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ARHGEF7 — RAC1

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: RAC1 → ARHGEF7 (directlyIncreases, ARHGEF7 Activity, RAC1 Activity)
  Evidence: Rac1 activation by EGF was completely eliminate in cells transfected with pSUPER-beta-Pix (Fig. 2C). This result demonstrates that beta-Pix is an essential GEF for Rac1. # beta-PIX = ARHGEF7
• KEGG Regulation of actin cytoskeleton: ARHGEF6/ARHGEF7 → RAC1/RAC2/RAC3 (protein-protein, activation)
• NCI Pathway Database Integrin-linked kinase signaling: RAC1/GDP complex (RAC1) → ILK/betaPIX/PKL complex (ILK-ARHGEF7-GIT2) (modification, collaborate)
  Boulter et al., FASEB J 2006*
  Evidence: mutant phenotype, assay
• NCI Pathway Database Signaling events mediated by focal adhesion kinase: RAC1/GTP complex (RAC1) → PAK1/betaPIX/PKL/NCK1-2 complex (PAK1-ARHGEF7-GIT2-NCK2_NCK1) (modification, activates)
  Brown et al., Mol Biol Cell 2002
  Evidence: mutant phenotype, assay
• NCI Pathway Database Signaling events mediated by focal adhesion kinase: RAC1/GTP complex (RAC1) → RAC1/GTP/betaPIX complex (RAC1-ARHGEF7) (modification, collaborate)
  Chang et al., Mol Biol Cell 2007*
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Signaling events mediated by focal adhesion kinase: RAC1/GTP complex (RAC1) → betaPIX (ARHGEF7) (modification, collaborate) Chang et al., Mol Biol Cell 2007*
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Signaling events mediated by focal adhesion kinase: RAC1/GTP/betaPIX complex (RAC1-ARHGEF7) → betaPIX (ARHGEF7) (modification, collaborate) Chang et al., Mol Biol Cell 2007*
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Regulation of RAC1 activity: RAC1/GDP complex (RAC1) → betaPIX (ARHGEF7) (modification, collaborate) ten Klooster et al., J Cell Biol 2006*, Matsuda et al., FEBS Lett 2008*, Teng et al., J Cell Sci 2009*
  Evidence: assay
• Reactome Reaction: RAC1 → ARHGEF7 (indirect_complex) Zhang et al., J Cell Biol 2003
• Reactome Reaction: RAC1 → ARHGEF7 (reaction) Harrington et al., J Neurosci 2002, Bokoch et al., Annu Rev Biochem 2003, Zhang et al., J Cell Biol 2003, Zhang et al., J Neurosci 2005
• WikiPathways Regulation of Actin Cytoskeleton: ARHGEF6/ARHGEF7 → RAC1/RAC3/RAC2 (activation)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Hprd Interaction: ARHGEF7 — RAC1 (in vitro) Shin et al., J Biol Chem 2004*
• IRef Intact Interaction: ARHGEF7 — RAC1 (direct interaction, pull down) ten Klooster et al., J Cell Biol 2006*
• IRef Intact Interaction: ARHGEF7 — RAC1 (physical association, pull down) ten Klooster et al., J Cell Biol 2006*

Text-mined interactions from Literome

ten Klooster et al., J Cell Biol 2006 : Targeting and activation of Rac1 are mediated by the exchange factor beta-Pix ... In addition, the Rac1-beta-Pix interaction is required for Rac1 activation by beta-Pix as well as for Rac1 mediated spreading ... Finally, using cells deficient for the beta-Pix binding kinase Pak1, we show that Pak1 regulates the Rac1-beta-Pix interaction and controls cell spreading and adhesion induced Rac1 activation ... These data provide a model for the intracellular targeting and localized activation of Rac1 through its exchange factor beta-Pix
Chang et al., Mol Biol Cell 2007 : These data indicate that FAK has a role in the activation and focal adhesion translocation of Rac1 through the tyrosine phosphorylation of betaPIX
Birukova et al., J Cell Physiol 2007 : These results show for the first time that Tiam1 and betaPIX mediate OxPAPC induced Rac activation, cytoskeletal remodeling, and barrier protective response in pulmonary endothelium
Jones et al., Mol Cell Biol 2007 : Furthermore, siRNA depletion of PLCgamma1, beta-Pix , or GIT1 inhibited Cdc42 and Rac1 activation, while constitutively active forms of Cdc42 or Rac1, but not RhoA, were able to rescue the elongation of these cells
Matsuda et al., FEBS Lett 2008 : In C2C12-affixin cells, an increased level of activated Rac1 but not Cdc42 was observed, and mutant betaPIX lacking guanine nucleotide exchange factor activity inhibited lamellipodium formation
Castro-Castro et al., EMBO J 2011 : In addition to those steps, we show here that optimal Rac1 activation during cell signalling requires the engagement of a downstream, cytoskeletal based feedback loop nucleated around the cytoskeletal protein coronin 1A and the Rac1 exchange factor ArhGEF7
Bastos et al., J Cell Biol 2012 : CYK4 inhibits Rac1 dependent PAK1 and ARHGEF7 effector pathways during cytokinesis
Zheng et al., J Biol Chem 1994 : These findings suggest that PI 3-kinase, through the Rho-GAP homology domain of p85, can couple to the effector domain of Cdc42Hs and that p85 may be a target for the GTP bound forms of Cdc42Hs and Rac1