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EPHB2 — NGF

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Rui et al., J Biol Chem 1999 : Overexpression of either wild type or mutant SH2-Bbeta ( R555E ) did not alter tyrosyl phosphorylation of TrkA, Shc, or phospholipase Cgamma in response to NGF or NGF induced activation of ERK1/2 , suggesting that SH2-Bbeta may initiate a previously unknown pathway ( s ) that is essential for NGF induced neurite outgrowth
Zhong et al., J Neurochem 1999 : Nerve growth factor (NGF) caused similar levels of ERK activation in all subclones
Masuelli et al., Cell Growth Differ 1999 : The activation of Erk kinase activity in response to NGF was sustained longer in the Rsu-1 transfectants compared with the vector control cells
Kamakura et al., J Biol Chem 1999 : Here we have found that ERK5 is strongly activated by epidermal growth factor and nerve growth factor , whose receptors are tyrosine kinases
Zapf-Colby et al., Oncogene 1999 : NGF and IGF-I induce extracellular-signal regulated kinase 1 ( ERK1 ) and ERK2 activation, but NGF is able to stimulate a higher and more sustained activation of these enzymes compared with IGF-I
Zhang et al., Neurobiol Aging 1999 (Alzheimer Disease...) : Oxidative stress differentially modulates phosphorylation of ERK , p38 and CREB induced by NGF or EGF in PC12 cells
You et al., Invest Ophthalmol Vis Sci 2000 : In epithelium, predominantly ERK 1 was activated by NGF , GDNF, and BDNF ... In stromal cells NGF and GDNF stimulated phosphorylation of ERK 1 and JNK 1 ... GDNF and NGF stimulate corneal epithelial proliferation, and the effect of the latter might be mediated by activation of ERK 1
Olsson et al., Exp Cell Res 2000 (Neuroblastoma) : Both TPA and NGF induced a sustained activation and nuclear accumulation of ERK that was accompanied by transactivation of a serum response element ( SRE ) -driven reporter and of the c-fos gene
Colucci-D'Amato et al., J Biol Chem 2000 : Despite the presence of MKP-3, ERK activity can be further stimulated by NGF , but it fails to translocate into the nucleus and consequently to induce immediate-early gene transcription
Fletcher et al., J Cell Biol 2000 : Despite prolonged Akt and ERK signaling induced by NGF after BAF treatment has prevented death, the neurons fail to increase protein synthesis, recover ATP levels, or grow
Arslan et al., Neurosci Lett 2000 : These results indicate that adenosine A(2A) receptor induced increases in cyclic AMP can stimulate ERK1/2 phosphorylation per se, inhibit the initial and enhance the late NGF induced activation of ERK1/2
Burry et al., J Neurosci Res 2001 (MAP Kinase Signaling System) : However, NGF stimulated phosphorylation of ERK , p38, and Akt in PC12-N09rasWT cells is similar in duration to that in PC12-N09 cells, indicating that the p21(ras) signaling through ERK, p38, and Akt was not involved in the restoration of normal neurite elongation in PC12-N09 cells
Williams et al., Neurotoxicology 2000 : Lead also enhanced NGF induced ERK phosphorylation and activation, but lead alone did not stimulate ERK ... The MAP kinase kinase ( MEK ) inhibitor, PD98059, significantly decreased the effect of lead on NGF induced neurite outgrowth and ERK activation
Harada et al., Nat Cell Biol 2001 : In PC12 cells, sustained activation of ERK induced by nerve-growth factor (NGF) is essential for neuronal differentiation
Barouch et al., J Leukoc Biol 2001 (MAP Kinase Signaling System) : NGF induced phosphorylation and activation of extracellular signal regulated kinase, Erk1/Erk2 and c-Jun amino-terminal kinase, whereas it did not induce phosphorylation of p38 mitogen activated protein kinase
Kinkl et al., J Biol Chem 2001 : Furthermore U0126 treatment entirely blocked nerve growth factor induced ERK1/2 activation in MGC, as well as FGF2 induced ERK1/2 activation in cerebral glial cells
Nakayama et al., J Neurochem 2001 (MAP Kinase Signaling System) : The maximal level of nicotine induced ERK phosphorylation was lower than that of the membrane depolarization induced and, to a great extent, the nerve growth factor (NGF) induced ERK phosphorylation
Zentrich et al., J Biol Chem 2002 : Truncation analysis and electromobility shift assays established the requirement for a cAMP-response element/activating transcription factor-like site in the NFLC promoter that minimally interacts with constitutively expressed cAMP-response element binding protein and JunD as well as c-Jun which is induced by NGF in an ERK dependent manner
Jiang et al., J Mol Neurosci 2001 : A double cysteine trkA mutant exhibiting reduced NGF binding and delayed Erk signaling ... NGF induced Erk phosphorylation was rapid in WT11 and C423T cells, but delayed in C436A and C423T/C436A cells ... However, basic fibroblast growth factor (bFGF) induced rapid phosphorylation of Erk1/2, and [ 3H ] thymidine incorporation in NIH3T3, WT11, single mutant ( SM ), and double mutant ( DM ) cells, suggesting that the impaired NGF induced Erk phosphorylation and thymidine incorporation observed in DM cells are due to the double-cysteine mutations in the trkA receptor
Dagø et al., Neurochem Res 2002 : NS-417 also enhanced ERK activation induced by 10 minutes stimulation with NGF , bFGF or EGF in PC12 cells
Watts et al., American journal of physiology. Renal physiology 2002 : The selective MAP kinase kinase (MEK1/2) inhibitors U0126 and PD-98059 abolished the NGF induced ERK activation and largely eliminated ( > or = 60 % ) the effects of NGF to inhibit basolateral Na ( + ) /H ( + ) exchange activity and transepithelial HCO absorption in perfused MTALs
Agell et al., Cell Signal 2002 (Calcium Signaling...) : CaM binding proteins (CaMBPs) as Ras-GRF and CaM dependent protein kinase IV (CaMKIV) positively modulate ERK1/2 activation induced by either NGF or membrane depolarisation in neurones
Dagø et al., J Neurochem 2002 (Disease Models, Animal...) : At the molecular level, NS 1231 enhanced NGF induced signalling events, such as TrkA phosphorylation at the Shc binding site Tyr490 as well as ERK activation in PC12 cells
Cantarella et al., FASEB J 2002 : Endogenous NGF was responsible for the basal levels of trkA ( NGFR ) and ERK1/2 phosphorylation observed in untreated HUVEC cultures
Chen et al., Dev Biol 2002 : Mutant ( but not wt ) SHP-2 expression also inhibited NGF stimulated ERK activation
Horgan et al., Exp Cell Res 2003 : In PC12 cells, NGF and EGF induce a rapid translocation of GFP-Erk that requires Ras and Mek
Takeuchi et al., Biochem Pharmacol 2003 : Following prolonged incubation in RS when ERK activity subsided to near basal levels, NGF restored ERK1/ERK2 activity to the elevated level initially observed in RS
Obata et al., J Neurosci 2003 (Inflammation) : The intrathecal application of nerve growth factor (NGF) induced an increase in the number of p-ERK-and BDNF labeled cells, mainly small neurons, and the application of anti-NGF induced an increase in p-ERK and BDNF in some medium-to-large-diameter DRG neurons
Edwards et al., Brain Res 2003 : The I ( 1 ) -imidazoline receptor in PC12 pheochromocytoma cells reverses NGF induced ERK activation and induces MKP-2 phosphatase
Haycock et al., Proc Natl Acad Sci U S A 1992 : Pretreatment of the cells with genistein ( a protein-tyrosine kinase inhibitor ) decreased the bradykinin- but not the NGF induced changes in both TH-Ser31 phosphorylation and ERK activity
Steinle et al., Auton Neurosci 2003 : NGF stimulation resulted in activation of ERK1/2 , Akt, and Src in choroidal endothelial cells, while little phosphorylation was noted following NGF treatment in retinal endothelial cells
Loeb et al., Neuron 1992 : As detected by coimmunoprecipitation from PC12 cells, NGF induces rapid association between ERK1 ( a growth factor activated serine/threonine protein kinase ) and gp140prototrk NGF receptors
Tsuruda et al., FEBS Lett 2004 (MAP Kinase Signaling System...) : Activation of TrkA and extracellular signal regulated kinase ( ERK ) by nerve growth factor (NGF) was inhibited by pretreatment with PP2, an inhibitor of Src family kinases ... Moreover, NGF induced phosphorylation of TrkA and ERK was also attenuated by the transfection with a dominant negative src construct
Kawa et al., Genes Cells 2004 : Introduction of a kinase-defective mutant of BREK into PC12 cells enhanced both ERK phosphorylation and neurite outgrowth in response to NGF , suggesting that BREK is a negative regulator of NGF induced neuronal differentiation
Parran et al., Brain Res Dev Brain Res 2004 : NGF induced phosphorylation of ERK1/2 in PC12 cells was time dependent ... However, NGF stimulated ERK1/2 phosphorylation was not altered after 5 min of exposure to CH3Hg
Bumeister et al., Curr Biol 2004 (MAP Kinase Signaling System) : For example, the mitogen EGF induces a transient ERK activation, whereas the neurotrophin NGF induces prolonged ERK activation
Ahamed et al., J Immunol 2004 : Surprisingly, C3a significantly enhanced NGF induced NFAT activation, ERK phosphorylation, and MIP-1beta production
Obata et al., Neuroscience 2004 (Pain...) : Therefore, we suggest that after inflammation near the cell body, NGF synthesized within the nerve root and DRG induces BDNF expression through trkA receptors and intracellular ERK-MAPK
Zhuang et al., J Neurosci 2004 (Hyperalgesia...) : ERK activation by capsaicin and NGF was also blocked by PI3K inhibitors
Dey et al., Exp Cell Res 2005 : CSK overexpression caused a profound inhibition of NGF induced activation of FYN, YES, RAS, and ERK and inhibited neurite outgrowth, NGF stimulated integrin directed migration and blocked the NGF induced conversion of GDP-RAC to its GTP bound active state
Yang et al., Ann N Y Acad Sci 2005 (MAP Kinase Signaling System) : Antioxidant N-acetylcysteine blocks nerve growth factor induced H2O2/ERK signaling in PC12 cells ... We found that NGF increased intracellular H2O2 concentration and activated ERK but failed to affect intracellular superoxide level ... These findings demonstrate that NAC blocks the NGF induced H2O2/ERK signaling in PC12 cells
Pizzio et al., Eur J Neurosci 2005 : NGF activates c-FOS and ERK1/2-MAPK in the suprachiasmatic nuclei, the site of a circadian clock in mammals, when administered during the subjective night but not during the day ... The effect of NGF on ERK1/2 activation was not inhibited by the administration of MK-801, a glutamate/NMDA receptor antagonist
Shi et al., J Biol Chem 2005 (MAP Kinase Signaling System) : Accordingly, selective down-regulation of Rin in PC6 cells suppressed neurotrophin elicited activation of b-Raf and p38, without obvious effects on NGF induced ERK activation
Robbins et al., Mol Biol Cell 1992 : The phosphorylation of extracellular signal regulated kinases 1 and 2 ( ERK1 and ERK2 ) in response to insulin in Rat 1 HIRc B cells and in response to nerve growth factor (NGF) in PC12 cells has been examined ... NGF stimulates tyrosine phosphorylation of ERK1 more rapidly than threonine phosphorylation
Park et al., Biochem Biophys Res Commun 2006 : In the presence of ERK inhibitor, PD98059, the generation of beta-NGF was diminished in a dose dependent manner consistent with the inhibiting effect on neuronal differentiation
Hongo et al., J Cell Biochem 2006 : Ndrg4 enhances NGF induced ERK activation uncoupled with Elk-1 activation ... PC12 cell lines stably expressing increased levels of Ndrg4 protein display enhanced NGF induced phosphorylation of MEK and ERK
Lee et al., J Neurochem 2006 : Levels of TrkA receptor phosphorylation and downstream ERK activation induced by NGF were not influenced by GnT-VB expression
El Zein et al., Cell Signal 2007 : Finally, NGF dependent ERK activation and H2O2 production is pertussis toxin sensitive
Williams et al., Psychopharmacologia 2006 : ERK mediated NGF signaling in the rat septo-hippocampal pathway diminishes with age
Ziegler et al., Ann N Y Acad Sci 2006 : In the present study, we examined the influence of DHEA on nerve growth factor (NGF) mediated survival in serum deprived PC12 cells and analyzed the influence of DHEA on NGF induced ERK1/2 mitogen activated protein ( MAP ) kinase activation by enzyme linked immunosorbent assay ( ELISA )
Noordman et al., Journal of molecular signaling 2006 : We studied the physiological role of kinase interaction motif ( KIM ) -containing protein tyrosine phosphatases (PTPs) in the control of EGF- and NGF induced ERK activity in neuroendocrine PC12 cells ... Protein knock-down of PCPTP1, or fourfold overexpression of its mouse orthologue, PTPBR7, left EGF- and NGF induced ERK1/2 activity in PC12 cells unaltered
Arien-Zakay et al., J Mol Neurosci 2007 (Neuroblastoma) : Combined CM and NGF treatment induced constitutive activation of the mitogen activated protein kinases ERK2 ( 36-fold vs control ), p38alpha ( nine-fold vs control ) and p38beta ( 23-fold vs control ), most likely related to survival and/or differentiation
Ziegler et al., Endocrinology 2008 (Adrenal Gland Neoplasms...) : We demonstrate that NGF induced ERK1/2 phosphorylation was reduced by DHEA
Samoylenko et al., Cell Signal 2008 : Thrombopoietin inhibits nerve growth factor induced neuronal differentiation and ERK signalling ... Using phospho-kinase arrays and Western blot we found downregulation of the NGF stimulated phosphorylation of the extracellular signal regulated kinase p42ERK by TPO with no effect on phosphorylation of Akt or stress kinases ... Our data indicate that TPO via activation of its receptor bound JAK2 delays the NGF dependent acquisition of neuronal phenotype and decreases neuronal survival by suppressing NGF induced ERK activity
Takei et al., Mol Biol Cell 2008 (Neuroblastoma...) : It increases Erk activation by NGF , and this increased activation induces differentiation of neuroblastoma cell lines
Kusunoki et al., Neurosci Lett 2008 : Intriguingly, we also discovered that decreased phosphorylation of ERK1/2 was induced by p-nonylphenol in the presence of NGF , whereas p-nonylphenol alone did not induce phosphorylation of ERK1/2
Yang et al., Neurosci Lett 2008 (Brain Ischemia...) : NGF/VEGF significantly inhibited the expression of ERK1 and caspase-3
de Melo Reis et al., Brain Res 2008 : Murine Müller glial conditioned medium ( MMG ) was concentrated and at 1 : 1 dilution supported 100 % survival of chick or rat sympathetic neurons after 48 h compared to < 5 % in controls. Partial purification of the MMG using centriprep concentrators showed that trophic activity is from molecules above 10 kDa. MMG stimulated AKT, ERK and pStat3 in sympathetic neurons. Sympathetic or DRG neuronal survival induced by MMG was blocked by anti-human NGF , but not by anti-human CNTF ( sympathetic ) or by anti-BDNF ( DRGs ) neutralizing antibodies. MMG also induced neurite outgrowth in P4 mice retinal explants and on isolated RGC. RGCs plated on top of Müller glia cells had a much better survival rate ( > 80 %, 96 h ) compared to laminin+poly-L-lysine substrates
Barhwal et al., J Neurosci Res 2008 (MAP Kinase Signaling System) : Hypoxia induced deactivation of NGF mediated ERK1/2 signaling in hippocampal cells : neuroprotection by acetyl-L-carnitine
Lawrence et al., Proc Natl Acad Sci U S A 2008 (MAP Kinase Signaling System) : We also find that glucose and NGF stimulate the binding of ERK1/2 to the insulin gene and other promoters
Yamada et al., J Pharmacol Sci 2008 : We describe here our finding that the NGF induced phosphorylation of both ERK and Akt are accelerated by MCC-257
Krug et al., Mol Cell Endocrinol 2009 : On a molecular level, DHEA-S diminished NGF induced ERK1/2 phosphorylation ... Interference of DHEA-S with NGF stimulated ERK1/2 activation might be involved in this effect
Yasuda et al., Cell cycle (Georgetown, Tex.) 2008 : In classical experiments, PC12 cells proliferated after transient ERK activation by epidermal growth factor, but terminally differentiated after more sustained ERK activation by nerve growth factor
Terasawa et al., FEBS J 2009 : Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells
Julio-Pieper et al., J Clin Endocrinol Metab 2009 (MAP Kinase Signaling System) : NGF induces MAPK-ERK2 phosphorylation, and blockade of this signaling pathway prevents the NGF induced increase in VEGF production
Contrepas et al., Am J Physiol Regul Integr Comp Physiol 2009 (Epilepsy...) : In contrast, Delta4- ( P ) RR remained cytosolic and inhibited NGF induced neuronal differentiation and ERK1/2 activation
Obara et al., J Biol Chem 2009 : Nerve growth factor (NGF) induced phosphorylation of both ERK5 and ERK1/2, whereas the cAMP analog dibutyryl cAMP ( Bt ( 2 ) cAMP ) caused only ERK1/2 phosphorylation ... Both NGF and Bt ( 2 ) cAMP increased tyrosine hydroxylase gene promoter activity in an ERK1/2 dependent manner but was ERK5 independent
Chung et al., J Biol Chem 2010 (Cystitis...) : Suppression of endogenous NGF level with neutralizing NGF antibody significantly blocked the increased activity of Akt, JNK, and ERK1/2 in the inflamed bladder during cystitis
Feng et al., J Neurosci Res 2010 : In addition, in the NRAGE suppressed cells, NGF induced ERK activation is increased and this activation is MEK dependent ... Conversely, NRAGE overexpression significantly represses NGF induced ERK activation
Niewiadomska et al., Behav Brain Res 2011 (Alzheimer Disease) : NGF released from target cells activates TrkA on axon terminals and triggers activation of PI3K/Akt, MEK/ERK , and PLC? ( phospholipase C ) signaling pathways
Lu et al., Brain Pathol 2010 (Disease Models, Animal) : On one hand, NGF/TrkA induced activation of Akt and ERK1/2 , which led to neuronal survival ; on the other hand, NGF/TrkA mediated CaMKII and CREB phosphorylation and increased PSD95 expression, which improved cognitive performance
Cassano et al., J Biol Chem 2010 (MAP Kinase Signaling System) : Long term activation of ERK1/2 by NGF requires SOD activation, low levels of hydrogen peroxide, and the integrity of the microtubular cytoskeleton
Rao et al., Mol Cancer Ther 2010 (Leukemia, Myeloid, Acute) : Exposure to 17-DMAG inhibited NGF induced p-TrkA, p-AKT, and p-ERK1/2 levels, as well as induced apoptosis of K562, 32D cells with ectopic expression of wild-type TrkA or the constitutively active mutant Delta TrkA, and of primary myeloid leukemia cells
Mullenbrock et al., J Biol Chem 2011 (MAP Kinase Signaling System) : Sustained ERK activity induced by NGF , as compared with transient activity induced by EGF, is critical to the differentiation of these cells ... To characterize the transcriptional program activated preferentially by NGF, we compared global gene expression profiles between cells treated with NGF and EGF for 2-4 h, when sustained ERK signaling in response to NGF is most distinct from the transient signal elicited by EGF
Areshkov et al., International journal of biological sciences 2012 (MAP Kinase Signaling System) : Sustained activation of ERK1/2 by nerve growth factor (NGF) is associated with translocation of ERKs to the nucleus of PC12 cells and precedes their differentiation into sympathetic-like neurons whereas transient activation by epidermal growth factor (EGF) leads to cell proliferation
Matsuoka et al., Neuroscience 2012 (MAP Kinase Signaling System...) : AZD6244, a potent selective inhibitor of ERK1/2 activation, blocked NGF induction of BDNF mRNA in vitro suggesting that NGF induction of BDNF is mediated by ERK1/2
Alsina et al., PloS one 2012 (MAP Kinase Signaling System) : At molecular level, our findings indicate that ectopic expression of a mutated form of Spry4 ( Y53A ), in which a conserved tyrosine residue was replaced, fail to block both TrkA mediated Erk/MAPK activation and neurite outgrowth induced by NGF , suggesting that an intact tyrosine 53 site is required for the inhibitory effect of Spry4 on NGF signaling
Ettinger et al., Cell Signal 2012 (Ischemia) : To achieve this goal we sought to investigate NGF induced ERK1/2 phosphorylation and its role in the C2C12 skeletal muscle myoblasts and myotubes ... Both proNGF and ßNGF induced ERK1/2 phosphorylation, a process blocked by ( a ) the specific MEK inhibitor, PD98059 ; ( b ) VLO5, a MLD-disintegrin with relative selectivity towards a9ß1 integrin ; and ( c ) p75 ( NTR ) antagonists Thx-B and LM-24, but not the inactive control molecule backbone Thx
Ochodnický et al., Pharmacol Res 2013 (Urinary Bladder, Overactive) : Bradykinin increased NGF mRNA expression and accelerated urothelial NGF release to 127±5 % in a protein kinase C- and ERK1/2 dependent manner
Aletta et al., Neuroreport 1994 : Differential effect of NGF and EGF on ERK in neuronally differentiated PC12 cells
Frödin et al., J Biol Chem 1994 : Finally, cAMP or PACAP38 increased by 3-fold nerve growth factor stimulated neurite formation in PC12 cells, which may be correlated with the potentiating effect of these agents on nerve growth factor stimulated ERK1 activity
Stephens et al., Neuron 1994 : However, PC12nnr5 cells expressing Trk with mutations at both sites failed to stably extend neurites and efficiently induce Erk1 activity and tyrosine phosphorylation in response to NGF
Nguyen et al., J Biol Chem 1993 : Thus, NGF induced a nuclear translocation of ERK1 , while epidermal growth factor was without noticeable effect on ERK1 localization
Volonté et al., J Biol Chem 1993 : In cultured rat pheochromocytoma PC12 cells, ERK1 and ERK2 are activated by nerve growth factor (NGF) , which also induces rapid association between ERK1 and the high affinity gp140prototrk tyrosine kinase NGF receptor ... NGF did not modulate the total amount of ERK proteins coimmunoprecipitated with p75 but did markedly stimulate the level of p75 associated ERK catalytic activity
Wu et al., J Biol Chem 1996 : Mechanistically, NGF and bFGF induce a sustained phosphorylation and activation of ERK1 and ERK2 in both cells, while IL-6 produces only a transient and weak tyrosine phosphorylation
Wu et al., J Biol Chem 1996 : Importantly, IL-6 does not enhance ERK phosphorylation in the presence of either NGF or EGF
Calleja et al., Endocrinology 1997 : Thus, in NIH3T3 fibroblasts expressing NGF receptors ( NIH3T3/trk cells ) we found that cAMP potentiates NGF stimulated ERK1 and MEK1 activities, whereas in NIH3T3 fibroblasts expressing insulin receptors ( NIH3T3/IR cells ) we saw no effect of cAMP on the activation of insulin stimulated ERK1 and MEK1
Jiang et al., J Cell Biochem 1997 : NGF induced Erk stimulation for 60 min was assessed using myelin basic protein as a substrate
Yao et al., J Biol Chem 1998 : Induction of neuronal differentiation of the rat pheochromocytoma cell line, PC12 cells, by nerve growth factor (NGF) requires activation of the mitogen activated protein ( MAP ) kinase or extracellular signal regulated kinase ( ERK ) ... Here we show that maximal sustained activation of ERK1 by NGF requires PKA ... Inhibitors of PKA partially blocked activation of ERK1 by NGF but had no effect on activation of ERK1 by EGF ... We propose that PKA is an important contributor to the activation of ERK1 by NGF and is required for maximal induction of gene expression by NGF
Camps et al., FEBS Lett 1998 : Consistent with a role mediating inhibition of PC12 cell MAP kinases, NGF stimulated ERK2 activation was suppressed considerably following pretreatment with fibroblast growth factor and 9-cis-retinal, two additional differentiation factors found to induce powerfully MKP-3 expression
Yaka et al., J Cell Biochem 1998 : NGF induces transient but not sustained activation of ERK in PC12 mutant cells incapable of differentiating
Powers et al., Cell Tissue Res 1999 : NGF induced phosphorylation of ras mediated mitogen activated protein kinases ( MAPK ) Erk1 and Erk2 is as prolonged in normal chromaffin cells as in PC12 cells, where NGF is neuritogenic