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SMAD2 — SP1
Pathways - manually collected, often from reviews:
-
OpenBEL Selventa BEL large corpus:
SP1
→
SMAD2
(increases, SMAD2 Activity)
Feng et al., EMBO J 2000
Evidence: In the nucleoprotein complex, Smad2 interacted through its C-domain with Sp1 and enhanced the DNA binding and transcriptional activity of Sp1
-
OpenBEL Selventa BEL large corpus:
SP1
→
Complex of SMAD2-SP1
(directlyIncreases, SMAD2/SP1 Activity)
Feng et al., EMBO J 2000
Evidence: In the nucleoprotein complex, Smad2 interacted through its C-domain with Sp1 and enhanced the DNA binding and transcriptional activity of Sp1
-
FastForward regulation:
SP1
→
SMAD2
(transcriptional regulation, unknown)
Evidence: DNABINDING
-
KEGG TGF-beta signaling pathway:
Complex of SMAD2-SMAD3-SMAD4
→
Complex of CREBBP-EP300-SP1
(protein-protein, activation)
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NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
AP1 complex (FOS-JUN)
→
SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1)
(transcription, activates)
Brodin et al., J Biol Chem 2000
Evidence: mutant phenotype, reporter gene, physical interaction, other species
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
SMAD2-3/SMAD4/SP1/MIZ-1 complex (SMAD2_SMAD3-SMAD4-SP1-ZBTB17)
→
SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1)
(modification, collaborate)
Seoane et al., Nat Cell Biol 2001
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
SMAD2-3/SMAD4/SP1/MIZ-1 complex (SMAD2_SMAD3-SMAD4-SP1-ZBTB17)
→
MIZ-1 (ZBTB17)
(modification, collaborate)
Seoane et al., Nat Cell Biol 2001
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1)
→
MIZ-1 (ZBTB17)
(modification, collaborate)
Seoane et al., Nat Cell Biol 2001
Evidence: mutant phenotype, physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
MYC/MIZ-1 complex (MYC-ZBTB17)
→
SMAD2-3/SMAD4/SP1/MIZ-1 complex (SMAD2_SMAD3-SMAD4-SP1-ZBTB17)
(transcription, inhibits)
Feng et al., EMBO J 2000, Seoane et al., Nat Cell Biol 2001, Matsuura et al., Nature 2004
Evidence: mutant phenotype, reporter gene, physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
SMAD3/SMAD4 complex (SMAD3-SMAD4)
→
SMAD2-3/SMAD4/SP1/MIZ-1 complex (SMAD2_SMAD3-SMAD4-SP1-ZBTB17)
(transcription, inhibits)
Feng et al., EMBO J 2000, Seoane et al., Nat Cell Biol 2001, Matsuura et al., Nature 2004
Evidence: mutant phenotype, reporter gene, physical interaction
-
NCI Pathway Database Validated targets of C-MYC transcriptional repression:
SMAD2-3/SMAD4/MYC/Max/MIZ-1 complex (MYC-MAX-ZBTB17-SMAD2_SMAD3-SMAD4)
→
SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1)
(transcription, inhibits)
Staller et al., Nat Cell Biol 2001, Seoane et al., Nat Cell Biol 2001, Feng et al., Mol Cell 2002
Evidence: mutant phenotype, reporter gene, physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
SP1 (SP1)
→
SMAD2-3/SMAD4 complex (SMAD2_SMAD3-SMAD4)
(modification, collaborate)
Feng et al., EMBO J 2000
Evidence: physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
SP1 (SP1)
→
SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1)
(modification, collaborate)
Feng et al., EMBO J 2000
Evidence: physical interaction
-
NCI Pathway Database Regulation of nuclear SMAD2/3 signaling:
SMAD2-3/SMAD4 complex (SMAD2_SMAD3-SMAD4)
→
SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1)
(modification, collaborate)
Feng et al., EMBO J 2000
Evidence: physical interaction
-
Reactome Reaction:
SP1
→
SMAD2
(reaction)
Feng et al., EMBO J 2000
-
Reactome Reaction:
SP1
→
SMAD2
(indirect_complex)
Feng et al., EMBO J 2000
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Biogrid Interaction:
SMAD2
—
SP1
(physical association, affinity chromatography technology)
Poncelet et al., J Biol Chem 2001*
-
IRef Biogrid Interaction:
SMAD2
—
SP1
(direct interaction, two hybrid)
Pardali et al., J Biol Chem 2000*
-
IRef Biogrid Interaction:
SMAD2
—
SP1
(direct interaction, pull down)
Pardali et al., J Biol Chem 2000*
-
IRef Biogrid Interaction:
SMAD2
—
SP1
(physical association, affinity chromatography technology)
Pardali et al., J Biol Chem 2000*
-
IRef Biogrid Interaction:
SMAD2
—
SP1
(physical association, affinity chromatography technology)
Barrasa et al., Biochim Biophys Acta 2012*
-
IRef Hprd Interaction:
SP1
—
SMAD2
(in vivo)
Poncelet et al., J Biol Chem 2001*
-
IRef Ophid Interaction:
SMAD2
—
SP1
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
Feng et al., EMBO J 2000
:
In the nucleoprotein complex,
Smad2 interacted through its C-domain with Sp1 and
enhanced the DNA binding and transcriptional activity of
Sp1
Jungert et al., Carcinogenesis 2006
(Pancreatic Neoplasms) :
Moreover, inhibition of
Sp1-DNA binding or transfection of Sp1-specific siRNA prevents TGFbeta induced Smad7 expression and consequently
enhances Smad signaling in pancreatic cancer cells, as indicated by increased receptor mediated phosphorylation of Smad3
Wang et al., Differentiation 2011
:
The increase of
Sp1 , but not
Smad 2/3 activation was almost completely blocked by the addition of TSA