ) markedly inhibited TNF-alpha induced NF-kappaB activation at 30 microM
Steiner et al., Eur Urol 2001 (Carcinoma, Renal Cell) : NF-kB signaling induced by TNFalpha is suppressed by IFNalpha and IFNgamma in the permanent cell lines and in the primary RCC tumor cell cultures ... In an in vitro ICT model we show that pretreatment of RCC with IL-2 and IFNalpha leads to a diminished NF-kB response to TNFalpha
Osawa et al., J Immunol 2001 : Although Akt has been reported to activate NF-kappaB, DMS and LY 294002 failed to prevent TNF-alpha induced NF-kappaB activation , suggesting that the antiapoptotic effects of SphK and Akt are independent of NF-kappaB
Zhang et al., Cell Death Differ 2001 (MAP Kinase Signaling System) : Neither U0126 nor LY294002 pretreatment affected TNF induced activation of NF-kappaB , suggesting that the MAP kinase or PI3-kinase/Akt mediated anti-apoptotic effect induced by TNF was not relevant to NF-kappaB activation
Jennings et al., Am Surg 2004 : Nuclear factor-kappaB (NF-kappaB) regulates the expression of TNF ... Because NF-kappaB is activated by both LPS and ROS, we hypothesized that an inhibitor of NF-kappaB , pyrrolidine dithiocarbamate ( PDTC ), would block release of TNF from the heart stimulated by these two agents
Yang et al., J Biol Chem 2003 (Carcinoma, Hepatocellular) : Tumor necrosis factor alpha (TNFalpha) , which activates both NF-kappa B and AP-1, increased MAT2A expression in a dose- and time dependent manner, binding of both NF-kappa B and AP-1 to the MAT2A promoter and MAT2A promoter activity, with the latter effect blocked by site directed mutagenesis of the NF-kappa B and AP-1 binding sites ... Although blocking NF-kappa B had no influence on the ability of TNF alpha to increase AP-1 nuclear binding, blocking AP-1 with dominant negative c-Jun prevented the TNF alpha mediated increase in NF-kappa B binding
Vallée et al., Biochem Biophys Res Commun 2003 (Adenocarcinoma...) : Indeed, TNF alpha induced NF-kappa B translocation was not sufficient to support enhancement of the transcription and des-IGF-1 did not promote but partly inhibited both the TNF alpha induced NF-kappa B activation and I kappa B alpha degradation through a PI-3K dependent pathway
Arntz et al., Arthritis Res Ther 2010 (Arthritis, Experimental...) : In vitro, HpTNFR1 reduced the TNFR1 mRNA expression by three-fold resulting in a 70 % reduction of TNFalpha induced NF-kappaB activation
Dhingra et al., Cardiovasc Res 2009 : IL-10 attenuates TNF-alpha induced NF kappaB pathway activation and cardiomyocyte apoptosis ... Inhibition of ERK 1/2 MAPK with PD98059 attenuated the protective role of IL-10 against TNF-alpha induced activation of IKK and NF kappaB as well as cardiomyocyte apoptosis ... IL-10 prevents TNF-alpha induced NF kappaB activation and pro-apoptotic changes in cardiomyocytes by inhibiting IKK phosphorylation through the activation of ERK 1/2 MAPK
Birkenkamp et al., Leukemia 2004 (Acute Disease...) : The constitutive NF-kappaB activity could so far not be ascribed to the autocrine production of growth factors or to mutations in the Flt3 receptor, since anti-GM-CSF, -IL-1, -IL6, -TNFalpha or the tyrosine kinase inhibitor AG1296 did not affect the NF-kappaB DNA binding activity
Miyamoto et al., J Endod 2005 : The result of EMSA showed that capsaicin inhibited tumor necrosis factor-alpha (TNF-alpha) induced nuclear factor-kappa B (NF-kappaB) activation in PF-10 cell cultures
Ohori et al., Auris Nasus Larynx 2007 (Nasal Polyps) : TNF-alpha upregulates VCAM-1 and NF-kappaB in fibroblasts from nasal polyps ... The activation of NF-kappaB induced by TNF-alpha was determined by electrophoretic mobility shift assays and the influence on the expression of VCAM-1 was investigated ... NF-kappaB activity was enhanced by TNF-alpha stimulation and remarkably suppressed by NF-kappaB proteasome inhibitor
Hipp et al., Eur J Immunol 2002 : Inhibition of the proteasome by MG-132 or lactacystin completely blocked TNF-alpha induced IkappaBalpha degradation as well as NF-kappaB activity in human arterial endothelial cells
Clohisy et al., J Orthop Res 2002 (Osteolysis...) : The nuclear transcription factor NF-kappaB mediates TNF signaling and this transcription complex is necessary for osteoclastogenesis
Deshpande et al., FASEB J 2000 : Expression of Rac1N17 blocked TNF induced activation of nuclear factor-kappa B (NF-kappaB) , increased activity of caspase-3, and markedly augmented endothelial cell susceptibility to TNF induced apoptosis
Xu et al., J Biol Chem 2004 : The tumor necrosis factor receptor associated factor ( TRAF ) protein family members are critically involved in activation of NF-kappaB , JNK, and p38 activation triggered by tumor necrosis factor (TNF) receptor family members and toll/interleukin-1 receptor ( TIR ) -containing receptors
Newman et al., J Surg Res 1998 (Heart Diseases) : Release of tumor necrosis factor-alpha from coronary smooth muscle : activation of NF-kappaB and inhibition by elevated cyclic AMP
Vallejo et al., J Immunol 2000 (MAP Kinase Signaling System) : These results indicate that both NF-kappaB and AP-1 ( via p38 MAPK ) are involved in the regulation of TNF-alpha production in GBS stimulated neonatal monocytes
Rippe et al., DNA Cell Biol 1999 (Fibrosis...) : Fibrosis is frequently associated with inflammation, which is accompanied by increased levels of tumor necrosis factor-alpha (TNFalpha) and activation of the transcription factor NF-kappaB
Shibuya et al., Biochem Biophys Res Commun 2002 : PLL-g-HA/NF-kappaB31, but not control oligodeoxynucleotides having a reverse or scrambled sequence, inhibited the intranuclear localization of NF-kappaB induced by TNFalpha , with almost complete inhibition at 2.5microg/mL as DNA
Liu et al., J Neurosci 2000 : However, pretreatment with oATP downregulated activation of NF-kappaB and AP-1 by IL-1beta or TNFalpha
Chiao et al., AIDS Res Hum Retroviruses 2001 : Tumor necrosis factor receptor (TNFR) p55 was the prominent receptor, as neutralizing antibodies to TNFR p55, but not to TNFR p75, blocked TNF-alpha mediated NF-kappa B activation
Lehmann et al., J Rheumatol 2002 (Osteoarthritis) : We determined the effect of glucocorticoids and tumor necrosis factor (TNF) on nuclear localization and DNA binding of the transcription factor NF-kappaB in osteoarthritic ( OA ) synovial tissue ... TNF induced NF-kappaB nuclear translocation and DNA binding in all OA synovial tissue explants, although there were no consistent effects on AP-1 DNA binding ... Dexamethasone partially inhibits TNF induced NF-kappaB DNA binding in human synovial tissue
Benard et al., PloS one 2010 (Colitis) : Degraded carrageenan causing colitis in rats induces TNF secretion and ICAM-1 upregulation in monocytes through NF-kappaB activation
Gukovskaya et al., J Clin Invest 1997 (Disease Models, Animal...) : TNFalpha potentiated NF-kappaB translocation into the nucleus and stimulated apoptosis in isolated acini while not affecting LDH release
Wang et al., Cancer Res 2006 (Lung Neoplasms) : Treatment with 17AAG caused degradation of RIP and IKKbeta that, in turn, blocked TNF induced NF-kappaB activation and antiapoptotic gene expression ... These results suggest that the cytotoxicity seen with 17AAG and TNF treatment results from blocking TNF induced NF-kappaB activation
Zhang et al., J Nutr 2005 : LGG reduced the TNFalpha induced NFkappaB translocation to the nucleus and lessened the decrease in IkappaB in the cytoplasm ( P < 0.05 )
Jäättelä et al., Oncogene 1995 (Breast Neoplasms) : Interestingly, exposure of MCF-Fas cells to anti-Fas or TNF induced activation of phospholipase A2 (PLA2), while only TNF activated NF-kappa B
Rensing-Ehl et al., J Inflamm 1995 : Furthermore, when equitoxic concentrations of anti-Fas antibody and TNF were applied, TNF triggered a stronger NF-kappa B response
Gustin et al., J Biol Chem 2004 : This study investigated the significance of PI 3-kinase/Akt signaling to tumor necrosis factor (TNF) induced NF-kappa B activation in transformed, immortalized, and primary cells ... Pharmacological inhibition of PI 3-kinase blocked TNF induced NF-kappa B DNA binding in the 293 line of embryonic kidney cells, partially affected binding in MCF-7 breast cancer cells, HeLa and ME-180 cervical carcinoma cells, and NIH 3T3 cells but was without significant effect in H1299 and human umbilical vein endothelial cells, cell types in which TNF activated Akt
Iwamura et al., Genes Cells 2001 : Although the induction of NF-kappaB by dsRNA/virus and TNF-alpha involves common downstream pathways including IKK activation, the upstream, Geldanamycin-sensitive process was unique to the dsRNA/virus induced signal
Ahn et al., J Biol Chem 2006 : TNF induced nuclear factor (NF)-kappaB activation in wild-type but not in NQO1 deleted cells ... The treatment of wild-type cells with dicoumarol, a known inhibitor of NQO1, also abolished TNF induced NF-kappaB activation ... We also found that TNF activated NQO1, and NQO1-specific small interfering RNA abolished the TNF induced NQO1 activity and NF-kappaB activation
Bi et al., Zhonghua Yi Xue Za Zhi 2008 (Diabetes Mellitus, Experimental...) : In the DM rats the IkappaBalpha protein expression in the skeletal muscle was significantly lower ( P < 0.05 ) and the NF-kappaB P65 DNA binding activity increased, and TNF-alpha , IL-1B, and IL-6 expression levels were up-regulated in comparison with the normal control group
Ding et al., J Biol Chem 1998 : A new quantitative cytometric technique, termed the ArrayScanTM, is described and used to measure NF-kappaB nuclear translocation induced by interleukin (IL)-1 and tumor necrosis factor-alpha (TNFalpha)
Vega et al., Biochem Pharmacol 2005 : We also observed that tropisetron is a potent inhibitor of PMA plus ionomycin induced NF-(kappa)B activation but in contrast TNF(alpha) mediated NF-(kappa)B activation was not affected by this antagonist
Sheriff et al., Mol Cell Endocrinol 2012 (Burns...) : DAG also reversed or attenuated the TNF+IFN induced reduction in phosphorylation of Akt, FOXO1, 4E-BP-1 , and GSK-3ß in myotubes
Xie et al., Clin Hemorheol Microcirc 2010 : We further examined the molecular mechanisms and found that the combination of Sal B and LSS treatment dramatically inhibited TNF-alpha induced NF-kappaB activation evidenced by IkappaBalpha degradation and p65 nuclear translocation in HAECs
Rho et al., Toxicol Appl Pharmacol 2007 : Palmitate inhibited an increase of TNF-alpha mediated nuclear factor kappa B (NF-kappaB) activity, the survival pathway activated by the cytokine, by hindering the translocation of the NF-kappaB subunit of p65 from the cytosol into the nucleus
Lou et al., J Biol Chem 2007 : Here we demonstrate in the same system that GSH depletion down-regulates TNF induced NF-kappaB transactivation via two mechanisms, depending on the extent of the depletion ... With moderate GSH depletion ( approximately 50 % ), the down-regulation is IkappaB kinase (IKK) independent and likely acts on NF-kappaB transcriptional activity because TNF induced IKK activation, IkappaBalpha phosphorylation and degradation, NF-kappaB nuclear translocation, NF-kappaB DNA binding in vitro, and NF-kappaB subunit RelA ( p65 ) recruitment to kappaB sites of target gene promoters all appear unaltered
Singh et al., J Immunol 1996 (Leukemia, Myelomonocytic, Acute) : The pretreatment of human myeloid ML-1a cells with capsaicin blocked TNF mediated activation of NF-kappa B in a dose- and time dependent manner
Kil et al., Phytother Res 2008 : In addition, AGR decreased TNF-alpha induced activation of NF-kappaB
Peppel et al., Cardiovasc Res 2005 (Graft Occlusion, Vascular...) : However, only TNF activated NF kappa B
Wang et al., J Huazhong Univ Sci Technolog Med Sci 2005 (Shock, Septic) : 1 h, 2 h, 4 h and 6 h after LPS injection, the activation of NF-kappaB in blood mononuclear cells and the content of TNF-alpha and IL-6 in plasma was detected by enzyme linked immunoadsordent assay ( ELISA )
Amigó et al., Life Sci 2008 (Disease Models, Animal...) : Avarol inhibited tumor necrosis factor-alpha (TNF-alpha) generation in stimulated human monocytes ( IC ( 50 ) 1 microM ) and TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB)-DNA binding in keratinocytes
Kamat et al., Mol Cancer Ther 2007 (Urinary Bladder Neoplasms) : Both gemcitabine and TNF activated NF-kappaB in bladder cancer cells and curcumin suppressed this activation
Popkin et al., J Virol 2003 : MCMV infection blocked TNF-alpha induced nuclear translocation of NF-kappaB
Zamorano et al., Int Immunol 2001 : IL-4 enhanced TNF-alpha induced activation of the transcription factor NF-kappaB
Huang et al., Biochem Biophys Res Commun 2003 : Identification of a novel serine/threonine kinase that inhibits TNF induced NF-kappaB activation and p53 induced transcription
Fotin-Mleczek et al., J Cell Sci 2002 (Rhabdomyosarcoma) : When both TNF-receptors were stimulated simultaneously, TNF-R1 induced NF-kappaB activation remained unaffected but TNF-R1 induced apoptosis was still significantly enhanced
Cooke et al., Biochem J 2001 : However, an IRAK-1 variant lacking only the N-terminal domain retained the ability of the full-length protein to potentiate both IL-1 beta and tumour necrosis factor alpha (TNF alpha) induced NF-kappa B activation ... Expression of an IRAK-1 variant lacking only the C-terminal domain preferentially inhibited IL-1 beta versus TNF alpha induced NF-kappa B activation
Anrather et al., J Immunol 1997 : Similar potentiation of TNF induced NF-kappaB activity and E-selectin transcription by thrombin was observed in experiments utilizing luciferase reporter constructs expressed in bovine aortic EC
Cominacini et al., Am J Hypertens 2002 : Ox-LDL and TNF-alpha increased the activation of NF-kappaB and the preincubation of HUVECs with zofenoprilat, but not with enalaprilat, dose dependently reduced its activation ( P < .001 )
Farivar et al., J Biol Chem 1996 : Tumor necrosis factor-alpha and interferon-gamma induced NF-kappaB ( p50/p65 ) and STAT-1, respectively, as assessed by gel shift assays
Chengedza et al., Anticancer Drugs 2010 (Ovarian Neoplasms) : SHetA2 inhibited basal and TNF-alpha induced or hydrogen peroxide induced NF-kappaB activity through counter-regulation of upstream kinase ( IkappaB kinase ) activity, inhibitor protein ( IkappaB-alpha ) phosphorylation, and p-65 NF-kappaB subunit nuclear translocation, but independently of reactive oxygen species generation
Voisard et al., BMC molecular biology 2001 : We hypothesized that tumor necrosis factor-alpha (TNF-alpha) induced and NF-kappaB mediated expression of intercellular adhesion molecule-1 ( ICAM-1 ) can be inhibited by antisense RelA p65 and NF-kappaB1 p50 oligonucleotides ( RelA p65 and NF-kappaB1 p50 )
Zhang et al., J Clin Endocrinol Metab 2001 : TNF alpha increased the messenger ribonucleic acid levels of ICE, TNF alpha, IL-1 beta, bcl-2, and NF kappa B in preadipocytes and adipocytes ( P < 0.01 )
van Puijenbroek et al., Cytokine 1999 : Phospholipase A2 inhibitors and leukotriene synthesis inhibitors block TNF induced NF-kappaB activation ... The role, however, of AA and its metabolites in TNF induced NF-kappaB activation is still obscure
Fan et al., J Biol Chem 2009 : Here we report that tumor necrosis factor-alpha (TNFalpha) induces RelA polyubiquitination at the lysine 195 residue, and this ubiquitination event is critical for the degradation of RelA and termination of TNFalpha mediated NF-kappaB activation ... Our finding is the first report that substitution of a key RelA lysine residue with arginine inhibits TNFalpha induced RelA ubiquitination and enhances TNFalpha induced NF-kappaB activation
Saldeen et al., Autoimmunity 2000 : TNF-alpha activated NF-kappaB in gel shift experiments without inducing iNOS -- as assessed by nitrite formation -- whereas IL-1beta stimulated both NF-kappaB activation and iNOS induction
Atwood et al., AIDS Res Hum Retroviruses 1994 : Our results suggest that TNF-alpha stimulation of HIV-1 gene expression in primary cultures of human fetal glial cells is mediated by an increase in binding of NF-kappa B ( p50/p65 ) to the HIV-1 LTR
Dalen et al., Br J Cancer 2003 (Lymphoma) : Activation of NF-kappaB by TNF-alpha prior to TRAIL exposure increased resistance of the cells to TRAIL mediated apoptosis
Jackson-Bernitsas et al., Oncogene 2007 (Carcinoma, Squamous Cell...) : The expression of constitutively active NF-kappaB in HNSCC is mediated through the tumor necrosis factor (TNF) signaling pathway, as NF-kappaB reporter activity was inhibited by DN-TNF receptor associated death domain ( TRADD ), DN-TNF receptor associated factor (TRAF)2, DN-receptor interacting protein ( RIP ), DN-transforming growth factor-beta activated kinase 1 ( TAK1 ), DN-kappa-Ras, DN-AKT and DN-IKK but not by DN-TRAF5 or DN-TRAF6
Kaiser et al., Gastroenterology 1999 : TNF-alpha stimulated NF-kappaB activation and nuclear translocation and the degradation of Ikappa-Balpha were blocked by mesalamine
Xue et al., Arthritis Rheum 2007 (Arthritis, Rheumatoid...) : Furthermore, APC directly suppressed the production of tumor necrosis factor (TNF) and the activation of NF-kappaB and MAP kinase p38, and inhibitors of NF-kappaB or p38 reduced the production of MMP-9, suggesting that APC inhibits MMP-9 by blocking TNF, NF-kappaB, and p38
Hippenstiel et al., Biochem Pharmacol 2002 : In order to analyze the role of Rho proteins in TNF-alpha induced NF-kappaB-activation in human umbilical cord vein endothelial cells ( HUVEC ) we used Clostridium difficile toxin B-10463 ( TcdB-10463 ) which inactivates RhoA/Rac1/Cdc42 by glucosylation and Clostridium botulinum C3-toxin which inhibits RhoA/B/C by ADP-ribosylation
Nakano et al., Proc Natl Acad Sci U S A 1998 : A previously identified NF-kappaB inducing kinase (NIK), which mediates NF-kappaB activation by TNFalpha and IL-1, has been demonstrated to activate IKKalpha
Bai et al., Biochem Biophys Res Commun 2007 (MAP Kinase Signaling System) : Rosiglitazone attenuates NF-kappaB dependent ICAM-1 and TNF-alpha production caused by homocysteine via inhibiting ERK1/2/p38MAPK activation
Kajita et al., Diabetes Res Clin Pract 2004 : Furthermore, TNFalpha activated nuclear factor-kappaB (NF-kappaB) , known as a downstream effector of PKCzeta to 256.6 %, which was enhanced with overexpression of wild-type PKCzeta
Márquez et al., Mol Pharmacol 2006 : Arvanil also prevents tumor necrosis factor-alpha induced nuclear factor-kappaB (NF-kappaB) activation by direct inhibition of IkappaBalpha degradation, NF-kappaB binding to DNA, and NF-kappaB dependent transcription
Carpenter et al., BMC infectious diseases 2002 (Lung Neoplasms) : Respiratory syncytial virus and TNF alpha induction of chemokine gene expression involves differential activation of Rel A and NF-kappa B1 ... DNA binding studies using NF-kappaB subunit specific binding ELISA demonstrated that RSV and TNFalpha induced different NF-kappaB binding complexes containing Rel A ( p65 ) and NF-kappaB1 ( p50 )
Chae et al., Immunopharmacol Immunotoxicol 2004 : TNF-alpha plus IFN-gamma can induce extracellular signal regulated kinase ( ERK ), IKK, IkappaB degradation and NF-kappaB activation
Young et al., Bone 2010 : Activation of NF-kappaB by fluid shear stress, but not TNF-alpha , requires focal adhesion kinase in osteoblasts ... We examined the role of a key component of focal adhesions and of mechanotransduction, focal adhesion kinase ( FAK ) in regulation of FSS- and tumor necrosis factor-alpha (TNF-alpha) induced activation of nuclear factor-kappa B (NF-kappaB) signaling in osteoblasts ... Interestingly, FAK was not required for TNF-alpha induced NF-kappaB activation in osteoblasts
Sakaguchi et al., Hepatology 1999 (Drug-Induced Liver Injury...) : The aim of this study is to investigate how PAF participates in the LPS induced and NF-kappaB mediated regulation of TNF-alpha and CINC in regenerating rat livers
Hirota et al., Biochem Biophys Res Commun 2000 : We demonstrated here that intracellular localization of these redox molecules differ from each other and that the redox molecules differentially regulate NF-kappaB , AP-1, and CREB activation induced by TNFalpha , PMA, and forskolin and by expression of signaling intermediate kinases, NIK, MEKK, and PKA in HEK293 cells
Tse et al., Biochem Pharmacol 2005 : Honokiol inhibits TNF-alpha stimulated NF-kappaB activation and NF-kappaB regulated gene expression through suppression of IKK activation ... We observed that the tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activation was blocked by honokiol in four different cancer cell lines as evidenced by EMSA
Bates et al., Exp Cell Res 2004 (Colonic Neoplasms...) : Using a novel model of EMT in colon carcinoma in which the inflammatory cytokine TNF-alpha accelerates this TGF-beta directed process, we report that TNF-alpha stimulation upregulates expression of the chemokine IL-8, and that this upregulation is dependent on the transcription factor NF-kappaB
Novitskiy et al., Alcohol Alcohol 2005 : NF-kappaB , an inhibitor of the type I collagen promoters, is increased by both acetaldehyde and TNFalpha ... This study determined the effects of acetaldehyde in comparison to the effects of TNFalpha on inhibitory kappa B-alpha ( IkappaB-alpha ) protein and NF-kappaB activation in hepatic stellate cells ... Both acetaldehyde and TNFalpha enhanced nuclear NF-kappaB ( p65 ), but acetaldehyde alone also increased NF-kappaB ( p50 ) ... TNFalpha and acetaldehyde independently activate NF-kappaB by rapid enhancement of IkappaB-alpha kinase activity and degradation of IkB-alpha protein
Albrecht et al., J Inflamm 1995 : However, inhibition of TNF-alpha mRNA accumulation by cAMP inducers had no effect on NF-kappa B induction ... We conclude that NF-kappa B translocation is necessary, but not sufficient for the transcriptional induction of the TNF-alpha gene by LPS in macrophages or by phorbol ester and ionomycin in T lymphocytes
Frelin et al., Oncogene 2003 (Leukemia, T-Cell) : Pharmacological blockade of the IKK2 kinase with AS602868, a specific inhibitor that competes with ATP binding, prevented TNF-alpha induced NF-kappaB activation in Jurkat leukemic T cells
Cowland et al., J Immunol 2003 : TNF-alpha activation of NF-kappaB , in contrast, did not increase NGAL synthesis, even though induced binding of NF-kappaB to the NGAL promoter was observed in vitro
Maus et al., Antisense Nucleic Acid Drug Dev 1999 : These DOTMA/DOPE induced changes were not due to alterations in VCAM-1 mRNA stability, nor did DOTMA/DOPE inhibit TNF-alpha induced NF-kappaB-like binding activity in nuclear extracts of HPAEC, as analyzed by electrophoretic mobility shift assay
Jun et al., Inflamm Bowel Dis 2006 (Body Weight...) : Pretreatment of human epithelial HT-29 cells with gliotoxin significantly blocked the I-kappaB degradation and NF-kappaB p65 nuclear translocation induced by tumor necrosis factor-alpha or IL-1beta ; these were parallel with the inhibition of IL-8 secretion and intercellular adhesion molecule-1 expression in the same cells
Lo et al., J Surg Res 1997 : Inhibition of NF kappa B activity by these agents also prevented TNF mRNA expression and TNF production induced by LPS
Ciesielski et al., Eur J Immunol 2002 : As chemokines are important for the progress of an inflammatory response by the recruitment of immuno-competent cells, the role NF-kappaB plays in TNFalpha- or lipopolysaccharides (LPS) induced chemokine secretion by human monocyte derived macrophages was examined
Rabi et al., Mol Carcinog 2008 (Prostatic Neoplasms) : Betulinic acid suppresses constitutive and TNFalpha induced NF-kappaB activation and induces apoptosis in human prostate carcinoma PC-3 cells ... BetA also inhibited TNFalpha induced activation of NF-kappaB via the IkappaBalpha pathway, thereby sensitizing the cells to TNFalpha induced apoptosis
Rath , Biochim Biophys Acta 2005 (Burkitt Lymphoma) : In order to study the relationship between the constitutively active NF-kappaB and IkappaB-alpha, a dominant negative mutant IkappaB-alpha ( IkappaB-alphaDN ), lacking the N-terminal 36 amino acids required for the activation of NF-kappaB by tumor necrosis factor-alpha (TNF-alpha) , was expressed in the Daudi cells
Csiszar et al., J Appl Physiol 2008 (Cardiovascular Diseases...) : In the present review we summarize recent experimental data suggesting that mitochondrial production of reactive oxygen species, innate immunity, the local TNF-alpha converting enzyme (TACE)-TNF-alpha , and the renin-angiotensin system may underlie NF-kappaB induction and endothelial activation in aged arteries
Dhib-Jalbut et al., J Immunol 1995 : Our studies indicate that MV, TNF-alpha , or PIPC induces NF-kappa B ( p50 and p65 subunits ) binding to positive regulatory domain II in the glioma cell line
Takada et al., J Biol Chem 2004 : We have identified two peptides from the p65 subunit of NF-kappaB ( P1 and P6 were from amino acid residues 271-282 and 525-537, respectively ) that, when linked with a PTD derived from the third helix sequence of antennapedia, inhibited tumor necrosis factor (TNF) induced NF-kappaB activation in vivo
Alfano et al., J Immunol 2001 : Of interest, TNF-alpha mediated activation of the cellular transcription factor NF-kappaB was unaffected by PTX-B
Ashikawa et al., J Immunol 2002 : Piceatannol inhibits TNF induced NF-kappaB activation and NF-kappaB mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation ... The treatment of human myeloid cells with piceatannol suppressed TNF induced DNA binding activity of NF-kappaB ... The effect of piceatannol was not restricted to myeloid cells, as TNF induced NF-kappaB activation was also suppressed in lymphocyte and epithelial cells
Jiang et al., Life Sci 2004 : Effects of antioxidants and nitric oxide on TNF-alpha induced adhesion molecule expression and NF-kappaB activation in human dermal microvascular endothelial cells ... The mRNA expression of E-selectin, ICAM-1 and VCAM-1, and activation of NF-kappaB induced by TNF-alpha for 2 h were significantly decreased by the above two pretreatments ... To evaluate the role of antioxidants and nitric oxide in modulating inflammatory processes in the skin, we examined the effects of pyrrolidine dithiocarbamate ( PDTC, 0.1 mM ) and spermine NONOate ( Sper-NO, 1 mM ) on adhesion molecule expression and nuclear factor kappa B (NF-kappaB) activation induced by TNF-alpha ( 10 ng/ml ) in cultured human dermal microvascular endothelial cells ( HDMEC )
Tsou et al., Toxicol Appl Pharmacol 2007 : BSO attenuated the TNF-alpha induced nuclear factor-kappaB (NF-kappaB) activation, however, with no detectable effect on AP-1 and its related mitogen activated protein kinases ( MAPKs )
Shareef et al., Cancer Res 2007 (Adenocarcinoma...) : These findings show that the reduced bystander response in A549 cells is due to activation of NF-kappaB signaling by TNF-alpha , whereas enhanced response to IR-induced bystander signaling in H460 cells was due to release of TRAIL associated with nuclear translocation of PAR-4
Johns et al., J Immunol 1994 : Recent studies demonstrate that the sphingomyelinase-ceramide pathway plays a potential role in the activation of nuclear factor-kappa B (NF-kappa B) by TNF-alpha ... This data suggests that although ceramide and 1,2-diacylglycerol ( DAG ) pathways may contribute to TNF-alpha activation of NF-kappa B , impedance of these pathways does not block TNF-alpha from activating NF-kappa B nor induction of the functional activation of the NF-kappa B responsive reporter construct, HCMV
Funakoshi-Tago et al., Int Immunopharmacol 2010 (Edema) : Previously, we reported that a major component, Licochalcone A, potently inhibited TNFalpha induced NF-kappaB activation by inhibiting IKKbeta activation ... Interestingly, reduced Licochalcone A, which lacks a double bond, failed to inhibit TNFalpha induced NF-kappaB activation ... Whereas Licochalcone A potently inhibited TNFalpha induced IKK activation, IkappaBalpha degradation, nuclear localization of NF-kappaB and its DNA binding activity, no inhibitory effect was observed by reduced Licochalcone A
Kim et al., Eur J Pharmacol 2009 (Inflammatory Bowel Diseases) : In addition, M1-M4 significantly suppressed TNF-alpha induced NF-kappaB transcriptional activity ... Treatment of HT-29 cells with M1 and PDTC, a NF-kappaB inhibitor, synergistically suppressed both TNF-alpha induced NF-kappaB activation and monocytic cell adhesion to HT-29 cells
Kouba et al., J Immunol 1999 : These data suggest that in fibroblasts TNF-alpha activates and initiates the nuclear translocation of NF-kappa B that binds a divergent NF-kappa B element and plays a critical role in the observed inhibition of alpha 2 ( I ) collagen gene transcription
May et al., J Biol Chem 2004 : Deletion of the ULD rendered IKKbeta catalytically inactive and unable to induce NF-kappaB activity, and overexpression of only the ULD dose-dependently inhibited tumor necrosis factor-alpha induced NF-kappaB activity
Rao et al., J Biol Chem 2004 (Breast Neoplasms...) : hCG treatment prevented the tumor necrosis factor dependent NF-kappaB and AP-1 activation, which paralleled a decrease in the phosphorylation and degradation of IkappaBalpha
Soler et al., Eur J Cell Biol 1999 : Tumor necrosis factor-alpha (TNF) induces apoptosis in confluent LLC-PK1 epithelial cells, but also activates NF-kappaB , a negative regulator of apoptosis
Hayakawa et al., J Am Soc Nephrol 2010 : Overexpression of C/EBPbeta markedly suppressed TNF-alpha induced activation of NF-kappaB , independent of its transacting potential
Strozyk et al., Oncogene 2006 (Chromosome Breakage) : Surprisingly, activated NF-kappaB induced TNF-alpha mRNA expression in the presence of all DNA damage inducing agents
Stephens et al., J Autoimmun 1997 (Diabetes Mellitus, Type 1) : In the current study we have examined the effect of the same inhibitor of NF-kappaB on class I major histocompatibility complex ( MHC ) protein expression in NIT-1 cells and shown that inhibition of NF-kappaB activation decreased basal and TNF induced class I MHC levels
Seppänen et al., Eur J Endocrinol 2000 (Cystadenocarcinoma...) : The binding activity of AP-1 was found to be stimulated by the growth inhibitory cytokines, TGF-beta1 and TNF-alpha, and the binding of NF-kappaB was stimulated by TNF-alpha
Ichikawa et al., J Ethnopharmacol 2010 : The activation of NF-kappaB by TNF was completely blocked by a Lagerstroemia speciosa extract in a dose- and time dependent manner in H9c2 cells
Quiles-Perez et al., Hepatology 2010 (Carcinoma, Hepatocellular...) : Furthermore, the results obtained in mice treated with DEN to induce hepatocarcinogenesis showed, after treatment with a PARP inhibitor ( DPQ ), a significant reduction both in preneoplastic foci and in the expression of preneoplastic markers and proinflammatory genes ( Gstm3, Vegf, Spp1 [ Opn ], IL6, IL1b, and Tnf ), bromodeoxyuridine incorporation, and NF-kappaB activation in the initial steps of carcinogenesis ( P < 0.05 )
Hu et al., J Biol Chem 2005 : NIBP overexpression potentiates tumor necrosis factor-alpha induced NF-kappaB activation through increased phosphorylation of the IKK complex and its downstream I(kappa)B(alpha) and p65 substrates ... Finally, knockdown of NIBP expression by small interfering RNA reduces tumor necrosis factor-alpha induced NF-kappaB activation , prevents nerve growth factor induced neuronal differentiation, and decreases Bcl-xL gene expression in PC12 cells
Bergmann et al., J Biol Chem 1998 : Two closely related IkappaBalpha kinases as well as the upstream kinase, NIK, which integrates interleukin-1beta (IL-1beta)- and tumor necrosis factor (TNF)-alpha dependent activation of the transcription factor NF-kappaB have recently been described
Hýzd'alová et al., Cytokine 2008 : TNF-alpha induced rapid NF-kappaB activation in both HT-29 and FHC cell lines and this effect was differently modulated by NaBt in these two cell lines ... In HT-29 cells, NaBt potentiated NF-kappaB activity induced by TNF-alpha after 4h treatment ... During additional time of TNF-alpha and NaBt co-treatment, NaBt decreased the TNF-alpha mediated NF-kappaB activity in both cell types
Pang et al., J Biol Chem 1992 (Carcinoma, Papillary...) : TNF-alpha activated NF-kappa B , a nuclear factor thought to mediate multiple actions of TNF-alpha, in these cells with a maximum effect observed after 30 min of treatment
Kim et al., Food Chem Toxicol 2010 (Inflammation) : Further studies revealed that EC extracts suppress the production of tumor necrosis factor-alpha (TNF-alpha) and activation of nuclear factor-kappa B (NF-kappaB)
Cicek et al., Cancer Res 2005 (Breast Neoplasms...) : Further, we showed that suppression of both constitutive and tumor necrosis factor-alpha induced NF-kappaB activation by BRMS1 may be due to inhibition of IkappaBalpha phosphorylation and degradation
Lin et al., Immunity 1999 : Conversely, expression of kinase-deficient Cot inhibits CD3/CD28 but not TNF alpha induction of NF-kappaB
Chung et al., Biol Pharm Bull 2007 : These findings demonstrate that the inhibition of the LPS/rIFN-gamma induced production of NO and TNF-alpha by MC is due to the inhibition of NF-kappaB activation
Menon et al., J Biol Chem 1995 : Inhibitors of phosphotyrosyl protein phosphatases, pervanadate and phenylarsine oxide, abrogate tumor necrosis factor (TNF) induced nuclear factor kappa B (NF-kappa B) nuclear translocation in transformed cell lines ( U-937 and Jurkat ) and primary fibroblasts ( MRC-5 and REF ) ... Treatment of cells with pervanadate inhibited TNF induced I kappa B-alpha phosphorylation and degradation, whereas the serine protease inhibitors tosylphenylalanyl chloromethyl ketone and N alpha-p-tosyl-L-lysine chloromethyl ketone prevented TNF induced I kappa B-alpha degradation and NF-kappa B nuclear translocation, but not the TNF induced phosphorylation of I kappa B-alpha ... The data suggest that TNF and okadaic acid induce the activation of a putative protein-tyrosine phosphatase ( s ), leading to I kappa B-alpha serine phosphorylation and degradation and NF-kappa B nuclear translocation
He et al., Arthritis Rheum 2008 (Arthritis, Experimental...) : Inhibition of ROK by specific inhibitors or ROK small interfering RNA suppressed lipopolysaccharide- or TNFalpha induced NF-kappaB nuclear translocation, DNA binding activity, luciferase reporter gene expression, and IkappaBalpha degradation
Chuang et al., Biochem Pharmacol 2002 (Drug Hypersensitivity) : In contrast to tumor necrosis factor-alpha (TNF-alpha) , which activates NF-kappaB in minutes, NF-kappaB activation induced by anticancer drugs usually occurred more than 1hr after stimulation
Sawaya et al., J Neuroimmune Pharmacol 2009 (HIV Infections) : This study examines the role of NF-kappaB in the regulation of TNFalpha production by morphine in microglia ... The use of a small interfering RNA directed against p65, a subunit of NF-kappaB, demonstrated that TNFalpha induction by morphine is NF-kappaB dependent
Pahlevan et al., J Antimicrob Chemother 2002 : Potential of rifamides to inhibit TNF induced NF-kappaB activation
Peng et al., Zhonghua Gan Zang Bing Za Zhi 2005 (Reperfusion Injury) : Glycine could efficiently protect rat liver grafts from ischemia-reperfusion injury by repressing the expression of CD14 and NF-kappa B binding activity in Kupffer cells and inhibiting the productions of TNF alpha and IL-1
Millán et al., Oncogene 2003 : In this study, the basal and tumor necrosis factor alpha (TNFalpha) induced activation of NF-kappaB has been examined in cells overexpressing H-Ras, K-Ras or N-Ras
Adam et al., Mol Cell Biol 2003 : Potentiation of tumor necrosis factor induced NF-kappa B activation by deacetylase inhibitors is associated with a delayed cytoplasmic reappearance of I kappa B alpha ... Importantly, TSA prolonged both TNF induced DNA binding activity and the presence of NF-kappa B in the nucleus
Hwang et al., Phytother Res 2010 : A further analysis indicated that EGJ attenuated TNF-alpha induced nuclear p65 nuclear factor-kappa B (NF-kappaB) translocation, suggesting that EGJ primarily affects the TNF-alpha induced NF-kappaB signaling pathway ... Taken together, we provided here the first evidence showing that EGJ is able to inhibit TNF-alpha induced NF-kappaB activation , adhesion molecule expression, and monocyte-endothelial interaction, suggesting an anti-inflammatory role of EGJ, which may be useful in preventing vascular diseases, such as atherosclerosis
Wang et al., Biochem Biophys Res Commun 2007 (Lung Neoplasms) : The results show that Akt contributes to TNF induced NF-kappaB activation in lung cancer cells through regulating phosphorylation of the p65/RelA subunit of NF-kappaB ... Although individually blocking IKK or Akt partially suppressed TNF induced NF-kappaB activation, concurrent suppression of these pathways completely inhibited TNF induced NF-kappaB activation and downstream anti-apoptotic gene expression, and synergistically potentiated TNF induced cytotoxicity
Babu et al., Arch Pharm Res 2008 : Taken together, our results suggest that the anti-oxidant components of EPS prevent TNF-alpha induced NF-kappaB activation, chemokine induction, and monocyte adhesion at the site of intestinal inflammation
Prösch et al., J Biol Chem 2001 : We demonstrate here that the cell differentiation dependent reduction of TNFalpha stimulation is not due to insufficient NF-kappaB activation but correlates with increased synthesis of the monocyte differentiation associated factors CCAAT/enhancer binding protein (C/EBP) alpha and beta
Xie et al., Am J Physiol Lung Cell Mol Physiol 2000 : Gel shift and Western blot analyses confirmed that TNF-alpha activated NF-kappaB and depleted IkappaB in this system and that these effects were prevented by MG-132 and pyrrolidine dithiocarbamate
Kim et al., Mol Pharmacol 2006 (Colitis) : TauCl inhibited tumor necrosis factor (TNF) dependent NF-kappaB activation by modifying thiol ( s ) on p65 and blocking DNA binding ... Furthermore, combined TauCl/5-ASA treatment additively inhibited TNF dependent NF-kappaB activation ... Together, our data suggest that the colon-specific carrier taurine contributes to the clinical effect of the prodrug by potentiating the inhibitory effect of the active ingredient 5-ASA on a major proinflammatory signal, TNF dependent NF-kappaB activation in the inflamed large intestine
Sugino et al., J Clin Endocrinol Metab 2002 : Electrophoretic mobility shift assay revealed that TNF alpha ( 1 ng/ml ) and phorbol 12-myristate 13-acetate ( TPA ; 0.4 micro M ), PKC activator, caused marked increases in nuclear NF-kappa B DNA binding activity
Chung et al., Biochem Biophys Res Commun 2001 : Hepatitis C virus core protein potentiates TNF-alpha induced NF-kappaB activation through TRAF2-IKKbeta dependent pathway ... Previous work has implicated that the core protein of hepatitis C virus ( HCV ) may play a modulatory effect on NF-kappaB activation induced by TNF-alpha ... Here we show that overexpression of HCV core protein potentiates NF-kappaB activation induced by TNF-alpha ... In addition, HCV core protein associates with TNF-R1-TRADD-TRAF2 signaling complex, resulting in synergistically activation of NF-kappaB induced by TNF-alpha
Chen et al., Mol Cell Biol 2005 : Reconstitution of RelA-deficient murine embryonic fibroblasts with RelA S276A or RelA S536A decreased TNF-alpha induced acetylation of lysine 310 and expression of the endogenous NF-kappaB-responsive E-selectin gene
Babu et al., Arch Pharm Res 2009 : Moreover, ACK significantly suppressed TNF-alpha induced translocation of redox-sensitive nuclear factor (NF)-kappaB as well as degradation of cytosolic I-kappaBalpha
Yu et al., Curr Biol 1999 : Overexpression of a dominant negative mutant of RIP3 strongly inhibited the caspase activation but not the NFkappaB activation induced by TNFalpha
Weber et al., J Immunol 1995 : Our data suggest that specific phosphorylation following protein tyrosine kinase activation may be required for NF-kappa B mobilization and induction of VCAM-1 and ELAM-1 by TNF
Suzuki et al., Biochem Mol Biol Int 1993 : Vitamin E derivatives have been shown to inhibit tumor necrosis factor induced NF-kappa B activation in human Jurkat T cells
Chen et al., J Vasc Res 2007 (Vasculitis) : Exposure to Ang II ( 10 ( -6 ) M for 24 h ) also enhanced intracellular ROS elaboration and the levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-8, upregulated chemokine receptor CXCR2 mRNA expression, increased adhesion of endothelial cells to monocytes and induced a significant increase in the activity of nuclear factor (NF)-kappaB , which was attenuated by pretreatment with the Ang II receptor blocker losartan ( 1, 3 and 10 muM )
Buzzelli et al., Endocrinology 2008 : We conclude the inhibitory effects of TNF on GH-inducible promoter activity are mediated by NFkappaB , especially p65, by a mechanism that does not require protein synthesis
Gong et al., J Am Soc Nephrol 2004 (Disease Progression...) : HGF also blunted TNF-alpha induced nuclear translocation and activation of NF-kappaB , a pivotal transcription factor that regulates chemokine expression
MacLachlan et al., J Cell Biochem 1998 : Although basal NF-kappaB activity decreases during myogenesis, TNF induced NF-kappaB activity is 10 times greater in myotubes compared with myoblasts, presumably because of the stockpiling of TRAF2 protein in these cells
Jiang et al., Science 1999 : Tumor necrosis factor receptor type 1 ( TNF-R1 ) contains a cytoplasmic death domain that is required for the signaling of TNF activities such as apoptosis and nuclear factor kappa B (NF-kappaB) activation
Jackson et al., Bioorg Med Chem Lett 2002 (Lung Neoplasms) : The compounds display good potency in inhibiting TNF-alpha induced apoptosis and NF kappa B activation
Pohlers et al., Arthritis Res 2002 (Arthritis, Experimental...) : Differential clinical effects at the onset of AA were paralleled by a differential influence of the mAbs on T-cell functions, i.e. in comparison with OX35 and W3/25, the ` accelerating ' mAb RIB5/2 failed to increase the delayed-type hypersentivity ( DTH ) to Mycobacterium tuberculosis, increased the in vitro tumor necrosis factor (TNF)-alpha secretion, and more strongly induced NF-kappaB binding activity after anti-CD4 preincubation and subsequent TCR/CD3-stimulation
Scheper et al., PloS one 2010 : In mammalian cells, they inhibit lysine 63-type polyubiquitylation of PCNA, inhibit activation of NF-kappaB by TNF-alpha and sensitize tumor cells to chemotherapeutic agents
Tsou et al., Toxicol Appl Pharmacol 2005 : Moreover, we found that depletion of GSH would also attenuate the TNF-alpha induced VCAM-1 expression with a down-regulation of the TNF-alpha induced NF-kappaB activation and without significant effect on AP-1
Yadav et al., Biochem Pharmacol 2010 : Using the electrophoretic mobility shift assay, we found that CDC was more active than free curcumin in inhibiting TNF induced activation of the inflammatory transcription factor NF-kappaB and in suppressing gene products regulated by NF-kappaB, including those involved in cell proliferation ( cyclin D1 ), invasion ( MMP-9 ), and angiogenesis ( VEGF )
Mulabagal et al., Phytother Res 2009 : Both S-containing dimeric withanolides, 1 and 2, completely suppressed TNF induced NF-kappaB activation when tested at 100 microm ... In addition, this is the first report on the abrogation of TNF induced NF-kappaB activation for compounds 1 and 2
Rohr et al., Nucleic Acids Res 1999 : However, in contrast with TNF-alpha , dopamine treatment did not affect NF-kappaB DNA binding activity nor the concentrations of p50, p65 and IkappaB-alpha proteins, which suggests a distinct NF-kappaB activation mechanism
Kerbauy et al., Blood 2005 (Myelodysplastic Syndromes) : Exogenous TNF-alpha enhanced NF-kappaB nuclear translocation in MDS BMMCs above baseline levels ... Treatment with arsenic trioxide ( ATO ; 2-200 microM ) inhibited NF-kappaB activity in normal marrow, primary MDS, and ML1 cells, even in the presence of exogenous TNF-alpha ( 20 ng/mL ), and down-regulated NF-kappaB dependent antiapoptotic proteins, B-cell leukemia XL ( Bcl-XL ), Bcl-2, X-linked inhibitor of apoptosis (XIAP), and Fas associated death domain (FADD)-like interleukin-1beta converting enzyme ( FLICE ) inhibitory protein ( FLIP ), leading to apoptosis
Suzuki et al., Biochem Mol Biol Int 1994 (Leukemia, T-Cell) : Both nitecapone and OR-1246 ( 10-300 microM ) inhibited NF-kappa B activation induced by tumor necrosis factor-alpha in Jurkat T ( acute human leukemia ) cells
Clemens et al., Brain Res Mol Brain Res 1997 (Brain Ischemia) : The oxidative stress responsive transcription factor nuclear factor-kappa B (NF-kappa B) consists of a p50 ( 50 kDa ) and p65/RelA ( 65 kDa ) component and can be activated in vitro by TNF alpha , IL1 beta, hydrogen peroxide and oxygen radicals
Higuchi et al., Antioxid Redox Signal 2002 (Leukemia, Myeloid, Acute) : Additionally, tumor necrosis factor (TNF) induced activation of NF-kappaB and AP-1 observed in ML-1a was greatly reduced in clone 19
Wyler et al., Protein Sci 2007 : When expressed in human cells, some of the selected molecules, despite their partial degradation, inhibited TNF-alpha mediated NF-kappaB activation while having no effect on the basal activity
Sohur et al., Cancer Res 2000 : In the progenitor B lymphocyte model FL5.12, whereas NF-kappaB has an antiapoptotic function in response to tumor necrosis factor-alpha , cytokine withdrawal causes nuclear translocation of NF-kappaB/cRel, where it is apoptogenic
Mack et al., Proc Natl Acad Sci U S A 2008 : Here, we show that the murine CMV M45 protein binds to RIP1 and inhibits TNFalpha induced activation of NF-kappaB , p38 MAPK, and caspase independent cell death
Sakurai et al., J Biol Chem 1999 : Furthermore, tumor necrosis factor-alpha activated endogenous TAK1, and the kinase negative TAK1 acted as a dominant negative inhibitor against tumor necrosis factor-alpha induced NF-kappaB activation
Heyninck et al., J Cell Biol 1999 : We show that A20 does not inhibit TNF- induced nuclear translocation and DNA binding of NF-kappaB , although it completely prevents the TNF- induced activation of an NF-kappaB dependent reporter gene, as well as TNF induced IL-6 and granulocyte macrophage-colony stimulating factor gene expression ... Moreover, NF-kappaB activation induced by overexpression of the TNF receptor associated proteins TNF receptor associated death domain protein ( TRADD ), receptor interacting protein ( RIP ), and TNF recep- tor associated factor 2 ( TRAF2 ) was also inhibited by expression of A20, whereas NF-kappaB activation induced by overexpression of NF-kappaB inducing kinase (NIK) or the human T cell leukemia virus type 1 ( HTLV-1 ) Tax was unaffected
Dey et al., Cell Death Differ 2008 : We show in this study that R-Roscovitine can downregulate nuclear factor-kappa B (NF-kappaB) activation in response to tumor necrosis factor (TNF)alpha and interleukin 1
Kanazawa et al., J Bone Miner Res 2005 (Bone Resorption) : Only a few TRACP ( + ) multinucleate cells were formed, and TNF-alpha mediated activation of JNK, NF-kappaB , and NFATc1 was defective
Ishinaga et al., Biochem J 2009 : TGF-beta1 synergistically enhances TNF-alpha induced NF-kappaB DNA binding activity via induction of RelA acetylation
Kettritz et al., J Biol Chem 2004 : In contrast, TNF-alpha activated NF-kappaB both in suspended cells and adherent cells
Ramana et al., Chem Biol Interact 2003 : Stimulation with TNF-alpha led to the activation of the transcription factor NF-kappaB and enhanced VSMC growth
Mazor et al., Cytokine 2000 : Pretreatment with either compound inhibited TNF-alpha mediated activation of the IL-8 promoter and TNF-alpha mediated nuclear translocation of NF-kappaB
Hideshima et al., Semin Oncol 2001 (Multiple Myeloma...) : Tumor necrosis factor-alpha (TNF-alpha), which is produced by some MM cells, induces only low-level MM proliferation and MAPK activation in MM cells, but markedly upregulates IL-6 secretion from BMSCs and upregulates expression of adhesion molecules ( VLA-4 and LFA-1 ) on MM cells and their receptors ( VCAM-1 and ICAM-1 ) on BMSCs, with resultant increased binding of MM cells to BMSCs. Inhibition of TNF-alpha induced NF-kappaB activation with PS-341 inhibits both the upregulation of these molecules on MM cells and BMSCs and the resultant increased adhesion
Yerneni et al., Diabetes 1999 (Hyperglycemia) : The augmented TNF-alpha induced NF-kappaB activation in HG was associated with increased TNF-alpha mediated transcriptional activation of the vascular cell adhesion molecule-1 promoter
Zwart et al., J Bone Miner Res 2010 (Bone Resorption) : We investigated the effects of EPA on differentiation of RAW264.7 monocyte/macrophage cells induced by receptor activator of NF-kappaB ligand ( RANKL ) and on activation of NF-kappaB by tumor necrosis factor alpha (TNF-alpha) or exposure to modeled weightlessness
Wang et al., Diabetes Metab Res Rev 2007 (Diabetes Mellitus, Experimental) : ADMA ( 30 microM ) significantly increased the activity of NF-kappaB and elevated the levels of ICAM-1 and TNF-alpha , and pre-treatment with rosiglitazone ( 10 or 30 microM ) markedly inhibited the increased activity of NF-kappaB and reduced the elevated levels of TNF-alpha and ICAM-1 induced by ADMA in cultured endothelial cells
Hellermann et al., J Allergy Clin Immunol 2004 : Similarly, pNHP ( 73-102 ) decreased TNF-alpha induced NFkappaB activation in NHBE cells
Kohn et al., Shock 2002 : Following heat shock, decreased TNF-alpha induced NF-kappaB activation was observed and was associated with preservation of IkappaB-alpha and a decrease in phosphorylated IkappaB-alpha that correlated to inhibition of I kappa kinase (IKK) activity
Gu et al., J Biol Chem 2006 (Inflammation) : In this study, we report that NF-kappaB activation by lipopolysaccharide and tumor necrosis factor-alpha plays a pivotal role in the suppression of cyp3a4 through interactions of NF-kappaB with the PXR.retinoid X receptor ( RXR ) complex
Clarke et al., Eur J Immunol 1995 : IL-4 was also found partially to inhibit NF kappa B activity induced by tumor necrosis factor-alpha (TNF-alpha) and interleukin-1-beta (IL-1 beta)
Webber et al., Hepatology 1998 : The results demonstrate that TNF transiently activates NF-kappaB and STAT3 and increases the proliferative response of hepatocytes to HGF or TGF- by fourfold
Baur et al., J Endocrinol 2000 : Electrophoretic mobility shift assays ( EMSAs ) revealed that IL-1 beta and TNF alpha activate the transcription factor NF kappa B in reaggregates of rat anterior pituitaries and in TtT/Gf cells cultured alone or cocultured with GH3 cells
Hu et al., Mol Cell Biol 2006 (Breast Neoplasms...) : Further studies suggest that ER stress induces down-regulation of TRAF2 expression, which impairs TNF-alpha induced activation of NF-kappaB and c-Jun N-terminal kinase and turns TNF-alpha from a weak to a powerful apoptosis inducer
Gong et al., J Am Soc Nephrol 2006 (Chronic Disease...) : In addition, HGF suppressed NF-kappaB reporter gene activity that was induced by TNF-alpha and counteracted TNF-alpha elicited NF-kappaB interaction with kappaB elements at the E-selectin gene level
Choi et al., Mol Cell Biol 2006 (Carcinoma, Hepatocellular...) : We recently reported that NS5A protein interacts with TRAF2 and modulates tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB and Jun N-terminal protein kinase (JNK) ... Since NS5A and NS5B are the essential components of the HCV replication complex, we examined whether NS5B could modulate TNF-alpha induced NF-kappaB and JNK activation ... In this study, we have demonstrated that TNF-alpha induced NF-kappaB activation is inhibited by NS5B protein in HEK293 and hepatic cells
Tada et al., J Biol Chem 2001 : Critical roles of TRAF2 and TRAF5 in tumor necrosis factor induced NF-kappa B activation and protection from cell death ... However, the exact roles of TRAF2 and TRAF5 in TNF induced NF-kappaB activation still remain controversial ... Collectively, these results indicate that both TRAF2 and TRAF5 are involved in TNF induced NF-kappaB activation and protection from cell death
Parcellier et al., Mol Cell Biol 2003 : HSP27 overexpression increases NF-kappaB nuclear relocalization, DNA binding, and transcriptional activity induced by etoposide, TNF-alpha , and interleukin 1beta
Huber et al., Digestion 2002 : Western blot analysis revealed that TNF-alpha caused degradation of I kappa B alpha within 10 min. EMSA demonstrated that TNF-alpha led to increased DNA binding activity of NF kappa B and that proteasome inhibitors counteracted NF kappa B activation
Cataisson et al., J Immunol 2005 (Inflammation) : To determine whether TNFalpha mediated NF-kappaB translocation and subsequent expression of proinflammatory factors, K5-PKCalpha mice were treated systemically with a dimeric soluble form of p75 TNFR ( etanercept ) or crossed with mice deficient for both TNFR isoforms, and keratinocytes were cultured in the presence of TNFalpha neutralizing Abs
Kuprash et al., Cancer Lett 2008 (Inflammation...) : TNF via TNFR1 axis induces NFkappaB , and may contribute to inflammation facilitated neoplasia
Reynaert et al., Proc Natl Acad Sci U S A 2006 : Conversely, GRX1 knockdown sensitizes cells to oxidative inactivation of IKK-beta and dampens TNF-alpha induced IKK and NF-kappaB activation
Graham et al., J Biol Chem 2006 : Analysis of differentiating epithelia showed that only well differentiated enterocytes activated the 4-kb long MLCK promoter in response to TNF, and consensus promoter reporters demonstrated that TNF induced NFkappaB activation decreased during differentiation while TNF induced AP-1 activation increased
Zhou et al., Acta Pharmacol Sin 2006 : The transcription and expression of the IkappaBalphaM gene, and the inhibitory effect of IkappaBalphaM on tumor necrosis factor (TNF)-alpha induced NF-kappaB activation in mature DC were detected by polymerase chain reaction ( PCR ), reverse transcription-polymerase chain reaction ( RT-PCR ), Western blot analysis, and electrophoretic mobility shift assays, respectively ... Furthermore, AdIkappaBalphaM significantly suppressed the TNF-alpha induced NF-kappaB activation, augmented apoptosis, downregulated CD80, CD83, and CD86 surface molecules, IL-12 secretion levels and the ability to stimulate the proliferation of T cells in mature DC
Hehner et al., Eur J Biochem 1999 : Pervanadate induced tyrosine phosphorylation increased the TNF-alpha induced cytotoxicity and NF-kappaB activation, suggesting that it stimulates early signaling events prior to the separation of the two signaling pathways
Guo et al., Free Radic Biol Med 2008 : CH3-Avn-c, a synthetically prepared methyl ester derivative of Avn-c with a high biological potency, significantly and dose dependently decreased mRNA expression and secretion of IL-6, IL-8, and MCP-1 by HAEC as determined by real-time RT-PCR and ELISA, and it inhibited IL-1beta- and TNFalpha stimulated NF-kappaB activation as determined by a NF-kappaB DNA binding assay and a NF-kappaB luciferase reporter assay
Yuan et al., World J Gastroenterol 2006 (Liver Cirrhosis, Alcoholic) : The enhanced iNOS activity and expression were positively correlated with the liver damage, especially the necro-inflammation, activation of NF-kappaB , and TNF-alpha mRNA expression
Loftis et al., Am J Physiol Regul Integr Comp Physiol 2000 (Anoxia...) : Here we tested the hypothesis that hypoxic suppression of postbacteremic TNF-alpha gene expression is transcriptionally mediated by reduced activation of NF-kappaB
Li et al., Genes Dev 1999 (Skin Abnormalities) : NF-kappaB activation mediated by TNFalpha and IL-1 is diminished in IKK1-deficient mouse embryonic fibroblast (MEF) cells
Foxwell et al., Proc Natl Acad Sci U S A 1998 (Arthritis, Rheumatoid) : Efficient adenoviral infection with IkappaB alpha reveals that macrophage tumor necrosis factor alpha production in rheumatoid arthritis is NF-kappaB dependent
Yang et al., Mol Immunol 2008 (Escherichia coli Infections...) : Bovine TLR2 and TLR4 properly transduce signals from Staphylococcus aureus and E. coli, but S. aureus fails to both activate NF-kappaB in mammary epithelial cells and to quickly induce TNFalpha and interleukin-8 ( CXCL8 ) expression in the udder
Kasof et al., Oncogene 2001 (Prostatic Neoplasms) : Tumor necrosis factor-alpha induces the expression of DR6, a member of the TNF receptor family, through activation of NF-kappaB
Jeong et al., J Biol Chem 2004 : Depletion of TRP14 augmented the TNF-alpha induced phosphorylation and degradation of I kappa B alpha as well as the consequent activation of NF-kappa B to a greater extent than did Trx1 depletion
Dominiak et al., Pharm Res 2010 (Neoplasms) : The mean NF-kappaB DNA binding activity was increased upon TNF-alpha treatment in pre-intervention samples ; however, TNF-alpha was unable to induce NF-kappaB in post-intervention samples, suggesting that the mixture of four important natural agents could be useful to protect humans against oxidative stress
Park et al., Biochem Biophys Res Commun 2007 (Embryo Loss) : TRAF interacting protein (TRIP) is required for the inhibitory regulation of TNF induced NF-kappaB signaling via the TNFR/TRAF signaling complexes in vitro
Kim et al., Cell Signal 2010 : In this study, we show that KEAP1 is a new IKK binding partner, which is responsible for the down-regulation of TNFalpha stimulated NF-kappaB activation
Chen et al., American journal of physiology. Renal physiology 2009 (Reperfusion Injury) : PPAR-alpha reduced the NF-kappaB induced overexpression of TNF-alpha and apoptosis in cultured kidney cells ... This indicated that PPAR-alpha attenuated renal I/R injury via NF-kappaB induced TNF-alpha overexpression ... Taken together, our results demonstrate for the first time that prostacyclin induces the translocation of PPAR-alpha from the cytosol into the nucleus and attenuates NF-kappaB induced TNF-alpha activation following renal I/R injury
Tomita et al., Gene Ther 2000 (Glomerulonephritis) : The expression of several cytokines and adhesion molecules is regulated by the transcription factor NFkappaB , which is activated by tumor necrosis factor alpha (TNF-alpha)
Figarola et al., Diabetes 2007 (Inflammation) : These inhibitory effects may be exerted via inhibition of nuclear factor-kappaB (NF-kappaB) activation, as LR-90 suppressed both S100b-and tumor necrosis factor-alpha induced IkappaB-alpha degradation as well as NF-kappaB promoter transcriptional activity
Masamune et al., Pancreas 2002 (Acute Disease...) : IL-1 beta and TNF-alpha increased the nuclear factor-kappa B (NF-kappa B)-specific and activator protein-1-specific DNA binding activity, whereas the NF-IL6 activity was not altered
Sangiovanni et al., PloS one 2013 : In vitro, ETs inhibited TNF-a induced NF-kB driven transcription ( IC50 : 0.67-1.73 µg/mL ) and reduced TNF-a induced NF-kB nuclear translocation ( 57 % -67 % at 2 µg/mL )
Kanda et al., Biochem Pharmacol 2006 : Anti-prostaglandin E2 ( PGE2 ) antiserum or antisense oligonucleotides against PGE2 receptor EP2 or EP3 abrogated inhibitory effects of ketoconazole and terbinafine hydrochloride on TNF-alpha induced NF-kappaB activity and CCL27, CCL2, and CCL5 production, indicating the involvement of endogenous PGE2 in the inhibitory effects ... Carboxyheptyl imidazole also suppressed TNF-alpha induced NF-kappaB activity and CCL27, CCL2, and CCL5 production ... These results suggest that ketoconazole and terbinafine hydrochloride may suppress TNF-alpha induced NF-kappaB activity and CCL27, CCL2, and CCL5 production by increasing PGE2 release from keratinocytes
McFarlane et al., FEBS Lett 2002 : TNF activates pro-inflammatory transcription factor nuclear factor-kappaB (NF-kappaB) by uncertain signalling mechanisms
Zhang et al., Int J Oncol 2002 : Furthermore, NDPP1 is able to inhibit NF-kappaB activation induced by TNF-alpha treatment in HepG2 cells
Said et al., Br J Pharmacol 2002 : 2 : Although unable to induce PGHS-2 expression by itself, TNF-alpha promoted a similar transcription nuclear factor-kappaB (NF-kappaB) activation to IL-1beta
Kim et al., Mol Pharmacol 2008 (Inflammation...) : BOT-64 attenuates NF-kappaB regulated expression of inflammatory genes such as inducible nitric-oxide synthase, cyclooxygenase-2, tumor necrosis factor-alpha , interleukin (IL)-1beta, and IL-6 in LPS activated or expression vector IKKbeta transfected macrophages
Basu et al., Biochem Biophys Res Commun 1998 (Glioma) : The role of DNA-PK, a protein kinase involved in the response to DNA damage, in the activation of NF kappa B by IR and TNF alpha was examined ... In M059J cells, which do not express DNA-PK, IR did not activate NF kappa B, whereas TNF alpha induction of NF kappa B was still observed ... In HeLa cells, wortmannin, an inhibitor of DNA-PK, blocked the induction of NF kappa B by IR but not by TNF alpha ... These results indicate that DNA-PK participates in the activation of NF kappa B by IR but not by TNF alpha
Taylor et al., J Virol 2006 (Cytomegalovirus Infections) : Furthermore, IE86 expression inhibits tumor necrosis factor alpha induced NFkappaB DNA binding and target gene expression
Li et al., Am J Physiol Regul Integr Comp Physiol 2000 : We recently reported that tumor necrosis factor-alpha (TNF-alpha) rapidly activates NF-kappaB in differentiated skeletal muscle myotubes and that TNF-alpha acts directly on the muscle cell to induce protein degradation
Hess et al., Neurosci Lett 1996 : TNF activated nuclear factor kappa B (NF kappa B) in HBMEC
Malhotra et al., Biochem Biophys Res Commun 2002 : There was no difference, however, in the ability of heat shock to inhibit TNFalpha mediated NF-kappaB activation , IkappaBalpha degradation, IkappaB kinase activation, and macrophage chemotactic protein-1 expression in the HSF-1-/- cells compared to the HSF-1+/+ cells
Kilpatrick et al., Am J Physiol Cell Physiol 2002 : Inhibition of either PKC-delta or PI 3-kinase attenuated TNF-alpha mediated activation of the antiapoptotic transcription factor NFkappaB
Tanaka et al., Curr Drug Metab 2010 : We found that either UVB, IL-1 or TNFalpha could induce NF-kappaB by activating its signal transduction pathway
Rahman et al., Mol Cell Biochem 2002 (Inflammation) : Oxidative stress and TNF-alpha induce histone acetylation and NF-kappaB/AP-1 activation in alveolar epithelial cells : potential mechanism in gene transcription in lung inflammation ... H2O2, and TNF-a , and TSA all increased NF-kappaB and AP-1 DNA binding to their consensus sites assessed by the electrophoretic mobility shift assay
Vogel et al., Am J Physiol Lung Cell Mol Physiol 2006 : TNF-alpha caused a progressive increase in NF-kappaB activity after 0.5 h and ICAM-1 protein expression two- to threefold of basal after 2 h. Untreated lungs expressed a low and constant level of ICAM-1 between 0 and 3.5 h. TNF-alpha failed to induce NF-kappaB activation and ICAM-1 expression in lungs of NADPH oxidase-deficient mice lacking p47(phox)
Tan et al., Biochim Biophys Acta 1994 : Our results indicate that both PAF and TNF stimulate the expression of NF-kappa B p50/p105 in vivo
Colell et al., FEBS Lett 2002 (Colonic Neoplasms) : Exogenous neutral ( N ) and acidic ( A ) SMase activated NF-kappa B with different kinetics, accounting for the diverse pattern of DNA binding of NF-kappa B complexes activated by tumor necrosis factor-alpha (TNF)
Lee et al., Biochem Biophys Res Commun 2005 (Fibrosarcoma) : We also found that SM-7368 strongly inhibits TNF-alpha induced NF-kappaB activity but not AP-1 activity
Punzón et al., Int Immunopharmacol 2003 : At the molecular level, EXPLY-37 did not inhibit the activation of the nuclear factor kappa B (NF-kappaB) transcription factor by TNF
Uhl et al., Viral Immunol 2009 (Asthma...) : To investigate the underlying mechanism ( s ) for the increased TNF-alpha expression, the role of nuclear factor-kappaB (NF-kappaB) , an important regulator of TNF-alpha gene transcription, was examined
Im et al., Eur J Immunol 1997 : These data suggest that PAF, which is released immediately or shortly after LPS injection, induces the expression of TNF-alpha through the activation of NF-kappa B
Kang et al., FASEB J 2006 : TNF-alpha increased NF-kappaB expression and its activation, and dexamethasone partially reversed these effects
Sethi et al., J Immunol 2007 : Indeed we found that TNF induced NF-kappaB activation was abrogated in MKK4 gene deleted cells, as determined by DNA binding ... NF-kappaB reporter assay revealed that NF-kappaB activation induced by TNF , TNFR1, TRADD, TRAF2, NIK, and I kappaB alpha kinase was modulated in gene deleted cells
Han et al., Gut 2007 (Colitis) : Growth hormone activated STAT5, and directly reduced TNFalpha activation of NFkappaB , in T84 cells
Langen et al., Am J Physiol Cell Physiol 2002 : NAC decreased TNF-alpha induced activation of NF-kappaB only marginally, indicating that the redox-sensitive component of the inhibition of myogenic differentiation by TNF-alpha occurred independently, or downstream of NF-kappaB
Xiao et al., Biol Reprod 2002 : TNFalpha induced IkappaB phosphorylation and NFkappaB activation
Saegusa et al., J Pathol 2007 (Endometrial Neoplasms) : In cell lines, overexpression of p65 inhibited beta-catenin/TCF4 mediated transcription, while transfection of GSK-3beta resulted in repression of TNF-alpha induced NF-kappaB activity
Laza-Stanca et al., J Virol 2006 (Asthma...) : Rhinovirus replication in human macrophages induces NF-kappaB dependent tumor necrosis factor alpha production ... Finally, we established the requirement for NF-kappaB but not p38 mitogen activated protein kinase in induction of TNF-alpha
Ichiyama et al., Inflamm Res 2004 : We examined whether or not IVIG inhibits TNF-alpha induced activation of transcription factor NF-kappaB , a factor that is essential for the expression of proinflammatory cytokines, in human coronary artery endothelial cells ( CAEC ) ... The inhibitory effect of IVIG on NF-kappaB activation induced by TNF-alpha was evaluated by Western blot analysis and ELISA ... Western blot analysis and ELISA demonstrated that IVIG inhibits NF-kappaB activation induced by TNF-alpha in CAEC ... The data suggest that IVIG inhibits NF-kappaB activation induced by TNF-alpha in CAEC, thereby possibly modulating IL-6 production and E-selectin expression
Yuan et al., Eur J Pharmacol 2009 (Inflammation...) : Ghrelin also significantly suppressed interleukin-1beta, tumor necrosis factor-alpha , and endothelin-l mRNA expression, and inhibited NF-kappaB activation
Shibata et al., Atherosclerosis 2007 : Mulberry leaf aqueous fractions inhibit TNF-alpha induced nuclear factor kappaB (NF-kappaB) activation and lectin-like oxidized LDL receptor-1 ( LOX-1 ) expression in vascular endothelial cells ... Furthermore, mulberry leaf aqueous fractions inhibited TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB) and phosphorylation of inhibitory factor of NF-kappaB-alpha ( IkappaB-alpha ) in a time- and concentration dependent fashion
Trinh et al., Arch Pharm Res 2009 (Pruritus) : These curcuminoids also inhibited degranulation, protein expression of TNF-alpha and IL-4, and transcription factor NF-kappaB activation in IAC induced RBL-2H3 cells
Luo et al., Nephrol Dial Transplant 2008 (Acute Kidney Injury...) : MT downregulated the TNF-alpha mRNA level, and PDTC inhibited the increases in both NF-kappaB translocation and TNF-alpha mRNA
Moon et al., J Cell Physiol 2004 : Furthermore, the transactivation of TNF-alpha stimulated NF-kappaB and AP-1 was inhibited by U0126 treatment ... Overexpression of RasN17 also abolished the TNF-alpha stimulated NF-kappaB and AP-1 activity
DeLuca et al., Virology 1998 : Inhibition of TNFalpha induced NF-kappaB activation using the antioxidant N-acetylcysteine (NAC) resulted in increased apoptosis in both U937 and U9-IIIB cells, while preactivation of NF-kappaB with the non-apoptotic inducer IL-1beta caused a relative decrease in apoptosis ... The protective role of NF-kappaB in blocking TNFalpha- and HIV-1 induced apoptosis was supported by studies in Jurkat T cells engineered to express IkappaB alpha repressor mutants ( TD-IkappaB ) under the control of a tetracycline-responsive promoter
Bonizzi et al., Biochem Pharmacol 1999 : The PKC inhibitor calphostin C inhibited NF-kappaB activation by tumor necrosis factor (TNF)-alpha or interleukin (IL)-1beta in Jurkat or NIH3T3 cells but not in MCF7 A/Z cells
Ohkita et al., Eur J Pharmacol 2003 : On the other hand, a spontaneous NO donor ( +/- ) - ( E ) -4-ethyl-2- [ ( E ) -hydroxyimino ] -5-nitro-3-hexanamide ( FK409 ) significantly attenuated the basal and tumor necrosis factor-alpha induced endothelin-1 production and NF-kappaB activation in endothelial cells
Shishodia et al., Oncogene 2006 : Diosgenin suppressed TNF induced NF-kappaB activation as determined by DNA binding, activation of IkappaBalpha kinase, IkappaBalpha phosphorylation, IkappaBalpha degradation, p65 phosphorylation, and p65 nuclear translocation through inhibition of Akt activation
Balasubramanian et al., J Biol Chem 2011 : The interferon-gamma induced GTPase, mGBP-2, inhibits tumor necrosis factor alpha (TNF-alpha) induction of matrix metalloproteinase-9 (MMP-9) by inhibiting NF-kappaB and Rac protein
Ohkita et al., Jpn J Pharmacol 2002 : Electrophoretic mobility shift assay showed that proteasome inhibitors diminished TNF-alpha stimulated nuclear factor-kappa B (NF-kappaB) activation in ECs
Horvath et al., Exp Hematol 2006 (Leukemia, Promyelocytic, Acute) : The apoptotic potential of M8 and RV was compared using a specific double staining method and inhibition of TNF-alpha induced activation of NF-kappaB was studied
Chen et al., Kidney Int 2008 (Hypertension...) : Candesartan suppressed TNF induced chemokine expression and NFkappaB activation independent of AT1R blockade in cultured renal tubular epithelial cells
Callaghan et al., J Leukoc Biol 1996 (Leukemia, Myelomonocytic, Acute) : Additionally, TNF-alpha induced PTK and NF-kappa B activation were important in collagenase gene expression in this cell line
Ohkawa et al., Biochim Biophys Acta 1999 : Using antisense oligonucleotides, we confirmed that the TNF-alpha-stimulation of HA synthesis by MRC-5 cells is dependent on the activation of the p50/p65 NF-kappa B complex
Ashton et al., J Biol Chem 2003 : Inhibition of tumor necrosis factor alpha mediated NFkappaB activation and leukocyte adhesion, with enhanced endothelial apoptosis, by G protein linked receptor ( TP ) ligands ... IBOP abrogated TNFalpha induced NFkappaB activation in endothelial cells, as determined by reduced IkappaB phosphorylation and NFkappaB nuclear translocation, by inhibiting the assembly of signaling intermediates with the intracellular domain of TNF receptors 1 and 2 in response to TNFalpha
Raschi et al., Blood 2003 (Antiphospholipid Syndrome...) : Delta TRAF6 and Delta MyD88 significantly abrogate antibody induced as well as IL-1- or LPS induced NF-kappa B activation, whereas Delta TRAF2 ( involved in NF-kappa B activation by TNF ) does not affect it
Abbas et al., J Biol Chem 2003 : We have shown previously that tumor necrosis factor alpha-(TNF) strongly induces osteoclastogenesis of preosteoclasts and do so through activation of the transcription factor, NF-kappaB
Köhler et al., Vet Immunol Immunopathol 1999 : Involvement of reactive oxygen species in TNF-alpha mediated activation of the transcription factor NF-kappaB in canine dermal fibroblasts ... With respect to a possible therapeutic modulation of TNF-alpha mediated activation of Nuclear Factor-kappa B (NF-kappaB) in canine cutaneous inflammation, we investigated the role of NF-kappaB and the involvement of reactive oxygen species ( ROS ) in the TNF-alpha signalling pathway in dermal fibroblasts of the dog ... Peroxides, therefore, possibly play an important part in the redox-sensitive pathway of TNF-alpha dependent NF-kappaB activation in canine skin
Deeb et al., Front Biosci (Elite Ed) 2011 : DNA binding assays revealed that combination treatment suppressed both basal and TNF-alpha induced NF-kappaB activation
Hattori et al., Cardiovasc Res 2000 (Diabetic Angiopathies) : High glucose or mannitol also enhanced TNFalpha stimulated NF-kappaB activity ... An antioxidant N-acetyl-L-cysteine and a selective protein kinase C ( PKC ) inhibitor GF109203X significantly suppressed the TNFalpha induced NF-kappaB activation, and abrogated potentiation of TNFalpha induced NF-kappaB activity caused by high glucose ( 27.5 mmol/l )
Clermont et al., Cell Signal 2003 : ( 1 ) Although nuclear factor-kappa B (NF-kappaB) was activated by TNF , its inhibition by MG-132 failed to sensitize these cells ... These results indicate that TNF activates NF-kappaB , which does not drive any anti-apoptotic response, and p38, which plays an anti-apoptotic function probably through HSP27 phosphorylation
Park et al., Front Biosci (Elite Ed) 2011 (Inflammation) : TNF-alpha mediated NF-kappaB activation is constantly extended by transglutaminase 2 ... In the TGase 2-inducible EcR23/TG cell line, TGase 2 over-expression resulted in sustained activation of NF-kappa B in the presence of TNF-alpha , for up to 24 hrs, while in the absence of TGase 2 induction, NF-kappaB activity was restored to basal levels within 6 hrs of TNF-alpha treatment
Huber et al., Placenta 2006 : Induction of the inhibitor may involve TNFalpha stimulated activation of NFkappaB
Li et al., Molecular cancer 2010 : In this study, we demonstrate that MA significantly enhanced TNFalpha induced inhibition of pancreatic cancer cell proliferation, invasion, and potentiated TNFalpha induced cell apoptosis by suppressing TNFalpha induced NF-kappaB activation in a dose- and time dependent manner ... Our data demonstrate that MA can potentiate the anti-tumor activities of TNFalpha and inhibit pancreatic tumor growth and invasion by activating caspase dependent apoptotic pathway and by suppressing NF-kappaB activation and its downstream gene expression
Antras-Ferry et al., FEBS Lett 1997 : Moreover this glucocorticoid did not suppress the TNF-alpha induced increase of NF-kappaB binding activity
Cárcamo et al., Biochemistry 2002 : Vitamin C suppresses TNF alpha induced NF kappa B activation by inhibiting I kappa B alpha phosphorylation ... Tumor necrosis factor-alpha (TNFalpha) activates NFkappaB via a well defined kinase pathways involving NFkappaB inducing kinase (NIK), which activates downstream multisubunit IkappaB kinases (IKK) ... We found that intracellular vitamin C inhibits TNFalpha induced activation of NFkappaB in human cell lines ( HeLa, monocytic U937, myeloid leukemia HL-60, and breast MCF7 ) and primary endothelial cells ( HUVEC ) in a dose dependent manner ... Vitamin C-loaded cells showed significantly decreased TNFalpha induced nuclear translocation of NFkappaB , NFkappaB dependent reporter transcription, and IkappaBalpha phosphorylation
Jutooru et al., J Biol Chem 2010 (Pancreatic Neoplasms) : Results of Sp1, Sp3, and Sp4 knockdown by RNA interference demonstrate that both p50 and p65 are Sp-regulated genes and that inhibition of constitutive or tumor necrosis factor induced NFkappaB by curcumin is dependent on down-regulation of Sp1, Sp3, and Sp4 proteins by this compound
Yoneshima et al., Int J Antimicrob Agents 2003 : We tested the hypothesis that fosfomycin inhibits the activation of NF-kappaB induced by tumor necrosis factor-alpha (TNF-alpha) in human monocytic U-937 cells, and a T cell line ( Jurkat )
Higuchi et al., J Control Release 2005 : Intracellular distribution of NFkappaB decoy and its inhibitory effect on TNFalpha production by LPS stimulated RAW 264.7 cells
Yorek et al., Am J Physiol 1998 : In bovine aorta and bovine pulmonary artery endothelial cells, TNF-alpha activated nuclear factor (NF)-kappa B
True et al., Am J Physiol Lung Cell Mol Physiol 2000 : Challenge with H ( 2 ) O ( 2 ) also failed to induce NF-kappaB-driven reporter gene expression in the transduced HMEC-1 cells, whereas TNF-alpha increased the NF-kappaB-driven gene expression approximately 10-fold
Higuchi et al., Pharmazie 2006 (Drug-Induced Liver Injury) : The production of TNFalpha was significantly inhibited by cationic liposomes/NFkappaB decoy complex but not by cationic liposomes/random decoy complex or naked NFkappaB decoy
Kazmi et al., J Cell Biochem 1995 : TNF-alpha induced NF kappa B was also inhibited by tepoxalin in HeLa cells, while relatively less marked inhibition was observed in Jurkat cells
Wen et al., J Pharmacol Exp Ther 2006 : ML120B concentration-dependently inhibits tumor necrosis factor alpha (TNFalpha) stimulated NF-kappaB signaling via inhibition of IkappaBalpha phosphorylation, degradation, and NF-kappaB translocation into the nucleus
Li , J Pharmacol Sci 2003 (Bacterial Infections...) : Among those signaling pathways, activation of NF-kappaB leads to up-regulation of IL-1beta, IL-8 and TNF-alpha , mucin MUC2 and Toll-like receptor 2 (TLR2), whereas activation of p38 MAP kinase mediates not only up-regulation of inflammatory mediators and mucin MUC5AC but also down-regulation of TLR2
Holden et al., Cell Signal 2008 : The PKC selective inhibitors, Ro31-8220, Gö6976, GF109203X and Gö6983, revealed no effect on TNFalpha induced NF-kappaB DNA binding and a similar lack of effect on serine 32/36 phosphorylated IkappaBalpha and the loss of total IkappaBalpha indicates that activation of the core IKK-IkappaBalpha-NF-kappaB cascade by TNFalpha does not involve PKC
Yang et al., J Neurosci 2002 : Moreover, little nuclear factor-kappaB (NF-kappaB) translocation is induced by TNF-alpha in neurons of TNFRI -/-, whereas NF-kappaB subunit p65 is still translocated from the cytoplasm into the nucleus in neurons from wild-type and TNFRII -/- mice
Beck et al., FEBS Lett 1996 : We found that binding of NFkappaB is strongly induced in mesangial cells by both IL-1beta and TNF-alpha
Declercq et al., J Immunol 1998 : Remarkably, TRAF2-DN overexpression, which was found to inhibit the TNF dependent recruitment of endogenous wild-type TRAF2 to the TNF-R75 signaling complex, could neither block TNF-R55- or TNF-R75 induced NF-kappaB activation nor granulocyte/macrophage-CSF secretion ... Possibly, additional factors different from TRAF2 are involved in TNF mediated NF-kappaB activation
Kang et al., Biochem Pharmacol 2005 : These results suggest that the inhibitory effect of equol on TNF-alpha expression is mediated , at least in part, by blocking NF-kappaB activation and the inhibition of TNF-alpha expression by equol might be involved in its osteoprotective effect
Yang et al., J Immunol 2004 : The zinc finger mutation C417R of I-kappa B kinase gamma impairs lipopolysaccharide- and TNF mediated NF-kappa B activation through inhibiting phosphorylation of the I-kappa B kinase beta activation loop ... Also, LPS- and TNF induced NF-kappaB transcription was inhibited by IKKgammaC417R ... Collectively, our results indicated that the zinc finger structure of IKKgamma plays a key role in LPS- and TNF induced NF-kappaB activation
Kai et al., FEBS Lett 2009 : We recently reported that diacylglycerol kinase (DGK) alpha enhanced tumor necrosis factor-alpha (TNF-alpha) induced activation of nuclear factor-kappaB (NF-kappaB) ... These results strongly suggest that DGKalpha positively regulates TNF-alpha dependent NF-kappaB activation via the PKCzeta mediated Ser311 phosphorylation of p65/RelA
Pandey et al., Endocrinology 2002 : In contrast, cell stimulation with the cytokine TNFalpha allowed activation of NFkappaB through phosphorylation, ubiquitination, and subsequent degradation of IkappaBalpha
Rackley et al., J Urol 1999 : In normal human urothelial cells, activation of the NF-kappaB complex in response to stimulation with TNF-alpha , LPS, and dsRNA was detected by immunohistochemical methods and EMSA
Sandur et al., Carcinogenesis 2007 : The results indicate that the relative potency for suppression of tumor necrosis factor (TNF) induced nuclear factor-kappaB (NF-kappaB) activation was Cur > DMC > BDMC ; thus suggesting the critical role of methoxy groups on the phenyl ring ... Turmerones also failed to inhibit TNF induced NF-kappaB activation
Lee et al., J Neuroimmunol 1998 : We determined that binding sites for both NF-kappaB ( -186 bp region ) and C/EBP ( -198 bp region ) are involved in TNF-alpha and IL-1beta mediated ICAM-1 upregulation
Roebuck et al., J Biol Chem 1995 : The results indicate that H2O2 activates ICAM-1 transcription through AP-1/Ets elements within the ICAM-1 promoter, which are distinct from NF-kappa B-mediated ICAM-1 expression induced by TNF alpha
Haas et al., Mol Cell 2009 : LUBAC enhances NEMO interaction with the TNF-RSC, stabilizes this protein complex, and is required for efficient TNF induced activation of NF-kappaB and JNK, resulting in apoptosis inhibition
Charbonneau et al., J Biol Chem 2007 (Anoxia...) : By the use of HIF-1-deficient cells and by obliterating NF-kappaB activation, it was determined that the hypoxic TACE response is mediated by HIF-1 signaling, whereas the regulation by TNFalpha also requires NF-kappaB activation
Zhang et al., Immunity 2000 : The adapter protein RIP plays a crucial role in NF-kappaB activation by TNF
Bohuslav et al., J Clin Invest 1998 : In the present study, which uses mice with genetic deletions of the proteins of NF-kappaB complex, we provide data demonstrating that increased expression of the p50 subunit of NF-kappaB directly results in the downregulation of LPS induced TNF production
Wu et al., Gynecol Obstet Invest 2010 (Endometriosis) : TNFalpha stimulation activated NF-kappaB through induction of IkappaB phosphorylation, but the activation can be suppressed by TSA
Mayo et al., J Biol Chem 2002 : PTEN blocks tumor necrosis factor induced NF-kappa B-dependent transcription by inhibiting the transactivation potential of the p65 subunit ... In this report, we utilized PTEN as a natural biological inhibitor of Akt activity to study the effects on tumor necrosis factor (TNF) induced activation of NF-kappaB ... We found that the reintroduction of PTEN into prostate cells inhibited TNF stimulated NF-kappaB transcriptional activity ... PTEN failed to block TNF induced IKK activation , IkappaBalpha degradation, p105 processing, p65 ( RelA ) nuclear translocation, and DNA binding of NF-kappaB
Paludan et al., Arch Virol 2000 : Herpes simplex virus type 2 infection of macrophages impairs IL-4 mediated inhibition of NO production through TNF-alpha induced activation of NF-kappaB ... Furthermore, neutralisation of residual TNF-alpha activity or inhibition of NF-kappaB activation largely restored the inhibitory effect of IL-4
Sun et al., Acta Anaesthesiol Scand 2004 : Endotoxin caused transient production of TNF-alpha and IL-6 and activation of NF-kappaB in the intestine at peak times of 1, 4 and 1 h, respectively
Li et al., Cytokine 2008 : Positive and negative signaling components involved in TNFalpha induced NF-kappaB activation ... Stimulation of cells with TNFalpha triggers activation of NF-kappaB through various signaling molecules, including TRAF2, RIP, MAP3K, and the IKK complex
Zwacka et al., J Gene Med 2000 (Colorectal Neoplasms) : In order to provide further understanding of determinants of TNF-alpha responses, we studied TNF-alpha induced NF-kappaB activation and variable tumour responses
Webb et al., J Dent Res 2002 (Osteosarcoma) : Transfection of NFkappaB and C/EBPbeta-sensitive reporter promoter constructs demonstrated that NFkappaB activity was enhanced and that constitutive C/EBPbeta was inhibited by TNF-alpha , with both effects being p38 MAPK dependent
Loncar et al., Gut 2003 (Colitis) : and aims : Tumour necrosis factor alpha (TNF-alpha) induction of nuclear factor kappaB (NFkappaB) activation plays a major role in the pathogenesis of inflammatory bowel disease ( IBD )
Kaileh et al., J Biol Chem 2007 (Inflammation) : In this study we show that the leave extract of WS, as well as its major constituent withaferin A ( WA ), potently inhibits NFkappaB activation by preventing the tumor necrosis factor induced activation of IkappaB kinase beta via a thioalkylation-sensitive redox mechanism, whereas other WS-derived steroidal lactones, such as withanolide A and 12-deoxywithastramonolide, are far less effective
Matsumoto et al., Clin Cancer Res 2005 (Breast Neoplasms) : In this cell line, DHMEQ completely inhibited the tumor necrosis factor-alpha induced activation of NF-kappaB
Jiang et al., Respir Med 2005 : We therefore evaluated the effects of pyrrolidine dithiocarbamate ( PDTC ; 0.1 mM ) and spermine NONOate ( Sper-NO ; 1 mM ) on adhesion molecule expression and nuclear factor kappa B (NF-kappaB) activation induced by TNF-alpha ( 10 ng/ml ) in cultured human pulmonary microvascular endothelial cells ( PMVEC ) ... The mRNA expression of E-selectin, ICAM-1 and VCAM-1, and activation of NF-kappaB induced by TNF-alpha for 2 h were decreased significantly by the above two pretreatments
Ji et al., Anticancer Drugs 2009 (Lung Neoplasms) : TNF-alpha treatment induced an elevated activated NFkappaB/p65 , concomitant with induced p21 levels, which resulted in increased sensitivity to gefitinib in PC-9-ZD cells
Sekine et al., J Cell Physiol 2000 : Synergistic activation of NF-kappab and inducible isoform of nitric oxide synthase induction by interferon-gamma and tumor necrosis factor-alpha in INS-1 cells ... TNF-alpha did not affect STAT1 activation, but stimulated DNA binding and transcriptional activity of NF-kappaB , both of which were further increased by IFN-gamma ... In conclusion, IFN-gamma activates STAT1 and potentiates TNF-alpha induced NF-kappaB activation in INS-1 cells, thereby inducing iNOS and cell destruction
Cook et al., Proc Natl Acad Sci U S A 2002 : E1A expression did not block upstream events in TNF-alpha induced activation of NF-kappa B in NIH 3T3 cells, including degradation of I kappa B-alpha, nuclear translocation of NF-kappa B subunits, and their dimeric binding to kappa B sequences in the nucleus
Yoon et al., J Agric Food Chem 2007 (Breast Neoplasms) : The present study examines the effects of selected phytochemicals and apple extracts on TNF-alpha induced NF-kappaB activation in human breast cancer MCF-7 cells ... Apple extracts significantly inhibited the TNF-alpha induced NF-kappaB activation at a dose of 5 mg/mL ( p < 0.05 ) ... Curcumin also significantly blocked the TNF-alpha induced NF-kappaB activation at doses of 10 and 20 microM ( p < 0.05 ) ... These results suggest that apple extracts and curcumin have the capabilities of inhibiting TNF-alpha induced NF-kappaB activation of MCF-7 cells by inhibiting the proteasomal activities instead of IkappaB kinase (IKK) activation
Haycock et al., J Biol Chem 2000 (Melanoma) : We have previously shown that alpha-melanocyte stimulating hormone ( alpha-MSH ) can oppose tumor necrosis factor alpha activation of NF-kappaB ( 1-2 h ) and intercellular adhesion molecule 1 up-regulation ( mRNA by 3 h and protein by 24 h ) in melanocytes and melanoma cells
Thommesen et al., J Immunol 1998 : We show that TNF induced activation of NF-kappaB was inhibited by the well-known selective inhibitors of cytosolic phospholipase A2 (cPLA2) : the trifluoromethyl ketone analogue of arachidonic acid ( AACOCF3 ) and methyl arachidonyl fluorophosphate ... The trifluoromethyl ketone analogue of eicosapentaenoic acid ( EPACOCF3 ) also suppressed TNF induced NF-kappaB activation and inhibited in vitro cPLA2 enzyme activity with a similar potency as AACOCF3 ... The arachidonyl methyl ketone analogue ( AACOCH3 ) and the eicosapentanoyl analogue ( EPACHOHCF3 ), which both failed to inhibit cPLA2 enzyme activity in vitro, had no effect on TNF induced NF-kappaB activation ... TNF induced NF-kappaB activation was also strongly reduced in cells stimulated in the presence of the secretory PLA2 ( sPLA2 ) inhibitors 12-epi-scalaradial and LY311727
Benito et al., Ann Rheum Dis 2005 (Disease Progression...) : The inflammatory cell infiltrate, blood vessel formation, and angiogenic factors, NF-kappaB activation , expression of tumour necrosis factor alpha (TNFalpha) and interleukin 1beta (IL1beta), and the presence of cyclo-oxygenase (COX)-1 and COX-2 were quantified
Seno et al., Scand J Gastroenterol 2002 : TNF-alpha inhibition reduced the NF-kappaB activation, and blocked a transient wave of epithelial cell proliferation 12 h after the injection
Milligan et al., Anticancer Res 2001 (Adenocarcinoma...) : TNF induced I kappa B proteolysis and NF-kappa B nuclear translocation ; however, this was blocked by pretreatment with proteasome inhibitors
Hozumi et al., Am J Physiol Lung Cell Mol Physiol 2001 (Pneumonia) : Increased expression of MMP-9 and NF-kappa B activation induced by TNF-alpha were inhibited by pyrrolidine dithiocarbamate and N-acetyl-L-cysteine but were not inhibited by curcumin
Chen et al., Mol Cell 2002 : Geldanamycin ( GA ), an antitumor agent that disrupts the formation of this heterocomplex, prevents TNF induced activation of IKK and NF-kappaB
Suominen et al., Eur J Endocrinol 2004 : Rat seminiferous tubule segments were cultured in the presence and absence of TNF-alpha , infliximab and SN50, a NF-kappa B inhibitor
Aupperle et al., J Immunol 1999 (Arthritis, Rheumatoid) : Transfection with a dominant negative IKK-2 mutant prevented TNF-alpha mediated NF-kappa B nuclear translocation, whereas a dominant negative IKK-1 mutant had no effect
Wang et al., Virus Res 2007 : Further experiments showed that IL-6 and TNF-alpha production were dependent on NF-kappaB , which was activated through I-kappaBalpha degradation
Kim et al., J Immunol 2008 (Bacterial Infections...) : Consistently, Nod2-null macrophages tolerant to LPS and MDP showed enhanced production of TNF-alpha and IL-6 as well as increased NF-kappaB and MAPK activation in response to the dipeptide KF1B, the Nod1 agonist
Delfino et al., Mol Endocrinol 1998 : Tumor necrosis factor-alpha , which is produced by round spermatids in the testis, increased nuclear NF-kappaB binding activity when added to Sertoli cells
Friedman et al., J Virol 2002 : Inhibition of tumor necrosis factor alpha induced NF-kappa B activation by the adenovirus E3-10.4/14.5K complex ... We demonstrate here that the E3 region of Ad inhibits the activation of NF-kappa B induced by tumor necrosis factor alpha (TNF-alpha) and interleukin-1
Peng et al., Cell Mol Life Sci 2000 : In the present study, we investigated the effect of pycnogenol, an antioxidant phytochemical, on the activation of NF-kappa B and the induction of VCAM-1 and ICAM-1 in tumor necrosis factor (TNF)-alpha treated human umbilical vein endothelial cells ( HUVECs ) ... Gel-shift analysis of HUVEC demonstrated that pretreatment with pycnogenol exhibited a concentration dependent suppression of TNF-alpha induced activation of NF-kappa B
Coletti et al., EMBO J 2002 (Cachexia) : It has been proposed that TNFalpha mediated activation of NFkappaB leads to down regulation of MyoD, however the mechanisms underlying TNFalpha effects on skeletal muscle remain poorly understood
Zhou et al., Biochim Biophys Acta 2006 : A gel shift assay further showed that TNF-alpha induced Nuclear Factor-kappaB (NF-kappaB) activity was significantly reduced by cryptotanshinone
Manna et al., J Immunol 2000 : Vesnarinone suppresses TNF induced activation of NF-kappa B , c-Jun kinase, and apoptosis ... Vesnarinone blocked TNF induced activation of NF-kappa B in a concentration- and time dependent manner ... The effects of vesnarinone were not cell type specific, as it blocked TNF induced NF-kappa B activation in a variety of cells ... Vesnarinone also blocked NF-kappa B activation induced by several other inflammatory agents, inhibited the TNF induced activation of transcription factor AP-1, and suppressed the TNF induced activation of c-Jun N-terminal kinase and mitogen activated protein kinase kinase
Lin et al., Mol Cell Biol 2003 : Although TRAF2 is known to be required for TNF induced JNK and NF-kappaB activation , the underlying mechanism of the increased sensitivity of TRAF2 null cells ( TRAF2 ( -/- ) ) to TNF induced apoptosis is not fully understood
Han et al., J Clin Invest 2001 : TNF-alpha mediates SDF-1 alpha induced NF-kappa B activation and cytotoxic effects in primary astrocytes ... Late Erk1/2 activation was involved in TNF-alpha stimulated NF-kappa B activation and cytokine induction
Klatt et al., Biologicals 2007 : Furthermore, we evaluated PKR, IL-6, and TNF-alpha induction , as well as IkappaB degradation and NFkappaB activation as control parameters of non-specific siRNA effects
Cheng et al., Genes Dev 1996 : TANK, a co-inducer with TRAF2 of TNF- and CD 40L mediated NF-kappaB activation
Legler et al., Immunity 2003 : Recruitment of TNF receptor 1 to lipid rafts is essential for TNFalpha mediated NF-kappaB activation
Otsuka et al., Cancer Res 1999 (Glioma) : Activation of NF-kappaB by TNF-alpha in the resistant cell lines resulted in a significant increase of a reporter gene expression driven by NF-kappaB site, suggesting a possibility that activation of p50-p65 confers resistance to TNF-alpha
Kamiyama et al., Biosci Biotechnol Biochem 2008 : cDNA microarray analysis revealed that EPQB decreased TNF-alpha induced expression of NF-kappaB target genes
Enk et al., J Ethnopharmacol 2007 (Translocation, Genetic) : Differential effect of Rhizoma coptidis and its main alkaloid compound berberine on TNF-alpha induced NFkappaB translocation in human keratinocytes
Pincheira et al., J Immunol 2008 : TNFR1/c-Src, but not TNFR1/Jak2, plays an obligate role in the activation of NF-kappaB by TNF , whereas TNFR1/Jak2, but not TNFR1/c-Src, plays an obligate role in the activation of STAT3
Li et al., J Biol Chem 2004 : BRE, brain and reproductive organ expressed protein, was found previously to bind the intracellular juxtamembrane domain of a ubiquitous death receptor, tumor necrosis factor receptor 1 ( TNF-R1 ), and to down-regulate TNF-alpha induced activation of NF-kappaB
Baker et al., Glia 2010 : This expression also partially requires the intermediate production of TNF-alpha and subsequent NF-kappaB activation via TNF-R1
Burow et al., Biochem Biophys Res Commun 2000 : PI3K and AKT cell survival signaling were correlated with increased TNF stimulated NF-kappaB activity in MCF-7 cells
Feuillard et al., Cytokine 1991 (Lymphoma) : In the present work, we investigated the molecular events leading to NF-kappa B activation by TNF alpha in a human T cell line ( Jurkat ) and its subclone JCT6, which presents a deficiency in the PKA transduction pathway ... Thus, TNF alpha induced NF-kappa B activation was found to be mediated by none of the major signal mediating kinases such as protein kinase C ( PKC ), protein kinase A, or Ca ( 2+ ) -regulated kinases ... Furthermore, we found that cytoplasmic acidification facilitated NF-kappa B activation by both TNF alpha and PKC, by a mechanism that increases NF-kappa B/I kappa B dissociation without affecting the NF-kappa B translocation step
Yi et al., Int Immunol 2001 : CpG DNA and LPS synergistically induce TNF-alpha production in RAW264.7 cells and J774 cells through activation of NF-kappaB ... Furthermore, transient transfection with a super-repressive mutant of IkappaBalpha ( IkappaBalpha-AA ) demonstrated that NF-kappaB plays a critical role in CpG DNA mediated TNF-alpha expression
Higai et al., Biol Pharm Bull 2006 (Adenocarcinoma...) : TNFalpha stimulation induced the biphasic increases in expression of NFkappaB-p65 , ST3Gal I, FUT IV, ST3Gal IV and ST6GalNAc III mRNAs and the transient increase in expression of ST6Gal I mRNA and the decrease in ST3GalNAc IV mRNA
Zhu et al., Dermatol Surg 2007 (Keloid) : Abnormal nuclear factor (NF)-kappaB signal pathway and aspirin inhibits tumor necrosis factor alpha induced NF-kappaB activation in keloid fibroblasts ... To examine the effect of aspirin on the tumor necrosis factor (TNF)-alpha induced NF-kappaB activation in keloid fibroblasts ... In this study, we demonstrate that TNF-alpha induced NF-kappaB activation in keloid fibroblasts, which show more sensitively than the normal skin fibroblasts ... Aspirin pretreatment can inhibit TNF-alpha induced activation of NF-kappaB in a dose dependent manner by preventing the phosphorylation and degradation of IkappaBalpha and nuclear translocation of NF-kappaB
Kurokouchi et al., J Bone Miner Res 2000 : In the present study, we investigated effects of a synthetic glucocorticoid, dexamethasone ( Dex ), on TNF-alpha dependent activation of NF-kappaB and expression of the ICAM-1 gene ... Electrophoretic mobility shift assay ( EMSA ) revealed that TNF-alpha dependent activation of NF-kappaB was almost completely suppressed by Dex treatment
Ng et al., J Biol Chem 1994 : Although tumor necrosis factor (TNF) and interleukin-1 ( IL ) induce the expression of the IL-8, TNF stimulated gene 6, and plasminogen activation inhibitor-2 genes and the activation of NF-kappa B with nearly identical kinetics, the two cytokines differ significantly in the induction of the IL-6 gene in all primary fibroblasts tested
Xiao et al., Immunol Lett 2007 : Taken together, these results suggest that linomide inhibits TNF-alpha production by suppressing the activation of NF-kappaB and mitogen activated protein kinase ( MAPK ), which might, at least in part, contribute to the beneficial effects of linomide in the treatment of autoimmune diseases
Rascle et al., Exp Cell Res 2009 : Inhibition of NF-kappaB activity by short interfering RNA mediated knock-down of p65 impairs, while activation of NF-kappaB activity by TNF-alpha synergizes induction of NF-kappaB target genes by LMX1B
Fu et al., J Vet Med Sci 2009 (Inflammation) : Vaspin did not inhibit the TNF-alpha ( 20 min ) activation of JNK, p38 and NF-kappaB , but only slightly inhibited Akt
Yin et al., Yao Xue Xue Bao 2005 : To investigate the regulatory effects of various anti-inflammatory drugs on both endogenous and TNFalpha induced NF-kappaB activation as well as the relative biological activity ... Dexamethasone ( 1 x 10 ( -8 ) mol.L ( -1 ) ) and meloxicam ( 1 x 10 ( -7 ) - 1 x 10 ( -6 ) mol.L ( -1 ) ) can decrease both endogenous and TNFalpha induced NF-kappaB activation ... However, its influence on TNFalpha induced NF-kappaB activation is needed for further study
Kim et al., BMB Rep 2008 : Furthermore, increased DNA binding activity of NF-kappaB in response to TNF-alpha , an agonist of NF-kappaB signaling, was seen in differentiated but not undifferentiated mouse ES cells
Cho et al., J Cell Physiol 2010 (Calcinosis) : In this study, we determined the effect of TNF-alpha on osteogenic differentiation of stromal cells derived from human adipose tissue ( hADSC ) and the role of NF-kappaB activation on TNF-alpha activity ... TNF-alpha activated ERK and increased NF-kappaB promoter activity
Cinar et al., Biochem J 2004 (Prostatic Neoplasms) : We observed that activation of NF-kappaB by TNFalpha ( tumour necrosis factor alpha ) inhibited both basal and androgen stimulated PSA expression, and that this down-regulation occurred at the promoter level, as confirmed by the super-repressor IkappaBalpha ( S32A/S36A ), a dominant negative inhibitor of NF-kappaB
Wullaert et al., J Biol Chem 2007 : Similar to the other ABINs, ABIN-3 binds to A20 and inhibits NF-kappaB activation induced by tumor necrosis factor , interleukin-1, and 12-O-tetradecanoylphorbol-13-acetate
Wang et al., Mol Cell Biol 2000 : As a consequence, TNF-alpha mediated I-kappaB degradation and NF-kappaB activation were markedly enhanced in Stat1-deficient cells, whereas overexpression of Stat1 in 293T cells blocked NF-kappaB activation by TNF-alpha
Hall et al., Am J Physiol Heart Circ Physiol 2005 : Taken together, these data provide compelling evidence for the role of NF-kappaB signaling in TNF-alpha gene expression in heart and highlight the importance of this proinflammatory gene-regulatory pathway as a potential therapeutic target in the management of cytokine induced myocardial dysfunction
Shimada et al., J Pharmacol Exp Ther 1999 : NF-kappaB activation in response to TNF-alpha , IL-1beta, phorbol ester, and H ( 2 ) O ( 2 ) was down-regulated by polaprezinc
Geisler et al., Gastroenterology 2007 (Drug-Induced Liver Injury) : RelA/p65 is essential for TNF induced NF-kappaB activation in adult hepatocytes
Zernecke et al., FASEB J 2003 : Only at high concentrations, cPG enhanced TNF-alpha induced cell death and inhibited TNF-alpha induced IkappaB-alpha kinase (IKK) activation, IkappaB-alpha degradation, and NF-kappaB/p65 translocation, while promoting AP-1/c-jun phosphorylation
Ronaldson et al., Mol Pharmacol 2010 (MAP Kinase Signaling System) : Pharmacologic inhibition of nuclear factor-kappaB (NF-kappaB) signaling with SN50 prevented both TNF-alpha release and Mrp1 expression changes in astrocytes triggered with gp120 ; however, SN50 did not attenuate Mrp1 expression in cells triggered with TNF-alpha
Herbein et al., Curr HIV Res 2008 : Since tumor necrosis factor alpha (TNFalpha) activates NF-kappaB and AP-1, we investigated the role of exogenous Nef protein in TNFalpha stimulated U937 cells and primary macrophages ... We observed that exogenous Nef and TNFalpha synergistically activate NF-kappaB and AP-1 in U937 cells and primary macrophages resulting in enhanced stimulation of the HIV-1 long terminal repeat ( LTR ), and subsequently in enhanced viral replication in both chronically infected promonocytic U1 cells and acutely HIV-1 infected primary macrophages
Zhan et al., J Immunol 2008 : Glucocorticoid induced TNF receptor expression by T cells is reciprocally regulated by NF-kappaB and NFAT
Israël et al., EMBO J 1989 : This strongly suggests that induction of an NF kappa B-like activity is responsible for TNF-alpha stimulation of mouse MHC class I genes
Shen et al., Am J Physiol Gastrointest Liver Physiol 2009 (Liver Diseases, Alcoholic) : Taken together, our findings suggest that SIRT1-NF-kappaB signaling is involved in regulating LPS- and metabolites-of-ethanol mediated TNF-alpha production in rat Kupffer cells and in murine macrophages
Ulanova et al., Biochem Biophys Res Commun 2006 : Inhibition of Syk down-regulated TNF induced p38 and p44/42 MAPK phosphorylation and nuclear translocation of p65 NF-kappaB
Warzocha et al., Eur Cytokine Netw 1995 (Second Messenger Systems) : Since induction of AP-1, IRF-1 and IRF-2 as well as NF-GMa proceeds through translational event, the posttranslational TNF/LT-driven activation of NF-kappa B remains the only cellular event identified so far that serves as a direct target in their signaling cascade
Hayashi et al., J Biol Chem 2000 : Both DNA binding assays and luciferase assays revealed that TNF-alpha induced NF-kappaB activation is defective in T2 cells
Chan et al., J Biol Chem 1994 (Lymphoma, Large B-Cell, Diffuse) : These results, overall, suggest that although TNF receptors are essential for induction of NF-kappa B , a small percentage is sufficient to fully transduce this signal
Amrani et al., J Immunol 1999 : Using EMSA, we show that stimulation with TNF-alpha or IL-1 beta induces NF-kappa B DNA binding activity in human airway smooth muscle cells ... In cells transfected with the luciferase reporter, dexamethasone did not affect TNF-alpha induced NF-kappa B-dependent transcription
Yamasaki et al., Clin Exp Immunol 2002 (Arthritis, Rheumatoid) : Electrophoretic mobility shift assay ( EMSA ) showed a significant reduction in the DNA binding activity of NF-kappaB in troglitazone treated FLS in response to TNF-alpha or IL-1beta
Zhang et al., FASEB J 2001 : Alpha-lipoic acid inhibits TNF-alpha induced NF-kappaB activation and adhesion molecule expression in human aortic endothelial cells ... Furthermore, LA dose-dependently inhibited TNF-alpha induced IkappaB kinase activation, subsequent degradation of IkappaB, the cytoplasmic NF-kappaB inhibitor, and nuclear translocation of NF-kappaB ... In contrast, TNF-alpha induced NF-kappaB activation and adhesion molecule expression were not affected by ascorbic acid or by manipulating cellular glutathione status with l-2-oxo-4-thiazolidinecarboxylic acid, N-acetyl-l-cysteine, or d, l-buthionine-S, R-sulfoximine
Giri et al., J Biol Chem 1998 (Lymphoma, T-Cell...) : TNF activated NF-kappaB in Jurkat cells but not in HuT-78 cells
Sanlioglu et al., J Biol Chem 2001 : The present study demonstrates for the first time that the activation of Rac1 ( a GTP binding protein ), and the subsequent production of ROS, constitutes a major pathway involved in NFkappaB mediated tumor necrosis factor-alpha (TNFalpha) secretion following LPS challenge in macrophages ... IKKalpha and IKKbeta were both required downstream modulators of LPS activated Rac1, since the expression of either of the IKK dominant mutants ( IKKalphaKM or IKKbetaKA ) drastically reduced NFkappaB dependent TNFalpha secretion
Cao et al., Chin Med J (Engl) 2004 : TNF-alpha induced NF-kappaB activation was measured by electrophoretic mobility shift assay ( EMSA ) ... The results of EMSA showed that NF-kappaB activation by TNF-alpha in HL-60 cells was induced in a dose dependent manner, but was almost completely inhibited by mutant IkappaBalpha repressor in transfected cells
Murali et al., Proc Natl Acad Sci U S A 2005 (Arthritis, Experimental) : TNF-alpha induced NF-kappaB and p38 kinase activities and clinical symptoms of collagen induced arthritis in mice were all diminished
Goldfeld et al., J Exp Med 1991 : Therefore, TNF-alpha mRNA induction by PMA, like its induction by virus and LPS, is not primarily mediated by NF-kappa B , but rather is mediated through other sequences and protein factors
Thoh et al., J Biol Chem 2010 : Azadirachtin blocks TNF induced activation of nuclear factor kappaB (NF-kappaB) and also expression of NF-kappaB dependent genes such as adhesion molecules and cyclooxygenase 2
Mormina et al., Eur J Pharmacol 2006 : TNF-alpha induced release of IL-8 was inhibited by WIN55212-2 which also blocked the degradation of IkappaB-alpha and activation of NF-kappaB induced by TNF-alpha
Boonkaewwan et al., J Agric Food Chem 2008 : It was previously demonstrated that stevioside attenuates NF-kappaB dependent TNF-alpha and IL-1beta synthesis in LPS stimulated monocytes
Lin et al., J Biol Chem 2006 : Here, we show that the receptor interacting protein ( RIP ), a key mediator of tumor necrosis factor induced NF-kappaB and JNK activation, plays a key role in IGF-I receptor signaling
Lee et al., Exp Mol Med 2010 : TNF-alpha activated the NF-kappaB and the JNK pathways
Folmer et al., Biochem Pharmacol 2006 : In order to understand underlying regulatory mechanisms, inhibition of both NF-kappaB-driven reporter gene expression and TNFalpha induced binding of NF-kappaB to a consensus response element was achieved at concentrations of 320 microM ( flavokavain A ), 175 microM ( flavokavain B ) and 870 microM ( kavain and dihydrokavain )
Meyer-Bahlburg et al., Clin Exp Rheumatol 2004 : TNF-alpha expression can be mediated by transcription factor nuclear factor-kappa B (NF-kappaB) ... In vitro expression of TNF-alpha and activation of NF-kappaB in synovial fibroblasts after infection with Yersinia enterocolitica or Salmonella enteritidis was analysed by electrophoretic mobility shift assay, Western blot assay and real-time PCR
Wright et al., J Cell Physiol 1999 : IL-1beta and TNF-alpha stimulated nuclear NF-kappaB levels by about fourfold and fivefold, respectively, in HASMCs
Gray-Schopfer et al., Cancer Res 2007 (MAP Kinase Signaling System...) : The survival mechanism requires nuclear factor-kappaB (NF-kappaB) transcription factor activity, which is strongly induced by TNF-alpha in these cells
Zhu et al., J Virol 1998 : Furthermore, we observed that the core protein blocked the TNF induced activation of RelA/NF-kappaB in murine BC10ME cells, thus at least partially accounting for the increased sensitivity of BC10ME cells to TNF
Kang et al., Biol Pharm Bull 2007 : Cornuside treatment attenuated tumor necrosis factor-alpha (TNF-alpha) induced nuclear factor-kappa B (NF-kappaB) p65 translocation in HUVECs
Ge et al., Vascul Pharmacol 2007 : Since EMMPRIN and MMP-9 up-regulation is associated with activation of the NF-kappaB pathway, we investigated the effect of pioglitazone and resveratrol on TNF-alpha induced NF-kappaB activation
Bodine et al., Endocrinology 1999 (Bone Resorption) : Treatment of the cells with 17beta-E2 partially suppressed the activation of NF-kappaB by TNF alpha , but did not block cytokine induced IL-6 secretion
Ide et al., J Nutr 2001 : Effects of SAC on hydrogen peroxide ( H ( 2 ) O ( 2 ) ) or tumor necrosis factor (TNF)-alpha induced nuclear factor (NF)-kappa B activation were determined ... SAC also inhibited H ( 2 ) O ( 2 ) - or TNF-alpha induced NF-kappa B activation
Qi et al., J Cell Physiol 2009 : Of note, TNF-alpha induced NF-kappaB may be the primary pathway contributing to CXCL10 production in THP-1 cells
Leghmari et al., Cell Immunol 2008 : Using chemical inhibitors we show that ( i ) both ERK1/2 MAP kinase and NF-kappaB transcription factor play an important role in IL-10 and TNF-alpha production, in macrophages stimulated by Tat
Wahl et al., J Clin Invest 1998 (Colonic Diseases) : TNFalpha induced nuclear translocation of NF-kappaB was prevented by sulfasalazine through inhibition of IkappaBalpha degradation
Shifera et al., Immunol Lett 2010 : Also, 1alpha,25 ( OH ) ( 2 ) D ( 3 ) did not suppress the activation of NF-kappaB by TNFalpha or PHA
Chung et al., J Biol Chem 2002 : Because the CTAR2 region interacts with the tumor necrosis factor ( TNF-alpha receptor associated death domain protein, it is interesting to find that BRAM1 also interferes with NF-kappaB activation mediated by TNF-alpha ... BRAM1 interferes LMP1 mediated and TNF-alpha induced NF-kappaB activation by targeting IkappaBalpha molecules
Yin et al., Br J Haematol 2000 (Leukemia) : Forskolin inactivated NF-kappaB in K/Dau600 cells but not in K562 cl. 6 cells, whereas TNF activated NF-kappaB in K562 cl.6 cells but not in K/Dau600 cells
Nam et al., J Nat Prod 2010 : Fijiolides A and B, inhibitors of TNF-alpha induced NFkappaB activation, from a marine derived sediment bacterium of the genus Nocardiopsis ... Fijiolide A reduced TNF-alpha induced NFkappaB activation by 70.3 %, with an IC ( 50 ) value of 0.57 micro-M
Pellegatta et al., Am J Clin Nutr 2003 : The nuclear appearance of p50-NFkappaB and p65-NFkappaB acutely induced by TNF-alpha was not modified by resveratrol but was increased after overnight incubation with resveratrol alone or in combination with TNF-alpha
Rao et al., J Interferon Cytokine Res 1995 : Upregulation of NF-kappa B-dependent gene expression mediated by the p75 tumor necrosis factor receptor
Kubota et al., J Mol Cell Cardiol 2001 (Heart Failure) : Furthermore, TNF- alpha overexpression activated NF- kappa B , a mediator of anti-apoptotic pathways, in the myocardium
Higuchi et al., Blood 1995 (Leukemia, Myeloid) : TPCK also blocked TNF induced activation of nuclear factor (NF)-kappa B
Chang et al., Nephron. Experimental nephrology 2006 (Inflammation) : NF-kappaB was activated within 30 min by tumor necrosis factor-alpha (TNF-alpha) or interleukin-1beta (IL-1beta) ... Induction of NF-kappaB within 30 min by TNF-alpha- and IL-1beta was mediated through intracellular calcium but not ROS ... Silymarin inhibited TNF-alpha induced calcium dependent NF-kappaB activation irrespective of its antioxidant effect
Wang et al., Biochemistry 2008 : In a kinase gene screen using a NF-kappaB reporter, we observed that overexpression of casein kinase 1alpha (CK1alpha) enhanced TNFalpha induced NF-kappaB activation, and a CK1alpha kinase dead mutant, CK1alpha ( K46A ), reduced NF-kappaB activation induced by TNFalpha
Dezube et al., J Cardiovasc Pharmacol 1995 (HIV Infections) : TNF also induces expression of human immunodeficiency virus ( HIV ) through activation of the transcription factor NF-kappa B , which binds to the viral long terminal repeat ( LTR )
Landowski et al., Oncogene 2003 (Multiple Myeloma) : This DNA binding activity consisted primarily of RelB-p50 heterodimers, which was distinct from the NF-kappa B activation of TNF alpha
Lee et al., J Biol Chem 2000 : Incubation of macrophages with PAO1 led to NF-kappaB dependent expression of inducible nitric-oxide synthase, COX-2, and tumor necrosis factor-alpha , which was unaffected by inhibition of Rac1 or Cdc42 function
Zhang et al., Br J Pharmacol 2000 : Nuclear NF-kappaB activity was robustly induced by TNF-alpha
Baudy et al., Int Immunopharmacol 2009 (Myositis) : Since NF-kappaB is an important therapeutic target, we decided to utilize an in vitro screening assay to identify potential inhibitors that block TNF-alpha induced NF-kappaB activation in a C2C12 muscle line stably expressing an NF-kappaB luciferase reporter gene
Bratt et al., Clin Exp Immunol 2000 : To examine the effects of AF and GSTM on TNF-alpha induced NF-kappaB activation this was measured in HUVEC nuclear extracts by an electrophoretic mobility shift assay ... AF, but not GSTM, decreased TNF-alpha induced NF-kappaB activation in HUVEC
Nicolas et al., Dev Biol 2005 (Muscular Atrophy) : We generated transgenic mice carrying a C-terminal deleted form of PW1 ( DeltaPW1 ) which blocks p53 mediated cell death and TNFalpha mediated NFkappaB activation fused to the myogenin promoter
Wilson et al., J Virol 2006 : Furthermore, both ectopically expressed SV5 SH and MuV SH blocked activation of NF-kappaB by TNF-alpha in a reporter gene assay, suggesting that both SH proteins can inhibit TNF-alpha signaling
Wadgaonkar et al., J Cell Biochem 2005 : Endothelial cell myosin light chain kinase (MLCK) regulates TNFalpha induced NFkappaB activity ... Both TNFalpha induced increase in NFkappaB dependent transactivation measured by NFkappaB luciferase reporter assay ( approximately fivefold ) and nuclear translocation of NFkappaB were significantly inhibited by MLCK-selective inhibitors, KT5926 ( 60 % inhibition of luciferase activity ) and ML7 ( 50 % decrease ) ... Furthermore, our data revealed that inhibition of MLCK attenuated the TNFalpha induced IkappaB phosphorylation, translocation of p65, NFkappaB-DNA binding, and NFkappaB transcriptional activity ... Molecular approaches to either reduce EC MLCK expression ( AdV EC MLCK antisense construct ) or to reduce kinase activity ( kinase-dead EC MLCK ATPdel mutant ) produced similar attenuation of the TNFalpha induced NFkappaB response ... Together, these novel observations indicate that TNFalpha induced cytoskeletal rearrangement driven by MLCK activity is necessary for TNFalpha dependent NFkappaB activation and amplification of pro-survival signals
Kim et al., Mol Cell Biol 2005 : Here we report that eIF4GI, which is a scaffold protein interacting with many translation factors, interacts with TRAF2, a signaling molecule that plays a key role in activation of NF-kappaB through TNF-alpha
Aosasa et al., Shock 2001 : The present study shows that the inhibitory effect of GM on the TNFalpha production of activated human monocytes is mediated by the suppression of NFkappaB activation, while the mechanism of UTI inhibiting TNFalpha production of human monocytes may be due to the inhibition of either the translation or secretion of TNFalpha
Chae et al., Immunopharmacol Immunotoxicol 2004 : TNF-gamma ( 10 ng/ml ) increased NF-kappaB DNA binding activity in nuclear extracts of osteoblasts ... The addition of NAC ( N-acetyl cysteine ), free radical scavenger, completely prevented TNF-alpha induced activation of NF-kappaB
Hohmann et al., J Biol Chem 1990 : TNF-alpha and TNF-beta activate the transcription factor NF-kappa B in HL60 and HEp2 cells ... Maximal activation of NF-kappa B required binding of TNF-alpha or TNF-beta to 20-25 % of the total number of TNF receptors and was achieved within minutes after the addition of TNF-alpha to HL60 cells ... Both TNF-alpha and TNF-beta activate NF-kappa B at 5-10-fold lower concentrations in HL60 cells compared with HEp2 cells, and this correlates well with their different affinities for binding to these cells
Ogino et al., Biochim Biophys Acta 2005 : We have previously reported that monochloramine ( NH ( 2 ) Cl ), a neutrophil derived oxidant, inhibited tumor necrosis factor alpha (TNFalpha) induced expression of cell adhesion molecules and nuclear factor-kappaB (NF-kappaB) activation ( Free Radical Research 36 ( 2002 ) 845-852 ) ... Here, we studied the mechanism how NH ( 2 ) Cl inhibited TNFalpha induced NF-kappaB activation, and compared the effects with taurine chloramine ( Tau-NHCl ) ... Although Tau-NHCl alone had little effects on TNFalpha induced NF-kappaB activation, simultaneous presence of Tau-NHCl and ammonium ion significantly inhibited the NF-kappaB activation, probably through the conversion of Tau-NHCl to NH ( 2 ) Cl ... These results indicated that NH ( 2 ) Cl inhibited TNFalpha induced NF-kappaB activation through the oxidation of IkappaB, and that NH ( 2 ) Cl is physiologically more relevant than Tau-NHCl in modifying NF-kappaB mediated cellular responses
Lewis et al., J Biol Chem 2000 : The death domain kinase, receptor interacting protein ( RIP ), is one of the major components of the tumor necrosis factor receptor 1 (TNFR1) complex and plays an essential role in tumor necrosis factor (TNF) mediated nuclear factor kappaB (NF-kappaB) activation ... These data indicate that Hsp90 plays an important role in TNF mediated NF-kappaB activation by modulating the stability and solubility of RIP
Adcock et al., Life Sci 1994 : The interaction of the transcription factors, activator protein 1 (AP-1) and nuclear factor kappa from B cells ( NF kappa B ) with DNA and glucocorticoid receptors was analysed by gel mobility shift assays following stimulation by tumour necrosis factor alpha (TNF alpha) and a phorbol ester ( PMA ) that activates protein kinase C. PMA and TNF alpha both caused significant ( 180-340 % ) increases in AP-1 and NF kappa B DNA binding which peaked at 15 minutes and decreased to a constant elevated level at between 1-3 hrs and was sustained for 24hrs
Takada et al., J Biol Chem 2006 (Neoplasm Invasiveness) : We found that in several human cancer cell lines, SAHA potentiated the apoptosis induced by tumor necrosis factor (TNF) and chemotherapeutic agents and inhibited TNF induced invasion and receptor activator of NF-kappaB ligand induced osteoclastogenesis, all of which are known to require NF-kappaB activation ... Because several of these genes are regulated by NF-kappaB, we postulated that SAHA mediates its effects by modulating NF-kappaB and found that SAHA suppressed NF-kappaB activation induced by TNF , IL-1beta, okadaic acid, doxorubicin, lipopolysaccharide, H ( 2 ) O ( 2 ), phorbol myristate acetate, and cigarette smoke ; the suppression was not cell type-specific because both inducible and constitutive NF-kappaB activation was inhibited
Tomsig et al., Biochem J 2004 : The introduction of calcium into HEK293 cells either through the activation of muscarinic cholinergic receptors or through the application of the ionophore A23187 was found to enhance TNF-alpha dependent activation of NF-kappaB
de Rossi et al., Anesth Analg 2004 : LPS induced production of TNF-alpha and IL-6 as well as activation of NF-kappaB were significantly increased in the presence of xenon compared with controls
Sappey et al., AIDS Res Hum Retroviruses 1995 : DFO also protected against PMA induced NF-kappa B activation as well as TNF-alpha induced HIV-1 activation
Majumdar et al., Oncogene 2003 (Leukemia, Myeloid) : The treatment of these cells with adenosine suppressed TNF induced NF-kappaB activation, but had no effect on activation of another redox-sensitive transcription factor, AP-1 ... The effect on TNF induced NF-kappaB activation was selective as adenosine had minimal effect on NF-kappaB activation induced by H ( 2 ) O ( 2 ), PMA, LPS, okadaic acid, or ceramide, suggesting differences in the pathway leading to NF-kappaB activation by different agents ... The suppression of TNF induced NF-kappaB activation by adenosine was found not to be because of inhibition of TNF induced IkappaBalpha phosphorylation and degradation or IkappaBalpha kinase activation ... The suppression of TNF induced NF-kappaB activation was unique to adenosine, as neither its metabolites ( inosine, AMP, and ATP ) nor pyrimidines ( thymidine and uridine ) had any effect ... Overall, our results clearly demonstrate that adenosine selectively suppresses TNF induced NF-kappaB activation, which may contribute to its role in suppression of inflammation and of the immune system
Ahn et al., Cancer Res 2007 (Skin Neoplasms) : In agreement with this, we also found that TNF activated NQO2, and NQO2-specific small interfering RNA abrogated the TNF induced NQO2 activity and NF-kappaB activation
Srinivasula et al., J Biol Chem 1999 : Expression of the CARD domain of human CLAP abrogates tumor necrosis factor-alpha induced NF-kappaB activation, suggesting that cellular CLAP plays an essential role in this pathway of NF-kappaB activation
Scheinman et al., Science 1995 : Stimulation by tumor necrosis factor causes the release of NF-kappa B from I kappa B alpha
Ozes et al., Nature 1999 : Here we show that the Akt serine-threonine kinase is involved in the activation of NF-kappaB by tumour necrosis factor (TNF) ... Wortmannin ( a PI(3)K inhibitor ), dominant negative PI(3)K or kinase-dead Akt inhibits TNF mediated NF-kappaB activation ... Thus, both Akt and NIK are necessary for TNF activation of NF-kappaB ... Mutation of this amino acid blocks phosphorylation by Akt or TNF and activation of NF-kappaB
Li et al., J Bone Miner Res 2007 (Osteoporosis) : The effect of TNFalpha induced NF-kappaB signaling on Smad signaling and on in vitro osteoblast mineralization was examined using specific NF-kappaB inhibitors and activators, and effects of TNFalpha induced NF-kappaB signaling on BMP-2 induced Runx2 mRNA were examined using RT-PCR
Howard et al., Proc Natl Acad Sci U S A 1993 : Transcriptional activation of the HIV long terminal repeat and subsequent increase in virus production are linked to TNF activation of the cellular transcription factor NF-kappa B
Granja et al., J Immunol 2006 : Furthermore, we present evidence that A238L inhibits the activation of TNF-alpha by modulating NF-kappaB , NF-AT, and c-Jun trans activation through a mechanism that involves CREB binding protein/p300 function, because overexpression of these transcriptional coactivators recovers TNF-alpha promoter activity
Bai et al., J Cell Biochem 2009 (Liver Neoplasms...) : Based on our previous observations that the nutrient flavonoid luteolin potently blocks TNF induced NF-kappaB activation in cancer cells, we investigated if the combination of SMC3 and luteolin would achieve a synergistic anticancer activity
Routes et al., J Virol 2005 : Like E1A, E6 has been reported to sensitize cells to lysis by TNF-alpha by inhibiting the TNF-alpha induced activation of NF-kappaB ... We found that E1A, but not E6, blocked the TNF-alpha induced activation of NF-kappaB , an activity that correlated with E1A-p300 binding
Sethi et al., J Biol Chem 2006 : As examined by the DNA binding of NF-kappaB, we found that indirubin suppressed tumor necrosis factor (TNF) induced NF-kappaB activation in a dose- and time dependent manner
Rahman et al., Am J Physiol Cell Physiol 2000 : We observed that PKC-zeta also induced the TNF-alpha induced NF-kappaB binding to the ICAM-1 promoter and the resultant ICAM-1 gene transcription
Fairchild et al., Am J Physiol Cell Physiol 2005 : While HT is generally thought to have anti-inflammatory and cytoprotective effects, we have previously shown that moderate in vitro HT prolongs TNF-alpha production by LPS stimulated mononuclear phagocytes, in part by prolonging TNF-alpha gene transcription and activation of the pleiotropic transcription factor NF-kappaB
Rivas et al., Breast Cancer Res Treat 2010 (Breast Neoplasms...) : Moreover, TNFalpha promoted ErbB-2/ErbB-3 heterocomplex formation, Akt activation and NF-kappaB transcriptional activation ... Inhibition of ErbB-2 by addition of AG825, an epidermal growth factor receptor/ErbB-2-tyrosine kinase inhibitor, or knockdown of ErbB-2 by RNA interference strategy, blocked TNFalpha induced NF-kappaB activation and proliferation
Widera et al., BMC neuroscience 2006 : Furthermore, TNF strongly activated nuclear factor-kappa B (NF-kappaB) as measured by reporter gene assays and by an activity-specific antibody ... TNF mediated activation of IKK-beta resulted in activation of NF-kappaB and was followed by up-regulation of the bona-fide target gene cyclin D1
Linderson et al., Int J Colorectal Dis 2005 (Crohn Disease...) : We sought to determine whether CARD15 mediated NF-kappaB activation can contribute to MDP induced TNFalpha production and, consequently, if polymorphisms in both genes affect the control of such induction ... Transfection and electrophoretic mobility shift assays ( EMSA ) experiments in HEK293 cells demonstrated that MDP exposure stimulates TNFalpha gene transcription, as a result of CARD15 induced NF-kappaB activation and binding to TNFalpha promoter
Basile et al., J Biol Chem 1997 : Role of NF-kappaB in tumor necrosis factor-alpha and interleukin-1beta regulation
Yan et al., Mol Cell Biochem 2008 (Prostatic Neoplasms) : YXQG-EQ treatment inhibited constitutive and epidermal growth factor induced Akt phosphorylation, basal and TNF-alpha induced NF-kappaB activation, and downregulated anti-apoptotic Bcl-2 and Bcl-xL expression
Grech et al., J Biol Chem 2005 : Tumor necrosis factor (TNF) superfamily receptors typically induce both NF-kappaB and JNK activation by recruiting the TRAF2 signal transduction protein to their cytoplasmic domain
Delhalle et al., Oncogene 2002 (Adenocarcinoma) : Therefore, MnSOD expression is induced by NF-kappaB in epithelial cancer cells in response to TNF-alpha , and is at least partially responsible for their resistance to TNF-alpha induced apoptosis, presumably through the clearance of death inducing ROS
Dai et al., J Cell Mol Med 2010 : We report here that HSP70 over-expression in human colon cancer cells can inhibit TNFalpha induced NFkappaB activation but promote TNFalpha induced activation of c-Jun N-terminal kinase (JNK) through interaction with TNF receptor (TNFR) associated factor 2 ( TRAF2 ) ... Therefore, our study suggests that HSP70 may differentially regulate TNFalpha induced activation of NFkappaB and JNK through interaction with TRAF2, contributing to the pro-apoptotic roles of HSP70 in TNFalpha induced apoptosis of human colon cancer cells
Zeng et al., J Neurochem 2005 (Infection) : 3-OMS also inhibited nuclear factor kappaB (NF-kappaB) binding activity induced by TNF-alpha or by the combination of TNF-alpha and Abeta
Riedemann et al., Immunity 2003 (Sepsis) : We now report that in vitro exposure of neutrophils to C5a causes increased levels of IkappaBalpha, decreased NF-kappaB dependent gene transcription of TNFalpha , and decreased lipopolysaccharide (LPS) induced TNFalpha production
Jones et al., J Immunol 2005 (Pneumonia, Pneumococcal) : Lung NF-kappaB activation and neutrophil recruitment require IL-1 and TNF receptor signaling during pneumococcal pneumonia
Kuno et al., Int Immunol 1994 (Niemann-Pick Diseases) : Furthermore, activation of NFkB was also induced in NPD type A fibroblasts in response to IL-1 alpha and TNF-alpha stimulation to a similar extent as in normal fibroblasts
Yoshizumi et al., Exp Cell Res 2004 : Finally, TNF-alpha induced activator protein-1 (AP-1) and nuclear factor-kappaB (NF-kappaB) activation and resultant intracellular adhesion molecule-1 ( ICAM-1 ) and vascular cell adhesion molecule-1 ( VCAM-1 ) expressions were inhibited by ebselen
Ting et al., EMBO J 1996 : We have created mutant cell lines that are unable to activate NF-kappaB in response to TNF
Feng et al., Mol Cell Biol 2005 : A prominent function of p62 is the regulation of NF-kappaB activation in response to interleukin-1 (IL-1) and tumor necrosis factor signaling through the formation of an aPKC/p62/TRAF6 multiprotein signaling complex
Andoh et al., Clin Exp Immunol 1999 : The EMSAs indicated that sodium butyrate suppressed TNF-alpha induced nuclear factor (NF)-kappaB- and activation protein (AP)-1-DNA binding activity, but enhanced TNF-alpha induced activation of CCAAT/enhancer binding protein ( C/EBP)beta (NF-IL-6 ) -DNA binding activity
Poppema , Hematology / the Education Program of the American Society of Hematology. American Society of Hematology. Education Program 2005 (Hodgkin Disease) : A key role is played by the presence of constitutive nuclear factor (NF)-kappaB , which is induced by LMP1, as well as by CD30, CD40, tumor necrosis factor (TNF)-alpha, and Notch1 interactions, and results in the upregulation of at least 45 genes including chemokines, cytokines, receptors, apoptotic regulators, intracellular signaling molecules, and transcription factors
Mikenberg et al., Eur J Cell Biol 2006 : Tumor necrosis factor alpha (TNF-alpha) activates the degradation of IkappaB-alpha and the nuclear import of cytoplasmic NF-kappaB
Roulston et al., J Exp Med 1992 (Acquired Immunodeficiency Syndrome) : NF-kappa B binding activity was inducible by tumor necrosis factor and phorbol myristate acetate in PLB-985
Ohba et al., J Orthop Res 2009 (Intervertebral Disc Displacement) : TNF-alpha induced NF-kappaB signaling reverses age related declines in VEGF induction and angiogenic activity in intervertebral disc tissues
Schindler et al., Cell Signal 2010 : In secondary assays, we demonstrated that hexokinase 1 does not alter TNF dependent activation of NF-kappaB or JNK modules
Bianchi et al., Neurobiol Aging 2010 (Encephalitis...) : We show here that : ( 1 ) S100B also stimulates AP-1 transcriptional activity in microglia via RAGE dependent activation of JNK ; ( 2 ) S100B upregulates IL-1beta and TNF-alpha expression in microglia via RAGE engagement ; and ( 3 ) S100B/RAGE induced upregulation of COX-2, IL-1beta and TNF-alpha expression requires the concurrent activation of NF-kappaB and AP-1
Fukuda et al., Oncol Rep 2008 (Carcinoma, Adenoid Cystic...) : The expression of CYLD and NF-kappaB mRNAs in HSG cells was increased by TNF-alpha
Schotte et al., J Biol Chem 2001 (Neoplasms) : TNF induced activation of NF-kappa B-dependent gene expression is not modulated by LiCl treatment
Mayne et al., Neuroimmunomodulation 1998 (AIDS Dementia Complex...) : TNF-alpha induction was NF-kappaB dependent and was eliminated by inhibiting protein kinase A, phospholipase C and protein tyrosine kinase activity
Kim et al., Oncogene 2000 : In this study, we demonstrate that the proteolytic cleavage of receptor interacting protein ( RIP ) by caspase-8 during TNF induced apoptosis abrogates the stimulatory role of RIP on TNF induced NF-kappaB activation ... Ectopic expression of the pro-apoptotic C-terminal fragment of RIP inhibited TNF induced NF-kappaB activation by suppressing the activity of I-kappaB kinasebeta (IKKbeta) which phosphorylates I-kB, an inhibitor of NF-kappaB, and triggers its ubiquitin mediated degradation
Hall et al., Circ Res 2001 : Deletion of the redox-sensitive domain of Ref-1 significantly inhibited TNF induced NF-kappaB activation ... Moreover, it seems that Ref-1 may act as a critical cofactor, mediating the TNF induced NF-kappaB response in the vascular endothelium
Wu et al., Int Immunopharmacol 2007 : These findings suggest that the inhibition of LPS induced NO formation and TNF-alpha production in microglia by ginsenosides is due to its inhibition of NF-kappaB , which may be the mechanistic basis for the anti-inflammatory effects of ginsenosides
Ghadimi et al., Inflamm Bowel Dis 2010 : In response to apically applied natural commensal-origin DNA, polarized HT-29 and T84 cells enhanced expression of TLR9 in a specific manner, which was subsequently associated with attenuation of TNF-alpha induced NF-kappaB activation and NF-kappaB mediated IL-8 expression
Sugiyama et al., J Biol Chem 1999 : IkappaBalphaM was resistant to stimulus dependent degradation and suppressed NF-kappaB activation induced by TNF-alpha ( 10 ng/ml ) or IL-1beta ( 10 ng/ml )
Makata et al., Naunyn Schmiedebergs Arch Pharmacol 2006 (Mucocutaneous Lymph Node Syndrome) : High-dose IVIG inhibited tumor necrosis factor-alpha (TNF-alpha) induced activation of nuclear factor-kappaB (NF-kappaB) to a greater degree than dexamethasone in human monocytic U937 cells and human coronary arterial endothelial cells ( HCAEC ), but not in human T lymphocytic Jurkat cells
Hattori et al., Diabetologia 2010 : We therefore examined the effect of liraglutide on TNFalpha induced NF-kappaB activation and NF-kappaB dependent expression of proinflammatory genes
Chu et al., Proc Natl Acad Sci U S A 1997 : In contrast to wild-type c-IAP2, a mutant lacking the C-terminal RING domain inhibits NF-kappaB induction by TNF and enhances TNF killing
Seidel et al., Am J Physiol Lung Cell Mol Physiol 2009 (Chronic Disease...) : DMF inhibited TNFalpha induced NF-kappaB p65 phosphorylation, NF-kappaB nuclear entry, and NF-kappaB-DNA complex formation, whereas PDGF-BB appeared not to activate NF-kappaB within 60 min
Wang et al., Cytokine 2009 (Acute Lung Injury) : ZD 7155 also reduced the mRNA expression of TNF-alpha and IL-1 beta, inhibited the activation of NF-kappaB and AP-1, and improved lung histopathology
Diessenbacher et al., J Invest Dermatol 2008 : Although tumor necrosis factor (TNF) related apoptosis inducing ligand ( TRAIL ) and TNF both activate NF-kappaB in human keratinocytes, only TRAIL potently induces apoptosis ... However, when induction of NF-kappaB was inhibited with a kinase dead IKK2 mutant ( IKK2-KD ), TNF- but not TRAIL induced apoptosis was dramatically enhanced
Jabbar et al., Br J Haematol 1994 (Leukemia, Hairy Cell...) : The induction of NF kappa B by TNF is mediated via a novel signalling pathway involving the generation of reactive oxidative intermediates ... Here we have used an electrophoretic mobility shift assay to show that TNF induced NF kappa B in malignant cells isolated from 3/3 HCL and 15/15 B-CLL patients
Kang et al., J Biol Chem 1998 : Moreover, the activation of nuclear factor kappaB (NFkappaB) induced by extracellularly added H2O2 or tumor necrosis factor-alpha was blocked by overproduction of Prx II
Vlahopoulos et al., Blood 1999 : TNFalpha treatment induces a rapid translocation of the 65 kD transcriptional activator NF-kappaB subunit, Rel A, whose binding in the nucleus occurs before changes in intracellular ROS
Morales et al., J Biol Chem 1997 : Despite activation of nuclear factor-kappaB (NF-kappaB) by TNF , this transcription factor was not required for TNF induced transcription of gamma-GCS-HS as revealed by deletion constructs of the gamma-GCS-HS promoter subcloned in a chloramphenicol acetyltransferase reporter vector and transfected into HepG2 cells
Pessara et al., Mol Cell Biol 1990 : Comparison of the TNF-alpha induced factor and lipopolysaccharide induced NF-kappa B in gel mobility shift assays upon partial protease digestion suggests similar DNA binding protein cores
Das et al., Mol Cell Biochem 1995 (Adenocarcinoma...) : Thiol modulation of TNF alpha and IL-1 induced MnSOD gene expression and activation of NF-kappa B ... TNF alpha and IL-1 each can activate NF-kappa B and induce gene expression of manganese superoxide dismutase ( MnSOD ), a mitochondrial matrix enzyme which can provide critical protection against hyperoxic lung injury
Lee et al., J Cell Physiol 2006 : TNF-alpha stimulated translocation of NF-kappaB into the nucleus and degradation of IkappaB-alpha was blocked by helenalin, but not by U0126, SB202190, or SP600125
Lu et al., Am J Physiol Cell Physiol 2002 : Inhibition of NF-kappaB by a specific NF-kappaB inhibitor, caffeic acid phenylethyl ester, or by dominant expression of the NF-kappaB inhibitory subunit IkappaB caused an increase in FasL induced apoptosis and a reduction in TNF-alpha expression
Lawson et al., Hepatology 1998 : Administration of uridine ( 1,000 mg/kg ) did not reduce plasma levels of TNF-alpha and KC, NF-kappaB activation , or polymorphonuclear leukocyte sequestration, but attenuated apoptosis by 90 % to 94 %
Kanda et al., J Allergy Clin Immunol 2005 : IL-17 suppressed TNF-alpha induced CCL27 secretion and mRNA expression and NF-kappaB activity in keratinocytes
Wright et al., J Cell Biochem 1992 : Furthermore, TNF could still activate NFkB in this variant, suggesting that this pathway is not involved in TNF mediated cytotoxicity
Kefer et al., Shock 2001 : We addressed the role of the redox state of endothelial cells in modulating TNFalpha induced NF-kappaB activity ... The effects of TNFalpha on DNA binding activity of NF-kappaB and expression of mRNA encoding ICAM-1 ( an NF-kappaB activated gene ) were studied in human pulmonary artery endothelial ( HPAE ) cells under basal conditions and after decreasing the intracellular glutathione ( GSH ) concentration ... Stimulation of control cells with TNFalpha resulted in reactive oxygen species ( ROS ) generation and activation of NF-kappaB binding to the ICAM-1 promoter and ICAM-1 transcription
Ravi et al., Mol Cell Biochem 2008 (Melanoma) : We also show that caffeine specifically inhibits UV-mediated NF-kappaB activation, but not TNFalpha mediated NF-kappaB activation
Javelaud et al., Oncogene 2000 (Sarcoma, Ewing) : Induction of p21Waf1/Cip1 by TNFalpha requires NF-kappaB activity and antagonizes apoptosis in Ewing tumor cells ... Our results therefore identify p21Waf1/Cip1 as a mediator of the antiapoptotic effect of TNFalpha induced NF-kappaB in Ewing tumor cells
Byun et al., J Bone Miner Res 2005 (Bone Resorption) : Our data show that TNF-alpha increases intracellular ROS in hBMSC and that TNF-alpha and H2O2 induce apoptosis in hBMSC through the activation of JNK and NF-kappaB
Kang et al., Eur J Pharmacol 2005 : PGG treatment attenuated tumor necrosis factor-alpha (TNF-alpha) induced nuclear factor-kappaB (NF-kappaB) p65 translocation in human umbilical vein endothelial cells
Shattuck et al., Mol Cell Biol 1994 (Melanoma) : In Hs294T cells, gel shift analyses indicate that IL-1 and TNF alpha induce NF-kappa B complex formation ; however, transactivation does not occur, suggesting that subtle differences in the NF-kappa B complexes may result in the inability of the cytokines IL-1 and TNF alpha to activate transcription of the MGSA/GRO genes
Blais et al., J Neuropathol Exp Neurol 2001 : Inhibitory action of nitric oxide on circulating tumor necrosis factor induced NF-kappaB activity and COX-2 transcription in the endothelium of the brain capillaries ... This study investigated the hypothesis that nitric oxide ( NO ) acts as an endogenous modulator of TNF induced NF-kappaB signaling and COX-2 transcription in the endothelium of the cerebral capillaries ... These results indicate that eNOS derived NO acts as an endogenous inhibitor of TNF-alpha induced NF-kappaB activity and COX-2 transcription in the endothelium of the cerebral capillaries
Lee et al., Biochem Pharmacol 2003 : Furthermore, treatment of cells with these compounds prevented the TNF-alpha induced expression of anti-apoptotic NF-kappaB target genes Bfl-1/A1, a prosurvival Bcl-2 homologue, and resulted in sensitizing HT-1080 cells to TNF-alpha induced cell death
Szołtysek et al., Acta biochimica Polonica 2008 : TNFalpha induced activation of NFkappaB protects against UV-induced apoptosis specifically in p53-proficient cells
Lee et al., Biochem Pharmacol 2005 (Colonic Neoplasms) : Parallel with inhibitory effect on cell growth, HCA dose dependency inhibited TNF-alpha induced activation of NF-kappaB accompanied with inhibition of the translocation of p50
Yagyu et al., J Clin Endocrinol Metab 2005 (Endometriosis) : The current study showed for the first time that Thal treatment attenuated the expression of IL-8 by reducing TNF-alpha induced NF-kappaB activation ... Here, we show 1 ) that treatment of endometriotic stromal cells with TNF-alpha increased the expression of phosphorylated IkappaBalpha and degradation of total IkappaBalpha, which in turn activates NF-kappaB ; 2 ) Thal significantly inhibits the TNF-alpha induced expression of phosphorylated IkappaBalpha and degradation of IkappaBalpha ; 3 ) TNF-alpha activation induced increased nuclear translocation of NF-kappaB , which was inhibited by pretreatment with either Thal or N-tosyl-L-phenylalanine chloromethyl ketone, an NF-kappaB inhibitor
Lee et al., Biochem Pharmacol 2007 (Colitis...) : TMMC inhibited TNF-alpha induced nuclear factor kappaB (NF-kappaB) activation directly and indirectly
Chen et al., J Pharmacol Exp Ther 2003 : Ad.N17Rac1 did not inhibit TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB) binding activity or inhibitor of NF-kappaB-alpha degradation
Witcher et al., Blood 2003 (Acute Disease...) : Underlying this response was an increase in TNF induced nuclear factor-kappaB (NF-kappaB) DNA binding within 2 hours in the presence of ATRA and activation of NF-kappaB DNA binding and transcriptional activity in response to ATRA alone within 48 hours of ATRA treatment
Harper et al., J Biol Chem 2003 : Taken together, these results show that PKC activation specifically inhibits the recruitment of key obligatory death domain containing adaptor proteins to their respective membrane associated signaling complexes, thereby modulating TRAIL induced apoptosis and TNF induced NF-kappaB activation, respectively
Besnard-Guerin et al., J Biol Chem 2004 : Beta-catenin was translocated into the nucleus, whereas the tumor necrosis factor induced nuclear translocation of NFkappaB was impaired
Wati et al., J Virol 2007 (Dengue) : Additionally, normal cellular TNF-alpha signaling, measured by quantitation of TNF-alpha induced stimulation of transcription from an NF-kappaB-responsive reporter plasmid or NF-kappaB protein nuclear translocation, was blocked in DV-infected MDM and Huh7 cells
Furuno et al., Neurosci Lett 2006 : Neurotrophic factors increase tumor necrosis factor-alpha induced nuclear translocation of NF-kappaB in rat PC12 cells ... Using green fluorescent protein tagged NF-kappaB1 ( GFP-NF-kappaB1 ), we examined here the effects of TNF-alpha and neurotrophic factors on the nuclear translocation of NF-kappaB in PC12 cells ... TNF-alpha induced gradually the translocation of GFP-NF-kappaB1 from the cytoplasm to the nucleus within 60 min. Pretreatment of lactacystin which is a proteasome-specific inhibitor suppressed significantly the nuclear translocation of GFP-NF-kappaB1 after TNF-alpha stimulation ... These results suggested that there is a close correlation between the signaling pathways via TNF receptors and neurotrophin receptors for the NF-kappaB activation, and that NGF and BDNF enhance TNF-alpha induced nuclear translocation of NF-kappaB
Lee et al., J Biol Chem 1999 (Neoplasms) : PG490 potently inhibited TNF-alpha induced activation of NF-kappaB ... Our identification of a compound that blocks TNF-alpha induced activation of NF-kappaB may enhance the cytotoxicity of TNF-alpha on tumors in vivo and limit its proinflammatory effects
Eder et al., Biochem Biophys Res Commun 2009 : The most ancient part, lipid A is crucial in evoking immediate TNF release and activation of NF-kappaB
Guo et al., PloS one 2012 (Inflammation) : BME also attenuated TNFalpha induced activation of NFkappaB in differentiating preadipocytes and partially restored TNFalpha mediated suppression on adipogenesis ... Using a non-adipogenic HEK293 cell line transfected with luciferase reporter genes, we demonstrated that BME reduced basal and TNFalpha induced NFkappaB activity and increased basal and ciglitazone induced PPARgamma activity ; both may contribute to the pro-adipogenic effect of BME in differentiating F442A preadipocytes
Takada et al., Oncogene 2007 : We found that in wild-type mouse embryonic fibroblast (MEF), TNF induced NF-kappaB activation as measured by DNA binding but deletion of PKR abolished this activation
Takeda et al., Science 1999 (Limb Deformities, Congenital...) : In contrast, there was no impairment of NF-kappaB activation induced by either interleukin-1 or tumor necrosis factor-alpha in IKKalpha-deficient embryonic fibroblasts and thymocytes, indicating that IKKalpha is not essential for cytokine induced activation of NF-kappaB
Li et al., Science 1999 : Mouse embryonic fibroblast cells that were isolated from IKK2-/- embryos showed a marked reduction in tumor necrosis factor-alpha (TNF-alpha)- and interleukin-1alpha induced NF-kappaB activity and an enhanced apoptosis in response to TNF-alpha
Madonna et al., Biochim Biophys Acta 2008 : Insulin markedly increased TNF-alpha induced NF-kappaB activation and induced phosphorylated IkappaB-alpha accumulation
Ruiz et al., J Nutr 2007 (Ileitis) : Quercetin inhibits TNF induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells
Zetoune et al., Cytokine 2001 : A20, a TNF inducible gene, inhibits TNF mediated apoptosis as well as NF-kappa B induced by this cytokine
Arimoto et al., Proc Natl Acad Sci U S A 2010 : Virus induced IRF3 and NF-kappaB activation, as well as K27 linked NEMO polyubiquitination, were abrogated in TRIM23 knockdown cells, whereas TRIM23 knockdown had no effect on TNFalpha mediated NF-kappaB activation
Ginis et al., J Cereb Blood Flow Metab 2002 (Brain Ischemia) : TNF-alpha activates a transcription factor NF-kappaB that binds promoters of multiple genes, thus ensuring pleiotropic effects of TNF-alpha
Reddy et al., J Immunol 2000 : Phosphatidylinositol 3-kinase as a mediator of TNF induced NF-kappa B activation ... The activation of transcription factor NF-kappa B by TNF involves the stimulation of a novel signaling cascade ... In this paper we show that phosphatidylinositol 3-kinase ( PI 3-kinase ) may play a pivotal role in TNF mediated activation of NF-kappa B-dependent genes ... A dominant negative mutant of the p85 regulatory subunit of PI 3-kinase, which is a potent inhibitor of PI 3-kinase signaling, effectively blocked the TNF induced expression of an NF-kappa B-dependent reporter gene
Yin et al., J Biol Chem 2001 : Using confocal microscopy and cell fractionation studies, butyrate pretreatment of a human colon cell line ( HT-29 cells ) inhibited the tumor necrosis factor-alpha (TNF-alpha) induced nuclear translocation of the proinflammatory transcription factor NF-kappaB
Yan et al., Mol Cancer Ther 2008 (Neoplasms) : 17-AJB effectively inhibited tumor necrosis factor-alpha induced NF-kappaB activation and induced apoptosis of tumor cells
Sung et al., Mol Pharmacol 2007 : We found that among nine different flavones tested, fisetin was potent in suppressing tumor necrosis factor (TNF) induced NF-kappaB activation
Mori et al., Life Sci 2004 : In ECV304 cells overexpressing ERalpha, E ( 2 ) significantly inhibited the effects of TNF-alpha on NF-kappaB activation, VCAM-1 expression, and U937 cell adhesion
Blázovics et al., Orv Hetil 2004 (Inflammatory Bowel Diseases) : It became known, that oxidized LDL can inhibit LPS induced binding of the NF-kappaB to DNA and the subsequent expression of TNF-alpha and interleukin-1beta in macrophages as well as oxidized LDL modulates activation of NF-kappaB in mononuclear phagocytes by altering the degradation of I-kappaBs
Tomizawa et al., Diab Vasc Dis Res 2008 : HMW adiponectin requires a shorter incubation period to demonstrate inhibition against tumour necrosis factor alpha (TNFalpha) induced NF-kappaB activation, compared with gAd
Udalova et al., Infect Immun 2000 : Direct evidence for involvement of NF-kappaB in transcriptional activation of tumor necrosis factor by a spirochetal lipoprotein ... By overexpressing disabled mutant IkappaBalpha in MonoMac 6 cells cotransfected with a reporter gene, we provide evidence that NF-kappaB is essential for the transcriptional activation of TNF in this system
Li et al., Int Immunopharmacol 2007 (Melanoma) : Virulizin also induced the phosphorylation of IkappaB, suggesting that induction of TNFalpha expression by Virulizin is mediated by activation of NFkappaB
Kawadler et al., Cancer Biol Ther 2006 : The mechanism by which Lys63 linked polyubiquitin chains participate in signaling cascades has yet to be determined, but two recent publications ( Wu et al., Nat Cell Bio 2006 ; 8 : 398-406 and Ea et al., Mol Cell 2006 ; 22 : 245-57 ) shed light on how this distinctive modification functions in NFkappaB activation by TNFalpha ... The polyubiquitin chain on RIP1 is recognized and bound by NEMO, the regulatory subunit of the IKK complex, and this binding is essential for NFkappaB activation by TNFalpha
Weber et al., Bioorg Med Chem 2006 : Enone analogues of curcumin were compared with curcumin for their abilities to inhibit the TNFalpha induced activation of NFkappaB , using the Panomics ' NFkappaB Reporter Stable Cell Line
Yang et al., Cancer Cell 2009 (Cell Transformation, Neoplastic...) : Wu et al., in this issue of Cancer Cell, show that TNFalpha dependent NFkappaB activation induces COP9 signalosome mediated inhibition of GSK3beta and the SCF(beta-TRCP) ubiquitin ligase, thus leading to stabilization of the transcription factor Snail and promoting cell migration and metastasis
Shimada et al., J Immunol 2002 : EMSAs demonstrated that IL-17, IL-1beta, and TNF-alpha induced NF-kappaB activation within 1.5 h after stimulation, and a blockade of NF-kappaB activation by the pyrrolidine derivative of dithiocarbamate and tosyl-phe-chloromethylketone markedly reduced the IL-17-, IL-1beta-, or TNF-alpha induced IL-6 gene expression
Henkler et al., J Biol Chem 2003 : The role of tumor necrosis factor (TNF) receptor associated factor ( TRAF)-1 in NF-kappaB activation by various members of the TNF receptor family is not well understood, and conflicting data have been published
Suliman et al., FASEB J 2005 : In Wt mice, mtDNA depletion was avoided by selective iNOS blockade, and residual mtDNA loss was linked to NF-kappaB dependent TNF-alpha expression
Hirota et al., J Biol Chem 2001 : We have demonstrated that overexpression of the EGF receptor (EGFR) in NIH3T3 cells significantly enhances TNF induced NF-kappa B-dependent luciferase activity even without EGF, that EGF treatment has a synergistic effect on the induction of the reporter activity, and that this enhancement is suppressed by AG1478, EGFR-specific tyrosine kinase inhibitor ... Taken together, this evidence strongly suggests that EGFR transactivation by TNF, which is regulated in a redox dependent manner, is playing a pivotal role in TNF induced NF-kappa B activation
Lederer et al., J Immunol 1996 : We demonstrate that IL-1 and TNF induce rapid nuclear translocation of p65(RelA) in T cell clones, whereas TCR induced NF-Kappa B activation in Th1 cells is delayed and may be longer in duration
Janssen-Heininger et al., Am J Respir Cell Mol Biol 1999 : In contrast, TNF activated NF-kappaB in a Ras independent fashion ... Evaluation of members of the mitogen activated protein kinase ( MAPK ) family as downstream effectors of Ras revealed the requirement of MAPK/ extracellular regulated kinase (ERK) kinase kinase ( MEKK)1 and c-Jun N-terminal kinases in the induction of NF-kappaB by both oxidants and TNF , whereas the MEK-ERK pathway negatively regulates NF-kappaB
Hoshino et al., J Med Chem 2010 : Five resveratrol sulfate metabolites were synthesized and assessed for activities known to be mediated by resveratrol : inhibition of tumor necrosis factor (TNF) alpha induced NFkappaB activity, cylcooxygenases ( COX-1 and COX-2 ), aromatase, nitric oxide production in endotoxin stimulated macrophages, proliferation of KB or MCF7 cells, induction of quinone reductase 1 (QR1), accumulation in the sub-G ( 1 ) phase of the cell cycle, and quenching of 2,2-diphenyl-1-picrylhydrazyl ( DPPH ) free radical
Shimada et al., Dig Dis Sci 1999 : TNF-alpha induced activation of NF-kappaB activity was suppressed by NAC, and H2O2 caused significant activation of NF-kappaB
Duckett et al., J Biol Chem 1995 : Here we examine the inductions of NF-kappa B in serum deprived and cycling cells in response to tumor necrosis factor-alpha (TNF-alpha) ... These data reveal that NF-kappa B is rapidly induced by TNF-alpha in both proliferating and arrested cells and suggest that distinct activation pathways can lead to cell cycle dependent or -independent induction of NF-kappa B
Badgwell et al., Surgery 2002 (Escherichia coli Infections...) : The 2 groups of mice were analyzed for serum levels of interferon-gamma, tumor necrosis factor-alpha , and interleukin-1beta as well as activation of NFkappaB and STAT1, 2 proinflammatory transcription factors
García-Bueno et al., Psychopharmacologia 2005 : The mechanisms involved in the anti-oxidative properties of 15d-PGJ2 in stress involve NFkappaB blockade ( by preventing stress induced IkappaBalpha decrease ) as well as inhibition of TNFalpha release in stressed animals
Ouchi et al., Circulation 2000 : Adiponectin suppressed TNF-alpha induced IkappaB-alpha phosphorylation and subsequent NF-kappaB activation without affecting other TNF-alpha mediated phosphorylation signals, including Jun N-terminal kinase, p38 kinase, and Akt kinase
Farmer et al., Am J Physiol Endocrinol Metab 2000 : We tested the hypothesis that the TNF-alpha stimulated transcription factor nuclear factor (NF)-kappaB could, in part, mediate TNF-alpha action by inhibiting the transcriptional potency of the VDR and RXR at their cognate cis regulatory sites ... These results suggest that TNF-alpha inhibition of hormone stimulated transcriptional activation may be mediated by activation of NF-kappaB
Hazan et al., Proc Natl Acad Sci U S A 1990 : Stimulation of a human T-cell clone with anti-CD3 or tumor necrosis factor induces NF-kappa B translocation but not human immunodeficiency virus 1 enhancer dependent transcription ... Our finding that induction of NF-kappa B by tumor necrosis factor or antibody to CD3 is not sufficient to induce HIV enhancer dependent transcription in cloned T cells contrasts with results obtained in most lymphoblastoid T-cell lines and indicates that normal T lymphocytes differ from tumoral T cells in terms of requirements for HIV LTR activation
Manna et al., Cancer Res 2000 : Oleandrin blocked tumor necrosis factor (TNF) induced activation of NF-kappaB in a concentration- and time dependent manner ... A proprietary hot water extract of oleander ( Anvirzel ) also blocked TNF induced NF-kappaB activation ; subsequent fractionation of the extract revealed that this activity was attributable to oleandrin ... The effects of oleandrin were not cell type specific, because it blocked TNF induced NF-kappaB activation in a variety of cells
Inagaki et al., J Biol Chem 2008 : Tumor necrosis factor- and interleukin-1 induced activation of TAK1 was entirely normal in Tab1-deficient MEFs and could activate both mitogen activated protein kinases and NF-kappaB
Schwabe et al., Am J Physiol Gastrointest Liver Physiol 2002 : Role of glycogen synthase kinase-3 in TNF-alpha induced NF-kappaB activation and apoptosis in hepatocytes ... In this study, we determined the role of GSK-3 in TNF-alpha induced NF-kappaB activation and cell death in primary hepatocytes
Takada et al., J Biol Chem 2004 : TNF induced NF-kappaB activation as measured by DNA binding in wild-type mouse embryonic fibroblasts, but deletion of GSK-3beta abolished this activation ... TNF induced NF-kappaB reporter gene transcription was also suppressed in GSK-3beta gene deleted cells
Thommesen et al., J Biochem Mol Biol 2005 (Colonic Neoplasms) : Distinct differences between TNF receptor 1- and TNF receptor 2-mediated activation of NFkappaB
Kang et al., Int J Mol Med 2005 : In addition, GA inhibited TNF-alpha induced phosphorylation of p38 MAPK and extracellular regulated kinases (ERK), I kappa B alpha degradation, and NF-kappa B activation
Dekelbab et al., Steroids 2007 : The synthetic glucocorticoid, dexamethasone ( DEX ) blunted TNF-alpha stimulated NF-kappaB activation in L6 cells
Wong et al., J Clin Invest 1997 (Adenocarcinoma...) : In A549 cells induction of the stress response decreased tumor necrosis factor-alpha (TNF-alpha) mediated NF-kappaB nuclear translocation ... TNF-alpha initiates NF-kappaB nuclear translocation by causing dissociation of the inhibitory protein I-kappaBalpha from NF-kappaB and rapid degradation of I-kappaBalpha
An et al., Int Immunopharmacol 2002 : Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don ( Bignoniaceae ), was found to inhibit the productions of tumor necrosis factor-alpha (TNF-alpha) , interleukin-1beta (IL-1beta), and interleukin-6 (IL-6), and the activation of nuclear factor kappaB (NF-kappaB) in RAW 264.7 macrophages activated with lipopolysaccharide (LPS)
Wang et al., Cancer Res 2006 (Carcinoma...) : The secretory leukocyte protease inhibitor ( SLPI ) can attenuate the host proinflammatory response by blocking nuclear factor kappaB (NF-kappaB) mediated tumor necrosis factor alpha (TNF-alpha) production in macrophages
Chen et al., J Cell Biochem 2001 : SME or Sal B significantly inhibited TNF-alpha induced activation of nuclear factor kappa B (NF-kappaB) in HAECs ( 0.36- and 0.48-fold, respectively )
Habib et al., J Biol Chem 2001 : NF-kappaB activation by the tumor necrosis factor receptor (TNFR) involves the formation of a multiprotein complex termed a signalosome
Geng et al., Free Radic Biol Med 1997 : SAC consistently exhibited a dose dependent inhibition of NF-kappa B activation induced by both TNF-alpha and H2O2
Petzold et al., Am J Physiol Cell Physiol 2009 : NF-kappaB activation by TNF-alpha or hydrogen peroxide was FAK independent
Lam et al., Proc Natl Acad Sci U S A 2008 (Lymphoma, Large B-Cell, Diffuse) : In models of physiologic NF-kappaB pathway activation by CARD11 or tumor necrosis factor-alpha , compensatory IKKalpha activity was also observed with IKKbeta inhibition
Melén et al., J Med Virol 2004 : Tumor necrosis factor-alpha (TNF-alpha) induced nuclear import of NF-kappaB was only weakly or not at all inhibited, suggesting that the nuclear import machinery in general was not impaired
Ishida et al., Inflamm Bowel Dis 2010 (Colitis...) : In HEK293 cells, pretreatment with RvE1 inhibited TNF-alpha induced nuclear translocation of NF-kappaB in a ChemR23 dependent manner
Thome et al., Curr Biol 1998 : TNFR-1 recruits and assembles a signaling complex containing a number of death domain ( DD ) -containing proteins, including the adaptor protein TRADD and the serine/threonine kinase RIP, which mediates TNF induced NF-kappa B activation
Nick et al., J Clin Invest 1999 : Specific inhibitors of p38alpha MAPk blocked LPS induced adhesion, nuclear factor-kappa B (NF-kappaB) activation , and synthesis of tumor necrosis factor-alpha (TNF-alpha)
Mahoney et al., Proc Natl Acad Sci U S A 2008 : Both cIAP1 and cIAP2 regulate TNFalpha mediated NF-kappaB activation ... Whereas NF-kappaB was properly activated by TNFalpha in cultured and primary cells deficient in either cIAP1 or 2, removal of both cIAPs severely blunted its activation
Lin et al., Toxicol Appl Pharmacol 2008 : Furthermore, the involvement of NF-kappaB in TNF-alpha induced MMP-9 production was consistent with that TNF-alpha stimulated degradation of IkappaB-alpha and translocation of NF-kappaB into the nucleus which were blocked by helenalin, but not by U0126 and SP600125, revealed by immunofluorescence staining
Kaler et al., Exp Cell Res 2008 (Colonic Neoplasms) : We showed that overexpression of HDAC2 enhanced TNFalpha induced NF-kappaB activity and that silencing of HDAC2 decreased NF-kappaB activity
Mühlenbeck et al., J Biol Chem 2000 : Overexpression of the tumor necrosis factor (TNF) related apoptosis inducing ligand ( TRAIL ) receptors, TRAIL-R1 and TRAIL-R2, induces apoptosis and activation of NF-kappaB in cultured cells
Hehner et al., J Biol Chem 1998 : Tumor necrosis factor-alpha induced cell killing and activation of transcription factor NF-kappaB are uncoupled in L929 cells
Lee et al., American journal of physiology. Renal physiology 2008 (Kidney Diseases...) : Sevoflurane caused nuclear translocation of SMAD3 and reduced the TNF-alpha induced nuclear translocation of NF-kappaB in primary cultures of proximal tubules from TGF-beta1+/+ but not in TGF-beta1+/- mice
Ravindran et al., Biochem Pharmacol 2010 : We found that bisdemethylcurcumin ( BDC ) was more potent than curcumin as an anti-inflammatory agent as indicated by suppression of TNF induced NF-kappaB activation, more potent as an anti-proliferative agent, and more potent in inducing ROS
Lee et al., Cytokine 2008 (Colitis...) : Furthermore, mycophenolic acid ( MPA ) reduced TNF-alpha stimulated NF-kappaB activation in HT-29 colon epithelial cells
Jono et al., J Biol Chem 2004 (Inflammation) : The present study revealed a novel autoregulatory feedback pathway through which activation of NF-kappaB by TNF-alpha and bacterium nontypeable Haemophilus influenzae ( NTHi ) induces CYLD that in turn leads to the negative regulation of NF-kappaB signaling
Leung et al., Nephrol Dial Transplant 2008 : Furthermore, the increased synthesis of IL-6 and TNF-alpha by anionic pIgA in HMC was significantly diminished ( P < 0.01 ) in the presence of NF-kappaB inhibitor pyrrolidine dithiocarbamate and NF-kappaB blocking permeable peptides SN50 ( P < 0.01 ) ... While both the p42/p44 MAPK and NF-kappaB pathways are essential in regulating the anionic pIgA induced synthesis of IL-6, TNF-alpha synthesis mediated by anionic pIgA is partly dependent on NF-kappaB
Pfeffer et al., Mutat Res 2005 : The interference with the TNFalpha pathway is reflected by reduced NFkappaB activation in anti-oxidants treated cells but the compounds are not able to contrast TNFalpha mediated activation of NFkappaB
Dauletbaev et al., Respiration 2010 (Cystic Fibrosis) : Both TNF-alpha and IL-1beta activated NF-kappaB and stimulated IL-8 production
Yang et al., J Nutr 1998 (Disease Models, Animal) : We postulated that green tea polyphenols regulate TNFalpha gene expression by modulating NF-KB activation through their antioxidant properties
Kasperczyk et al., FASEB J 2009 (Neoplasms) : Further, NF-kappaB activation by tumor necrosis factor alpha (TNF-alpha) or p65 overexpression stimulates Shh promoter activity and p65 binds to Shh promoter in vivo
Reiterer et al., J Nutr 2004 (Inflammation) : However, if zinc was added back, all PPAR agonists significantly downregulated the TNF-alpha mediated induction of inflammatory transcription factors NF-kappaB and AP-1 and significantly reduced the expression of their target genes, VCAM-1 and IL-6
Lieb et al., J Neurochem 1996 (Astrocytoma) : Here, we describe that both IL-1 beta and TNF alpha activate the transcription factor nuclear factor-kappa B (NF-kappa B) via production of reactive oxygen intermediates resulting in ACT expression
Heynekamp et al., J Med Chem 2006 : In the present study, phenolic analogues of resveratrol and a series of substituted trans-stilbenes without hydroxy groups were compared with resveratrol for their abilities to inhibit the human tumor necrosis factor alpha induced ( TNF-alpha ) activation of NF-kappaB , using the Panomics NF-kappaB stable reporter cell line 293/NF-kappaB-luc
Aggarwal et al., J Immunol 2004 (Melanoma) : In the present report we investigated the effect of MDA-7 on NF-kappaB activation and on TNF induced NF-kappaB activation and apoptosis in human embryonic kidney 293 cells ... However, TNF induced NF-kappaB activation was significantly enhanced in mda-7 transfected cells, as indicated by DNA binding, p65 translocation, and NF-kappaB dependent reporter gene expression ... Mda-7 transfection also potentiated NF-kappaB reporter activation induced by TNF receptor associated death domain and TNF receptor associated factor-2 ... Cytoplasmic MDA-7 with deleted signal sequence was as effective as full-length MDA-7 in potentiating TNF induced NF-kappaB reporter activity ... Secretion of MDA-7 was not required for the potentiation of TNF induced NF-kappaB activation ... Overall, our results indicate that stable or transient MDA-7 expression alone does not substantially activate NF-kappaB, but potentiates TNF induced NF-kappaB activation and NF-kappaB regulated gene expression
Sadowska et al., Pharmacol Res 2006 : Neutrophils from healthy volunteers ( NAC naïve ) were pre incubated with NAC for 30 min and the effects on the release of elastase and IL-8, the respiratory burst in response to fMLP and PMA, on TNFalpha induced NFkappaB activation and on the migration across an endothelial-epithelial bilayer were investigated
Bátkai et al., Am J Physiol Heart Circ Physiol 2007 (Inflammation...) : Anandamide dose dependently attenuated the TNF-alpha induced ICAM-1 and VCAM-1 expression, NF-kappaB activation in HCAECs, and the adhesion of monocytes to HCAECs in a CB(1)- and CB(2) dependent manner
Han et al., Free Radic Biol Med 2006 : These redox alterations were found to inhibit TNF induced IkappaB-alpha phosphorylation and NF-kappaB translocation to the nucleus, thus presumably inhibiting expression of genes necessary to inhibit the cytotoxic effects of TNF
Hatano et al., Gastroenterology 2001 : Our aim was to study the role of NF-kappaB activation and inducible nitric oxide synthases ( iNOSs ) in TNF-alpha- and Fas mediated apoptosis in hepatocytes ... TNF-alpha and Jo2 induced iNOS messenger RNA and protein levels through the induction of NF-kappaB
Ohkita et al., Jpn J Pharmacol 2002 : BAY 11-7082, an inhibitor of nuclear factor-kappaB (NF-kappaB), which prevents a step of the phosphorylation of inhibitory protein IkappaB bound to NF-kappaB, suppressed basal and tumor necrosis factor (TNF)-alpha induced prepro endothelin (ET)-1 mRNA expression and NF-kappaB activation in cultured vascular endothelial cells
Itatsu et al., Liver Int 2009 (Cholangiocarcinoma) : As for the signalling pathway, TNF-alpha stimulation led to the phosphorylation of extracellular signal regulated kinase 1/2 ( Erk1/2 ) and p38 mitogen activated protein kinase ( p38MAPK ) and translocation of nuclear factor kappaB (NF-kappaB) ( p65 ) into the nuclei ... These data suggest that TNF-alpha/TNF-R1 interaction leads to the phosphorylation of Erk1/2 and p38MAPK and nuclear translocation of NF-kappaB , which is closely associated with the production and activation of MMP-9 in cultured CC cells of HuCTT-1 and CCKS-1
Oshima et al., Am J Physiol Cell Physiol 2001 (Inflammatory Bowel Diseases...) : Similarly, TNF-alpha activated NF-kappa B through TK, p42/44, p38 MAPKs, and PARP pathways in SVEC cells
Barchowsky et al., Cytokine 2000 (MAP Kinase Signaling System) : Although both IL-1 and TNF activate NF-kappaB in these cells, only IL-1 induces collagenase-1 transcription
Gerbod-Giannone et al., Proc Natl Acad Sci U S A 2006 : TNFalpha induces ABCA1 through NF-kappaB in macrophages and in phagocytes ingesting apoptotic cells
Ahn et al., Mol Pharmacol 2007 (Adenocarcinoma...) : We found that embelin inhibited tumor necrosis factor (TNF) alpha induced NF-kappaB activation
Taylor et al., J Virol 2009 : We report here that the nucleocapsid ( N ) protein of HTNV was able to inhibit TNF-alpha induced activation of NF-kappaB , as measured by a reporter assay, and the activation of endogenous p65, an NF-kappaB subunit
Campagnuolo et al., Bioorg Med Chem 2005 : Compound 3 proved active in selectively inhibiting the induction of NF-kappaB by tumor necrosis factor-alpha in T cells
Malhotra et al., Am J Respir Cell Mol Biol 2001 : Geldanamycin inhibited TNF-alpha mediated NF-kappaB activation as measured by electromobility shift assays and transient transfections with a NF-kappaB dependent luciferase reporter plasmid
Itoh et al., Biol Pharm Bull 2007 (Arthritis, Rheumatoid) : DHT inhibited the NF-kappaB activation induced by TNFalpha in a manner dependent on the androgen receptor (AR)
Vila-del Sol et al., J Immunol 2005 : Taken together our results suggest a model in which IFN-gamma induced TNF activates NF-kappaB , which is required for full COX-2 expression
Manna et al., J Immunol 2000 : These effects were specific to HIV-tat, as activation of NF-kappa B by PMA, LPS, H2O2, and TNF was minimally affected
Hong et al., J Biol Chem 2007 : Interestingly, peptides spanning CCR2 and/or LZ disrupt IKKgamma-Tax and IKKgamma-PP2A interactions and potently inhibit NF-kappaB activation by Tax and tumor necrosis factor-alpha
Qin et al., Cell Mol Immunol 2007 (Acute Disease...) : Electrophoretic mobility shift assay ( EMSA ) revealed that TNF-alpha induced NF-kappaB transactivation in AML14 cells in a time- and dose dependent fashion, and subsequent supershift assays indicated that the translocated NF-kappaB was the heterodimer p65 ( RelA)/p50
Byun et al., Exp Mol Med 2002 : In contrast, exposure to oxidative stress in certain cell types induced reduction of tumor necrosis factor (TNF)- induced NF-kappaB activation ... To understand such conflicting effect of oxidative stress on NF- kappakB activation, HeLa cells were incubated with H ( 2 ) O ( 2 ) or diamide and TNF induced expression of NF-kappaB reporter gene was measured
Kharroubi et al., Endocrinology 2004 (Insulinoma...) : Moreover, FFAs did not enhance NF-kappaB activation by TNFalpha
Nagasaki et al., Int J Cancer 2003 (Pancreatic Neoplasms) : The activation of NF-kappaB through ligand induced stimulation by tumor necrosis factor-alpha (TNF-alpha) or phorbol 12-myristate 13-acetate ( PMA ) was also inhibited by transient expression of LDOC1 in a dose dependent manner
Saliou et al., FEBS Lett 1998 : Surprisingly, tumor necrosis factor-alpha induced NF-kappaB activation was not affected by silymarin, thus demonstrating a pathway dependent inhibition by silymarin
Asakura et al., Am J Hematol 2007 : Here we show that ectopic overexpression of catalytically inactive dominant negative PKR expression system suppressed NF- kappa B activation mediated by TLR3, TLR9, TNF receptor 1 and 2 (TNF-R 1/2) , but not by TLR4
O'Connell et al., J Biol Chem 1995 (Lymphoma, T-Cell) : As the TNF gene has an NF kappa B binding motif, an autocrine loop involving TNF induction of NF kappa B is therefore likely in these cells
O'Connor et al., J Immunol 2003 : Transfection of full-length CIAS1 or either of two shorter, naturally occurring isoforms dramatically inhibited TNF-alpha induced activation of NF-kappaB reporter activity
Yang et al., Nat Immunol 2001 : The essential role of MEKK3 in TNF induced NF-kappaB activation ... Using mitogen activated protein kinase kinase kinase 3 ( MEKK3 ) -deficient fibroblast cells, we found that MEKK3 plays a critical role in TNF induced NF-kappaB activation ... The kinase activity of MEKK3 is pivotal to its function and, therefore, MEKK3 links RIP and IKK in TNF induced NF-kappaB activation
Gires et al., Cancer 2001 : TNFalpha induced inhibition of the EpCAM expression is mediated by TNF receptor 1 through the TNF receptor associated death domain protein ( TRADD ) and by the activation of nuclear factor kappaB (NF-kappaB) , and it can be blocked by dominant negative variants of TRADD and the NF-kappaB inhibitor, IkappaB
Terry Powers et al., Biochemistry 2010 : TNF receptor ubiquitous scaffolding and signaling protein (TRUSS), a 90.1 kDa TNF-R1 associated scaffolding protein, also interacts with TRAF2 and IKK and contributes to TNF-alpha induced nuclear factor-kappaB (NF-kappaB) and c-Jun-NH ( 2 ) -terminal kinase ( JNK ) activation
Carpentier et al., FEBS Lett 1999 : TRAF1 is a TNF inducible regulator of NF-kappaB activation ... Interestingly, overexpression of TRAF1 in HEK293T completely prevented NF-kappaB activation induced by TNF , IL-1, or overexpression of TRAF2 or TRAF6
Lavagno et al., Eur J Pharmacol 2004 (Arthritis, Rheumatoid) : Anti-inflammatory drugs and tumor necrosis factor-alpha production from monocytes : role of transcription factor NF-kappa B and implication for rheumatoid arthritis therapy
Sur et al., Arch Dermatol Res 2008 (Dermatitis, Contact...) : Furthermore, cells treated with avenanthramides showed a significant inhibition of tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB luciferase activity and subsequent reduction of interleukin-8 (IL-8) release
Saito et al., American journal of physiology. Renal physiology 2010 : TNF-alpha caused activation of NF-kappaB , MAP kinases, and PI3K-Akt in podocytes, whereas blockade of these molecules did not affect inhibition of RAR by TNF-alpha
Lee et al., Oncol Rep 2007 (Urinary Bladder Neoplasms) : Aqueous extract of Magnolia officinalis mediates proliferative capacity, p21WAF1 expression and TNF-alpha induced NF-kappaB activity in human urinary bladder cancer 5637 cells ; involvement of p38 MAP kinase ... Furthermore, the transactivation of TNF-alpha stimulated NF-kappaB was inhibited by SB203580 treatment
Pinkoski et al., J Biol Chem 2002 : TNFalpha induced activation of the Fas-ligand promoter in IEC requires NF-kappaB as this was blocked by an I-kappaBalphaM super-repressor and by mutation of an NF-kappaB site in the Fas-ligand promoter
Thimmulappa et al., J Clin Invest 2006 (Pneumonia...) : Nrf2 's regulation of cellular glutathione and other antioxidants is critical for optimal NF-kappaB activation in response to LPS and TNF-alpha
Chen et al., Am J Physiol Heart Circ Physiol 2006 : In contrast, Nrf2 did not inhibit TNF-alpha induced NF-kappaB activation
Tamatani et al., J Biol Chem 1999 : Tumor necrosis factor induces Bcl-2 and Bcl-x expression through NFkappaB activation in primary hippocampal neurons ... Expression of the mutant NFkappaB completely inhibited NFkappaB DNA binding activity and inhibited both TNF induced up-regulation of Bcl-2 and Bcl-x expression and neuroprotective effect
Nagaki et al., J Hepatol 1999 : GalN treatment failed to affect TNF-alpha induced NF-kappaB activation
Wesemann et al., J Immunol 2002 : We conclude that SOCS-1 inhibits cytokine induced CD40 expression by blocking IFN-gamma mediated STAT-1alpha activation, which also then results in suppression of IFN-gamma induced TNF-alpha secretion and subsequent NF-kappaB activation
Fujisawa et al., Arthritis Rheum 1996 : To examine whether nuclear factor kappaB (NF-kappaB) is activated in cultured synovial cells in response to tumor necrosis factor alpha (TNFalpha) and to investigate the correlation between NF-kappaB activation and synovial cell proliferation ... Stimulation of synovial cells with TNFalpha activated NF-kappaB and subsequent transcription of several genes ... Treatment of synovial cells with N-acetyl-L-cysteine (NAC), an antioxidant agent, inhibited TNFalpha induced NF-kappaB activation and transcription ... Our results suggest that NF-kappaB plays a pivotal role in synovial cell activation by TNFalpha
Myskiw et al., J Virol 2009 : PKR dependent activation of p38 and NF-kappaB was required for vaccinia virus induced INHBA expression, whereas induction of TNF-alpha required only PKR dependent NF-kappaB activation
Gerritsen et al., Am J Pathol 1995 (Inflammation) : Although apigenin did not inhibit TNF-alpha induced nuclear translocation of NF-kappa B ( p50 ( NFKB1 ) /p65 ( RelA ) ) we found this flavonoid did inhibit TNF-alpha induced beta-galactosidase activity in SW480 cells stably transfected with a beta-galactosidase reporter construct driven by four NF-kappa B elements, suggesting an action on NF-kappa B transcriptional activation
Usui et al., J Pharmacol Sci 2010 (Atherosclerosis) : NAC inhibited TNF induced phosphorylation of JNK and p38 but not NF-kappaB
Kurokouchi et al., J Biol Chem 2001 : To test the hypothesis that fluid flow alters NF-kappaB activation and expression of cell adhesion molecules in osteoblastic cells, we examined the effect of oscillating fluid flow ( OFF ) on tumor necrosis factor (TNF)-alpha induced NF-kappaB activation in rat osteoblast-like UMR106 cells ... The inhibitory effects of OFF on both TNF-alpha induced NF-kappaB activation and intercellular adhesion molecule-1 mRNA expression were shear stress dependent and also increased with OFF exposure duration, indicating that OFF has potent effects on mechanotransduction pathways ... Thus, OFF inhibits TNF-alpha induced IKK activation, leading to a decrease in phosphorylation and degradation of inhibitory IkappaBalpha, which in turn results in the decrease of TNF-alpha induced NF-kappaB activation and potentially the transcription of target genes
Iimuro et al., J Gastroenterol Hepatol 2007 : In Myd88 ( -/- ) mice after PH, induction of expression of immediate early genes involved in hepatocyte replication and phosphorylation of signal transducer and activators of transcription 3 ( STAT3 ) in the liver, and production of TNF-alpha/IL-6 by and activation of NF-kappaB in the Kupffer cells were grossly subnormal and were associated with impaired liver regeneration, while TLR2, 4 and 9, which recognize Gram negative and -positive bacterial products, are not essential for NF-kappaB activation and IL-6 production after PH
Li et al., FASEB J 2003 : The UbcH2 promoter region contains a functional NF-kappaB binding site ; NF-kappaB binding to this sequence is increased by TNF-alpha stimulation
Fang et al., Can J Physiol Pharmacol 2010 : In addition, PGE1 suppressed TNF induced NF-kappaB activation and production of reactive oxygen species
Wang et al., Zhong Xi Yi Jie He Xue Bao 2009 : TNF-alpha ( 1 microg/L ) strongly induced the expression of NF-kappaB by approximately 1.76-fold compared with the control in the nuclei of A549 cells, and the induced NF-kappaB expression was significantly suppressed by addition of Feiyanning ( P < 0.01 ). In addition, Feiyanning inhibited the transcriptional activity of the NF-kappaB promoter. However, we observed no significant changes in IkappaBalpha expression ( P > 0.05 ) ... Feiyanning Decoction can markedly inhibit human lung cancer A549 cell proliferation, which may be partly due to inhibition of NF-kappaB activation induced by TNF-alpha
Tay et al., Nature 2010 : We developed a stochastic mathematical model that reproduces both the digital and analogue dynamics as well as most gene expression profiles at all measured conditions, constituting a broadly applicable model for TNF-alpha induced NF-kappaB signalling in various types of cells
Wagner et al., Exp Dermatol 2010 : Furthermore, SFN elevated cellular glutathione levels and antagonized tumor necrosis factor-alpha induced NFkappaB transactivation
Haridas et al., Proc Natl Acad Sci U S A 2001 : Both F094 and avicin G were found to be potent inhibitors of TNF induced NF-kappaB
Chêne et al., J Virol 1999 : TNF is the major inducer of NF-kappaB and particularly of the p50-p65 complex, whereas IL-7 acts as a cofactor by sustaining the expression of the p75 TNF receptor
Yamit-Hezi et al., J Biol Chem 2000 : TAF(II)105, a substoichiometric coactivator subunit of TFIID, is important for activation of anti-apoptotic genes by NF-kappaB in response to the cytokine tumor necrosis factor (TNF)-alpha
Kim et al., Biochem Biophys Res Commun 2002 : In addition, merlin blocked the tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB-DNA binding mediated via the inhibition of degradation of IkappaBalpha and blocked the activation of NF-kappaB dependent transcription
Sakai et al., J Bone Miner Res 2001 : Thus, we investigated whether TNF-alpha activates a transcription factor nuclear factor kappaB (NF-kappaB) in human chondrocyte-like cells ( HCS-2/8 ) and induces the expression of genes involved in the degradation of cartilage matrix ... Thus, it is shown that the activation of p65/p50 NF-kappaB by TNF-alpha plays a cardinal role in inducing the expression of MMP-1, MMP-3, ICAM-1, and COX-2 genes, which are involved in matrix degradation and inflammatory reaction in chondrocytes, leading to chondrocytic chondrolysis
Kang et al., Stem Cells Dev 2007 : Nuclear localization of NF-kappaB in response to TNF-alpha was evident in differentiated, but not undifferentiated, SNUhES3 cells
Chen et al., Shock 2006 (Burns...) : Taken together with the TNF-alpha dependent p38 and NF-kappaB activation in primed neutrophil, we conclude that thermal injury induced priming effect of polymorphonuclear neutrophil is TNF-alpha and p38 dependent
Volanti et al., Photochem Photobiol 2002 : This was further reinforced by the demonstration that herbimycin A, a tyrosine kinase inhibitor, prevented NF-kappaB activation by photosensitization but not by TNF alpha , a cytokine known to activate NF-kappaB through an IKK dependent mechanism
Alzoghaibi et al., Am J Physiol Gastrointest Liver Physiol 2004 (Crohn Disease) : In contrast, TNF-alpha markedly increased activation of NF-kappaB in both normal and Crohn 's cells
Janbandhu et al., J Biol Chem 2010 : To study the involvement of NF-kappaB in G ( 1 ) /S phase regulation, the levels of selected transcriptional regulators were monitored following overexpression of NF-kappaB or its physiological induction by tumor necrosis factor-alpha
Frelin et al., Cell Death Differ 2008 : This short form was unable to rescue NF-kappaB activation by tumor necrosis factor-alpha (TNF-alpha) when transfected in NEMO-deficient cells
Stio et al., J Steroid Biochem Mol Biol 2007 (Crohn Disease) : At the same time, NF-kappaB protein levels present in cytoplasmic extracts decreased in the presence of TNF-alpha and increased when PBMC were incubated with TX 527 ... The results of our studies indicate that TX 527 inhibits TNF-alpha mediated effects on PBMC and the activation of NF-kappaB and that its action is mediated by Vitamin D receptor (VDR), which is activated when the cells are stimulated with TX 527
Chen et al., Arterioscler Thromb Vasc Biol 2008 : JNK inhibitor and antioxidant also effectively blocked TNF-alpha induced