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EGF — PRL
Text-mined interactions from Literome
Goda et al., Endocr J 1998
:
We also labeled MtT/S cells with BrdU for 24 h during
PRL producing cell
induction by insulin and
EGF , and as a result BrdU labeled proliferative cells were specifically absent from PRL producing cell populations
Jacob et al., Mol Cell Endocrinol 1999
:
Epidermal growth factor (EGF) increases
prolactin gene expression in GH4 cells, but the promoter element ( s ) required for this response has not been clearly defined ... However, the effects of EGF are additive with the responses to insulin or cAMP implying that
EGF activated
prolactin gene transcription by a mechanism different from insulin or cAMP
McManaman et al., Arch Biochem Biophys 2000
:
Prolactin and cortisol
increased XOR protein and mRNA in the presence of
epidermal growth factor , which blocked the stimulation of beta-casein synthesis by these hormones
Sakamoto et al., Horm Res 2000
:
We investigated the
effects of
EGF on endometrial cell viability, 8-Br-cAMP induced stromal decidualization, and
prolactin secretion from decidualized endometrial stromal cells using an in vitro decidualization activity assay of human endometrial stromal cells ...
Prolactin release from the 8-Br-cAMP induced decidualized cells was not
affected by
EGF ... However,
EGF dose-dependently
inhibited prolactin release from the stromal cells that were in the process of decidualization by co-stimulation with 8-Br-cAMP and EGF, though there was no significant change in viable cell numbers of the 8-Br-cAMP stimulated decidualizing cells
Wu et al., Zhongguo Yi Xue Ke Xue Yuan Xue Bao 1999
:
Detect the
effects of exogenous 17 beta-estradiol ( E2 ),
epidermal growth factor (EGF) , and transforming growth factor beta 1 ( TGF beta 1 ) on the proliferation and
prolactin (PRL) gene expression in primary serum-free cultured anterior pituitary cells in vitro
Romano et al., J Biol Chem 2003
:
Interestingly, Ras would be involved as a negative regulator of VIP/PACAP induced PRL gene activation, in contrast to its stimulatory role in the
regulation of the
PRL promoter by TRH and
EGF
Manfroid et al., Mol Cell Endocrinol 2005
(Breast Neoplasms) :
EGF stimulates Pit-1 independent transcription of the human
prolactin pituitary promoter in human breast cancer SK-BR-3 cells through its proximal AP-1 response element
Spuch et al., Endocrine 2006
:
EGF also
increases PRL levels in vitro ... We have found that FGF-2 ( 2 x 10 ( -11 ) M ) prevents the
EGF induced dose dependent increase in
PRL levels in control cells, and reversed the EGF stimulating effects in cells from E2-treated rats ... Both FGF-2 ( 2 x 10 ( -11 ) M ) and
EGF ( 6.6 x 10 ( -9 ) M ) significantly
increase ( > 30 % and > 120 %, respectively ) the
PRL levels in response to TRH ( 10 ( -6 ), 10 ( -5 ) M )
Huang et al., Oncogene 2006
(Breast Neoplasms) :
Furthermore, PRL synergized with EGF in activating SHC and ERK and transactivating a luciferase reporter driven by c-fos gene enhancer elements, suggesting that
PRL allowed markedly enhanced
EGF signaling ... Our data suggest that
PRL synergistically
augments EGF signaling in T47D breast cancer cells at least in part by lessening EGF induced EGFR downregulation and that this effect requires PRL induced ERK activity and threonine phosphorylation of EGFR
Spuch et al., Endocrine 2007
:
In addition, FGF-2 and
EGF , two important growth factors present in the pituitary,
reduced the PrRP induced
prolactin increase in vitro
Vlotides et al., Cancer Res 2008
(Pituitary Neoplasms...) :
EGF ( 5 nmol/L ) selectively enhanced baseline ( approximately 4-fold ) and serum
induced ( > 6-fold
) prolactin (PRL) mRNA levels, whereas gefitinib, an EGFR antagonist, suppressed serum induced cell proliferation and Pttg1 expression, blocked PRL gene expression, and reversed EGF mediated somatotroph-lactotroph phenotype switching
Chen et al., Am J Physiol Endocrinol Metab 2009
:
Using rat GH3 lactotrophs, we found that both E2 and
EGF independently
stimulated proliferation and
PRL gene expression
Tadokoro et al., Nihon Naibunpi Gakkai Zasshi 1990
:
[ The
effect of
epidermal growth factor (EGF) with estradiol and progesterone on
prolactin secretion from cultured human decidual tissues of early pregnancy ] ... We investigated the
effect of
epidermal growth factor (EGF) with estradiol and progesterone on
prolactin secretion by the decidual tissues from the early pregnant endometrium ... The
effect of high concentration
EGF ( 50 ng/ml ) on the
prolactin secretion appeared similar to the isolated decidual tissues
Reaves et al., Mol Endocrinol 1990
(Pituitary Neoplasms) :
Treatment with the combined hormones stimulates PRL synthesis approximately 6-fold, but this effect is not sufficient to increase PRL storage, because
epidermal growth factor alone
increases PRL synthesis to the same extent without affecting storage
Jackson et al., Mol Cell Endocrinol 1990
:
Furthermore, the acute stimulatory
effects of
EGF and TPA on expression of ( -187 )
PRL-CAT were additive
Ramsdell et al., J Cell Physiol 1989
:
Thyrotropin releasing hormone (TRH) and
epidermal growth factor both
enhance prolactin synthesis and substrate adhesion ( a morphological change called stretching ) of GH4 rat pituitary cells
Prysor-Jones et al., J Endocrinol 1987
(Pituitary Neoplasms) :
Prolactin secretion by a human pituitary tumour cell line produced in our laboratory was
stimulated by TRH, vasoactive intestinal peptide (VIP) and
epithelial growth factor (EGF)
Ramsdell et al., Endocrinology 1985
:
Thyrotropin releasing hormone and
epidermal growth factor stimulate
prolactin synthesis by a pathway ( s ) that differs from that used by phorbol esters : dissociation of actions by calcium dependency and additivity ... TRH stimulated PRL production was constant between 10 and 660 microM [ Ca2+ ] e, whereas
EGF stimulated
PRL production to a similar extent as TRH at 10 microM [Ca2+ ] e, but continued to enhance production with increasing [Ca2+ ] e. TRH elevated [Ca2+ ] i acutely, and at [Ca2+ ] e greater than 100 microM caused both a burst and a plateau phase in elevated [Ca2+ ] i ... We conclude that TPA stimulates PRL production by a highly calcium dependent pathway and that TRH and
EGF stimulate
PRL production by a different pathway ( s ) requiring lower [ Ca2+ ] i
Yajima et al., J Cell Physiol 1984
(Pituitary Neoplasms) :
The
effects of
epidermal growth factor on cell proliferation and
prolactin production by GH3 rat pituitary cells ...
EGF also
stimulated prolactin (PRL) production by culture in the presence or absence of serum
Brennessel et al., Mol Cell Biochem 1984
(Pituitary Neoplasms) :
TRH does not have any potentiating effect on
EGF induced stimulation of PRL release or
PRL synthesis in the hybrid strain
Ikeda et al., Endocrinology 1984
:
Secretion of
PRL or TSH was not
affected by
EGF at concentrations up to 50 ng/ml
Murdoch et al., Nature 1982
:
We have investigated the
effects of
EGF on
prolactin synthesis in the GH4 rat pituitary cell line to gain further insight into the mechanism of EGF 's actions on cellular functions
Meza et al., J Gen Physiol 1994
:
In rat pituitary GH3 cells,
epidermal growth factor (EGF) and insulin
stimulate prolactin production, whereas glucocorticoids exert the opposite effect
Fenton et al., Exp Cell Res 1994
:
Furthermore,
PRL reduced the percentage of cells in S-phase to IGF-1- and CT-induced levels in the presence of
EGF
Berwaer et al., Mol Cell Endocrinol 1993
(Pituitary Neoplasms) :
Thyrotropin releasing hormone and
epidermal growth factor induce human
prolactin expression via identical multiple cis elements
Aanestad et al., Acta Endocrinol (Copenh) 1993
(Pituitary Neoplasms) :
Epidermal growth factor (EGF) stimulated the
prolactin (PRL) synthesis and release from the GH4C1 cells in a dose dependent manner ...
EGF stimulated the release of
PRL from cell perfusion columns after a lag period of about 30 s ... The
EGF stimulated
PRL release was additive to the secretion evoked by thyrotropin releasing hormone (TRH) and vasoactive intestinal peptide (VIP)
Zhang et al., Proc Soc Exp Biol Med 1993
(Pituitary Neoplasms) :
Effects of estrogen and
epidermal growth factor on
prolactin and Pit-1 mRNA in GH3 cells ... The
effects of
epidermal growth factor (EGF) and 17 beta-estradiol ( E2 ) on the expression of
prolactin (PRL) , the transcription factor Pit-1/GHF-1 ( Pit-1 ), and on dopamine D2 receptor mRNA in GH3 cells were analyzed by immunocytochemistry, in situ hybridization, and Northern analysis in a defined serum-free cell culture medium ... Both
EGF and E2
stimulated PRL mRNA and
PRL secretion, although the effects of EGF were more rapid than those of E2
Altschuler et al., Neuroendocrinology 1993
:
The present work studied the
effects of
epidermal growth factor (EGF) on the release of thyrotropin ( TSH ) and
prolactin (PRL) from perifused pituitary glands of 200-gram male Wistar rats ... However, TRH, but not
EGF ,
stimulated PRL secretion ( p < 0.01 )
Nowak et al., J Clin Endocrinol Metab 1993
(Leiomyoma...) :
17 beta-Estradiol, progesterone, TRH, insulin-like growth factor-I,
epidermal growth factor , and the GnRH agonist leuprolide did not
affect PRL secretion by these SMC
Johnson et al., J Biol Chem 1996
:
As expected we found that
EGF stimulated an association of EGF-R with Grb2,
PRL , however, blocked this association
CastaƱo et al., Mol Endocrinol 1996
:
Levels of PRL gene transcription were quantified by photonic imaging in the same cells before and after 24 h of culture in the presence or absence of the dopamine agonist bromocryptine or
epidermal growth factor , two well known
regulators of
PRL gene transcription
Billis et al., Biochim Biophys Acta 1997
(Pituitary Neoplasms) :
Herbimycin A inhibited the ability of bFGF,
EGF and TRH to
stimulate PRL gene expression in GH3 cells