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ARHGEF1 — RHOA

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: RHOA → ARHGEF1 (directlyIncreases, ARHGEF1 Activity, RHOA Activity)
  Evidence: LSC (also known as ARHGEF1) is a haematopoietic cell-restricted GEF for RHOA, which interacts with G protein-coupled receptor (GPCR)-associated Galpha12 and Galpha13 proteins (Fig. 5). In addition to its DH domain with GEF activity, LSC has a regulator of G protein signalling (RGS) domain, which binds to Galpha proteins and increases their intrinsic GTPase activity, thereby turning off GPCR signalling. So LSC seems to carry out two functions. First, it transduces GPCR signals from Galpha12 and G...
• KEGG Vascular smooth muscle contraction: ARHGEF1/ARHGEF11/ARHGEF12 → RHOA (protein-protein, activation)
• KEGG Regulation of actin cytoskeleton: ARHGEF1/ARHGEF12 → RHOA (protein-protein, activation)
• NCI Pathway Database Regulation of RhoA activity: p115RhoGEF (ARHGEF1) → RHOA/GDP complex (RHOA) (modification, activates) Besson et al., Genes Dev 2004*, Dubash et al., J Cell Sci 2007*, Hart et al., Science 1998*
  Evidence: assay, physical interaction
• NCI Pathway Database PAR1-mediated thrombin signaling events: RHOA/GDP complex (RHOA) → G13/GTP/p115RhoGEF complex (GNA13-ARHGEF1) (modification, collaborate)
  Moers et al., Nat Med 2003, Huang et al., J Biol Chem 2007*
  Evidence: mutant phenotype, assay
• NCI Pathway Database Thromboxane A2 receptor signaling: p115RhoGEF (ARHGEF1) → RHOA/GTP complex (RHOA) (modification, activates) Sakurada et al., Am J Physiol Cell Physiol 2001*, Harenberg et al., Eur J Immunol 2005
  Evidence: mutant phenotype, assay, other species
• NCI Pathway Database Thromboxane A2 receptor signaling: p115RhoGEF (ARHGEF1) → RHOA/GDP complex (RHOA) (modification, activates) Sakurada et al., Am J Physiol Cell Physiol 2001*, Harenberg et al., Eur J Immunol 2005
  Evidence: mutant phenotype, assay, other species
• NCI Pathway Database PAR1-mediated thrombin signaling events: p115RhoGEF (ARHGEF1) → RHOA/GTP complex (RHOA) (modification, activates) Gohla et al., J Biol Chem 1999, Majumdar et al., J Biol Chem 1999*
  Evidence: mutant phenotype, assay
• NCI Pathway Database PAR1-mediated thrombin signaling events: p115RhoGEF (ARHGEF1) → RHOA/GDP complex (RHOA) (modification, activates) Gohla et al., J Biol Chem 1999, Majumdar et al., J Biol Chem 1999*
  Evidence: mutant phenotype, assay
• NCI Pathway Database LPA receptor mediated events: p115RhoGEF (ARHGEF1) → RHOA/GDP complex (RHOA) (modification, activates) Van Leeuwen et al., J Biol Chem 2003, Fukushima et al., Proc Natl Acad Sci U S A 1998
  Evidence: assay, other species
• Reactome Reaction: RHOA → ARHGEF1 (reaction) Yamashita et al., Nat Neurosci 2003
• WikiPathways G13 Signaling Pathway: ARHGEF1 → RHOA (activation)
• WikiPathways G Protein Signaling Pathways: ARHGEF1 → RHOA (activation)
• WikiPathways Regulation of Actin Cytoskeleton: ARHGEF1 → RHOA (activation)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: RHOA — ARHGEF1 (direct interaction, enzymatic study) Bourguignon et al., J Biol Chem 2003*

Text-mined interactions from Literome

Bourguignon et al., J Biol Chem 2003 (Breast Neoplasms...) : The binding of HA to MDA-MB-231 cells stimulates p115RhoGEF mediated RhoA signaling and Rho kinase (ROK) activity, which, in turn, increases serine/threonine phosphorylation of the adaptor protein, Gab-1 ( Grb2 associated binder-1 )
Huang et al., J Biol Chem 2007 (Calcium Signaling) : Collectively, these results suggest the following : 1 ) interaction of Galpha(13) SRI with p115RhoGEF is required for G ( 13 ) -mediated RhoA activation in platelets ; 2 ) signaling through the G ( 13 ) pathway is critical for PAR1 mediated human platelet functional changes and low dose thrombin induced aggregation ; and 3 ) G ( 13 ) signaling elicits calcium mobilization in human platelets through a Rho kinase independent mechanism
Garcia et al., J Biol Chem 2009 : Expression of dominant negative p115-RhoGEF or p115RhoGEF-specific siRNA inhibited both RhoA activation and adhesion on type IV collagen, whereas a constitutively active p115RhoGEF restored the arachidonic acid stimulation in cells in which the p38 MAPK had been inhibited
Ying et al., Arterioscler Thromb Vasc Biol 2009 (Calcium Signaling) : Results showed that overexpression of PDZ-RhoGEF, but not p115-RhoGEF or leukemia associated RhoGEF (LARG), enhanced RhoA activation by angiotensin II
Peng et al., Journal of neuroinflammation 2011 : Both pharmacological inhibitors and knockdown approaches were employed to investigate the role of PKC and p115RhoGEF in TNF-a induced RhoA activation and BMEC permeability