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RELA — SPP1

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Philip et al., J Biol Chem 2003 : However, the molecular mechanism by which OPN activates NF kappa B and regulates pro-MMP-2 activation in murine melanoma ( B16F10 ) cells is not well defined ... We also investigated the mechanism of action of curcumin ( diferulolylmethane ) on OPN induced NF kappa B-mediated activation of pro-MMP-2 in B16F10 cells ... OPN also induces the nuclear accumulation of NF kappa B p65, NF kappa B-DNA binding, and transactivation ... Moreover, our data revealed that curcumin inhibited the OPN induced translocation of p65, NF kappa B-DNA binding, and NF kappa B transcriptional activity ... To our knowledge, this is the first report that OPN induces NF kappa B activity through phosphorylation and degradation of I kappa B alpha by activating IKK that ultimately triggers the activation of pro-MMP-2 and further demonstrates that curcumin potently suppresses OPN induced cell migration, tumor growth, and NF kappa B-mediated pro-MMP-2 activation by blocking the IKK/I kappa B alpha signaling pathways
Das et al., J Biol Chem 2003 (Breast Neoplasms) : However, the molecular mechanism by which upstream kinases regulate the OPN induced NFkappaB activation and urokinase type plasminogen activator ( uPA ) secretion in human breast cancer cells is not well defined ... OPN also induces NFkappaB activation through phosphorylation and degradation of IkappaBalpha by inducing the IKK activity ... However, both pharmacological ( wortmannin and LY294002 ) and genetic ( Deltap85 ) inhibitors of PI 3'-kinase inhibited OPN induced Akt phosphorylation, IKK activity, and NFkappaB activation through phosphorylation and degradation of IkappaBalpha ... To our knowledge, this is first report that OPN induces NFkappaB activity and uPA secretion by activating PI 3'-kinase/Akt/IKK mediated signaling pathways and further demonstrates a functional molecular link between OPN induced PI 3'-kinase dependent Akt phosphorylation and NFkappaB mediated uPA secretion, and all of these ultimately control the motility of breast cancer cells
Rangaswami et al., J Biol Chem 2004 (MAP Kinase Signaling System...) : OPN induced NIK dependent NFkappaB activation through ERK/IKKalpha/beta mediated pathways ... Wild type NIK, IKKalpha/beta, and ERK1/2 enhanced and kinase negative NIK ( mut NIK ), dominant negative IKKalpha/beta ( dn IKKalpha/beta ), and dn ERK1/2 suppressed the OPN induced NFkappaB activation , uPA secretion, pro-MMP-9 activation, cell motility, and chemoinvasion ... To our knowledge, this is the first report that NIK plays a crucial role in OPN induced NFkappaB activation , uPA secretion, and pro-MMP-9 activation through MAPK/IKKalpha/beta mediated pathways, and all of these ultimately control the cell motility, invasiveness, and tumor growth
Renault et al., J Biol Chem 2005 : Using dominant negative mutants of IkappaB kinase and USF transcription factors, we confirmed that NFkappaB and USF-1/USF-2 are involved in the UTP mediated expression of OPN
Rangaswami et al., J Biol Chem 2005 (Neoplasm Invasiveness) : We have recently demonstrated that nuclear factor inducing kinase ( NIK ) plays a crucial role in osteopontin (OPN) induced mitogen activated protein kinase/I kappa B alpha kinase dependent nuclear factor kappa B (NF kappa B)-mediated promatrix metalloproteinase-9 activation ( Rangaswami, H., Bulbule, A., and Kundu, G. C. ( 2004 ) J. Biol. Chem. 279, 38921-38935 )
Xu et al., J Clin Invest 2005 (Arthritis, Rheumatoid...) : Furthermore, it was evident that OPN activated transcription factor NF-kappaB in mononuclear cells
Das et al., IUBMB Life 2005 (Disease Progression...) : However, the molecular mechanism by which upstream kinases regulate the OPN induced NFkappaB activation and uPA secretion in human breast cancer cells is not well defined ... OPN also induces NFkappaB activation through phosphorylation and degradation of IkappaBalpha by inducing the IKK activity ... Taken together, OPN induces NFkappaB activity and uPA secretion by activating PI 3'-kinase/Akt/IKK mediated signaling pathways and further demonstrates a functional molecular link between OPN induced PI 3'-kinase dependent Akt phosphorylation and NFkappaB mediated uPA secretion, and all of these ultimately control the motility and invasiveness of breast cancer cells
Rangaswami et al., Glycoconj J 2006 (Melanoma) : OPN induces NIK dependent NF-kappaB activation through ERK/IKKalpha/beta mediated pathways
Rice et al., J Vasc Res 2006 : OPN mediated NF-kappaB activity is increased upon NIK wild-type ( WT ) overexpression and blocked following NIK DN overexpression
Samant et al., Molecular cancer 2007 (Breast Neoplasms) : Breast cancer metastasis suppressor 1 ( BRMS1 ) inhibits osteopontin transcription by abrogating NF-kappaB activation ... Our results show that BRMS1 regulates OPN transcription by abrogating NF-kappaB activation
Zhao et al., Gastroenterology 2008 (Liver Neoplasms...) : Furthermore, down-regulation of osteopontin inhibited drug induced NF-kappaB activation and sensitized HCC cells to chemotherapeutic agents in vitro, which led to complete regression of HCC xenografts in nude mice
Ahmed et al., Molecular cancer 2010 (Breast Neoplasms) : The data revealed that OPN induces NF-kappaB activation and NF-kappaB dependent ICAM-1 expression ... We also observed that OPN induced NF-kappaB further controls AP-1 transactivation, suggesting that there is cross talk between NF-kappaB and AP-1 which is unidirectional towards AP-1 that in turn regulates ICAM-1 expression in these cells ... Moreover, overexpression of mTOR inhibits OPN induced NF-kappaB and AP-1-DNA binding and transcriptional activity
Shatrov et al., Biochem Biophys Res Commun 1997 (Leukemia, Myeloid) : In addition, SPP induced NF-kappa B activation was blocked both by cyclosporin A, known to inhibit calcineurin phosphatase activity, and by the antioxidant butylated hydroxyanisole ... These observations suggest that intracellular calcium mobilization is required for SPP induced NF-kappa B activation, which may involve calcineurin- and redox dependent mechanisms
Scatena et al., J Cell Biol 1998 : The alphavbeta3 integrin was most important for osteopontin mediated NF-kappaB induction and survival, since adding a neutralizing anti-beta3 integrin antibody blocked NF-kappaB activity and induced endothelial cell death when cells were plated on osteopontin ... Activation of NF-kappaB by osteopontin depended on the small GTP binding protein Ras and the tyrosine kinase Src, since NF-kappaB reporter activity was inhibited by Ras and Src dominant negative mutants ... In contrast, inhibition of MEK and PI3-kinase did not affect osteopontin induced NF-kappaB activation