◀ Back to EGF
EGF — SHC1
Pathways - manually collected, often from reviews:
-
OpenBEL Selventa BEL large corpus:
SHC1
→
EGF
(decreases)
Olsen et al., Cell 2006*
Evidence: We have detected 6,600 phosphorylation sites on 2,244 proteins and have determined their temporal dynamics after stimulating HeLa cells with epidermal growth factor (EGF) and recorded them in the Phosida database
-
OpenBEL Selventa BEL large corpus:
SHC1
→
EGF
(increases)
Kassenbrock et al., J Biol Chem 2002*
Evidence: Stimulation of T47D cells with epidermal growth factor (EGF) results in the activation of the intrinsic tyrosine kinases of the receptor and the phosphorylation of multiple cellular proteins including the receptor, scaffold molecules such as c-Cbl, adapter molecules such as Shc, and the serine/threonine protein kinase Akt.
-
OpenBEL Selventa BEL large corpus:
SHC1
→
EGF
(increases)
Olsen et al., Cell 2006*
Evidence: We have detected 6,600 phosphorylation sites on 2,244 proteins and have determined their temporal dynamics after stimulating HeLa cells with epidermal growth factor (EGF) and recorded them in the Phosida database
-
BioCarta role of egf receptor transactivation by gpcrs in cardiac hypertrophy:
EGF/EGF R complex (EGF-EGFR)
→
SHC/GRB2/SOS-1 complex (SOS1-GRB2-SHC1)
(modification, activates)
-
BioCarta egf signaling pathway:
EGF/EGF-R complex (EGF-EGFR)
→
SHC/GRB2/SOS-1 complex (SOS1-GRB2-SHC1)
(modification, activates)
-
BioCarta keratinocyte differentiation:
EGF/EGF-R complex (EGF-EGFR)
→
SHC/GRB2/SOS-1 complex (SOS1-GRB2-SHC1)
(modification, activates)
-
NCI Pathway Database EGFR-dependent Endothelin signaling events:
EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1)
→
mTOR (MTOR)
(modification, activates)
Iwasaki et al., Endocrinology 1999
Evidence: mutant phenotype, other species
-
NCI Pathway Database EGFR-dependent Endothelin signaling events:
EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1)
→
EGF/EGFR dimer/SHC complex (EGF-EGFR-SHC1)
(modification, collaborate)
Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
Evidence: physical interaction, other species
-
NCI Pathway Database EGFR-dependent Endothelin signaling events:
EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1)
→
GRB2/SOS1 complex (GRB2-SOS1)
(modification, collaborate)
Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
Evidence: physical interaction, other species
-
NCI Pathway Database EGFR-dependent Endothelin signaling events:
EGF/EGFR dimer/SHC complex (EGF-EGFR-SHC1)
→
GRB2/SOS1 complex (GRB2-SOS1)
(modification, collaborate)
Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
Evidence: physical interaction, other species
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF/p46 SHC complex (EGFR-EGF-SHC1)
→
EGFR/EGFR/EGF/EGF complex (EGFR-EGF)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Burke et al., Mol Biol Cell 2001
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF/p46 SHC complex (EGFR-EGF-SHC1)
→
p46 SHC (SHC1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Burke et al., Mol Biol Cell 2001
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF complex (EGFR-EGF)
→
p46 SHC (SHC1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Burke et al., Mol Biol Cell 2001
Evidence: assay, physical interaction
-
NCI Pathway Database EGFR-dependent Endothelin signaling events:
EGF/EGFR dimer complex (EGF-EGFR)
→
EGF/EGFR dimer/SHC complex (EGF-EGFR-SHC1)
(modification, collaborate)
Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
-
NCI Pathway Database EGFR-dependent Endothelin signaling events:
EGF/EGFR dimer complex (EGF-EGFR)
→
SHC (SHC1)
(modification, collaborate)
Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
-
NCI Pathway Database EGFR-dependent Endothelin signaling events:
EGF/EGFR dimer/SHC complex (EGF-EGFR-SHC1)
→
SHC (SHC1)
(modification, collaborate)
Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
-
NCI Pathway Database EGFR-dependent Endothelin signaling events:
None
→
EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1)
(modification, collaborate)
Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
Evidence: mutant phenotype, other species
-
NCI Pathway Database EGFR-dependent Endothelin signaling events:
HRAS/GDP complex (HRAS)
→
EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1)
(modification, collaborate)
Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
Evidence: mutant phenotype, other species
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF complex (EGFR-EGF)
→
p52 SHC (SHC1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Burke et al., Mol Biol Cell 2001, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF complex (EGFR-EGF)
→
EGFR/EGFR/EGF/EGF/p52 SHC complex (EGFR-EGF-SHC1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Burke et al., Mol Biol Cell 2001, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
p52 SHC (SHC1)
→
EGFR/EGFR/EGF/EGF/p52 SHC complex (EGFR-EGF-SHC1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Burke et al., Mol Biol Cell 2001, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
None
→
EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999
Evidence: assay
-
NCI Pathway Database Internalization of ErbB1:
RAS family/GDP complex (HRAS_KRAS_HRAS_KRAS_NRAS)
→
EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999
Evidence: assay
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
→
RAS family/GTP complex (HRAS_KRAS_HRAS_KRAS_NRAS)
(modification, activates)
Haugh et al., J Biol Chem 1999
Evidence: assay
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
→
None
(modification, activates)
Haugh et al., J Biol Chem 1999
Evidence: assay
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF/p46 SHC complex (EGFR-EGF-SHC1)
→
GRB2/SOS1 complex (GRB2-SOS1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF/p46 SHC complex (EGFR-EGF-SHC1)
→
EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
GRB2/SOS1 complex (GRB2-SOS1)
→
EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
None
→
EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999
Evidence: assay
-
NCI Pathway Database Internalization of ErbB1:
RAS family/GDP complex (HRAS_KRAS_HRAS_KRAS_NRAS)
→
EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999
Evidence: assay
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
→
None
(modification, activates)
Haugh et al., J Biol Chem 1999
Evidence: assay
-
NCI Pathway Database Internalization of ErbB1:
GRB2/SOS1 complex (GRB2-SOS1)
→
EGFR/EGFR/EGF/EGF/p52 SHC complex (EGFR-EGF-SHC1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
GRB2/SOS1 complex (GRB2-SOS1)
→
EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
Evidence: assay, physical interaction
-
NCI Pathway Database Internalization of ErbB1:
EGFR/EGFR/EGF/EGF/p52 SHC complex (EGFR-EGF-SHC1)
→
EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1)
(modification, collaborate)
Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
Evidence: assay, physical interaction
-
Reactome Reaction:
EGF
→
SHC1
(indirect_complex)
Sordella et al., Science 2004, Greulich et al., PLoS Med 2005, Han et al., Cancer Biol Ther 2006, Grandal et al., Carcinogenesis 2007*, Huang et al., Proc Natl Acad Sci U S A 2007, Xu et al., Br J Cancer 2007, Kaushansky et al., Chem Biol 2008, VanderKuur et al., J Biol Chem 1995*, Soler et al., Oncogene 1994, Segatto et al., Oncogene 1993, Cohen et al., J Biol Chem 1996, Pinkas-Kramarski et al., EMBO J 1996, Sakaguchi et al., Mol Endocrinol 1998*
-
Reactome Reaction:
EGF
→
SHC1
(reaction)
Sordella et al., Science 2004, Greulich et al., PLoS Med 2005, Han et al., Cancer Biol Ther 2006, Grandal et al., Carcinogenesis 2007*, Huang et al., Proc Natl Acad Sci U S A 2007, Xu et al., Br J Cancer 2007, Kaushansky et al., Chem Biol 2008, VanderKuur et al., J Biol Chem 1995*, Soler et al., Oncogene 1994, Segatto et al., Oncogene 1993, Cohen et al., J Biol Chem 1996, Pinkas-Kramarski et al., EMBO J 1996, Sakaguchi et al., Mol Endocrinol 1998*
Text-mined interactions from Literome
Takeuchi et al., Am J Physiol 1999
:
Since Ras activation requires the assembly of a multiprotein complex, we examined the
effect of carbachol and
EGF on tyrosyl phosphorylation of
Shc and its association with Grb2 and the guanine nucleotide exchange factor Sos
Wang et al., J Biol Chem 1999
:
The tyrphostin AG1478, known to inhibit EGF receptor phosphorylation, also inhibited the angiotensin II and
EGF induced activation of ERK, the phosphorylation of the EGF receptor, and the subsequent association of
Shc and Grb2 ... Nitric oxide did not affect either EGF receptor phosphorylation or
Shc-Grb2 activation
induced by either Ang II or
EGF
Wu et al., DNA Cell Biol 1999
(Brain Neoplasms...) :
Kinase-deficient erbB proteins reduced
epidermal growth factor (EGF) induced tyrosine phosphorylation of endogenous
Shc proteins and also reduced immediate and sustained EGF induced ERK MAPK activities in human glioblastoma cells, although basal ERK MAPK activities were unaffected
Katayama et al., Jpn J Cancer Res 1999
(Glioma) :
By contrast,
Shc and Grb2 were inducibly associated with the EGFR in
response to
EGF stimulation of glioma cells
Jackson et al., Clin Cancer Res 2000
(Breast Neoplasms...) :
Despite reports that p66
Shc can negatively
regulate epidermal growth factor signaling to the mitogen activated protein kinase pathway, we found no change in epidermal growth factor stimulated activation of mitogen activated protein kinase in the F-11 cell line
Slack et al., Biochem J 2000
:
In co-stimulation experiments, carbachol and EGF activated MAPK in a non-additive fashion ; moreover,
EGF induced association of
Shc with the phosphorylated EGF receptor was inhibited by carbachol
Wosikowski et al., Biochim Biophys Acta 2000
(Breast Neoplasms) :
Defects in the EGFR signaling pathway cascade were found in all examined drug resistant sublines, including altered
EGF induced
Shc , Raf-1, or mitogen activated protein kinase phosphorylation
Sakaguchi et al., Biochem Biophys Res Commun 2001
:
Alpha-adaptin bound to wild-type
Shc in an
EGF dependent manner, whereas EGF dependent association of alpha-adaptin with Shc FA was markedly reduced ... These results suggest that
EGF stimulates
Shc-AP2 complex formation and association of Shc-AP2 complexes with EGF receptors
Ugi et al., J Biol Chem 2002
:
In the current studies, we find that
epidermal growth factor (EGF) and platelet derived growth factor ( PDGF )
lead to rapid phosphorylation of
Shc ( maximum at 1-2 min ), whereas insulin mediated Shc phosphorylation is relatively delayed ( maximum at 5-10 min ), suggesting that an intermediary step may be necessary for insulin stimulation of Shc phosphorylation ... In summary, 1 ) PI 3-kinase is a necessary early step in insulin stimulated Shc phosphorylation, whereas the
effects of
EGF and PDGF on
Shc phosphorylation are independent of PI 3-kinase
Tyagi et al., Oncogene 2003
(Adenocarcinoma...) :
Pretreatment of serum starved cells with GSE resulted in 70 % to almost complete inhibition of EGF induced EGFR activation and 50 % to complete inhibition of Shc activation, which corroborated with a comparable decrease in
EGF induced
Shc binding to EGFR
Guren et al., J Cell Physiol 2003
:
In hepatocytes at 24 h of culturing ( mid/late G ( 1 ) ) with 20,000 cells per cm ( 2 ),
EGF induced strong phosphorylation of the EGF receptor, as well as
Shc and ERK, and stimulated DNA synthesis, but did not activate Stat5b, although the Stat5b response to GH or PRL was retained
Ugi et al., Mol Cell Biol 2004
:
We have reported that the expression of small t antigen inhibits PP2A association with Shc, leading to augmentation of insulin and
epidermal growth factor induced
Shc phosphorylation with enhanced activation of the Ras/MAP kinase pathway
Huang et al., Oncogene 2006
(Breast Neoplasms) :
Both PRL and EGF caused robust signaling in T47D cells ; PRL acutely activated JAK2, signal transducer and activator of transcription-5 ( STAT5 ), and extracellular signal regulated kinase-1 and -2 ( ERK1 and ERK2 ), whereas
EGF caused EGFR activation and consequent
src homology collagen (SHC) activation and ERK activation
Kinoshita-Kikuta et al., Mol Cell Proteomics 2007
:
The time course changes in the
epidermal growth factor induced phosphorylation levels of
Shc and MAPK in A431 cells were visualized as highly up-shifted migration bands by subsequent immunoblotting with anti-Shc and anti-MAPK antibodies
Harrington et al., Obesity (Silver Spring) 2007
:
Src inhibitors PP1 and PP2 blocked ERK and p38 activation by low but not high EGF, and only high
EGF increased
Shc phosphorylation
Baer et al., Eur J Cell Biol 2009
(MAP Kinase Signaling System) :
EGF caused robust phosphorylation of
SHC and ERK1/2, which could be inhibited by EGF receptor antagonist AG1478 and MEK inhibitor PD98059
Chahdi et al., Cell Signal 2010
:
Endothelin-1
induces p66Shc activation through
EGF receptor transactivation : Role of beta ( 1 ) Pix/Galpha ( i3 ) interaction ... Specific inhibition of
EGF receptor by AG1478
blocked ET-1 induced
p66Shc activation and the binding of p66Shc and Galpha ( i3 ) to beta ( 1 ) Pix
Polk et al., Gastroenterology 1995
:
Tyrosine phosphorylation of the EGF receptor and
Shc were rapidly
increased by
EGF ...
EGF induced association of
Shc with the adapter protein Grb2 may have implications for activation of the p21ras signaling pathway in the intestine ... The
EGF induced membrane association of
Shc with two other phosphotyrosyl proteins suggests involvement of Shc in additional aspects of EGF-receptor signaling in the intestine
Ohmichi et al., J Biol Chem 1994
(Pituitary Neoplasms) :
EGF stimulated the tyrosine phosphorylation of three
Shc proteins and their subsequent association with its receptor
de Vries-Smits et al., Oncogene 1995
:
Also, induction of mSOS phosphorylation prior to complex formation did not affect
EGF induced association of mSOS with
Shc significantly, and Shc still associated predominantly with the faster migrating form of mSOS
Soler et al., Oncogene 1994
:
These results indicate that potent
EGF induced
SHC tyrosine phosphorylation can be triggered in cells having relatively few receptors
Hashimoto et al., Oncogene 1994
:
Both NGF and
EGF induced rapid tyrosine phosphorylation of
Shc and its association with both the receptors and with Grb2/Ash
Ohmichi et al., J Biol Chem 1994
:
Both NGF and
EGF stimulated the tyrosine phosphorylation of
Shc proteins and their subsequent association with the receptors
McNeil et al., Biochem Biophys Res Commun 1996
:
In the current study
EGF increased the tyrosine phosphorylation of the intracellular signalling protein
Shc in CHO11 cells but did not activate either of the downstream signalling enzymes raf or mitogen activated protein kinase ( MAPK )
Holt et al., J Biol Chem 1996
:
Although
EGF stimulation of the mutant EGF receptor
activates ERK and phosphorylation of both
Shc and SOS, it fails to directly associate with either Shc or Grb2
Sasaoka et al., J Biol Chem 1996
:
EGF stimulated
Shc phosphorylation as well as the formation of wild type EGFR.Shc complexes ... In contrast,
EGF stimulated
Shc phosphorylation without formation of Delta973-EGFR.Shc complexes
Mainiero et al., J Cell Biol 1996
:
Immunoprecipitation experiments indicated that stimulation with
EGF does not
result in association of alpha6beta4 with
Shc
Ricketts et al., J Biol Chem 1996
:
Functional
roles of the
Shc phosphotyrosine binding and Src homology 2 domains in insulin and
epidermal growth factor signaling
Gotoh et al., Mol Cell Biol 1997
:
Epidermal growth factor (EGF) induces tyrosine phosphorylation of the
Shc adapter protein, which plays an important role in EGF stimulated mitogenesis
Saso et al., Gastroenterology 1997
:
Grb2 binding to EGFR was suppressed, but
EGF induced Shc tyrosine phosphorylation and Grb2 association with
Shc were not significantly decreased
Bedrin et al., J Cell Physiol 1997
(Second Messenger Systems) :
In the nontransformed intestinal cell line IEC-6, expression of EGFR,
Src homology and collagen protein (SHC) , phospholipase C gamma 1 (PLC gamma), and their tyrosine phosphorylation in
response to
EGF was assayed by immunoblot ...
EGF led to an increase in the CSK association of
SHC at both states of confluence and was greater at confluence
Sharma et al., Mol Cell Biol 1997
:
However,
epidermal growth factor induced
Shc and MAPK phosphorylation was unaffected by the overexpressed proteins
Tiganis et al., Mol Cell Biol 1998
:
However, TC45 inhibited the
EGF induced association of
p52Shc with Grb2, which was attributed to the ability of the PTP to recognize specifically p52Shc phosphorylated on Y239
Deb et al., J Biol Chem 1998
:
Dominant negative forms of SHP-2 had no effect on
EGF stimulated interaction of GRB2 with EGFR or
SHC , nor did they influence phosphorylation of SHC and SHC/EGFR association
Gresham et al., Int J Cancer 1998
(Prostatic Neoplasms) :
In all of the tumor cell lines,
EGF caused an association between
Shc and Grb2, another adapter protein linked to cellular ras activation
Walker et al., Mol Cell Biol 1998
:
Analysis of the early signalling events by the WT, V741G, and Y740F mutant EGF receptors indicated that
EGF stimulates comparable levels of
Shc phosphorylation, Shc-GRB-2 association, and activation of Ras, B-Raf, and Erk-1
Banerjee et al., Alcohol Clin Exp Res 1998
(Carcinoma, Hepatocellular...) :
In contrast, ethanol treatment had no effect on
epidermal growth factor stimulated tyrosyl phosphorylation of
Shc