◀ Back to IRF6

IRF6 — VEGFA

Text-mined interactions from Literome

Matsushita et al., Infect Immun 1999 : We investigated whether vascular endothelial growth factor ( VEGF ) production by human pulp cells ( HPC ) is regulated by lipopolysaccharide (LPS) in relation to the pathogenesis of pulpitis ... Although HPC incubated with medium alone only marginally expressed VEGF mRNA and produced a low level of VEGF as detected by enzyme linked immunosorbent assay, the VEGF mRNA expression and VEGF production were markedly enhanced upon stimulation with LPS from Escherichia coli ... Prevotella intermedia LPS , phorbol 12-myristate 13-acetate, and interleukin-6 also induced VEGF mRNA expression in HPC ... However, the anti-CD14 monoclonal antibody MY4 inhibited VEGF induction upon stimulation with LPS in HPC cultures in the presence of 10 % FBS but not in the absence of FBS ... LPS augmented the VEGF production in HPC cultures in the presence of recombinant human soluble CD14 ( sCD14 ) ... To clarify the mechanisms of VEGF induction by LPS , we examined the possible activation of the transcription factor AP-1 in HPC stimulated with LPS, by a gel mobility shift assay ... The AP-1 inhibitor curcumin strongly inhibited LPS induced VEGF production in HPC cultures ... In addition, a protein synthesis inhibitor, cycloheximide, inhibited VEGF mRNA accumulation in response to LPS
Pérez-Ruiz et al., Hepatology 1999 (Ascites...) : These results indicate that cytokines and LPS markedly increase VEGF protein secretion and mRNA expression in macrophages of cirrhotic patients, and suggest that this substance could be an important mediator of the pronounced arterial vasodilation frequently occurring in SBP patients
Nagashima et al., Clin Exp Immunol 1999 (Arthritis, Rheumatoid...) : BUC significantly inhibited LPS induced VEGF production, while GST tended to inhibit the production of VEGF ... Reverse transcriptase-polymerase chain reaction ( RT-PCR ) analysis showed that BUC also inhibited LPS induced VEGF mRNA expression in RA synovial cells
Meng et al., Shock 1999 (Inflammation...) : LPS induced cardiac bFGF and VEGF at 6 and 12 h after treatment
Sugishita et al., Biochem Biophys Res Commun 2000 : To test the hypothesis that VEGF is one of the important mediators of SIRS, we examined effects of LPS on the VEGF expression and secretion in cultured neonatal rat ventricular myocytes ... Pharmacological inhibition of nucleic factor-kappaB or tyrosine kinases did not affect the LPS induced augmentation of VEGF mRNA expression, while these treatments markedly suppressed the up-regulation of inducible nitric oxide synthase (iNOS) expression by LPS
Itaya et al., Thromb Haemost 2001 : We have studied the production of VEGF by human macrophages in response to lipopolysaccharide (LPS) ... The LPS induced expression of VEGF was inhibited by cycloheximide pretreatment, which suggested that synthesis of certain factor ( s ) is required for the LPS activity ... These results suggest that the LPS induced VEGF expression depends on the p38 mediated expression of c-Jun, which constitutes the AP-1 complex and binds to the AP-1 site in the VEGF promoter ... Pretreatment of the cells with dexamethasone did not affect the LPS induced upregulation of VEGF mRNA but strongly inhibited VEGF protein production, and the involvement of posttranscriptional regulation on VEGF expression by dexamethasone was suggested ... We conclude that macrophages produce VEGF in response to the stimulation with LPS , which may be partly mediated by the p38 MAP kinase pathway
Mukutmoni et al., Prostaglandins Leukot Essent Fatty Acids 2001 : A decreasing trend was seen in LPS induced VEGF secretion from macrophages in vitro after docosahexaenoic acid or eicosapentaenoic acid incubation compared to arachidonic acid
Power et al., J Surg Res 2001 : The observed downregulation of constitutive and inducible VEGF receptor expression on ECs was not due to any directly cytotoxic effect of LPS and BLP on ECs, as measured by cell viability and apoptosis assays
Leibovich et al., Am J Pathol 2002 : VEGF expression is strongly up-regulated by LPS/NECA in macrophages from A ( 3 ) -/- but not A ( 2A ) -/- mice, confirming the role of adenosine A ( 2A ) receptors in this pathway
Kiriakidis et al., J Cell Sci 2003 (Inflammation...) : We observed that LPS induced production of VEGF in human macrophages was almost completely inhibited ( > 90 % ) following adenoviral transfer of IkappaBalpha ... Expression of IKK-2dn in macrophages decreased VEGF production in response to LPS or CD40L by approximately 50 %, suggesting that in addition to IKK-2, other kinases might be involved in NF-kappaB activation
Suthin et al., J Periodontal Res 2003 (Neovascularization, Pathologic...) : On the other hand, VEGF levels were not increased by LPS stimulation
Ramanathan et al., Experimental biology and medicine (Maywood, N.J.) 2003 (Anoxia) : However, the iNOS inhibitor aminoguanidine ( AG ) decreases the VEGF expression induced by LPS/IFN-gamma , indicating an important role for NO ... LPS/IFN-gamma treatment induces minimal VEGF protein expression in macrophages cultured at low cell densities ( < 0.25 x 10 ( 6 ) cells/cm ( 2 ) ) ; at higher cell densities ( > 0.25 x 10 ( 6 ) cells/cm ( 2 ) ) that lead to conditions of pericellular hypoxia, however, induction of VEGF expression was strong ... Transient transfection of RAW 264.7 cells with luciferase reporter constructs of the murine VEGF promoter indicates that both hypoxia and LPS/IFN-gamma independently induce VEGF promoter activity, irrespective of cell density ... Although LPS/IFN-gamma treatment induces transcriptional activation of the VEGF promoter, significant levels of VEGF protein are only expressed by cells at high density under conditions of pericellular hypoxia ... Deletion analysis of the VEGF promoter shows that the hypoxia response element region and its immediate flanking sequences are essential for both hypoxia and LPS/IFN-gamma induced VEGF promoter activation
Boiardi et al., J Rheumatol 2003 (Genetic Predisposition to Disease...) : Lipopolysaccharide (LPS) stimulated VEGF production by PBMC from controls was higher in II homozygous compared to DD homozygous patients
Tomaszewska et al., Gynecol Obstet Invest 2003 : The present study investigated the effect of 17beta-estradiol ( 17betaE ( 2 ) ) and lipopolysaccharide (LPS) on the release and expression of nitric oxide ( NO ), tumor necrosis factor-alpha (TNF-alpha) and vascular endothelial growth factor ( VEGF ) in the macrophage cell line RAW 264.7
Ming et al., Zhonghua Shao Shang Za Zhi 2004 : In contrary, LPS in high dose ( 50 and 100 micro g/ml ) could promote VEGF secretion ( P < 0.01 ) but exerted no effects on the proliferation of U937 cells ... U937 cells could be activated to increase the secretion of TGFbeta(1) by LPS in optimal dose of 0.1 - 10.0 micro g/ml, but the secretion of VEGF could only be promoted by LPS in higher concentration
Varney et al., J Interferon Cytokine Res 2005 (Melanoma...) : Further activation of monocytes and monocyte derived macrophages ( MDM ) by lipopolysaccharide (LPS) caused an increase in VEGF-A expression
Verhoeckx et al., Int Immunopharmacol 2006 : LPS in combination with beta2-agonists and cAMP elevating agents had an additional effect on the release of VEGF , OSM, and CXCL6
Bernardini et al., Cell Stress Chaperones 2005 : LPS stimulated VEGF secretion in a dose dependent manner ; its effect became significant after 7 hours and reached a plateau after 15 hours
Botero et al., J Endod 2006 : TLR4 mediates LPS induced VEGF expression in odontoblasts ... Lipopolysaccharide (LPS) from gram negative bacteria cell walls such as Prevotella intermedia and Escherichia coli induce vascular endothelial growth factor ( VEGF ) expression in odontoblasts, but not in undifferentiated dental pulp cells ... Dominant negative murine TLR4 ( DN-mTLR4 ) transfected MDPC-23 cells did not show upregulated VEGF expression in response to LPS stimulation ... These results demonstrate that odontoblast-like cells express CD14 and TLR4, and that LPS induced VEGF expression is mediated, at least in part, by TLR4 signaling
Ramanathan et al., Mol Biol Cell 2007 : We show here using VEGF promoter-luciferase reporter constructs that the TLR4 agonist Escherichia coli lipopolysaccharide (LPS) and the A2AR agonists NECA and CGS21680 synergistically augment VEGF transcription in macrophages and that the HRE in the VEGF promoter is essential for this transcription ... Deletion of putative NF-kappaB binding sites from the VEGF promoter did not affect LPS/NECA induced VEGF promoter activity, suggesting that NF-kappaB is not directly involved in VEGF transcription ... Taken together, these data indicate that LPS/NECA induced VEGF expression involves transcriptional regulation of the VEGF promoter by HIF-1alpha through the HRE
Xu et al., Zhongguo Zhong Yao Za Zhi 2006 : To investigate the effects of tetramethylpyrazine on lipopolysaccharides (LPS) induced expression of vascular endothelial growth factor ( VEGF ) and hypoxia induced factor-1alpha ( HIF-1alpha ) in macrophages
Poulaki et al., FASEB J 2007 (Leukostasis...) : 17-AAG treatment significantly suppressed the LPS induced increase in retinal leukocyte adhesion ; vascular leakage ; NF-kappaB, HIF-1alpha, p38, and PI-3K activity ; and VEGF , TNF-alpha, and IL-1beta levels
Oh et al., Neurosci Lett 2008 (Anoxia) : Moreover, HIF-1alpha knockdown by small interfering RNA ( siRNA ) decreased LPS induced expression of hypoxia responsive genes, VEGF , iNOS, and COX-2
Crisostomo et al., Am J Physiol Cell Physiol 2008 : TNF-alpha, LPS , and hypoxia significantly increased human MSC VEGF , FGF2, HGF, and IGF-1 production versus controls
Kim et al., Shock 2008 (Respiratory Distress Syndrome, Adult) : LPS induced vascular endothelial growth factor expression in rat lung pericytes ... LPS was found to enhance VEGF mRNA expression in a concentration dependent manner peaking 2 h after stimulation in pericytes ... Interestingly, attempted inhibition with nuclear factor-kappaB or tyrosine kinase did not suppress LPS induced augmented VEGF mRNA expression in lung pericytes, although both inhibitors markedly inhibited LPS induced iNOS mRNA expression ... SB203580, a p38 MAP kinase inhibitor, repressed LPS induced VEGF mRNA expression ... These results show that pericytes produce VEGF in response to LPS stimulation, and that this may be partly mediated by the p38 MAP kinase pathway
Pawlaczyk et al., Int J Artif Organs 2008 (Peritonitis) : LPS induced a dose dependent parallel increase of the intraperitoneal concentrations of MCP-1, IL-10 and TNF-alfa, as well as of VEGF
Heo et al., Immunol Lett 2008 (Inflammation) : Moreover, NOS inhibitor ( L-NAME ) and anti-TLR 4mAb reduced the LPS induced NO, IL-8 and VEGF production and ICAM-1 expression
Szczepanski et al., Cancer Res 2009 (Carcinoma, Squamous Cell...) : LPS binding to TLR4 on tumor cells enhanced proliferation, activated phosphatidylinositol 3-kinase/Akt pathway, up-regulated IRAK-4 expression, induced nuclear NF-kappaB translocation, and increased production ( P < 0.05 ) of interleukin (IL)-6, IL-8, vascular endothelial growth factor , and granulocyte macrophage colony stimulating factor
Ramanathan et al., J Leukoc Biol 2009 : This switch involves transcriptional regulation of VEGF by HIF-1, transcriptional induction of HIF-1alpha by LPS ( TLR4 agonist ), and A ( 2A ) R-dependent post-transcriptional regulation of HIF-1alpha stability
Kurago et al., Head and neck pathology 2008 (Carcinoma in Situ...) : In vitro, we used lipopolysaccharide (LPS) to model bacterial contamination, and evaluated the effects of oral and oropharyngeal ( O ) SCC-monocyte interactions and of LPS on OSCC cells and on the production of IL-6 and VEGF
Yao et al., J Biomed Sci 2009 (Myocardial Infarction) : LPS preconditioning enhanced survival of engrafted MSCs, stimulated expression of VEGF and activated PI3K/Akt pathway
Bernardini et al., Cell Stress Chaperones 2010 : LPS induced an increase in the level of VEGF in culture media after 7 and 15 h, and a larger increase was detected in CO pre treated cells
Pei et al., Int Immunopharmacol 2010 (Prostatic Neoplasms) : Sodium selenite inhibits the expression of VEGF , TGFbeta(1) and IL-6 induced by LPS in human PC3 cells via TLR4-NF-(K)B signaling blockage ... Using real-time quantitative PCR and ELISA analysis, we found that pretreatment with selenium ( 0.5-5uM ) inhibited the LPS induced expression of TGFbeta(1) and VEGF and production of these cytokines and IL-6 by PC3 cells, but did not alter the expression of TLR4 mRNA
Szajnik et al., Oncogene 2009 (Carcinoma...) : In MyD88 ( + ) SCOV3 cells, LPS or PTX binding to TLR4 induced IRAK4 activation and cJun phosphorylation, activated the NF-kappaB pathway and promoted interleukin (IL)-8, IL-6, vascular endothelial growth factor and monocyte chemotactic protein-1 production and resistance to drug induced apoptosis
Sharma et al., Fertil Steril 2010 (Endometriosis...) : The endometrial-endometriotic stromal cells were isolated from ectopic ( group I ) and eutopic ( group II ) endometrium by established methods, cultured, and stimulated with LPS ( 1 µg/mL ), followed by atorvastatin treatment in a time- and dose dependent manner to investigate the effects of LPS on proliferation ( Ki-67 ) and expression of cyclooxygenase-2 (COX-2), vascular endothelial growth factor ( VEGF ), receptor for advanced glycation end products ( RAGE ), extracellular newly identified RAGE binding protein ( EN-RAGE ), peroxisome proliferator activated receptor-? ( PPAR-? ), and liver X receptor-a ( LXR-a ) genes in endometrial-endometriotic stromal cells and on levels of insulin-like growth factor binding protein-1 ( IGFBP-1 ) and 17ß-E ( 2 ) in endometrial-endometriotic stromal cell culture supernatant
Botero et al., J Dent Res 2010 (Second Messenger Systems) : MAPK signaling is required for LPS induced VEGF in pulp stem cells ... Notably, LPS induced VEGF is associated with phosphorylation of protein kinase C ( PKC zeta ) and extracellular signal-regulator kinase ( ERK1/2 ) and is dependent upon MAPK activation
Miller et al., Am J Physiol Lung Cell Mol Physiol 2010 (Bronchopulmonary Dysplasia...) : Whereas LPS did not increase expression of VEGF , angiopoietin-1 (Ang-1), Tie2, fetal liver kinase-1 (Flk-1), fms-like tyrosine kinase-1 (Flt-1), PDGFA, PDGFB, heparin binding EGF-like growth factor ( HB-EGF ), or connective tissue growth factor (CTGF), LPS did stimulate the production of the angiogenic CC chemokines macrophage inflammatory protein-1a ( MIP-1a ) and monocyte chemoattractant protein-1 ( MCP-1 )
Tang et al., Am J Med Sci 2010 (Cell Transformation, Neoplastic...) : LPS promoted the production of vascular endothelial growth factor , transforming growth factor-beta, and interleukin-8
Westra et al., BMC musculoskeletal disorders 2010 (Arthritis...) : LPS stimulation in an hypoxic environment did not change VEGF levels, suggesting that LPS induced VEGF production in macrophages is more important than the hypoxic induction
Nikodemova et al., Neuroscience 2011 (Inflammation) : While LPS did not affect the expression of the growth factors VEGF or BDNF, we observed that mechanical injury ( caused by intraparenchymal injection ) induced distinct patterns of microglial activation characterized by increased expression of VEGF and down-regulation of BDNF
Ren et al., Am J Pathol 2011 (Bacterial Infections...) : Thus, LPS induced overexpression of VEGF in the biliary epithelium may lead to hypervascularity around the bile ducts ; concurrently, LPS and VEGF act as cell proliferation factors for cholangiocytes
Hirsch et al., Mediators Inflamm 2011 (Inflammation) : Consistent with this, 1,25-vit D ( 3 ) decreased LPS induced expression of macrophage inflammatory protein-1ß and VEGF in adult, but not neonatal, neutrophils
Tang et al., J Cell Mol Med 2013 (Inflammation...) : In addition, siRNA mediated knockdown of mLITAF and/or mSTAT6B leads to significant reduction in VEGF mRNA levels and inhibits LPS induced VEGF secretion in mouse RAW 264.7 cells
Iwata et al., PloS one 2013 (Intervertebral Disc Displacement...) : Further, lipopolysaccharide (LPS) induced expression of TNF-a, MMP3, MMP13, VEGF , and PGES mRNA in the 3D cultures, creating a molecular milieu that mimics that of degenerated NP
Xiong et al., Am J Pathol 1998 : In RAW cells, Ng-nitro-L-arginine-methyl ester and AG block IFNgamma/LPS activated, but not constitutive, VEGF production, whereas in MPMs, neither constitutive nor IFNgamma/LPS activated VEGF synthesis is affected