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BRAF — PRKACB
Text-mined interactions from Literome
MacNicol et al., J Biol Chem 1999
:
The kinase domains of Raf-1 and B-Raf are highly conserved, and it has been predicted that
B-Raf catalytic activity would also be
inhibited by
PKA ... We report that nerve growth factor stimulated
B-Raf activity is not
inhibited by
PKA ... By contrast, an N-terminally truncated, constitutively active form of
B-Raf is
inhibited by
PKA both in vitro and in transfected PC12 cells
Ji et al., Mol Cell Biol 2005
(Second Messenger Systems) :
In NC cultures, high-level activation of cAMP signaling mediates
protein kinase A (PKA) dependent
Rap1-B-Raf-ERK1/2 activation, leading to cytoplasmic accumulation of phospho-Smad1, thus terminating bone morphogenetic protein 2 (BMP2) induced sympathoadrenal cell development
Calipel et al., J Biol Chem 2006
(Cell Transformation, Neoplastic...) :
Pharmacological inhibition of cAMP dependent
protein kinase (PKA) and siRNA mediated depletion of PKA greatly
reduced B-Raf activity, ERK1/2 activation, and cell proliferation in ( WT ) B-Raf cells, whereas it did not affect ( V600E ) B-Raf cells, demonstrating a key role of PKA in mediating ( WT ) B-Raf/ERK signaling for uveal melanoma cell growth ... Pharmacological inhibition of cAMP dependent protein kinase (PKA) and siRNA mediated depletion of PKA greatly reduced B-Raf activity, ERK1/2 activation, and cell proliferation in ( WT ) B-Raf cells, whereas it did not affect ( V600E ) B-Raf cells, demonstrating a key
role of
PKA in mediating ( WT )
B-Raf/ERK signaling for uveal melanoma cell growth ... This ruled out a role for Rap1 in the
PKA mediated
B-Raf/ERK activation in ( WT ) B-Raf cells
Spirli et al., Hepatology 2012
(Cysts...) :
These effects are consistent with the ability of Raf inhibitors to transactivate Raf-1 when a PKA activated Ras promotes
Raf-1/B-Raf heterodimerization, and are
inhibited by interfering with
cAMP/PKA signaling both in vitro and in vivo, as shown by the reduction of liver cysts in mice treated with combined octreotide and sorafenib
Peraldi et al., FEBS Lett 1995
:
Hence, these data indicate that the PKA mediated phosphorylation of B-Raf hampers its interaction with p21ras, which is responsible for the
PKA mediated decrease in
B-Raf activity