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CA2 — LPA
Text-mined interactions from Literome
Niemeier et al., Arterioscler Thromb Vasc Biol 1999
:
In addition, we show that purified megalin/gp330, immobilized on a sensor chip, directly binds
Lp(a) in a
Ca2+ dependent manner with an affinity similar to that for LDL
Lyons et al., J Bone Miner Res 2001
(Calcium Signaling...) :
However,
LPA induced
Ca2+ signaling was blocked in MC3T3-E1 cells previously exposed to SPH
Emmert et al., Am J Physiol Cell Physiol 2004
:
In the presence or absence of
Ca2+ , thrombin or
lysophosphatidic acid (LPA) increased RhoA activity and Rhokinase activity, correlating with isometric tension development and myosin II regulatory light chain ( RLC ) phosphorylation
Nilsson et al., Lipids 2003
(Calcium Signaling...) :
Moreover,
LPA and its analogs concentration-dependently
increased [
Ca2+ ] i in HEL cells
Xu et al., Cell Calcium 2005
:
Both sarcolemmal ( SL ) Na(+)-Ca2+ exchange inhibitors ( amiloride, Ni2+ and KB-R7943 ) and Na ( + ) -H+ exchange inhibitor ( MIA ) as well as SL store operated Ca2+ channel ( SOC ) antagonists ( SK & F 96365, tyrphostin A9 and gadolinium ), unlike SL Ca2+ channel antagonists ( verapamil and diltiazem ), inhibited the
LPA induced increase in [
Ca2+ ] i ... Furthermore, phospholipase C (PLC) inhibitor ( U 73122 ) and protein kinase C ( PKC ) activator ( phorbol 12-myristate 13-acetate ) attenuated the
LPA induced increase in [
Ca2+ ] i ... In addition, ryanodine-sensitive and InsP ( 3 ) -sensitive intracellular Ca2+ pools may be associated with the
LPA induced increase in [
Ca2+ ] i
Oh et al., J Cell Biochem 2006
:
Since it is known that
lysophosphatidic acid (LPA) increases intracellular
Ca2+ , we investigated the possible role of hippocalcin in the LPA induced elevation of intracellular Ca2+
Perova et al., Int J Neuropsychopharmacol 2008
(Calcium Signaling...) :
ANOVA revealed a significant effect of diagnosis, but not gender, on [Ca2+ ] B ( F1,63=4.4, p=0.04 ) and the rate of rise ( F1,63=5.2, p=0.03 ) of
LPA stimulated
Ca2+ responses in BLCLs from patients compared with those from healthy subjects ... The results suggest more rapid
LPA stimulated
Ca2+ responses occur in BLCLs from BD-I patients compared with controls, which are probably mediated, in part, by canonical transient receptor potential type 3 ( TRPC3 ) -like channels
Perova et al., Int J Neuropsychopharmacol 2010
:
Findings that chronic ex-vivo treatment with lithium modifies
lysophosphatidic acid (LPA) stimulated
Ca2+ responses in B lymphoblast cell lines ( BLCLs ) from BD-I patients and healthy controls, and differentially decreases levels of the type-3 canonical transient receptor potential Ca2+-permeable channel in BLCLs from BD-I patients, support the view that the amelioration of these abnormalities is important in the therapeutic action of lithium ... Chronic treatment with valproate significantly attenuated
LPA stimulated
Ca2+ responses ( [ downward arrow ] 8 % : F's=9.1-9.4, d.f.=1, 9, p 's < 0.05 ) compared to vehicle in BLCLs from BD-I patients and healthy controls, similar to chronic lithium treatment ( [ downward arrow ] 8 % : F=6.2, d.f.=1, 21, p < 0.05 ), but also attenuated TG-evoked Ca2+ responses ( [ downward arrow ] 10 % to [ downward arrow ] 19 % : F's=5.5-15.5, d.f.=1, 12, p 's < 0.05 ) ... However, chronic lamotrigine treatment did not affect
LPA- or TG-stimulated
Ca2+ responses
Jalink et al., J Biol Chem 1990
:
LPA induced
Ca2+ release is also observed in various other cell types, both normal and transformed, but not in Jurkat T cells and neutrophils ... The
LPA induced
Ca2+ signal is blocked completely by tetradecanoylphorbol acetate, but is not affected by prior stimulation of the cells with Ca2 ( + ) -mobilizing agonists such as bradykinin or histamine
Saville et al., Biochem J 1994
:
Removal of both extracellular and intracellular
Ca2+ did not significantly
reduce LPA- and ET-1 stimulated tyrosine phosphorylation of pp125fak
Seckl et al., Cancer Res 1994
(Carcinoma, Small Cell...) :
We demonstrate that
1-oleoyl-lysophosphatidic acid (LPA) , like serum, induced a dose dependent
increase in intracellular
Ca2+ in the H-510, H-345, and H-69 SCLC cell lines with half maximal concentrations of 18 nM, 22 nM, and 20 nM, respectively
Inoue et al., Circ Res 1995
:
LPA ( 10 nmol/L to 100 mumol/L )
increased [Ca2+ ] i in a dose dependent manner and evoked inositol trisphosphate formation
Houchi et al., Life Sci 1995
:
LPA stimulated the efflux of
45Ca2+ from the cells in a concentration dependent manner ( 10 ( -7 ) -10 ( -5 ) M )
Tokumura et al., Am J Physiol 1994
:
The stimulatory actions of LPA were suggested to be rather specific from the following findings : 1 ) their stimulation of DNA synthesis increased with an increase in their acyl moiety ; 2 ) lysophosphatidylcholine, a neutral lysophospholipid, had no mitogenic action but was cytotoxic at high concentrations ; and 3 )
LPA induced a rapid external Ca ( 2+ ) -independent increase in intracellular
Ca2+ concentration ([Ca2+]i) in single fura 2-loaded cells that resembled the receptor mediated increases in [ Ca2+ ] i triggered by different agonists, whereas lysophosphatidylcholine provoked a slow sustained increase in [ Ca2+ ] i in an external Ca ( 2+ ) -dependent manner
Thomson et al., Mol Pharmacol 1994
:
It was subsequently determined that
LPA induced a transient
increase in intracellular
Ca2+ levels in 3T3 cells
Yashiro et al., J Biol Chem 1993
:
To determine whether other lipoproteins would complex to Lp(a) in the insoluble complexes, we mixed
Lp(a) with LDL, VLDL, or HDL3 in the
presence of
Ca2+
Okajima et al., FEBS Lett 1996
(Leukemia) :
Lysophosphatidic acid (LPA) also
increased [
Ca2+ ] i in the cels, but the maximal response was about half of that of S1P, and furthermore PTX and dibutyryl cAMP treatment hardly affected the LPA induced Ca2+ mobilization
Takemura et al., Biochem J 1996
:
cADP-ribose released Ca2+ ; this was additive with
LPA induced
Ca2+ release and vice versa in permeabilized Jurkat cells
Pietruck et al., Naunyn Schmiedebergs Arch Pharmacol 1997
:
The half-maximal
effect of
LPA on the rise in
[Ca2+ ] i was attained at 7-20 nM
Seewald et al., Atherosclerosis 1997
:
LPA ( 5 micrograms/ml ) induced an increase of cytosolic free calcium concentration ( [ Ca2+ ] i ) in the
presence and absence of extracellular
Ca2+ and markedly stimulated the Na+/H+ exchanger
Holtsberg et al., J Neurochem 1997
(Ion Channel Gating) :
The
LPA induced increase in [
Ca2+ ] i required extracellular calcium
van Dijk et al., Curr Biol 1998
:
These PLDs generate bioactive LPA from pre existing Iysophosphatidylcholine in the outer membrane leaflet, resulting in activation of G-protein coupled
LPA receptors and consequent
activation of Ras, Rho and
Ca2+ signaling pathways
Lee et al., J Biol Chem 1998
:
Lysophosphatidic acid (LPA) also increased [Ca2+ ] i, but the sustained
Ca2+ response was
inhibited by EGTA