◀ Back to IL2

IL2 — LCK

Pathways - manually collected, often from reviews:

• BioCarta il 2 signaling pathway: IL-2/IL-2R-alpha/IL-2R-beta/IL-2R-gamma/JAK1/JAK3/LCK/SYK complex (IL2-IL2RA-JAK3-JAK1-SYK-IL2RG-IL2RB-LCK) → SHC/GRB2/SOS-1 complex (SOS1-GRB2-SHC1) (modification, activates)
  
• BioCarta il 2 signaling pathway: SYK → IL-2/IL-2R-alpha/IL-2R-beta/IL-2R-gamma/JAK1/JAK3/LCK/SYK complex (IL2-IL2RA-JAK3-JAK1-SYK-IL2RG-IL2RB-LCK) (modification, collaborate)
  
• BioCarta il 2 signaling pathway: IL-2/IL-2R-alpha/IL-2R-beta/IL-2R-gamma/JAK1/JAK3 complex (IL2RB-JAK1-IL2RA-IL2RG-JAK3-IL2) → LCK (modification, collaborate)
• BioCarta il 2 signaling pathway: IL-2/IL-2R-alpha/IL-2R-beta/IL-2R-gamma/JAK1/JAK3 complex (IL2RB-JAK1-IL2RA-IL2RG-JAK3-IL2) → IL-2/IL-2R-alpha/IL-2R-beta/IL-2R-gamma/JAK1/JAK3/LCK/SYK complex (IL2-IL2RA-JAK3-JAK1-SYK-IL2RG-IL2RB-LCK) (modification, collaborate)
  
• BioCarta il 2 signaling pathway: LCK → IL-2/IL-2R-alpha/IL-2R-beta/IL-2R-gamma/JAK1/JAK3/LCK/SYK complex (IL2-IL2RA-JAK3-JAK1-SYK-IL2RG-IL2RB-LCK) (modification, collaborate)
  
• NCI Pathway Database IL2-mediated signaling events: IL2R gamma/JAK3/SOCS1 complex (IL2RG-JAK3-SOCS1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, inhibits)
  Delespine-Carmagnat et al., FEBS Lett 1999, Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Arima et al., J Immunol 1991, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Sharon et al., Science 1986, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Miyazaki et al., Science 1994, Beadling et al., EMBO J 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Endo et al., Nature 1997, Liu et al., Curr Biol 1997, Zhou et al., Proc Natl Acad Sci U S A 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → IL2R alpha (IL2RA) (modification, collaborate) Delespine-Carmagnat et al., FEBS Lett 1999, Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Arima et al., J Immunol 1991, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Sharon et al., Science 1986, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Miyazaki et al., Science 1994, Beadling et al., EMBO J 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Endo et al., Nature 1997, Liu et al., Curr Biol 1997, Zhou et al., Proc Natl Acad Sci U S A 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK) (modification, collaborate)
  Delespine-Carmagnat et al., FEBS Lett 1999, Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Arima et al., J Immunol 1991, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Sharon et al., Science 1986, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Miyazaki et al., Science 1994, Beadling et al., EMBO J 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Endo et al., Nature 1997, Liu et al., Curr Biol 1997, Zhou et al., Proc Natl Acad Sci U S A 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → IL2 (IL2) (modification, collaborate) Delespine-Carmagnat et al., FEBS Lett 1999, Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Arima et al., J Immunol 1991, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Sharon et al., Science 1986, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Miyazaki et al., Science 1994, Beadling et al., EMBO J 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Endo et al., Nature 1997, Liu et al., Curr Biol 1997, Zhou et al., Proc Natl Acad Sci U S A 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → IL2R gamma/JAK3 complex (IL2RG-JAK3) (modification, collaborate)
  Delespine-Carmagnat et al., FEBS Lett 1999, Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Arima et al., J Immunol 1991, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Sharon et al., Science 1986, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Miyazaki et al., Science 1994, Beadling et al., EMBO J 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Endo et al., Nature 1997, Liu et al., Curr Biol 1997, Zhou et al., Proc Natl Acad Sci U S A 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2R beta/JAK1/LCK/SOCS3 complex (IL2RB-JAK1-LCK-SOCS3) → IL2 (IL2) (modification, inhibits) Delespine-Carmagnat et al., FEBS Lett 1999, Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Arima et al., J Immunol 1991, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Sharon et al., Science 1986, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Miyazaki et al., Science 1994, Beadling et al., EMBO J 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Endo et al., Nature 1997, Liu et al., Curr Biol 1997, Zhou et al., Proc Natl Acad Sci U S A 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2R beta/JAK1/LCK/SOCS1 complex (IL2RB-JAK1-LCK-SOCS1) → IL2 (IL2) (modification, inhibits) Delespine-Carmagnat et al., FEBS Lett 1999, Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Arima et al., J Immunol 1991, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Sharon et al., Science 1986, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Miyazaki et al., Science 1994, Beadling et al., EMBO J 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Endo et al., Nature 1997, Liu et al., Curr Biol 1997, Zhou et al., Proc Natl Acad Sci U S A 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK) → IL2 (IL2) (modification, collaborate) Delespine-Carmagnat et al., FEBS Lett 1999, Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Arima et al., J Immunol 1991, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Sharon et al., Science 1986, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Miyazaki et al., Science 1994, Beadling et al., EMBO J 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Endo et al., Nature 1997, Liu et al., Curr Biol 1997, Zhou et al., Proc Natl Acad Sci U S A 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2 (IL2) → IL2R beta/JAK1/LCK/CISH complex (IL2RB-JAK1-LCK-CISH) (modification, collaborate)
  Delespine-Carmagnat et al., FEBS Lett 1999, Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Arima et al., J Immunol 1991, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Sharon et al., Science 1986, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Miyazaki et al., Science 1994, Beadling et al., EMBO J 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Endo et al., Nature 1997, Liu et al., Curr Biol 1997, Zhou et al., Proc Natl Acad Sci U S A 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → Aiolos (IKZF3) (modification, activates) Romero et al., EMBO J 1999, Cacalano et al., Nat Cell Biol 2001, Satoh et al., J Biol Chem 1992, Graves et al., J Immunol 1992, Nelms et al., Immunity 1998
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → RasGAP/Dok-R complex (RASA1-DOK2) (modification, activates)
  Romero et al., EMBO J 1999, Cacalano et al., Nat Cell Biol 2001, Satoh et al., J Biol Chem 1992, Graves et al., J Immunol 1992, Nelms et al., Immunity 1998
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → SOCS3/RasGAP complex (SOCS3-RASA1) (modification, activates)
  Romero et al., EMBO J 1999, Cacalano et al., Nat Cell Biol 2001, Satoh et al., J Biol Chem 1992, Graves et al., J Immunol 1992, Nelms et al., Immunity 1998
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → None (modification, activates) Romero et al., EMBO J 1999, Cacalano et al., Nat Cell Biol 2001, Satoh et al., J Biol Chem 1992, Graves et al., J Immunol 1992, Nelms et al., Immunity 1998
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → RAS family/GDP/Aiolos complex (HRAS_KRAS_HRAS_KRAS_NRAS-IKZF3) (modification, activates)
  Romero et al., EMBO J 1999, Cacalano et al., Nat Cell Biol 2001, Satoh et al., J Biol Chem 1992, Graves et al., J Immunol 1992, Nelms et al., Immunity 1998
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → None (modification, activates) Romero et al., EMBO J 1999, Cacalano et al., Nat Cell Biol 2001, Satoh et al., J Biol Chem 1992, Graves et al., J Immunol 1992, Nelms et al., Immunity 1998
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → RAS family/GTP complex (HRAS_KRAS_HRAS_KRAS_NRAS) (modification, activates)
  Romero et al., EMBO J 1999, Cacalano et al., Nat Cell Biol 2001, Satoh et al., J Biol Chem 1992, Graves et al., J Immunol 1992, Nelms et al., Immunity 1998
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/PYK2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-PTK2B) (modification, collaborate)
  Miyazaki et al., Genes Dev 1998
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → PYK2 (PTK2B) (modification, collaborate) Miyazaki et al., Genes Dev 1998
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/PYK2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-PTK2B) → PYK2 (PTK2B) (modification, collaborate) Miyazaki et al., Genes Dev 1998
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database IL2 signaling events mediated by PI3K: None → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) (modification, collaborate)
  Augustine et al., Mol Cell Biol 1991, Gómez et al., J Immunol 1997
  Evidence: assay, other species
• NCI Pathway Database IL2 signaling events mediated by PI3K: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) → PKC zeta (PRKCZ) (modification, activates) Gómez et al., Exp Cell Res 1995, Gómez et al., J Immunol 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK) → IL2 (IL2) (modification, collaborate) Delespine-Carmagnat et al., FEBS Lett 1999, Tsujino et al., Genes Cells 1999, Tsujino et al., Proc Natl Acad Sci U S A 2000, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, González-García et al., J Biol Chem 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, collaborate)
  Burns et al., J Biol Chem 1993, Ravichandran et al., J Biol Chem 1994, Ravichandran et al., Proc Natl Acad Sci U S A 1996, Friedmann et al., Proc Natl Acad Sci U S A 1996, Lord et al., J Immunol 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1) → SHC (SHC1) (modification, collaborate) Burns et al., J Biol Chem 1993, Ravichandran et al., J Biol Chem 1994, Ravichandran et al., Proc Natl Acad Sci U S A 1996, Friedmann et al., Proc Natl Acad Sci U S A 1996, Lord et al., J Immunol 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → SHC (SHC1) (modification, collaborate) Burns et al., J Biol Chem 1993, Ravichandran et al., J Biol Chem 1994, Ravichandran et al., Proc Natl Acad Sci U S A 1996, Friedmann et al., Proc Natl Acad Sci U S A 1996, Lord et al., J Immunol 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2R beta/JAK1/LCK complex (IL2RB-JAK1-LCK) → IL2/IL2R beta/gamma/JAK1/LCK/JAK3 complex (IL2RB-JAK1-LCK-IL2-IL2RG-JAK3) (modification, collaborate)
  Delespine-Carmagnat et al., FEBS Lett 1999, Tsujino et al., Genes Cells 1999, Tsujino et al., Proc Natl Acad Sci U S A 2000, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, González-García et al., J Biol Chem 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2 (IL2) → IL2/IL2R beta/gamma/JAK1/LCK/JAK3 complex (IL2RB-JAK1-LCK-IL2-IL2RG-JAK3) (modification, collaborate)
  Delespine-Carmagnat et al., FEBS Lett 1999, Tsujino et al., Genes Cells 1999, Tsujino et al., Proc Natl Acad Sci U S A 2000, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, González-García et al., J Biol Chem 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2R gamma/JAK3 complex (IL2RG-JAK3) → IL2/IL2R beta/gamma/JAK1/LCK/JAK3 complex (IL2RB-JAK1-LCK-IL2-IL2RG-JAK3) (modification, collaborate)
  Delespine-Carmagnat et al., FEBS Lett 1999, Tsujino et al., Genes Cells 1999, Tsujino et al., Proc Natl Acad Sci U S A 2000, Takeshita et al., J Immunol 1992, Asao et al., FEBS Lett 1992, Takeshita et al., Science 1992, Hatakeyama et al., Science 1991, Asao et al., J Exp Med 1990, Mills et al., J Biol Chem 1990, Hatakeyama et al., Cell 1989, Goldsmith et al., J Biol Chem 1995, Boussiotis et al., Science 1994, Russell et al., Science 1994, Rebollo et al., Immunology 1993, Lai et al., Proc Natl Acad Sci U S A 1996, González-García et al., J Biol Chem 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R beta/gamma/JAK1/LCK/JAK3 complex (IL2RB-JAK1-LCK-IL2-IL2RG-JAK3) → PKC beta (PRKCB) (modification, activates) Gómez et al., Exp Cell Res 1995
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → Dok-R (DOK2) (modification, activates) Nelms et al., Immunity 1998
• NCI Pathway Database SHP2 signaling: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) → PI3K complex (PIK3CA-PIK3R1) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Gu et al., Mol Cell Biol 2000, Merida et al., J Immunol 1991, Truitt et al., J Biol Chem 1994, Kanazawa et al., Cell Immunol 1994, Gómez et al., J Immunol 1997
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database SHP2 signaling: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Gu et al., Mol Cell Biol 2000, Merida et al., J Immunol 1991, Truitt et al., J Biol Chem 1994, Kanazawa et al., Cell Immunol 1994, Gómez et al., J Immunol 1997
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database SHP2 signaling: PI3K complex (PIK3CA-PIK3R1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Gu et al., Mol Cell Biol 2000, Merida et al., J Immunol 1991, Truitt et al., J Biol Chem 1994, Kanazawa et al., Cell Immunol 1994, Gómez et al., J Immunol 1997
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database SHP2 signaling: RHOA/GTP complex (RHOA) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, activates)
  Gadina et al., J Biol Chem 2000, Gu et al., Mol Cell Biol 2000, Merida et al., J Immunol 1991, Truitt et al., J Biol Chem 1994, Kanazawa et al., Cell Immunol 1994, Gómez et al., J Immunol 1997
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: STAT1 (STAT1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, collaborate)
  Delespine-Carmagnat et al., Eur J Immunol 2000, Frank et al., Proc Natl Acad Sci U S A 1995, Hou et al., Immunity 1995
  Evidence: assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → STAT1 (dimer) complex (STAT1) (modification, activates)
  Delespine-Carmagnat et al., Eur J Immunol 2000, Frank et al., Proc Natl Acad Sci U S A 1995, Hou et al., Immunity 1995
  Evidence: assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/IRS1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-IRS2_IRS1) → PI3K complex (PIK3CA-PIK3R1) (modification, collaborate)
  Johnston et al., J Biol Chem 1995
  Evidence: physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/IRS1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-IRS2_IRS1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/IRS1-2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-IRS2_IRS1-PIK3CA-PIK3R1) (modification, collaborate)
  Johnston et al., J Biol Chem 1995
  Evidence: physical interaction
• NCI Pathway Database IL2-mediated signaling events: PI3K complex (PIK3CA-PIK3R1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/IRS1-2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-IRS2_IRS1-PIK3CA-PIK3R1) (modification, collaborate)
  Johnston et al., J Biol Chem 1995
  Evidence: physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) (modification, collaborate)
  Gu et al., Mol Cell Biol 2000, Zhu et al., J Biol Chem 1994, Ravichandran et al., J Biol Chem 1994
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1) → GAB2/GRB2/SOS1 complex (GRB2-SOS1-GAB2) (modification, collaborate)
  Gu et al., Mol Cell Biol 2000, Zhu et al., J Biol Chem 1994, Ravichandran et al., J Biol Chem 1994
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → GAB2/GRB2/SOS1 complex (GRB2-SOS1-GAB2) (modification, collaborate)
  Gu et al., Mol Cell Biol 2000, Zhu et al., J Biol Chem 1994, Ravichandran et al., J Biol Chem 1994
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: Erk1-2-active (MAPK3/MAPK1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Arnaud et al., J Immunol 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: Erk1-2-active (MAPK3/MAPK1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/ERK1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-MAPK3_MAPK1) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Arnaud et al., J Immunol 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: Erk1-2-active (MAPK3/MAPK1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Arnaud et al., J Immunol 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → SHP2 (PTPN11) (modification, collaborate) Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Arnaud et al., J Immunol 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/ERK1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-MAPK3_MAPK1) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Arnaud et al., J Immunol 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Arnaud et al., J Immunol 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: SHP2 (PTPN11) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/ERK1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-MAPK3_MAPK1) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Arnaud et al., J Immunol 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: SHP2 (PTPN11) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Arnaud et al., J Immunol 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/ERK1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-MAPK3_MAPK1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Arnaud et al., J Immunol 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2R beta/JAK1/LCK/SOCS3 complex (IL2RB-JAK1-LCK-SOCS3) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, inhibits)
  Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Delespine-Carmagnat et al., Eur J Immunol 2000, Gilmour et al., Proc Natl Acad Sci U S A 1995, Gaffen et al., Proc Natl Acad Sci U S A 1995, Johnston et al., Proc Natl Acad Sci U S A 1995, Hou et al., Immunity 1995, Lin et al., Immunity 1995, Fujii et al., Proc Natl Acad Sci U S A 1995, Wakao et al., EMBO J 1995, Beadling et al., EMBO J 1994, Friedmann et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Liu et al., Curr Biol 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2R beta/JAK1/LCK/SOCS1 complex (IL2RB-JAK1-LCK-SOCS1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, inhibits)
  Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Delespine-Carmagnat et al., Eur J Immunol 2000, Gilmour et al., Proc Natl Acad Sci U S A 1995, Gaffen et al., Proc Natl Acad Sci U S A 1995, Johnston et al., Proc Natl Acad Sci U S A 1995, Hou et al., Immunity 1995, Lin et al., Immunity 1995, Fujii et al., Proc Natl Acad Sci U S A 1995, Wakao et al., EMBO J 1995, Beadling et al., EMBO J 1994, Friedmann et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Liu et al., Curr Biol 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → STAT5 (STAT5A/STAT5B) (modification, activates) Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Delespine-Carmagnat et al., Eur J Immunol 2000, Gilmour et al., Proc Natl Acad Sci U S A 1995, Gaffen et al., Proc Natl Acad Sci U S A 1995, Johnston et al., Proc Natl Acad Sci U S A 1995, Hou et al., Immunity 1995, Lin et al., Immunity 1995, Fujii et al., Proc Natl Acad Sci U S A 1995, Wakao et al., EMBO J 1995, Beadling et al., EMBO J 1994, Friedmann et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Liu et al., Curr Biol 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → STAT5 (dimer)-active (STAT5B/STAT5A) (modification, activates)
  Cohney et al., Mol Cell Biol 1999, Aman et al., J Biol Chem 1999, Delespine-Carmagnat et al., Eur J Immunol 2000, Gilmour et al., Proc Natl Acad Sci U S A 1995, Gaffen et al., Proc Natl Acad Sci U S A 1995, Johnston et al., Proc Natl Acad Sci U S A 1995, Hou et al., Immunity 1995, Lin et al., Immunity 1995, Fujii et al., Proc Natl Acad Sci U S A 1995, Wakao et al., EMBO J 1995, Beadling et al., EMBO J 1994, Friedmann et al., Proc Natl Acad Sci U S A 1996, Gaffen et al., J Biol Chem 1996, Liu et al., Curr Biol 1997, Fujii et al., EMBO J 1998
  Evidence: mutant phenotype, assay, reporter gene, physical interaction, other species
• NCI Pathway Database IL2 signaling events mediated by PI3K: None → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) (modification, activates)
  Gu et al., Mol Cell Biol 2000, Lindemann et al., J Biol Chem 2003, Akbar et al., Eur J Immunol 1996, Ahmed et al., Proc Natl Acad Sci U S A 1997, Reif et al., J Biol Chem 1997
  Evidence: mutant phenotype, assay, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R beta/gamma/JAK1/LCK/JAK3/PI3K complex (IL2RB-JAK1-LCK-IL2-IL2RG-JAK3-PIK3CA-PIK3R1) → PI3K complex (PIK3CA-PIK3R1) (modification, collaborate)
  Tsujino et al., Genes Cells 1999, Merida et al., J Immunol 1991, Taichman et al., J Biol Chem 1993, González-García et al., J Biol Chem 1997
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R beta/gamma/JAK1/LCK/JAK3/PI3K complex (IL2RB-JAK1-LCK-IL2-IL2RG-JAK3-PIK3CA-PIK3R1) → IL2/IL2R beta/gamma/JAK1/LCK/JAK3 complex (IL2RB-JAK1-LCK-IL2-IL2RG-JAK3) (modification, collaborate)
  Tsujino et al., Genes Cells 1999, Merida et al., J Immunol 1991, Taichman et al., J Biol Chem 1993, González-García et al., J Biol Chem 1997
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: PI3K complex (PIK3CA-PIK3R1) → IL2/IL2R beta/gamma/JAK1/LCK/JAK3 complex (IL2RB-JAK1-LCK-IL2-IL2RG-JAK3) (modification, collaborate)
  Tsujino et al., Genes Cells 1999, Merida et al., J Immunol 1991, Taichman et al., J Biol Chem 1993, González-García et al., J Biol Chem 1997
  Evidence: mutant phenotype, physical interaction, other species
• NCI Pathway Database SHP2 signaling: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → SHP2 (PTPN11) (modification, collaborate) Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) → PI3K complex (PIK3CA-PIK3R1) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Gu et al., Mol Cell Biol 2000, Merida et al., J Immunol 1991, Truitt et al., J Biol Chem 1994, Kanazawa et al., Cell Immunol 1994, Gómez et al., J Immunol 1997, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Gu et al., Mol Cell Biol 2000, Merida et al., J Immunol 1991, Truitt et al., J Biol Chem 1994, Kanazawa et al., Cell Immunol 1994, Gómez et al., J Immunol 1997, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database IL2-mediated signaling events: PI3K complex (PIK3CA-PIK3R1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Gu et al., Mol Cell Biol 2000, Merida et al., J Immunol 1991, Truitt et al., J Biol Chem 1994, Kanazawa et al., Cell Immunol 1994, Gómez et al., J Immunol 1997, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database SHP2 signaling: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: RHOA/GTP complex (RHOA) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, activates)
  Gadina et al., J Biol Chem 2000, Gu et al., Mol Cell Biol 2000, Merida et al., J Immunol 1991, Truitt et al., J Biol Chem 1994, Kanazawa et al., Cell Immunol 1994, Gómez et al., J Immunol 1997, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database SHP2 signaling: SHP2 (PTPN11) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11) (modification, collaborate)
  Gadina et al., J Biol Chem 2000, Arnaud et al., Biochem J 2004, Gadina et al., J Immunol 1998, Gadina et al., J Immunol 1999
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2 signaling events mediated by STAT5: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) → STAT5 (dimer)-active (STAT5B/STAT5A) (transcription, activates)
  Moriggl et al., Immunity 1999, Brennan et al., Immunity 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: IRS1-2 (IRS2/IRS1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, collaborate)
  Johnston et al., J Biol Chem 1995
  Evidence: physical interaction
• NCI Pathway Database IL2-mediated signaling events: IRS1-2 (IRS2/IRS1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/IRS1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-IRS2_IRS1) (modification, collaborate)
  Johnston et al., J Biol Chem 1995
  Evidence: physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/IRS1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-IRS2_IRS1) (modification, collaborate)
  Johnston et al., J Biol Chem 1995
  Evidence: physical interaction
• NCI Pathway Database IL2-mediated signaling events: RAF1 (RAF1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) (modification, activates)
  Blanchard et al., Oncogene 2000, Lundin Brockdorff et al., Cytokine 2002, Karnitz et al., Mol Cell Biol 1995
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: SYK (SYK) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, collaborate)
  Zhou et al., Mol Cell Biol 2000, Jiang et al., Blood 2003, Minami et al., Immunity 1995
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: SYK (SYK) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SYK complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SYK) (modification, collaborate)
  Zhou et al., Mol Cell Biol 2000, Jiang et al., Blood 2003, Minami et al., Immunity 1995
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SYK complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SYK) (modification, collaborate)
  Zhou et al., Mol Cell Biol 2000, Jiang et al., Blood 2003, Minami et al., Immunity 1995
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1) → p38alpha-beta-active (MAPK14/MAPK11) (modification, activates)
  Hunt et al., J Biol Chem 1999, Crawley et al., J Biol Chem 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1) → p38alpha-beta (MAPK14/MAPK11) (modification, activates)
  Hunt et al., J Biol Chem 1999, Crawley et al., J Biol Chem 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SYK complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SYK) → PI3K complex (PIK3CA-PIK3R1) (modification, activates)
  Jiang et al., Blood 2003
  Evidence: mutant phenotype
• NCI Pathway Database IL2-mediated signaling events: None → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, collaborate)
  Gómez et al., J Immunol 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → RHOA/GTP complex (RHOA) (modification, activates) Gómez et al., J Immunol 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → RHOA/GDP complex (RHOA) (modification, activates) Gómez et al., J Immunol 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → None (modification, activates) Gómez et al., J Immunol 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: PP2A (PPP2R5D) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (positive regulation of cell-cell adhesion, collaborate)
  Brockdorff et al., Cytokine 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database SHP2 signaling: RAF1 (RAF1) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1) (modification, activates)
  Blanchard et al., Oncogene 2000, Lundin Brockdorff et al., Cytokine 2002, Karnitz et al., Mol Cell Biol 1995
  Evidence: mutant phenotype
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/STAM1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-STAM2_STAM) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, collaborate)
  Endo et al., FEBS Lett 2000, Takeshita et al., Immunity 1997
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/STAM1-2 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-STAM2_STAM) → STAM1-2 (STAM2/STAM) (modification, collaborate) Endo et al., FEBS Lett 2000, Takeshita et al., Immunity 1997
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → STAM1-2 (STAM2/STAM) (modification, collaborate) Endo et al., FEBS Lett 2000, Takeshita et al., Immunity 1997
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database IL2 signaling events mediated by PI3K: IL2/IL2R beta/gamma/JAK1/LCK/JAK3/PI3K complex (IL2RB-JAK1-LCK-IL2-IL2RG-JAK3-PIK3CA-PIK3R1) → BCL-X (BCL2L1) (transcription, activates) González-García et al., J Biol Chem 1997
  Evidence: mutant phenotype, other species
• NCI Pathway Database IL2-mediated signaling events: STAT3 (STAT3) → IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) (modification, collaborate)
  Delespine-Carmagnat et al., Eur J Immunol 2000, Fung et al., Cell Signal 2003, Nielsen et al., Eur J Immunol 1994, Frank et al., Proc Natl Acad Sci U S A 1995, Johnston et al., Proc Natl Acad Sci U S A 1995, Hou et al., Immunity 1995, Lin et al., Immunity 1995, Ng et al., J Biol Chem 1997
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database IL2-mediated signaling events: IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3 complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3) → MEK1-2 (MAP2K1/MAP2K2) (modification, activates) Delespine-Carmagnat et al., Eur J Immunol 2000, Fung et al., Cell Signal 2003, Nielsen et al., Eur J Immunol 1994, Frank et al., Proc Natl Acad Sci U S A 1995, Johnston et al., Proc Natl Acad Sci U S A 1995, Hou et al., Immunity 1995, Lin et al., Immunity 1995, Ng et al., J Biol Chem 1997
  Evidence: mutant phenotype, assay, physical interaction

Text-mined interactions from Literome

Cho et al., J Biol Chem 2000 : In addition, CD38 ligation resulted in an elevated tyrosine kinase activity of the CD38 associated Lck and ultimate activation of interleukin-2 gene transcription ... Furthermore, expression of a kinase-deficient Lck mutant suppressed interleukin-2 gene activation in a dose dependent manner
Brockdorff et al., Eur Cytokine Netw 2000 (Mycosis Fungoides) : Lck is involved in interleukin-2 induced proliferation but not cell survival in human T cells through a MAP kinase independent pathway
Taichman et al., Cytokine 1992 : CTLL-2 and HT-2 LCK- and LCK ( Y505F ) -transfected cells remained dependent on IL-2 for their growth and survival in culture despite the findings that ( i ) IL-2 specifically stimulated elevations in the activity of the endogenous p56-LCK in untransfected CTLL-2 cells without affecting the activities of the other SRC-like kinases in these cells ( p59-FYN, p62-YES ) and that ( ii ) IL-2 mediated regulation of p56-LCK correlated with IL-2-driven proliferation of these T cells
Caron et al., Mol Cell Biol 1992 : Additional studies revealed that all the mutations tested, including deletion of the Src homology 3 region, abrogated the enhancement of antigen triggered interleukin-2 production by F505 p56lck , thus implying more stringent requirements for augmentation of antigen responsiveness by F505 Lck
Granelli-Piperno et al., J Autoimmun 1992 : A member of the src gene family, the lymphocyte-specific protein tyrosine kinase, p56lck , has been implicated in IL-2 production
Einspahr et al., J Leukoc Biol 1992 : The present studies indicate that IL-2 induces a rapid ( < or = 1 min ) increase in the catalytic activity of p56lck , as measured by increases in protein tyrosine kinase activity in vitro
Satoh et al., J Biol Chem 1992 : A nonreceptor-type tyrosine kinase, Lck, is associated with IL-2 receptor beta subunit, and the binding of IL-2 to its receptor induces the activation of Lck
Mills et al., Int Immunol 1992 : Activation of the IL-2R leads to serine and threonine phosphorylation of the SRC tyrosine kinase family member, LCK , and an increase in LCK tyrosine kinase activity
Torigoe et al., Proc Natl Acad Sci U S A 1992 : When combined with the recent evidence that IL-2 regulates p56lck in T cells, these results indicate that some flexibility exists in the ability of various src-like PTKs to participate in IL-2 signal transduction mechanisms and raise the possibility that lineage-specific ( T-versus B-cell ) responses to IL-2 may be determined at least in part by the repertoire of src-like PTKs expressed in the cell
Torigoe et al., Leukemia 1992 : In contrast to IL-2 's effects on p56-LCK in T-cells, studies of an IL-2-responsive cell line of the B-cell lineage that lacks p56-LCK revealed that IL-2 specifically regulates the activity of the p53/56-LYN kinase
Fukushima et al., Cell Signal 2006 : Inhibition of the Lck/Shc interaction led to the loss of IL-2 promoter activation, confirming that the role of Shc in IL-2 production requires its interaction with Lck
Torigoe et al., Blood 1992 (Leukemia, Myeloid, Acute...) : Recently, it was reported that the IL-2R ( whose p75 beta-subunit shares sequence homology with a known murine IL-3R subunit and a common beta-subunit of the human IL-3R and granulocyte-macrophage colony stimulating factor [ GM-CSF ] receptors ) can physically associate with and regulate the activity of the SRC-family PTK, p56-LCK
Yamamoto et al., Princess Takamatsu symposia 1991 : Under the same conditions, normal Lck and Lyn could not stimulate IL-2 promoter
Horak et al., Proc Natl Acad Sci U S A 1991 : The ability of IL-2 to induce p56lck activation was found to be independent of the capacity of p56lck to associate with either CD4 or CD8
Pan et al., Blood 2012 (HIV Infections) : Instead, Nef triggers Lck dependent activation of TGN associated Ras-Erk signaling to promote the production of the T lymphocyte survival factor IL-2 and to enhance virus spread
Metcalfe et al., Cell Immunol 1994 : Rapamycin did not inhibit IL2 secretion induced by TCR/CD4/p56lck , emphasizing the specific action of FK506 and cyclosporin A
Tamura et al., J Immunol 1995 : The anti-CD3 stimulation did not cause Lck activation in either the Th1 or Th2 clone, although remarkable activation was induced in both clones following anti-CD4 stimulation, indicating that Lck activation was not required for either IL-2 or IL-4 production of Th cells
Taieb et al., J Biol Chem 1993 : We show that upon mitogenic stimulation with anti-IgM antibodies and interleukin-2 , specific mRNA for p56lck becomes detectable in B cells after 24 h of activation and is followed by an increase in p56lck protein expression on days 2 and 3
Eljaafari et al., Cell Immunol 1995 : Although activation of T cells with interleukin 2 (IL-2) results in the activation of p56lck , accumulating data support the notion that Lck does not play an essential role in mitogenic signal delivery from the IL-2R ... Since this src related PTK has been shown to enhance TCR/CD3 mediated T cell responsiveness, here we investigated whether activation of Lck by IL-2 could contribute to enhance TCR/CD3 mediated T cell functions ... This increase of T cell functions was correlated with IL-2 induced p56lck activation in both dose-response and time-course experiments ... Taken together these results strongly suggest that activation of Lck by IL-2 may play a role in regulating CD3 mediated T cell functions
Vitte-Mony et al., Mol Immunol 1994 : We show here, using IL-2 dependent human natural killer cell lines, that p56lck is regulated by IL-2 in two different ways : ( 1 ) IL-2 induces a rapid increase of p56lck kinase activity as assessed in vitro ; and ( 2 ) following IL-2 stimulation, p56lck undergoes phosphorylation on serine residues that is reflected by a modification of its electrophoretic mobility in SDS-PAGE ... Furthermore, dose response experiments, and blocking studies performed with anti-IL-2R alpha antibodies, indicated that binding of IL-2 to the IL-2R beta chain was sufficient to produce these modifications of p56lck
Nishio et al., Proc Soc Exp Biol Med 1994 : The increase of coprecipitated p56lck with anti-IL-2R beta antibody by the treatment with IL-2 suggested that the affinity of p56lck to IL-2R beta was increased by IL-2 in NK-rich cells
Musso et al., J Exp Med 1994 : In fact, IFN-gamma, but not IL-2 , efficiently blocked Lsk induction by IL-4 or IL-13
Sancho et al., J Immunol 1993 : Anti-TCR/CD3 stimulation of the TCR/CD3+ J32-3.2 cells resulted in a weak stimulation of both the phosphatidyl inositol and tyrosine kinase signal transduction pathways, as measured by changes in the level of free intracellular calcium, tyrosine phosphorylation of TCR-zeta, CD3-epsilon and ZAP-70, p56lck , or p59fyn tyrosine kinase activity and IL-2 gene activation
Bröker et al., Eur J Immunol 1994 (Cell Transformation, Viral) : Since IL-2 binding to its receptor activated only the CD4 bound fraction of p56lck, the IL-2 induced p56lck activity was diminished after long-term CD4 ligation
Watts et al., J Immunol 1993 : IL-2 stimulation of T lymphocytes induces sequential activation of mitogen activated protein kinases and phosphorylation of p56lck at serine-59
Takeuchi et al., J Biol Chem 1993 : Under the same conditions, Lck did not stimulate IL-2 promoter unless it was activated by mutation ... Csk, which phosphorylates tyrosine residues in the negative regulatory sites of Src family kinases, down-regulated Fyn- and Lck mediated stimulation of the serum response element and Fyn mediated enhancement of IL-2 promoter activity
Minami et al., EMBO J 1993 : Association of p56lck with IL-2 receptor beta chain is critical for the IL-2 induced activation of p56lck ... To examine the mechanisms underlying p56lck PTK activation by IL-2 , we established a mouse pro-B cell line, BAF-B03, expressing both IL-2R beta ( either the wild-type or mutant forms ) and mouse p56lck at high levels ... Intriguingly, BAF-B03 cells expressing an IL-2R beta chain which lacks a different cytoplasmic region, the ` serine-rich ' region, also fail to activate p56lck in response to IL-2
Baldari et al., J Biol Chem 1993 : Calcium dependent cyclosporin A-sensitive activation of the interleukin-2 promoter by p56lck ... The transcription factor NF-AT mediated, at least in part, the p56lck activation of IL-2 expression
Chung et al., J Immunol 1997 : The T cell hybridoma BI-141 has been previously used to dissect the roles of Lck in Ag-induced IL-2 production ... Here we demonstrate that BI-141 undergoes apoptosis in response to TCR stimulation using Ag or anti-TCR Abs. Using a panel of BI-141 transfectants expressing constitutively activated Lck ( F505 ) or phosphotyrosine binding ( K154F505 and C156F505 ) or kinase impaired ( R273F505 ) mutants, we assess the relative requirements for Lck in TCR mediated IL-2 production and apoptosis