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EPHB2 — TPO
Text-mined interactions from Literome
Garcia et al., Mol Cell Biol 2001
:
In these cells, intensity and duration of
TPO induced
ERK signal are controlled by several regions of the cytoplasmic domain of Mpl ... We show that the small GTPases Ras and Rap1 contribute together to
TPO induced
ERK activation in UT7-Mpl cells and that they do so by activating different Raf kinases as downstream effectors : a Ras-Raf-1 pathway is required to initiate ERK activation while Rap1 sustains this signal through B-Raf ... Indeed, ( i ) in cells expressing wild-type or mutant Mpl, TPO induced Ras and Rap1 activation correlates with early and sustained phases of ERK signal, respectively ; ( ii ) interfering mutants of Ras and Rap1 both inhibit
ERK kinase activity and ERK dependent Elk1 transcriptional activation in
response to
TPO ; ( iii ) the kinetics of activation of Raf-1 and B-Raf by TPO follow those of Ras and Rap1, respectively ; ( iv ) RasV12 mediated Elk1 activation was modulated by the wild type or interfering mutants of Raf-1 but not those of B-Raf ; ( v ) Elk1 activation mediated by a constitutively active mutant of Rap1 ( Rap1V12 ) is potentiated by B-Raf and inhibited by an interfering mutant of this kinase
Kalina et al., Exp Hematol 2001
(Leukemia, Erythroblastic, Acute...) :
As demonstrated by Western blotting, TGF-beta1 reduced the
TPO stimulated
ERK1/ERK2 and STAT5 phosphorylation in Mo-7e and HEL cells
Rojnuckarin et al., J Biol Chem 2001
:
One known pathway by which
TPO induces
ERK activation is through the association of Shc with the penultimate phosphotyrosine within the TPO receptor, Mpl ... Using BaF3 cells expressing a truncated Mpl ( T69Mpl ) as a tool to identify non-Shc/Ras dependent signaling pathways, we describe here novel mechanisms of
TPO induced
ERK activation mediated, in part, by phosphoinositide 3-kinase (PI3K) ... PKCzeta and PI3K also contribute to
TPO induced
ERK activation in MKs, confirming their physiological relevance
Kamata et al., Blood 2004
(Thrombocytopenia) :
Raf-1 is not required for megakaryocytopoiesis or
TPO induced
ERK phosphorylation
Byts et al., Neurochem Res 2006
:
In neurons,
TPO induced cell death and selectively
activated ERK1/2
Hitchcock et al., Blood 2008
(MAP Kinase Signaling System...) :
In contrast,
Tpo mediated activation of positive megakaryopoiesis regulators such as
ERK1/2 and AKT is increased in FAK-null megakaryocytes, providing a plausible explanation for the observed increases in megakaryopoiesis in these mice
Samoylenko et al., Cell Signal 2008
:
In contrast to its inhibitory effects on NGF signalling,
TPO had no effect on epidermal growth factor (EGF) stimulated
ERK phosphorylation or proliferation of PC12 cells
Hitchcock et al., Blood 2008
:
After Tpo stimulation, internalization was greatly reduced in c-Mpl Y ( 78 ) F and c-Mpl Y ( 8+78 ) F, and these cell lines also exhibited increased proliferation and increased strength and duration of Jak2, STAT5, AKT, and
ERK1/2 activation in
response to
Tpo
Kawaguchi et al., Cell Biol Int 2012
:
The phosphorylation of ERK gradually increased for 2 h after the addition of soluble FN, which suggests that activation of
ERK is
essential for the initial induction of FN-induced PPF in
UT-7/TPO
Yamada et al., Biochem Biophys Res Commun 1995
:
TPO induced tyrosine phosphorylation of
Erk1 and Erk2 proteins in a dose and time dependent manner
Rouyez et al., Mol Cell Biol 1997
:
In BAF3-mpl cells,
TPO triggered a weak and transient
ERK activation, similar to that induced in UT7-mpl delta3 cells
Ezumi et al., Eur J Biochem 1998
:
TPO by itself did not
activate ERK1 , ERK2 and protein kinase C ( PKC ), whereas TPO directly enhanced the PKC dependent activation of ERKs induced by other agonists including thrombin and phorbol esters, without affecting the PKC activation by those agonists