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CDKN1A — SP1
Pathways - manually collected, often from reviews:
Text-mined interactions from Literome
Xiao et al., J Cell Biochem 1999
:
Both
Sp1 and Sp3 are
responsible for
p21waf1 promoter activity induced by histone deacetylase inhibitor in NIH3T3 cells ... These findings provide physical and functional evidence which strongly indicated that both
Sp1 and Sp3 are
responsible for TSA induced transactivation of the murine
p21WAF1 promoter in NIH3T3 cells
Sowa et al., Cancer Res 1999
:
Sp3, but not
Sp1 ,
mediates the transcriptional activation of the
p21/WAF1/Cip1 gene promoter by histone deacetylase inhibitor
Lu et al., Mol Endocrinol 2000
(Adenocarcinoma...) :
Androgen induction of cyclin dependent kinase inhibitor
p21 gene :
role of androgen receptor and transcription factor
Sp1 complex
Pardali et al., J Biol Chem 2000
:
Role of Smad proteins and transcription factor
Sp1 in
p21 ( Waf1/Cip1 ) regulation by transforming growth factor-beta
Gartel et al., Oncogene 2000
(Adenocarcinoma...) :
Sp1 and Sp3
activate p21 ( WAF1/CIP1 ) gene transcription in the Caco-2 colon adenocarcinoma cell line ... Transfection experiments in the SL2 Drosophila cell line that lacks endogenous Sp3 activity demonstrated that
Sp1 transactivates the
p21 promoter primarily through the Sp1-2 site, while Sp3 acts through the Sp1-1 site
Gartel et al., Proc Natl Acad Sci U S A 2001
:
Because
Sp1 is
required for
p21 transcription, it is possible that Myc may down-regulate p21 transcription, at least in part, by sequestering Sp1
Koutsodontis et al., J Biol Chem 2001
(Carcinoma, Hepatocellular...) :
Sp1 plays a critical role in the transcriptional activation of the human cyclin dependent kinase inhibitor
p21 ( WAF1/Cip1 ) gene by the p53 tumor suppressor protein
Santini et al., Proc Natl Acad Sci U S A 2001
:
We find that the endogenous NFAT-1 and -2 transcription factors, major downstream targets of calcineurin, associate with Sp1 in keratinocytes in a calcineurin dependent manner, and calcineurin up-regulates
Sp1/Sp3 dependent transcription and
p21 promoter activity in synergism with NFAT1/2
Hong et al., Carcinogenesis 2002
(Breast Neoplasms) :
3,3'-Diindolylmethane ( DIM ) induces a G ( 1 ) cell cycle arrest in human breast cancer cells that is accompanied by
Sp1 mediated activation of
p21 ( WAF1/CIP1 ) expression ... Thus, our observations have uncovered an antiproliferative pathway for DIM that implicates
Sp1/Sp3 induced expression of
p21 as a target for cell cycle control in human breast cancer cells
Koutsodontis et al., Biochemistry 2002
:
Sp1 , but not Sp3,
mediated the transactivation of the
p21 promoter by the TGFbeta signaling mediator Smad3 and Smad4 proteins whereas none of the individual mutations in elements 1-6 affected the transactivation of the p21 promoter by Smad proteins in HepG2 cells
Kavurma et al., J Biol Chem 2003
:
Sp1 down-regulated
p21WAF1/Cip1 expression in SMCs. Sp1 blocked assembly of cyclin D1-Cdk4-p21WAF1/Cip1 complex formation whose integrity is critical for G1- > S transition
Schavinsky-Khrapunsky et al., Oncogene 2003
:
Role of protein kinase C and the
Sp1-p53 complex in activation of
p21 ( WAF-1 ) expression by 12-O-tetradecanoylphorbol-13-acetate in human T cells
Sakaguchi et al., J Cell Biol 2003
(Calcium Signaling) :
The resulting free
Sp1/3 transcriptionally
activated p21CIP1/WAF1 , a representative negative regulator of cell growth
Moon et al., Biochem Biophys Res Commun 2004
:
Transcription factor Sp1 mediates p38MAPK dependent activation of the
p21WAF1 gene promoter in vascular smooth muscle cells by pyrrolidine dithiocarbamate
Xue et al., FEBS Lett 2004
:
Sp1 is
involved in the transcriptional activation of p16(INK4) by
p21 ( Waf1 ) in HeLa cells
Amini et al., J Biol Chem 2004
:
Expression of p53 further enhanced the level of
Vpr-Sp1 mediated transcription activation of
p21 through the sequence spanning -84 to -74 and increased the DNA binding activity of Sp1 in the presence of Vpr. Results from glutathione S-transferase pull-down assay showed the association of Vpr with p53 in extracts containing Sp1
Kavurma et al., J Cell Biochem 2004
:
Vascular smooth muscle cell-specific
regulation of cyclin dependent kinase inhibitor
p21 ( WAF1/Cip1 ) transcription by
Sp1 is mediated via distinct cis acting positive and negative regulatory elements in the proximal p21 ( WAF1/Cip1 ) promoter ...
Sp1 overexpression
activates the
p21 ( WAF1/Cip1 ) promoter in this cell type ... Furthermore, mutation in any of these five elements is not sufficient to prevent
activation of the
p21 ( WAF1/Cip1 ) promoter by
Sp1 in endothelial cells ... These findings demonstrate cell type-specific
regulation of
p21 ( WAF1/Cip1 ) transcription by
Sp1 via distinct cis acting positive and negative regulatory elements in the proximal p21 ( WAF1/Cip1 ) promoter
Savickiene et al., Int J Biochem Cell Biol 2005
(Leukemia) :
p21 ( Waf1/Cip1 ) and FasL gene
activation via
Sp1 and NFkappaB is required for leukemia cell survival but not for cell death induced by diverse stimuli
Sakaguchi et al., Proc Natl Acad Sci U S A 2005
:
On exposure of NHK cells to either agent, S100C/A11 is transferred to nuclei, where it
induces p21WAF1/CIP1 through activation of
Sp1/Sp3 ...
Sp1 complexed with NFAT1 in high Ca2+ treated cells or with Smad3 in TGFbeta1 treated cells, but not Sp1 alone, replaced KLF16 from the p21WAF1/CIP1 promoter and transcriptionally
activated the
p21WAF1/CIP1 gene
Kweon et al., Oncogene 2007
:
A novel antioxidant 3-O-Caffeoyl-1-methylquinic acid enhances ultraviolet A-mediated apoptosis in immortalized HaCaT keratinocytes via
Sp1 dependent transcriptional activation of
p21 ( WAF1/Cip1 )
Chuang et al., J Cell Biochem 2007
:
Sp1 and Smad3 are
required for high glucose induced
p21 ( WAF1 ) gene transcription in LLC-PK1 cells ... High glucose also increased the
Sp1 dependent transcriptional activity of
p21 ( WAF1 ) gene ... We concluded that high glucose induced hypertrophy via
Sp1-Smad2/3 dependent activation of
p21 ( WAF1 ) gene transcription in LLC-PK ( 1 ) cells
Wilson et al., Mol Biol Cell 2008
:
Chromatin immunoprecipitation ( ChIP ) and sequential ChIP analyses demonstrated
Sp1 dependent binding of HDAC4 to the proximal
p21 promoter, likely directed through the HDAC4-HDAC3-N-CoR/SMRT corepressor complex
Tapias et al., Cell cycle (Georgetown, Tex.) 2008
:
CDK4, SKP2, Rad51, BRCA2 and
p21 also
activated the
Sp1 promoter ...
p21 expression from a stably integrated inducible promoter in HT1080 cells
activated Sp1 expression at the promoter and mRNA levels, but at the same time it decreased Sp1 protein levels due to the activation of Sp1 degradation
Mottet et al., Oncogene 2009
(Bone Neoplasms...) :
HDAC4 interacts with Sp1, binds and reduces histone H3 acetylation at the Sp1/Sp3 binding site-rich p21 ( WAF1/Cip1 ) proximal promoter, suggesting a key
role for
Sp1 in HDAC4 mediated repression of
p21 ( WAF1/Cip1 )
Lee et al., Mol Cancer Res 2009
(Pancreatic Neoplasms) :
Activation of p21 promoter constructs by DIM-C-pPhOCH3 required the GC-rich proximal region of the promoter, and results of RNA interference studies showed that Nur77 dependent activation of the
p21 promoter
involved interactions with
Sp1 and Sp4 but not Sp3
Chew et al., J Biol Chem 2012
:
Sulforaphane induction of
p21 ( Cip1 ) cyclin dependent kinase inhibitor expression
requires p53 and
Sp1 transcription factors and is p53 dependent
Yuan et al., American journal of physiology. Renal physiology 2013
(Diabetic Nephropathies) :
Thus TGF-ß1 induced PAI-1 and
p21 expression
involves interaction of p300/CBP with Smads and
Sp1 , and increased promoter access via p300/CBP induced H3K9/14Ac
Russo et al., Cell cycle (Georgetown, Tex.) 2013
:
We demonstrate that the rpL3 mediated
p21 upregulation
requires the specific interaction between rpL3 and
Sp1
Prowse et al., J Biol Chem 1997
:
Expression studies established that both
Sp1 and Sp3
activate the
p21 promoter, but showed that only Sp3 overexpression enhances promoter inducibility during differentiation ... These findings show that both
Sp1 and Sp3 can
contribute to the basal activity of the
p21 promoter, and establish Sp3 as a specific transcription factor involved in the induction of p21 promoter during keratinocyte differentiation
Adnane et al., Mol Cell Biol 1998
(Pancreatic Neoplasms) :
These results suggest that GGTI-298 mediated upregulation of
p21 ( WAF1/CIP1 )
involves both an increase in the amount of DNA bound
Sp1-Sp3 and enhancement of Sp1 transcriptional activity