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RNA-seq Signal from REMC

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 mRNA-Seq  IMR90  IMR90  IMR90 mRNA Signal from REMC/UCSD    Data format 
    
Assembly: Human Feb. 2009 (GRCh37/hg19)

Vizhub @ Wash U built this track, and Roadmap Epigenomics Consortium is responsible for its contents.

Description

This track displays mRNA-Seq and smRNA-Seq data on a few cell types used in Roadmap Epigenomics Project. mRNA-Seq measures species and abundance of messanger RNA to estimate gene expression level. Such data is used in conjugation with epigenetic maps of corresponding samples to study relationship between epigenomics and gene expression regulation.

smRNA-Seq measures species and abundance of small RNAs. The small RNA has great implication in transcrptional regulation, and is considered to be important epigenetic mark.

Display conventions

This track displays read density data in form of wiggle plots. Number of aligned reads is counted at each base pair, and a summarized value is computed for each 20 bp interval for display. The subtracks can be displayed and configured individually. See instructions .

Methods

Experimental protocols: follow this link for experimental protocols.

Data processing: EDACC carried out data processing and quality assessment. Details are fully explained here . In brief, sequencing reads were aligned with 'Pash' program to derive read density data. The read density data is prepared into 'wiggle' format files with fixed step length of 20 bp. Data in wiggle and other formats have been deposited in NCBI Gene Expression Omnibus database for public access.

Release Notes

The data is combination of Release II, III, IV, V, VI, VII, VIII and IX which were mapped to human reference genome version hg19. The data is production of Roadmap Epigenomics Project.

Please follow the link for Roadmap Epigenomics data access policy

Credits

These data were generated in labs from UCSF and UBC as part of Roadmap Epigenomics Project.

Useful links